Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering

Diplodia seriata, one of the major causal agents of Botryosphaeria dieback, spreads worldwide, causing cankers, leaf spots and fruit black rot in grapevine. Vitis rupestris is an American wild grapevine widely used for resistance and rootstock breeding and was found to be highly resistant to Botryosphaeria dieback. The defense responses of V. rupestris to D. seriata 98.1 were analyzed by RNA-seq in this study. There were 1365 differentially expressed genes (DEGs) annotated with Gene Ontology (GO) and enriched by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The DEGs could be allocated to the flavonoid biosynthesis pathway and the plant–pathogen interaction pathway. Among them, 53 DEGs were transcription factors (TFs). The expression levels of 12 genes were further verified by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The aggregation of proteins on the plasma membrane, formation variations in the cytoskeleton and plasmodesmata and hormone regulations revealed a declined physiological status in V. rupestris suspension cells after incubation with the culture filtrates of D. seriata 98.1. This study provides insights into the molecular mechanisms in grapevine cells’ response to D. seriata 98.1, which will be valuable for the control of Botryosphaeria dieback.

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Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine Botryosphaeria dieback

PROTOPLASMA ◽

10.1007/s00709-014-0716-y ◽

2014 ◽

Vol 252 (2) ◽

pp. 679-687 ◽

Cited By ~ 19

Author(s):

M. Bénard-Gellon ◽

S. Farine ◽

M. L. Goddard ◽

M. Schmitt ◽

E. Stempien ◽

...

Keyword(s):

Extracellular Proteins ◽

Diplodia Seriata ◽

Neofusicoccum Parvum ◽

Botryosphaeria Dieback

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Production of Phytotoxic Metabolites by Botryosphaeriaceae in Naturally Infected and Artificially Inoculated Grapevines

Plants ◽

10.3390/plants10040802 ◽

2021 ◽

Vol 10 (4) ◽

pp. 802

Author(s):

Pierluigi Reveglia ◽

Regina Billones-Baaijens ◽

Jennifer Millera Millera Niem ◽

Marco Masi ◽

Alessio Cimmino ◽

...

Keyword(s):

Fungal Pathogens ◽

Protocatechuic Acid ◽

Physiological Factors ◽

Diplodia Seriata ◽

Grapevine Trunk Diseases ◽

Botryosphaeria Dieback ◽

Trunk Diseases ◽

One Year ◽

Pathogen Dna

Grapevine trunk diseases (GTDs) are considered a serious problem to viticulture worldwide. Several GTD fungal pathogens produce phytotoxic metabolites (PMs) that were hypothesized to migrate to the foliage where they cause distinct symptoms. The role of PMs in the expression of Botryosphaeria dieback (BD) symptoms in naturally infected and artificially inoculated wood using molecular and analytical chemistry techniques was investigated. Wood samples from field vines naturally infected with BD and one-year-old vines inoculated with Diplodia seriata, Spencermartinsia viticola and Dothiorella vidmadera were analysed by cultural isolations, quantitative PCR (qPCR) and targeted LC-MS/MS to detect three PMs: (R)-mellein, protocatechuic acid and spencertoxin. (R)-mellein was detected in symptomatic naturally infected wood and vines artificially inoculated with D. seriata but was absent in all non-symptomatic wood. The amount of (R)-mellein detected was correlated with the amount of pathogen DNA detected by qPCR. Protocatechuic acid and spencertoxin were absent in all inoculated wood samples. (R)-mellein may be produced by the pathogen during infection to break down the wood, however it was not translocated into other parts of the vine. The foliar symptoms previously reported in vineyards may be due to a combination of PMs produced and climatic and physiological factors that require further investigation.

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Pruning debris of grapevine as a potential inoculum source of Diplodia seriata, causal agent of Botryosphaeria dieback

European Journal of Plant Pathology ◽

10.1007/s10658-015-0782-9 ◽

2015 ◽

Vol 144 (4) ◽

pp. 803-810 ◽

Cited By ~ 5

Author(s):

Georgina Elena ◽

Jordi Luque

Keyword(s):

Causal Agent ◽

Diplodia Seriata ◽

Inoculum Source ◽

Botryosphaeria Dieback

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Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum

Frontiers in Microbiology ◽

10.3389/fmicb.2021.726132 ◽

2021 ◽

Vol 12 ◽

Author(s):

Catarina Leal ◽

Nicolas Richet ◽

Jean-François Guise ◽

David Gramaje ◽

Josep Armengol ◽

...

