scholarly journals Caffeic Acid Phenethyl Ester Inhibits UV-Induced MMP-1 Expression by Targeting Histone Acetyltransferases in Human Skin

2019 ◽  
Vol 20 (12) ◽  
pp. 3055 ◽  
Author(s):  
Eun Ju Shin ◽  
Seongin Jo ◽  
Hyo-kyoung Choi ◽  
Sungbin Choi ◽  
Sanguine Byun ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Human Skin ◽  
Ex Vivo ◽  
Bioactive Compound ◽  
Caffeic Acid Phenethyl Ester ◽  
Inhibitory Effect ◽  
Dermal Fibroblasts ◽  
Regulatory Pathways ◽  
Skin Photoaging ◽  
Hdf Cells

Caffeic acid phenethyl ester (CAPE), a naturally occurring bioactive compound, displays anti-inflammatory, anti-carcinogenic, and anti-microbial effects. However, the effect of CAPE on skin photoaging is unknown. Herein, we investigated the inhibitory effect of CAPE against ultraviolet (UV) irradiation-mediated matrix metalloproteinase (MMP)-1 expression and its underlying molecular mechanism. CAPE treatment suppressed UV-induced MMP-1 levels in both human dermal fibroblasts (HDF) and human skin tissues. While CAPE did not display any significant effects against the upstream regulatory pathways of MMP-1, CAPE was capable of reversing UV-mediated epigenetic modifications. CAPE suppressed UV-induced acetyl-histone H3 (Lys9) as well as total lysine acetylation in HDF cells. Similarly, CAPE also attenuated UV-induced lysine acetylations in human skin tissues, suggesting that the CAPE-mediated epigenetic alterations can be recapitulated in ex vivo conditions. CAPE was found to attenuate UV-induced histone acetyltransferase (HAT) activity in HDF. Notably, CAPE was able to directly inhibit the activity of several HATs including p300, CREP-binding protein (CBP), and p300/CBP-associated factor (PCAF), further confirming that CAPE can function as an epigenetic modulator. Thus, our study suggests that CAPE maybe a promising agent for the prevention of skin photoaging via targeting HATs.

Inhibitory effect of caffeic acid phenethyl ester (CAPE) on LPS-induced inflammation of human middle ear epithelial cells

2008 ◽  
Vol 128 (12) ◽  
pp. 1303-1307 ◽  
Author(s):  
Jae-Jun Song ◽  
Jae Gu Cho ◽  
Soon-Jae Hwang ◽  
Chang Gun Cho ◽  
Seok-Won Park ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Caffeic Acid ◽  
Middle Ear ◽  
Caffeic Acid Phenethyl Ester ◽  
Inhibitory Effect ◽  
Human Middle Ear

Myrcene, an Aromatic Volatile Compound, Ameliorates Human Skin Extrinsic Aging via Regulation of MMPs Production

2017 ◽  
Vol 45 (05) ◽  
pp. 1113-1124 ◽  
Author(s):  
Eunson Hwang ◽  
Hien T. T. Ngo ◽  
Bom Park ◽  
Seul-A Seo ◽  
Jung-Eun Yang ◽  
...  
Keyword(s):  
Human Skin ◽  
Volatile Compound ◽  
Food Industry ◽  
Transforming Growth Factor ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Protective Effects ◽  
Type I Procollagen ◽  
Matrix Metalloproteinase 1 ◽  
Skin Photoaging

Myrcene is an aromatic volatile compound that is commercially well-known as a flavor ingredient in the food industry and a fragrance in the soap and detergent industry. Given the worldwide interest in natural antiphotoaging products, we investigated the protective effects of myrcene in UVB-irradiated human dermal fibroblasts (NHDFs). NHDFs were subjected to 144[Formula: see text]mJ/cm2of UVB irradiation. The expression of intracellular reactive oxygen species (ROS), matrix metalloproteinase-1 (MMP-1), MMP-3, interleukin-6 (IL-6), transforming growth factor (TGF-[Formula: see text]1) and type I procollagen were examined. We showed that myrcene decreased the production of ROS, MMP-1, MMP-3, and IL-6, and increased TGF-[Formula: see text]1 and type I procollagen secretions. Furthermore, myrcene treatment (0.1–10[Formula: see text][Formula: see text]M) dramatically reduced the activation of MAPK-related signaling molecules such as p-ERK, p-p38, and p-JNK and AP-1 including p-c-Jun and p-c-Fos. Our data indicate that myrcene has a potential protective effect on UVB-induced human skin photoaging. Therefore, myrcene might have applications in the skincare industry.

