MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

The excessive deposition of abdominal fat has become an important factor in restricting the production efficiency of chickens, so reducing abdominal fat deposition is important for improving growth rate. It has been proven that miRNAs play an important role in regulating many physiological processes of organisms. In this study, we constructed a model of adipogenesis by isolating preadipocytes (Ab-Pre) derived from abdominal adipose tissue and differentiated adipocytes (Ab-Ad) in vitro. Deep sequencing of miRNAs and mRNAs expressed in Ab-Pre and Ab-Ad groups was conducted to explore the effect of miRNAs and mRNAs on fat deposition. We identified 80 differentially expressed miRNAs (DEMs) candidates, 58 of which were up-regulated and 22 down-regulated. Furthermore, six miRNAs and six mRNAs were verified by qRT-PCR, and the results showed that the expression of the DEMs and differentially expressed genes (DEGs) in the two groups was consistent with our sequencing results. When target genes of miRNA were combined with mRNA transcriptome data, a total of 891 intersection genes were obtained, we predicted the signal pathways of cross genes enrichment to the MAPK signal pathway, insulin signal pathway, fatty acid metabolism, and ECM–receptor interaction. Meanwhile, we constructed miRNA and negatively correlated mRNA target networks, including 12 miRNA–mRNAs pairs, which showed a strong association with the abdominal adipocyte differentiation (miR-214−ACSBG2, NFKB2, CAMK2A, ACLY, CCND3, PLK3, ITGB2; miR-148a-5p−ROCK2; miR-10a-5p−ELOVL5; miR-146b-5p−LAMA4; miR-6615-5p−FLNB; miR-1774−COL6A1). Overall, these findings provide a background for further research on lipid metabolism. Thus, we can better understand the molecular genetic mechanism of chicken abdominal fat deposition.

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Comparative analysis of the exosomal contents of DF-1 cells infected by ALV-J

Veterinární Medicína ◽

10.17221/141/2020-vetmed ◽

2021 ◽

Author(s):

J Yang ◽

P Zhuang ◽

Z Cheng ◽

G Wang

Keyword(s):

Target Genes ◽

Viral Pathogenesis ◽

Signal Pathway ◽

Differentially Expressed ◽

Receptor Interaction ◽

Signal Pathways ◽

Differentially Expressed Proteins ◽

Detection Technology ◽

Subgroup J ◽

Avian Leukosis Virus Subgroup

Exploration of the abnormal expression of exosomal molecules during the infection of avian leukosis virus subgroup J (ALV-J) is essential to provide a deeper understanding of the exosome’s role in the viral pathogenesis involved. The study aimed to investigate the differentially expressed proteins and miRNAs of the exosomes derived from DF-1 cells infected by ALV-J, their gene function and involved signal pathways. We isolated exosomes from DF-1 cells infected by ALV-J. The differentially expressed proteins and miRNAs of the exosomes were determined by proteomics and transcription detection technology. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway analysis identified the miRNAs target genes and the signal pathways regulated by the different proteins or/and miRNAs. A total of 116 proteins (58 upregulated and 58 downregulated) and 3 miRNAs (all upregulated) were determined. These proteins were involved in 155 signal pathways, in which the highest number of proteins involved in the cancer pathway was (up to) seven. The target genes of the miRNAs were involved in 3 signal pathways. Both the proteins and target genes of the miRNAs were involved in the Ribosome pathway and ECM-receptor interaction pathway. The results suggested that the ALV-J infection changed the proteins and miRNAs of the exosomes significantly.

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Specific Microbial Taxa and Functional Capacity Contribute to Chicken Abdominal Fat Deposition

Frontiers in Microbiology ◽

10.3389/fmicb.2021.643025 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hai Xiang ◽

Jiankang Gan ◽

Daoshu Zeng ◽

Jing Li ◽

Hui Yu ◽

...

