scholarly journals Urinary Metals Concentrations and Biomarkers of Autoimmunity among Navajo and Nicaraguan Men

2020 ◽  
Vol 17 (15) ◽  
pp. 5263
Author(s):  
Madeleine Scammell ◽  
Caryn Sennett ◽  
Rebecca Laws ◽  
Robert Rubin ◽  
Daniel Brooks ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Outcome Data ◽  
Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Male Population ◽  
Inductively Coupled ◽  
The Us ◽  
Control And Prevention ◽  
Plasma Mass Spectrometry

Metals are suspected contributors of autoimmune disease among indigenous Americans. However, the association between metals exposure and biomarkers of autoimmunity is under-studied. In Nicaragua, environmental exposure to metals is also largely unexamined with regard to autoimmunity. We analyzed pooled and stratified exposure and outcome data from Navajo (n = 68) and Nicaraguan (n = 47) men of similar age and health status in order to characterize urinary concentrations of metals, compare concentrations with the US National Health and Nutrition Examination Survey (NHANES) male population, and examine the associations with biomarkers of autoimmunity. Urine samples were analyzed for metals via inductively coupled plasma mass spectrometry (ICP-MS) at the US Centers for Disease Control and Prevention. Serum samples were examined for antinuclear antibodies (ANA) at 1:160 and 1:40 dilutions, using an indirect immunofluorescence assay and for specific autoantibodies using enzyme-linked immunosorbent assay (ELISA). Logistic regression analyses evaluated associations of urinary metals with autoimmune biomarkers, adjusted for group (Navajo or Nicaraguan), age, and seafood consumption. The Nicaraguan men had higher urinary metal concentrations compared with both NHANES and the Navajo for most metals; however, tin was highest among the Navajo, and uranium was much higher in both populations compared with NHANES. Upper tertile associations with ANA positivity at the 1:160 dilution were observed for barium, cesium, lead, strontium and tungsten.

scholarly journals Determination of Essential and Toxic Elements in Cattle Blood: Serum vs Plasma

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 465 ◽  
Author(s):  
Diego Luna ◽  
Marta López-Alonso ◽  
Yolanda Cedeño ◽  
Lucas Rigueira ◽  
Víctor Pereira ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Toxic Elements ◽  
Serum Samples ◽  
Element Analysis ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Essential And Toxic Elements ◽  
High Concentrations

This study was designed to evaluate the influence of type of blood sample (serum or plasma) on essential and toxic element analysis in cattle. Paired plasma and serum samples (n = 20) were acid digested, and the concentrations of As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn. Mo, Ni, P, Pb, Sb, Se, Sr and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS). The study findings indicate that plasma and serum samples appear suitable and interchangeable for the determination of most of the essential and toxic elements in blood in cattle. The only exceptions are Cu and Se, the concentrations of which were significantly lower (40.9 and 29.9% respectively) in serum than in plasma. Some of the Cu in blood samples from bovine ruminants is known to be sequestered during clotting. However, further research on Se in ruminants and other animal species is warranted. Finally, the significantly higher Mn (9.9%) concentrations in serum than in plasma may have been caused by haemolysis of some samples. Special attention should be paid to preventing haemolysis of samples during collection and processing, in order to prevent overestimation of elements present at high concentrations inside erythrocytes (i.e., Fe, Mn and Zn).

A standard addition method to quantify serum lithium by inductively coupled plasma mass spectrometry

2021 ◽  
pp. 000456322110547
Author(s):  
Xiaoyu Fan ◽  
Qing Li ◽  
Ping Lin ◽  
Zhonggan Jin ◽  
Meizi Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Standard Reference Materials ◽  
Addition Method ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Target Values ◽  
Plasma Mass Spectrometry

Background Therapeutic monitoring of lithium (Li) is important because of its narrow therapeutic range and therapeutic index. Here, the authors present the evaluation of an accurate method for the determination of lithium in serum. Method Serum samples were diluted with 0.3% ultrapure nitric acid and were spiked with an internal standard germanium (Ge). The Li/Ge ratio was detected in He mode; we utilized standard addition method to quantify lithium in human serum. The new inductively coupled plasma mass spectrometry (ICP-MS) assay was characterized for linearity, specificity, imprecision, trueness, accuracy, and comparison. Results The correlation coefficients (r) of linearity were all > 0.9999. The specificity proved to be good. The total coefficients of variation (CV) were 1.11% and 0.49% for the two serum samples. The mean bias from target values of standard reference materials (SRM 956d) was −0.71% for Level I, −017% for Level II, and 2.20 for Level III. External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine (RELA) gave satisfied results for the new method. Comparison with the ion-selective electrode routine method got reasonable results. Conclusion This high accuracy method is an attractive alternative for lithium measurement and can be used as a candidate reference method to improve quality of serum lithium in China.

