Levels of Trace Elements in Erythrocytes as Endocrine Disruptors in Obese and Nonobese Women with Polycystic Ovary Syndrome

Introduction: Polycystic ovary syndrome (PCOS) is one of the most commonly recognized endocrinopathies in women. The literature lacks clear data that allow any meaningful conclusions to be drawn about the influence of trace elements in erythrocytes on the biochemical parameters of PCOS. Materials and methods: This study was conducted among 47 women meeting the Rotterdam criteria for the diagnosis of polycystic ovary syndrome. The research groups included women with PCOS with different BMI values (body mass index): obese women with PCOS (PCOS with BMI ≥ 30, mean BMI index 35.4 ± 4.4 kg/m2), nonobese PCOS women (PCOS with BMI < 30, mean BMI index 25.2 ± 2.8 kg/m2), and healthy control group (CG) with a mean BMI of 23.57 ± 0.9 kg/m2. The contents of trace elements in erythrocytes were determined with an inductively coupled plasma atomic emission spectrometer. Results: The only trace element showing significant differences in concentration between the studied groups was nickel (Ni). The level of nickel in the obese women with PCOS (BMI ≥ 30) was significantly higher than in nonobese women (BMI < 30). The content of other trace elements in erythrocytes did not differ significantly between the studied groups. Several significant correlations were found within each of the studied PCOS groups: in the group of obese women, the content of zinc (Zn) in erythrocytes positively correlated with prolactin, the content of magnesium (Mg) positively correlated with testosterone, and the content of manganese (Mn) negatively correlated with thyroid-stimulating hormone. In the group of nonobese women, Zn content correlated positively with testosterone, Ni with luteinizing hormone (LH) and estradiol, and Mg negatively correlated with estradiol. Conclusions: The relationship between the level of trace elements and the level of hormones suggests that, in obese women with PCOS, nickel may play a role in inhibiting the processes of folliculogenesis and ovulation. Research on trace elements and their relationship to ovulatory cycles and the development of PCOS may contribute to reducing the consequences of PCOS and, therefore, should be extended.

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Smoking as a risk factor for the development of polycystic ovary syndrome

Ukrainian Journal of Modern Toxicological Aspects ◽

10.33273/2663-4570-87-3-56-61 ◽

2019 ◽

Vol 87 (3) ◽

pp. 56-61

Author(s):

S.V. Gunkov ◽

S.I. Reheda

Keyword(s):

Polycystic Ovary Syndrome ◽

Inductively Coupled Plasma ◽

Polycystic Ovary ◽

Statistical Significance ◽

Statistical Processing ◽

Serum Levels ◽

Control Group ◽

Ovary Syndrome ◽

Inductively Coupled ◽

Group I

ABSTRACT. Objective. To study the prevalence of smoking among women with polycystic ovary syndrome (PCOS), as well as to establish the relationship between intensity, duration of smoking and serum levels of manganese and nickel. Materials and methods. A questionnaire survey was performed in two groups of women: the control group (I) included 38 healthy women, and the second group included 52 women with PCOS. In both groups, serum levels of manganese and were determined using inductively coupled plasma mass spectrometry. Statistical processing of the results of the survey was conducted using Statistika 8 software program. The U-Mann-Whitney test was used to assess the statistical significance of the obtained results. Comparison of the prevalence of smoking in both groups was performed using the parameter of statistical significance of differences for two relative values. Spearman coefficient was used for correlation analysis. Results. In women with PCOS, significantly higher manganese (p = 0.0012) and nickel (p = 0.0000) serum levels were found. In group I, 3 women reported smoking, in group II — 13 women. Among women with PCOS, the prevalence of smoking was significantly higher (p = 0.0000). Both groups differ neither in duration (p = 0.9463) nor in intensity of smoking (p = 0.7366). Duration and intensity of smoking were not associated with serum manganese. The intensity of smoking also did not affect serum levels of nickel. A positive correlation was found between serum nickel and the duration of smoking. Authors believe that during smoking manganese directly penetrates cerebral structures via olfactory rout. Only its small portion penetrates the bloodstream. This explains the high toxicity of manganese. Conclusion. Among women with PCOS smoking is much more common, however, the intensity and duration of smoking do not differ from the control group. Serum manganese levels are associated neither with duration nor with the intensity of smoking. Serum nickel levels are affected by the duration of smoking, rather than by the intensity of smoking. Keywords: polycystic ovary syndrome, intensity of smoking, duration of smoking, manganese, nickel.

