Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis

Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.

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MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Animals ◽

10.3390/ani10030468 ◽

2020 ◽

Vol 10 (3) ◽

pp. 468 ◽

Cited By ~ 4

Author(s):

Xiangfei Ma ◽

Junwei Sun ◽

Shuaipeng Zhu ◽

Zhenwei Du ◽

Donghua Li ◽

...

Keyword(s):

Production Efficiency ◽

Target Genes ◽

Strong Association ◽

Abdominal Fat ◽

Signal Pathway ◽

Fat Deposition ◽

Differentially Expressed ◽

Receptor Interaction ◽

Signal Pathways ◽

Abdominal Fat Deposition

The excessive deposition of abdominal fat has become an important factor in restricting the production efficiency of chickens, so reducing abdominal fat deposition is important for improving growth rate. It has been proven that miRNAs play an important role in regulating many physiological processes of organisms. In this study, we constructed a model of adipogenesis by isolating preadipocytes (Ab-Pre) derived from abdominal adipose tissue and differentiated adipocytes (Ab-Ad) in vitro. Deep sequencing of miRNAs and mRNAs expressed in Ab-Pre and Ab-Ad groups was conducted to explore the effect of miRNAs and mRNAs on fat deposition. We identified 80 differentially expressed miRNAs (DEMs) candidates, 58 of which were up-regulated and 22 down-regulated. Furthermore, six miRNAs and six mRNAs were verified by qRT-PCR, and the results showed that the expression of the DEMs and differentially expressed genes (DEGs) in the two groups was consistent with our sequencing results. When target genes of miRNA were combined with mRNA transcriptome data, a total of 891 intersection genes were obtained, we predicted the signal pathways of cross genes enrichment to the MAPK signal pathway, insulin signal pathway, fatty acid metabolism, and ECM–receptor interaction. Meanwhile, we constructed miRNA and negatively correlated mRNA target networks, including 12 miRNA–mRNAs pairs, which showed a strong association with the abdominal adipocyte differentiation (miR-214−ACSBG2, NFKB2, CAMK2A, ACLY, CCND3, PLK3, ITGB2; miR-148a-5p−ROCK2; miR-10a-5p−ELOVL5; miR-146b-5p−LAMA4; miR-6615-5p−FLNB; miR-1774−COL6A1). Overall, these findings provide a background for further research on lipid metabolism. Thus, we can better understand the molecular genetic mechanism of chicken abdominal fat deposition.

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Proinflammatory cytokines and cardiac abnormalities in uncomplicated obesity: Relationship with abdominal fat deposition

Nutrition Metabolism and Cardiovascular Diseases ◽

10.1016/j.numecd.2006.01.001 ◽

2007 ◽

Vol 17 (4) ◽

pp. 294-302 ◽

Cited By ~ 67

Author(s):

Alexis E. Malavazos ◽

Massimiliano M. Corsi ◽

Federica Ermetici ◽

Calin Coman ◽

Francesco Sardanelli ◽

...

Keyword(s):

Proinflammatory Cytokines ◽

Abdominal Fat ◽

Fat Deposition ◽

Cardiac Abnormalities ◽

Abdominal Fat Deposition

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Error in Acknowledgments in: Selective Protection Against Extremes in Childhood Body Size, Abdominal Fat Deposition, and Fat Patterning in Breastfed Children

Archives of Pediatrics and Adolescent Medicine ◽

10.1001/archpediatrics.2012.1232 ◽

2012 ◽

Vol 166 (7) ◽

pp. 607

Keyword(s):

Body Size ◽

Abdominal Fat ◽

Fat Deposition ◽

Selective Protection ◽

Abdominal Fat Deposition

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RNA-Seq Analysis Reveals Hub Genes Involved in Chicken Intramuscular Fat and Abdominal Fat Deposition During Development

Frontiers in Genetics ◽

10.3389/fgene.2020.01009 ◽

2020 ◽

Vol 11 ◽

Author(s):

Siyuan Xing ◽

Ranran Liu ◽

Guiping Zhao ◽

Lu Liu ◽

Martien A. M. Groenen ◽

...