A candidate reference method for serum potassium measurement by inductively coupled plasma mass spectrometry

2017 ◽  
Vol 55 (10) ◽  
Author(s):  
Ying Yan ◽  
Bingqing Han ◽  
Jie Zeng ◽  
Weiyan Zhou ◽  
Tianjiao Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Serum Potassium ◽  
Inductively Coupled Plasma ◽  
Low Cost ◽  
Internal Standard ◽  
Reference Method ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

AbstractBackground:Potassium is an important serum ion that is frequently assayed in clinical laboratories. Quality assurance requires reference methods; thus, the establishment of a candidate reference method for serum potassium measurements is important.Methods:An inductively coupled plasma mass spectrometry (ICP-MS) method was developed. Serum samples were gravimetrically spiked with an aluminum internal standard, digested with 69% ultrapure nitric acid, and diluted to the required concentration. TheResults:The correlation coefficients between the measuredConclusions:The new ICP-MS method is specific, precise, simple, and low-cost, and it may be used as a candidate reference method for standardizing serum potassium measurements.

Determination of serum calcium levels by 42Ca isotope dilution inductively coupled plasma mass spectrometry

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
Bingqing Han ◽  
Menglei Ge ◽  
Haijian Zhao ◽  
Ying Yan ◽  
Jie Zeng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Serum Calcium ◽  
Isotope Dilution ◽  
Inductively Coupled Plasma ◽  
Reference Method ◽  
Detection Accuracy ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Abstract: Serum calcium level is an important clinical index that reflects pathophysiological states. However, detection accuracy in laboratory tests is not ideal; as such, a high accuracy method is needed.Methods:We developed a reference method for measuring serum calcium levels by isotope dilution inductively coupled plasma mass spectrometry (ID ICP-MS), usingResults:The relative expanded uncertainty of the sample concentration was 0.414% (k=2). The range of repeatability (within-run imprecision), intermediate imprecision (between-run imprecision), and intra-laboratory imprecision were 0.12%–0.19%, 0.07%–0.09%, and 0.16%–0.17%, respectively, for two of the serum samples. SRM909bI, SRM909bII, SRM909c, and GBW09152 were found to be within the certified value interval, with mean relative bias values of 0.29%, −0.02%, 0.10%, and −0.19%, respectively. The range of recovery was 99.87%–100.37%. Results obtained by ID ICP-MS showed a better accuracy than and were highly correlated with those of other reference methods.Conclusions:ID ICP-MS is a simple and accurate candidate reference method for serum calcium measurement and can be used to establish and improve serum calcium reference system in China.

scholarly journals Occupational Exposure to Metals and Serum Adiponectin Levels Among Shipyard Workers: A Cohort Study with Weighted Quantile Sum Regression Analyses

2021 ◽  
Author(s):  
Chen-Jung Wu ◽  
A-Chuan Ho ◽  
Shih-Ya Chen ◽  
Chih-Hong Pan ◽  
Hsiao-Chi Chuang ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Inductively Coupled Plasma ◽  
Joint Effect ◽  
Serum Adiponectin ◽  
Metal Exposure ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inductively Coupled ◽  
Increased Risk ◽  
Metal Levels ◽  
Plasma Mass Spectrometry

Abstract Background: The influence of welding-associated air pollutants on workers’ health is mainly regarded as a core issue in public health and occupational medicine. Previous studies have indicated that workers exposed to metal fumes had an increased risk of metabolic syndrome, which was correlated with decreased serum adiponectin levels. This study aimed to explore whether heavy metal exposure affects the concentration of adiponectin among welding workers. Methods: The study participants were recruited from a shipyard with 31 office workers and 100 welding workers in 2015. Urinary metal concentrations were measured by inductively coupled plasma–mass spectrometry. Serum adiponectin was measured by enzyme-linked immunosorbent assay. Inferential statistics on repeated measures were performed using generalized estimating equations (GEEs). A weighted quantile sum (WQS) regression model was conducted to examine the joint effect of the association of multimetal exposure with serum adiponectin. Results: After adjustment for all confounding variables through a GEE analysis, significantly negative associations of numerous urinary metals with serum adiponectin were detected in the welding workers, including Cr (β = -0.088; 95% CI: -0.148, -0.027), Mn (β = -0.174; 95% CI: -0.267, -0.081), Co (β = -0.094; 95% CI: -0.158, -0.029), Ni (β = -0.108; 95% CI: -0.208, -0.008), Cd (β = -0.067; 95% CI: -0.115, -0.018), and Pb (β = -0.089; 95% CI: -0.163, -0.015). The contributions of multiple urinary metal levels to serum adiponectin levels, determined individually by WQS regression, suggested that Pb was the greatest contributor. Conclusions: Welding workers exposed to heavy metals such as Pb, Cr, Co, Mn, Ni, and Cd might have reduced serum adiponectin levels and an increased risk of cardiovascular disease.

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