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Assessing the variability and predictability of adipokines (adiponectin, leptin, resistin and their ratios) in non-obese and obese women with anovulatory polycystic ovary syndrome

10.21203/rs.2.11525/v2 ◽

2019 ◽

Author(s):

Christian Obirikorang ◽

William K.B.A. Owiredu ◽

Sandra Adu-Afram ◽

Emmanuel Acheampong ◽

Evans Adu Asamoah ◽

...

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Discriminatory Power ◽

Control Group ◽

Obese Women ◽

Ovary Syndrome ◽

And Control

Abstract Objectives: To assess variability and predictability of adiponectin, leptin, resistin and their ratios in non-obese and obese women with anovulatory polycystic ovary syndrome (aPCOS). Results: A total of 52 ovulatory controls (mean age=31.63±4.88 years, BMI=25.33±2.68 kg/m3); 54 non-obese [mean age=32.11±4.25 years, BMI=25.72±2.95 kg/m3] and 50 obese women with aPCOS [mean age= 33.64±4.14 years, BMI=39.19±2.99 kg/m3] were recruited. The aPCOS group had lower adiponectin [13.0(10.49-16.59) vs 18.42(15.72-19.92) µg/ml, p<0.0001], adiponectin: leptin ratio (A:L) [0.60(0.35-0.88) vs 1.19(0.92-1.37), p<0.0001], and adiponectin: resistin ratio (A:R) [0.30(0.21-0.43) vs 0.42(0.32-0.62), p<0.0001] but a higher leptin [20.02(14.54-26.80) vs 16.17(14.51-18.36) ng/ml, p<0.0001] and leptin: resistin ratio (L:R) [0.53(0.37-0.82) vs 0.40(0.27-0.48), p<0.0001] compared to the controls. The obese aPCOS group had lower adiponectin [11.04(5.66-13.25) vs 14.18(11.04-18.02), p<0.0001 and 18.42(15.72-19.92) µg/ml, p<0.0001], A:L [0.36(0.27-0.44) vs 0.78(0.61-1.16), p<0.0001 and 1.19(0.92-1.37), p<0.0001], and A:R [0.24(0.17-0.38) vs 0.40(0.23-0.58), p<0.0001 and 0.42(0.32-0.62), p<0.0001] but a higher leptin [26.80(14.28-32.09) vs 17.95(14.86-21.26), p<0.05 and 16.17(14.51-18.36) ng/ml, p<0.0001] and L:R [0.63(0.46-1.03) vs 0.41(0.30-0.61), p<0.0001 and 0.40(0.27-0.48), p<0.0001] compared to the non-obese aPCOS and control group, respectively. A:L showed the best discriminatory power in predicting aPCOS (AUC=0.83), followed by adiponectin alone (AUC=0.79), L:R and leptin alone (both AUC=0.69). Resistin alone had the poorest discriminatory power (AUC=0.48).

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The level of adhesion molecules in polycystic ovary syndrome

Reproductive health of woman ◽

10.30841/2708-8731.2.2021.232555 ◽

2021 ◽

Vol 2 ◽

pp. 57-63

Author(s):

L.A. Mogylnytska

Keyword(s):