In Vitro and in Vivo Stability of Caffeic Acid Phenethyl Ester, a Bioactive Compound of Propolis

2007 ◽  
Vol 55 (9) ◽  
pp. 3398-3407 ◽  
Author(s):  
Nicola Celli ◽  
Luana K. Dragani ◽  
Stefania Murzilli ◽  
Tommaso Pagliani ◽  
Andreina Poggi
Keyword(s):  
Caffeic Acid ◽  
Bioactive Compound ◽  
Caffeic Acid Phenethyl Ester

The inhibitory effect of propolis and caffeic acid phenethyl ester on cyclooxygenase activity in J774 macrophages

Phytomedicine ◽  
2002 ◽  
Vol 9 (6) ◽  
pp. 530-535 ◽  
Author(s):  
A. Rossi ◽  
A. Ligresti ◽  
R. Longo ◽  
A. Russo ◽  
F. Borrelli ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Inhibitory Effect ◽  
Cyclooxygenase Activity

Inhibitory Effect of Caffeic Acid Phenethyl Ester on Angiogenesis, Tumor Invasion, and Metastasis

2003 ◽  
Vol 51 (27) ◽  
pp. 7907-7912 ◽  
Author(s):  
Hui-Fen Liao ◽  
Yu-Ywan Chen ◽  
Jun-Jen Liu ◽  
Ming-Ling Hsu ◽  
Hui-Ju Shieh ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Tumor Invasion ◽  
Caffeic Acid Phenethyl Ester ◽  
Inhibitory Effect ◽  
Invasion And Metastasis

scholarly journals Elucidating the Cellular Mechanism for E2-induced Dermal Fibrosis

2021 ◽  
Author(s):  
DeAnna Baker Frost ◽  
Alisa Savchenko ◽  
Adeyemi Ogunleye ◽  
Milton Armstrong ◽  
Carol Feghali-Bostwick
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
Mapk Pathway ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Human Dermal Fibroblasts ◽  
Ici 182,780 ◽  
Dermal Fibrosis ◽  
Human Skin Tissue

Abstract Background: Both TGFb and estradiol (E2), a form of estrogen, are pro-fibrotic in the skin. In the connective tissue disease, systemic sclerosis (SSc), both TGFb and E2 are likely pathogenic. Yet, the regulation of TGFb in E2-induced dermal fibrosis remains ill-defined. Elucidating those regulatory mechanisms will improve the understanding of fibrotic disease pathogenesis and set the stage for developing potential therapeutics. Using E2-stimulated primary human dermal fibroblasts in vitro and human skin tissue ex vivo, we identified the important regulatory proteins for TGFb and investigated the extracellular matrix (ECM) components that are directly stimulated by E2-induced TGFb signaling.Methods: We used primary human dermal fibroblasts in vitro and human skin tissue ex vivo stimulated with E2 or vehicle (ethanol) to measure TGFb1, TGFb2 levels using quantitative PCR (qPCR). To identify the necessary cell signaling proteins in E2-induced TGFb1 and TGFb2 transcription, human dermal fibroblasts were pre-treated with an inhibitor of the extracellular signal-regulated kinase/ mitogen-activated protein kinase (ERK/MAPK) pathway, U0126. Finally, human skin tissue ex vivo was pre-treated with SB-431542, a TGFb receptor inhibitor, and ICI 182,780, an estrogen receptor α (ER α) inhibitor, to establish the effects of TGFb and ER α signaling on E2-induced collagen 22A1 (Col22A1) transcription. Results: We found that expression of TGFb1, TGFb2, and Col22A1, a TGFb-responsive gene, are induced in response to E2 stimulation. Mechanistically, Col22A1 induction was blocked by SB-431542 and ICI 182,780 despite E2 stimulation. Additionally, inhibiting E2-induced ERK/MAPK activation and early growth response 1 (EGR1) transcription prevents the E2-induced increase in TGFb1 and TGFb2 transcription and translation. Conclusions: We conclude that E2-induced dermal fibrosis occurs in part through induction of TGFb1, 2 and Col22A1, which is regulated through EGR1 and the MAPK pathway. Thus, blocking estrogen signaling and/or production may be a novel therapeutic option in pro-fibrotic diseases.

Inhibitory effect of caffeic acid phenethyl ester on bleomycine-induced lung fibrosis in rats

2004 ◽  
Vol 339 (1-2) ◽  
pp. 65-75 ◽  
Author(s):  
Hüseyin Özyurt ◽  
Sadık Söğüt ◽  
Zeki Yıldırım ◽  
Levent Kart ◽  
Mustafa Iraz ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Lung Fibrosis ◽  
Caffeic Acid Phenethyl Ester ◽  
Inhibitory Effect
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