Keyword(s):

Intramuscular Fat ◽

Abdominal Fat ◽

Fat Deposition ◽

Rna Seq ◽

Hub Genes ◽

Abdominal Fat Deposition

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Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model

Frontiers in Genetics ◽

10.3389/fgene.2018.00262 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 9

Author(s):

Bahareldin A. Abdalla ◽

Jie Chen ◽

Qinghua Nie ◽

Xiquan Zhang

Keyword(s):

Abdominal Fat ◽

Fat Deposition ◽

Multiple Factors ◽

Abdominal Fat Deposition ◽

Chicken Model

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mRNA expression of genes related to fat deposition during in vitro ovine adipogenesis

Canadian Journal of Animal Science ◽

10.1139/cjas-2018-0107 ◽

2019 ◽

Vol 99 (4) ◽

pp. 764-771 ◽

Cited By ~ 1

Author(s):

Baojun Li ◽

Liying Qiao ◽

Xiaoru Yan ◽

Tao Shi ◽

Duanyang Ren ◽

...

Keyword(s):

Mrna Expression ◽

Binding Protein ◽

Adipogenic Differentiation ◽

Fat Deposition ◽

Peroxisome Proliferator ◽

Induced Differentiation ◽

Fatty Acid Binding ◽

Acid Binding Protein ◽

Ppar Γ

Fat deposition in animals involves adipogenic differentiation guided by transcriptional factors and other key factors. To understand the molecular mechanism underlying ovine adipogenic differentiation, the dynamic mRNA expression of key genes related to fat deposition, including peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid-binding protein 4 (FABP4), FABP5, and cellular retinoic acid-binding protein 2 (CRABP2), were analyzed during in vitro differentiation of ovine preadipocytes. The stromal vascular cells from underneath the tail fat tissue of 1-wk-old sheep were isolated and cultured, and the preadipocytes were induced using a cocktail of 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and troglitazone. The cultivated cells were collected at different time points after induced differentiation. The expression levels of PPAR-γ, FABP4, FABP5, and CRABP2 were studied by quantitative real-time polymerase chain reaction. The expressions of these genes in sheep were compared with those in human and mouse retrieved from the Gene Expression Omnibus DataSets. We observed that the expression of PPAR-γ, FABP4, and FABP5 was increased upon differentiation of ovine preadipocytes, as in humans and mice. The expression of CRABP2 was sharply increased from days 0 to 2 after induced differentiation and was subsequently decreased. This expression pattern of CRABP2 was different from that observed in humans and mice. Our results provide new insights into the function of these genes in fat deposition.

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Dietary habits and physical activity: Which influence on abdominal fat deposition in children and adolescents?

Mediterranean Journal of Nutrition and Metabolism ◽

10.3233/mnm-200407 ◽

2020 ◽

Vol 13 (3) ◽

pp. 215-223

Author(s):

Rachele De Giuseppe ◽

Valeria Calcaterra ◽

Ginevra Biino ◽

Noelia Rodriguez ◽

Anna Gerbaldo ◽

...

Keyword(s):

Physical Activity ◽

Abdominal Obesity ◽

Dietary Habits ◽

Cross Sectional Study ◽

Abdominal Fat ◽

Normal Weight ◽

Fat Deposition ◽

Cross Sectional ◽

Validated Questionnaire ◽

Abdominal Fat Deposition

BACKGROUND: Prevention of childhood obesity is recommended throughout interventions aiming at enhancing healthy dietary habits (DH) and increasing physical activity (PA). OBJECTIVE: This cross-sectional study aimed at investigating DH and PA, in a sample of Italian children/adolescents. METHODS: We evaluated DH and PA by means of a previously validated questionnaire in 178 children/adolescents (88F/90M, 11.8±2.6 years): 49 normal weight (NW), 76 overweight (OW) and 53 with obesity (OB). Abdominal obesity was defined as Waist to Height Ratio (WHeR) >0.5. RESULTS: DH did not differ in NW subjects compared to OW and OB but higher PA score (p = 0.001) was observed in NW than OW and OB. No association was found between DH and WHeR, unlike PA. Four clusters of subjects were identified that could be defined by DH and PA; only one cluster, defined by PA, showed a significantly lower WHeR within subjects playing sports at least 3–4 hours/week. Moreover, subjects belonging to this cluster did not skip the main meals, eat a varied diet and drank milk at breakfast. CONCLUSIONS: In our sample PA performed at least 3–4 hours/week is inversely associated with abdominal fat deposition and is expected to positively impact health, protecting from abdominal obesity.

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