Endothelial Dysfunction ◽

Serum Level ◽

Polycystic Ovary Syndrome ◽

Adhesion Molecules ◽

Polycystic Ovary ◽

Control Group ◽

Obese Women ◽

Obese People ◽

Ovary Syndrome ◽

The Relationship

Polycystic ovary syndrome (PCOS) is characterized by dysfunction of the ovaries, hyperandrogenism and combination of metabolic disorders that increase the risk of developing cardiovascular diseases.The objective: of the investigation was to study the serum level of adhesion molecules (ICAM-1, VCAM-1 and E-selectin) in women with PCOS with different body weights as a marker of endothelial dysfunction, and their relationship with other factors of cardiovascular pathology.Materials and methods. 45 women with PCOS were examined, among whom a group of obese people – 23 women (age: 22,82±3,03 years, BMI 35,67±4,6) and a non-obese – 22 women (age: 22,81±2,03 years, BMI 22,35±2,16). The control group included 20 women aged 20,15±2,15 years, BMI 21,32±2,19. The serum level of ICAM-1, VCAM-1 and E-selectin was determined by the enzyme immunoassay. The significance of the differences of mean values was determined by the Student’s t-test. Correlation and multivariate regression analysis was used to assess the relationship between the studied factors,.Results. We revealed a significant increase of the serum level of adhesion molecules in obese and non-obese women with PCOS in comparison with women in the control group: ICAM-1 – +54,18%, +36,72%, respectively (p<0,0001), VCAM-1 – +50,76%, +36,33% respectively (p<0,0001), E-selectin – +40,24%, +29,56% respectively (p<0,0001). The concentration of these factors was higher in obese women with PCOS compared to non-obese women with PCOS: ICAM-I – +12,76% (p=0,04), VCAM-1 – +10,58% (p=0,009), E-selectin – +8,24% (p=0,03); p<0,05. The relationship was established between insulin content, HOMA and Caro index, lipid metabolism, hyperandrogenemia and an increase of serum level of adhesion molecules (p<0,01).Conclusions. As a result of our study, an increase of serum level of adhesion molecules in PCOS was revealed, both in combination with obesity and normal body weight, which may be a manifestation of endothelial dysfunction in this pathological condition. An increase in the content of ICAM-1, VCAM-1, E-selectin may be associated with insulin resistance, dyslipidemia and hyperandrogenism.

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Abnormal values of trace elements and blood metals in patients with metabolic syndrome, depending on its components

Terapevticheskii arkhiv ◽

10.26442/00403660.2019.10.000342 ◽

2019 ◽

Vol 91 (10) ◽

pp. 70-75

Author(s):

V I Podzolkov ◽

T V Korolyova ◽

M G Kudryavtseva

Keyword(s):

Metabolic Syndrome ◽

Trace Elements ◽

Inductively Coupled Plasma ◽

Average Duration ◽

Atomic Emission ◽

Control Group ◽

Positive Correlation ◽

Inductively Coupled

Aim. To study the level of “toxic” (Al, Cd, Pb) and “essential” (Fe, Cu, Zn, Co, Cr, Ni, and Se) trace elements (TE) and blood metals (BM) in patients with metabolic syndrome (MS), depending on its individual components. Materials and methods. 112 patients with MS were examined (45 men and 67 women; average age 61.4±7.2 years, average duration of MS 8.7±5.2 years). The study of BM was carried out by atomic emission spectrophotometry with inductively coupled plasma . Results and discussion. To study the amount of TE and BM in the group of MS patients,it was significantly higher levels of “toxic” MEs - Al, Cd, Pb - were revealed along with a decrease in the content of the “essential” Zn element as compared to the control group. At the same time, a pronounced positive correlation between the fact of the presence of MS and the level of “toxic” microelements - Al (r=0.71; p

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Serum levels of fibronectin in obese and non-obese women with polycystic ovary syndrome

Problems of Endocrinology ◽

10.14341/probl201662427-31 ◽

2016 ◽

Vol 62 (4) ◽

pp. 27-31 ◽

Cited By ~ 1

Author(s):

Mogylnytska A. Liliya

Keyword(s):