Keyword(s):

Bacillus Subtilis ◽

Trichoderma Atroviride ◽

In Planta ◽

Neofusicoccum Parvum ◽

Beneficial Effects ◽

Grapevine Trunk Diseases ◽

Mutual Antagonism ◽

Direct Benefits ◽

Botryosphaeria Dieback

Grapevine trunk diseases (GTDs) are a big threat for global viticulture. Without effective chemicals, biocontrol strategies are developed as alternatives to better cope with environmental concerns. A combination of biological control agents (BCAs) could even improve sustainable disease management through complementary ways of protection. In this study, we evaluated the combination of Bacillus subtilis (Bs) PTA-271 and Trichoderma atroviride (Ta) SC1 for the protection of Chardonnay and Tempranillo rootlings against Neofusicoccum parvum Bt67, an aggressive pathogen associated to Botryosphaeria dieback (BD). Indirect benefits offered by each BCA and their combination were then characterized in planta, as well as their direct benefits in vitro. Results provide evidence that (1) the cultivar contributes to the beneficial effects of Bs PTA-271 and Ta SC1 against N. parvum, and that (2) the in vitro BCA mutual antagonism switches to the strongest fungistatic effect toward Np-Bt67 in a three-way confrontation test. We also report for the first time the beneficial potential of a combination of BCA against Np-Bt67 especially in Tempranillo. Our findings highlight a common feature for both cultivars: salicylic acid (SA)-dependent defenses were strongly decreased in plants protected by the BCA, in contrast with symptomatic ones. We thus suggest that (1) the high basal expression of SA-dependent defenses in Tempranillo explains its highest susceptibility to N. parvum, and that (2) the cultivar-specific responses to the beneficial Bs PTA-271 and Ta SC1 remain to be further investigated.

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First Report of Wood Canker Caused by Botryosphaeria dothidea, Diplodia seriata, Neofusicoccum parvum, and Lasiodiplodia theobromae on Grapevine in Turkey

Plant Disease ◽

10.1094/pdis-07-13-0726-pdn ◽

2014 ◽

Vol 98 (4) ◽

pp. 568-568 ◽

Cited By ~ 8

Author(s):

D. S. Akgul ◽

N. G. Savas ◽

A. Eskalen

Keyword(s):

Morphological Characteristics ◽

Botryosphaeria Dothidea ◽

Outer Bark ◽

Western Turkey ◽

Diplodia Seriata ◽

Neofusicoccum Parvum ◽

Lasiodiplodia Theobromae ◽

First Report ◽

Multiple Species ◽

Botryosphaeriaceae Species

The Aegean region (western Turkey) is the center of table, raisin, and wine grape cultivation. During the 2012 growing season, wood canker symptoms were observed in vineyards in Manisa city. Symptoms adjacent to pruning wounds, including shoot dieback and wedge-shaped wood discolorations observed in cross section, were among the most prevalent symptoms of the vines. To identify the causal agents, symptomatic woody tissues were surface disinfested with 95% ethanol and flame-sterilized and the discolored outer bark was cut away. The internal tissues (0.5 cm2) were excised from cankers of vines and plated onto potato dextrose agar amended with tetracycline (0.01%) (PDA-tet). The most frequently isolated fungi, based on general growth pattern, speed of growth, and colony color, resembled species in the Botryosphaeriaceae family. According to morphological characteristics, four different groups have been identified based on visual discrimination. After DNA extraction, ribosomal DNA fragments (ITS1-5.8S-ITS2) (2) amplified with ITS4 and ITS5 primers were sequenced and sequences were compared with those deposited in NCBI GenBank database. Four different Botryosphaeriaceae isolates were identified, including Botryosphaeria dothidea (MBAi25AG), Diplodia seriata (MBAi23AG), Lasiodiplodia theobromae (MBAi28AG), and Neofusicoccum parvum (MBAi27AG) (Accession Nos. KF182329, KF182328, KF182331, and KF182330, respectively) with species nomenclature based on Crous et al. (1). Pathogenicity tests were conducted under greenhouse conditions (24°C, 16/8-h day/night, 70% RH) on 1-year-old own rooted grapevine (Vitis vinifera) cv. Sultana Seedless seedlings using one isolate from each of the Botryosphaeriaceae species specified above. Stems of grapevine seedlings were wounded by removing bark with 4-mm cork borer and fresh mycelial plugs were inoculated into the holes and covered with Parafilm. Sterile PDA plugs were placed into the wounds of control seedlings. Five vines were inoculated per isolate. The experiment was repeated twice. After 4 months of incubation, grapevine seedlings were examined for the extent of vascular discoloration and recovery of fungal isolates. Mean lesion lengths on wood tissues were 85.3, 17.2, 13.9, and 13.1 mm for N. parvum, B. dothidea, L. theobromae, and D. seriata, and 6.3 mm for control. Each fungal isolate was successfully re-isolated from inoculated seedlings to fulfill Koch's postulates. To our knowledge, this is the first report of multiple species in the Botryosphaeriaceae causing wood canker and dieback on grapevine in Turkey. These results are significant because Botryosphaeriaceae species are known causal agents of grapevine trunk disease worldwide (3). References: (1) P. W. Crous et al. Stud. Mycol. 55:235, 2006. (2) B. Slippers et al. Mycologia 96:83, 2004. (3) J. R. Urbez-Torres. Phytopathol. Mediterr. 50:S5, 2011.