Keyword(s):

Gut Microbiota ◽

Complex Traits ◽

Strong Association ◽

16S Rrna Gene Sequencing ◽

Abdominal Fat ◽

Fat Deposition ◽

Rrna Gene ◽

Early Maturation ◽

Chicken Fat ◽

Abdominal Fat Deposition

Genetically selected chickens with better growth and early maturation show an incidental increase in abdominal fat deposition (AFD). Accumulating evidence reveals a strong association between gut microbiota and adiposity. However, studies focusing on the role of gut microbiota in chicken obesity in conventional breeds are limited. Therefore, 400 random broilers with different levels of AFD were used to investigate the gut microbial taxa related to AFD by 16S rRNA gene sequencing of 76 representative samples, and to identify the specific microbial taxa contributing to fat-related metabolism using shotgun metagenomic analyses of eight high and low AFD chickens. The results demonstrated that the richness and diversity of the gut microbiota decrease as the accumulation of chicken abdominal fat increases. The decrease of Bacteroidetes and the increase of Firmicutes were correlated with the accumulation of chicken AFD. The Bacteroidetes phylum, including the genera Bacteroides, Parabacteroides, and the species, B. salanitronis, B. fragilis, and P. distasonis, were correlated to alleviate obesity by producing secondary metabolites. Several genera of Firmicutes phylum with circulating lipoprotein lipase activity were linked to the accumulation of chicken body fat. Moreover, the genera, Olsenella and Slackia, might positively contribute to fat and energy metabolism, whereas the genus, Methanobrevibacter, was possible to enhance energy capture, and associated to accumulate chicken AFD. These findings provide insights into the roles of the gut microbiota in complex traits and contribute to the development of effective therapies for the reduction of chicken fat accumulation.

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Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis

International Journal of Molecular Sciences ◽

10.3390/ijms20122923 ◽

2019 ◽

Vol 20 (12) ◽

pp. 2923 ◽

Cited By ~ 3

Author(s):

Jiahui Chen ◽

Xueyi Ren ◽

Limin Li ◽

Shiyi Lu ◽

Tian Chen ◽

...

Keyword(s):

Cell Line ◽

Binding Protein ◽

Fat Metabolism ◽

Ectopic Expression ◽

Abdominal Fat ◽

Fat Deposition ◽

Receptor Interaction ◽

Peroxisome Proliferator ◽

Native Chicken ◽

Abdominal Fat Deposition

Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.

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Proinflammatory cytokines and cardiac abnormalities in uncomplicated obesity: Relationship with abdominal fat deposition

Nutrition Metabolism and Cardiovascular Diseases ◽

10.1016/j.numecd.2006.01.001 ◽

2007 ◽

Vol 17 (4) ◽

pp. 294-302 ◽

Cited By ~ 67

Author(s):

Alexis E. Malavazos ◽

Massimiliano M. Corsi ◽

Federica Ermetici ◽

Calin Coman ◽

Francesco Sardanelli ◽

...

Keyword(s):

Proinflammatory Cytokines ◽

Abdominal Fat ◽

Fat Deposition ◽

Cardiac Abnormalities ◽

Abdominal Fat Deposition

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Error in Acknowledgments in: Selective Protection Against Extremes in Childhood Body Size, Abdominal Fat Deposition, and Fat Patterning in Breastfed Children

Archives of Pediatrics and Adolescent Medicine ◽

10.1001/archpediatrics.2012.1232 ◽

2012 ◽

Vol 166 (7) ◽

pp. 607

Keyword(s):

Body Size ◽

Abdominal Fat ◽

Fat Deposition ◽

Selective Protection ◽

Abdominal Fat Deposition

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RNA-Seq Analysis Reveals Hub Genes Involved in Chicken Intramuscular Fat and Abdominal Fat Deposition During Development

Frontiers in Genetics ◽

10.3389/fgene.2020.01009 ◽

2020 ◽

Vol 11 ◽

Author(s):

Siyuan Xing ◽

Ranran Liu ◽

Guiping Zhao ◽

Lu Liu ◽

Martien A. M. Groenen ◽

...