Endothelial Dysfunction ◽

Serum Level ◽

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Serum Levels ◽

Control Group ◽

Obese Women ◽

Ovary Syndrome ◽

Insulin Resistence ◽

Relationship Of

Aim — to investigate serum level of fibronectin in obese and non-obese women with polycystic ovary syndrome (PCOS) as a marker of endothelial dysfunction, and the relationship of that factor to the endothelium-dependent dilatation and other risk factors for cardiovascular disease.Material and methods. We study 62 women: 45 — with PCOS (23 obese women, 22 — non-obese) and 20 — a control group. Serum level of fibronectin was determined by ELISA. Endothelium-dependent dilatation was measured by test of Celermajer. Data was presented as mean ± SD.Results. We found an elevation of serum level of fibronectin in obese women with PCOS compared to control (258,12±48,47 and 196,34±46,68 pmol/ml respectively; р<0,05), in non-obese women compared to control (227,62±35,36 and 196,34±46,68 pmol/ml respectively, р<0,05). Serum level of fibronectin in obese was higher than non-obese women (258,12±48,47 и 227,62±35,36 pmol/ml respectively, р<0,05). Also, there are a correlation between the serum levels of fibronectin and BMI, HOMA index, lipid metabolism, androgens in PCOS women obese and non-obese (p<0,05).Conclusion. Serum level of fibronectin is elevated in obese and non-obese women with PCOS. Hyperandrogenemia, dyslipidemia, insulin resistence, obesity appears to be significant factor to contributing elevation of fibronectin. The revealed change of fibronectin could reflect an endothelial dysfunction in PCOS.

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Comparison of the umbilical cord Blood’s anti-Mullerian hormone level in the newborns of mothers with polycystic ovary syndrome (PCOS) and healthy mothers

Journal of Ovarian Research ◽

10.1186/s13048-019-0583-4 ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

Faezeh Tadaion Far ◽

Shahideh Jahanian Sadatmahalleh ◽

Saeideh Ziaei ◽

Anoshirvan Kazemnejad

Keyword(s):

Polycystic Ovary Syndrome ◽

Cord Blood ◽

Pregnant Women ◽

Umbilical Cord ◽

Polycystic Ovary ◽

Clinical Findings ◽

Control Group ◽

Obese Women ◽

Ovary Syndrome ◽

Significant Difference

Abstract Background Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases. At present, the cause of the disease is not fully understood, but many studies have shown that PCOS is associated with genetic and environmental factors. The present study aimed to assess the umbilical cord blood’s Anti-mullerian hormone (AMH) level in the newborns of mothers suffering from PCOS comparing to healthy mothers. Methods This prospective cohort study was conducted on 120 pregnant women with PCOS, who were selected through Rotterdam criteria, and 60 healthy pregnant women as the control group. The subjects in each group were divided into obese and non-obese mothers according to their body mass index (BMI) before pregnancy. The cord blood samples were collected from the offsprings on the time of childbirth. Finally, the circulating concentrations of AMH in both sexes of the newborns were determined by specific assays. Results The research results showed that the blood level of AMH was higher in the neonates of obese mothers with PCOS comparing to the controls (P < 0.001). Mean AMH level was higher in male neonates born from non-obese PCOS mothers than in the controls (P < 0.001); however, there was not a significant difference in the level of AMH in female neonates between these two groups (P = 0.264). Also the level of the above biomarker was higher in both sexes of the neonates belonging to obese PCOS mothers compared with the neonates born from non-obese PCOS mothers (P < 0.001). Conclusion(s) It can be said that the cord blood AMH level of neonates from obese women with PCOS is higher than that in the newborns of non-obese PCOS mothers. Further longitudinal studies are needed to confirm the clinical findings of the present research.

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Assessing the variability and predictability of adipokines (adiponectin, leptin and resistin and their ratios) in non-obese and obese women with polycystic ovary syndrome

10.21203/rs.2.11525/v1 ◽

2019 ◽

Author(s):

Christian Obirikorang ◽

William K.B.A. Owiredu ◽

Sandra Adu-Afram ◽

Emmanuel Acheampong ◽

Evans Adu Asamoah ◽

...