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Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

Phytochemistry ◽

10.1016/j.phytochem.2015.01.012 ◽

2015 ◽

Vol 115 ◽

pp. 207-215 ◽

Cited By ~ 49

Author(s):

Eliane Abou-Mansour ◽

Jean-Luc Débieux ◽

Montserrat Ramírez-Suero ◽

Mélanie Bénard-Gellon ◽

Maryline Magnin-Robert ◽

...

Keyword(s):

Neofusicoccum Parvum ◽

Botryosphaeria Dieback

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Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and Streptomyces spp. Secondary Metabolites against Three Botryosphaeriaceae Species

Antibiotics ◽

10.3390/antibiotics8030099 ◽

2019 ◽

Vol 8 (3) ◽

pp. 99 ◽

Cited By ~ 2

Author(s):

Laura Buzón-Durán ◽

Jesús Martín-Gil ◽

Eduardo Pérez-Lebeña ◽

David Ruano-Rosa ◽

José L. Revuelta ◽

...

Keyword(s):

Cost Effective ◽

Botryosphaeria Dothidea ◽

Diplodia Seriata ◽

Neofusicoccum Parvum ◽

Grapevine Trunk Diseases ◽

Streptomyces Spp ◽

Effective Option ◽

Trunk Diseases ◽

Botryosphaeriaceae Species

Grapevine trunk diseases (GTDs) are a major threat to the wine and grape industry. The aim of the study was to investigate the antifungal activity against Neofusicoccum parvum, Diplodia seriata, and Botryosphaeria dothidea of ε-polylysine, chitosan oligomers, their conjugates, Streptomyces rochei and S. lavendofoliae culture filtrates, and their binary mixtures with chitosan oligomers. In vitro mycelial growth inhibition tests suggest that the efficacy of these treatments, in particular those based on ε-polylysine and ε-polylysine:chitosan oligomers 1:1 w/w conjugate, against the three Botryosphaeriaceae species would be comparable to or higher than that of conventional synthetic fungicides. In the case of ε-polylysine, EC90 values as low as 227, 26.9, and 22.5 µg·mL−1 were obtained for N. parvum, D. seriata, and B. dothidea, respectively. Although the efficacy of the conjugate was slightly lower, with EC90 values of 507.5, 580.2, and 497.4 µg·mL−1, respectively, it may represent a more cost-effective option to the utilization of pure ε-polylysine. The proposed treatments may offer a viable and sustainable alternative for controlling GTDs.

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Identification and pathogenicity of Diplodia, Neofusicoccum, Cadophora and Diaporthe species associated with cordon dieback in kiwifruit cv. Hayward in central Chile

Plant Disease ◽

10.1094/pdis-05-20-0988-re ◽

2020 ◽

Author(s):

Gonzalo A Díaz ◽

Juan Pablo Zoffoli ◽

Enrique Ferrada ◽

Mauricio A. Lolas

Keyword(s):

Elongation Factor ◽

Its Region ◽

Fruit Trees ◽

Economic Losses ◽

Translation Elongation ◽

Diplodia Seriata ◽

Neofusicoccum Parvum ◽

Central Chile ◽

Field Surveys ◽

Dieback Disease

Dieback symptoms associated with fungal trunk pathogens cause significant economic losses to kiwifruit and other woody fruit trees worldwide. This study represents the first attempt to identify and characterize the fungal trunk pathogens associated with cordon dieback disease of kiwifruit in central Chile. Field surveys were conducted throughout the main kiwifruit-growing regions in central Chile to determine the incidence and to characterize the fungal trunk pathogens associated with cordon dieback of kiwifruit cv. Hayward through morphological, molecular and pathogenicity studies. A total of 250 cordon samples were collected, and the isolations were performed on acidified potato dextrose agar (2%, APDA) plus antibiotics and Igepal. The incidence of kiwifruit cordon dieback ranged between 5 and 85% in all surveyed areas in central Chile. A total of 246 isolates were isolated and identified using culture and morphological features as belonging to three fungal taxa. Diaporthaceae spp. (Diaporthe ambigua and D. australafricana; n=133 isolates), Botryosphaeriaceae spp. (Diplodia seriata and Neofusicoccum parvum; n=89 isolates) and Ploettnerulaceae spp. (Cadophora luteo-olivacea and C. malorum; n=24 isolates) were identified using phylogenetics studies of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rDNA, part of the β-tubulin gene (tub2) and part of the translation elongation factor 1-α gene (tef1-α). Isolates of N. parvum and Di. seriata were the most virulent, causing internal brown lesion and dieback symptoms in attached green shoots, attached lignified canes and young inoculated kiwifruits. This report is the first to describe Di. seriata and C. luteo-olivacea associated with kiwifruit cordon dieback in Chile and presents the first description of N. parvum causing kiwifruit dieback worldwide.

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