Keyword(s):

Intramuscular Fat ◽

Abdominal Fat ◽

Fat Deposition ◽

Rna Seq ◽

Hub Genes ◽

Abdominal Fat Deposition

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Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model

Frontiers in Genetics ◽

10.3389/fgene.2018.00262 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 9

Author(s):

Bahareldin A. Abdalla ◽

Jie Chen ◽

Qinghua Nie ◽

Xiquan Zhang

Keyword(s):

Abdominal Fat ◽

Fat Deposition ◽

Multiple Factors ◽

Abdominal Fat Deposition ◽

Chicken Model

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Dietary habits and physical activity: Which influence on abdominal fat deposition in children and adolescents?

Mediterranean Journal of Nutrition and Metabolism ◽

10.3233/mnm-200407 ◽

2020 ◽

Vol 13 (3) ◽

pp. 215-223

Author(s):

Rachele De Giuseppe ◽

Valeria Calcaterra ◽

Ginevra Biino ◽

Noelia Rodriguez ◽

Anna Gerbaldo ◽

...

Keyword(s):

Physical Activity ◽

Abdominal Obesity ◽

Dietary Habits ◽

Cross Sectional Study ◽

Abdominal Fat ◽

Normal Weight ◽

Fat Deposition ◽

Cross Sectional ◽

Validated Questionnaire ◽

Abdominal Fat Deposition

BACKGROUND: Prevention of childhood obesity is recommended throughout interventions aiming at enhancing healthy dietary habits (DH) and increasing physical activity (PA). OBJECTIVE: This cross-sectional study aimed at investigating DH and PA, in a sample of Italian children/adolescents. METHODS: We evaluated DH and PA by means of a previously validated questionnaire in 178 children/adolescents (88F/90M, 11.8±2.6 years): 49 normal weight (NW), 76 overweight (OW) and 53 with obesity (OB). Abdominal obesity was defined as Waist to Height Ratio (WHeR) >0.5. RESULTS: DH did not differ in NW subjects compared to OW and OB but higher PA score (p = 0.001) was observed in NW than OW and OB. No association was found between DH and WHeR, unlike PA. Four clusters of subjects were identified that could be defined by DH and PA; only one cluster, defined by PA, showed a significantly lower WHeR within subjects playing sports at least 3–4 hours/week. Moreover, subjects belonging to this cluster did not skip the main meals, eat a varied diet and drank milk at breakfast. CONCLUSIONS: In our sample PA performed at least 3–4 hours/week is inversely associated with abdominal fat deposition and is expected to positively impact health, protecting from abdominal obesity.

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Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness

Animals ◽

10.3390/ani10040624 ◽

2020 ◽

Vol 10 (4) ◽

pp. 624

Author(s):

Kai Xing ◽

Xitong Zhao ◽

Yibing Liu ◽

Fengxia Zhang ◽

Zhen Tan ◽

...

Keyword(s):

Target Genes ◽

Expression Patterns ◽

Fat Deposition ◽

Functional Enrichment ◽

Differentially Expressed ◽

Backfat Thickness ◽

P Value ◽

Sibling Pairs ◽

Differentially Expressed Mirnas

Fatty traits are very important in pig production. However, the role of microRNAs (miRNAs) in fat deposition is not clearly understood. In this study, we compared adipose miRNAs from three full-sibling pairs of female Landrace pigs, with high and low backfat thickness, to investigate the associated regulatory network. We obtained an average of 17.29 million raw reads from six libraries, 62.27% of which mapped to the pig reference genome. A total of 318 pig miRNAs were detected among the samples. Among them, 18 miRNAs were differentially expressed (p-value < 0.05, |log2fold change| ≥ 1) between the high and low backfat groups; 6 were up-regulated and 12 were down-regulated. Functional enrichment of the predicted target genes of the differentially expressed miRNAs, indicated that these miRNAs were involved mainly in lipid and carbohydrate metabolism, and glycan biosynthesis and metabolism. Comprehensive analysis of the mRNA and miRNA transcriptomes revealed possible regulatory relationships for fat deposition. Negatively correlated mRNA–miRNA pairs included miR-137–PPARGC1A, miR-141–FASN, and miR-122-5p–PKM, indicating these interactions may be key regulators of fat deposition. Our findings provide important insights into miRNA expression patterns in the backfat tissue of pig and new insights into the regulatory mechanisms of fat deposition in pig.

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