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Discriminatory Power ◽

Control Group ◽

Obese Women ◽

Ovary Syndrome ◽

Pcos Group

Abstract Objectives To assess the variability in adiponectin, leptin, and resistin between ovulatory women, and non-obese and obese women with polycystic ovary syndrome (PCOS). The study also explores the ratios of the adipokines and evaluated their predictability for PCOS. Results The PCOS group presented with lower adiponectin [13.0(10.49-16.59) vs 18.42(15.72-19.92) µg/ml, p<0.0001], adiponectin: leptin ratio [0.60(0.35-0.88) vs 1.19(0.92-1.37), p<0.0001], and adiponectin: resistin ratio [0.30(0.21-0.43) vs 0.42(0.32-0.62), p<0.0001] but a higher leptin [20.02(14.54-26.80) vs 16.17(14.51-18.36) ng/ml, p<0.0001] and leptin: resistin ratio [0.53(0.37-0.82) vs 0.40(0.27-0.48), p<0.0001] compared to the ovulatory group. The obese PCOS group presented with lower adiponectin [11.04(5.66-13.25) vs 14.18(11.04-18.02) µg/ml, p<0.0001 and 18.42(15.72-19.92) µg/ml, p<0.0001], adiponectin: leptin ratio [0.36(0.27-0.44) vs 0.78(0.61-1.16), p<0.0001 and 1.19(0.92-1.37), p<0.0001], and adiponectin: resistin ratio [0.24(0.17-0.38) vs 0.40(0.23-0.58), p<0.0001 and 0.42(0.32-0.62), p<0.0001] but a higher leptin [26.80(14.28-32.09) vs 17.95(14.86-21.26) ng/ml, p<0.05 and 16.17(14.51-18.36) ng/ml, p<0.0001] and leptin: resistin ratio [0.63(0.46-1.03) vs 0.41(0.30-0.61), p<0.0001 and 0.40(0.27-0.48), p<0.0001] compared to the non-obese PCOS and ovulatory control group, respectively. Adiponectin: leptin ratio presented with the best discriminatory power in predicting PCOS (AUC=0.83) followed by adiponectin alone (AUC=0.79), and leptin: resistin ratio and leptin alone (both AUC=0.69). Resistin alone presented with the poorest discriminatory power (AUC=0.48).

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The frequency and structure of metabolic disorders in obese women in combination with polycystic ovary syndrome

Obesity and metabolism ◽

10.14341/omet12374 ◽

2021 ◽

Vol 18 (3) ◽

pp. 254-262

Author(s):

E. V. Bolotova ◽

A. V. Dudnikova ◽

V. A. Krutova ◽

N. S. Prosolupova

Keyword(s):

Polycystic Ovary Syndrome ◽

Metabolic Disorders ◽

Polycystic Ovary ◽

Reproductive Age ◽

Control Group ◽

Obese Women ◽

Women Of Reproductive Age ◽

Ovary Syndrome ◽

Group 2 ◽

Group 1

Background: Obesity is considered a global epidemic and is one of the most significant medical and social problems. Research in recent years shows that in 25-45% of cases of obesity, polycystic ovary syndrome (PCOS) is detected. The influence of obesity on the pathogenesis of metabolic disorders in this category of patients remains controversialAims: to determine the frequency and structure of metabolic disorders in obese women in combination with polycystic ovary syndrome (PCOS).Materials and methods: A single-center cross-sectional sample survey of women of reproductive age was conducted. The study included clinical anthropometry with measurement of body weight, height, waist circumference (OT) and hip circumference (OB), followed by calculation of body mass index (BMI) and OT/OB ratio, and measurement of blood pressure (BP). A biochemical blood test was performed, the hormonal status was examined, and an ultrasound examination of the pelvic organs was performed.Results: A survey of 136 women of reproductive age was conducted. Group 1 included obese women without PCOS (59 patients), group 2 included obese women in combination with PCOS (45 patients), and the control group included 38 healthy women. Group 1 included obese women without PCOS (59 patients), and group 2 included obese women with PCOS (45 patients), in the control group — 38 healthy women. Among group 2 patients, dyslipidemia, visceral obesity, arterial hypertension, insulin resistance, hyperinsulinemia, and hyperuricemia were significantly more common (p<0,05). Correlations between BMI and triglycerides, testosterone and total cholesterol were found (p<0,05). Obese and PCOS patients had significantly higher levels of C-reactive protein(CRP), fibrinogen, anti-Müllerian hormone (AMH), and hepatic transaminases (p<0,05). Vitamin D deficiency was detected in 13,2% of patients, and insufficiency — in 22,7% of patients. There is a statistically significant correlation between the level of 25 (OH)D and indicators of BMI, follicle-stimulating hormone, luteinizing hormone (p<0,05), the level of AMH (p=0,008).Conclusions: A high frequency of metabolic disorders in obese women in combination with PCOS has been identified, which necessitates early screening, diagnosis and treatment of these disorders to strengthen reproductive health and prevent chronic non-communicable diseases.

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