scholarly journals Heat-Responsive Proteomics of a Heat-Sensitive Spinach Variety

2019 ◽  
Vol 20 (16) ◽  
pp. 3872 ◽  
Author(s):  
Shanshan Li ◽  
Juanjuan Yu ◽  
Ying Li ◽  
Heng Zhang ◽  
Xuesong Bao ◽  
...  
Keyword(s):  
Heat Stress ◽  
Molecular Mechanisms ◽  
Spinacia Oleracea ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Absolute Quantification ◽  
Ros Scavenging ◽  
Two Dimensional Gel Electrophoresis ◽  
Conservative Strategy ◽  
Heat Tolerant

High temperatures seriously limit plant growth and productivity. Investigating heat-responsive molecular mechanisms is important for breeding heat-tolerant crops. In this study, heat-responsive mechanisms in leaves from a heat-sensitive spinach (Spinacia oleracea L.) variety Sp73 were investigated using two-dimensional gel electrophoresis (2DE)-based and isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics approaches. In total, 257 heat-responsive proteins were identified in the spinach leaves. The abundance patterns of these proteins indicated that the photosynthesis process was inhibited, reactive oxygen species (ROS) scavenging pathways were initiated, and protein synthesis and turnover, carbohydrate and amino acid metabolism were promoted in the spinach Sp73 in response to high temperature. By comparing this with our previous results in the heat-tolerant spinach variety Sp75, we found that heat inhibited photosynthesis, as well as heat-enhanced ROS scavenging, stress defense pathways, carbohydrate and energy metabolism, and protein folding and turnover constituting a conservative strategy for spinach in response to heat stress. However, the heat-decreased biosynthesis of chlorophyll and carotenoid as well as soluble sugar content in the variety Sp73 was quite different from that in the variety Sp75, leading to a lower capability for photosynthetic adaptation and osmotic homeostasis in Sp73 under heat stress. Moreover, the heat-reduced activities of SOD and other heat-activated antioxidant enzymes in the heat-sensitive variety Sp73 were also different from the heat-tolerant variety Sp75, implying that the ROS scavenging strategy is critical for heat tolerance.

scholarly journals Influence of heat stress on leaf morphology and nitrogen–carbohydrate metabolisms in two wucai (Brassica campestris L.) genotypes

2017 ◽  
Vol 86 (2) ◽  
Author(s):  
Lingyun Yuan ◽  
Ling Tang ◽  
Shidong Zhu ◽  
Jinfeng Hou ◽  
Guohu Chen ◽  
...  
Keyword(s):  
Heat Stress ◽  
Plant Growth ◽  
Leaf Morphology ◽  
Nitrogen Assimilation ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Physiological Mechanism ◽  
Growth And Yield ◽  
Metabolic Enzyme ◽  
Heat Tolerant

Heat stress is a major environmental stress that limits plant growth and yield worldwide. The present study was carried out to explore the physiological mechanism of heat tolerant to provide the theoretical basis for heat-tolerant breeding. The changes of leaf morphology, anatomy, nitrogen assimilation, and carbohydrate metabolism in two wucai genotypes (WS-1, heat tolerant; WS-6, heat sensitive) grown under heat stress (40°C/30°C) for 7 days were investigated. Our results showed that heat stress hampered the plant growth and biomass accumulation in certain extent in WS-1 and WS-6. However, the inhibition extent of WS-1 was significantly smaller than WS-6. Thickness of leaf lamina, upper epidermis, and palisade mesophyll were increased by heat in WS-1, which might be contributed to the higher assimilation of photosynthates. During nitrogen assimilation, WS-1 possessed the higher nitrogen-related metabolic enzyme activities, including nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH), which were reflected by higher photosynthetic nitrogen-use efficiency (PNUE) with respect to WS-6. The total amino acids level had no influence in WS-1, whereas it was reduced in WS-6 by heat. And the proline contents of both wucai genotypes were all increased to respond the heat stress. Additionally, among all treatments, the total soluble sugar content of WS-1 by heat got the highest level, including higher contents of sucrose, fructose, and starch than those of WS-6. Moreover, the metabolism efficiency of sucrose to starch in WS-1 was greater than WS-6 under heat stress, proved by higher activities of sucrose phosphate synthase (SPS), sucrose synthase (SuSy), acid invertase (AI), and amylase. These results demonstrated that leaf anatomical alterations resulted in higher nitrogen and carbon assimilation in heat-tolerant genotype WS-1, which exhibited a greater performance to resist heat stress.

scholarly journals Antioxidant Responses and Gene Expression in Bermudagrass under Cold Stress

2014 ◽  
Vol 139 (6) ◽  
pp. 699-705 ◽  
Author(s):  
Jibiao Fan ◽  
Jing Ren ◽  
Weixi Zhu ◽  
Erick Amombo ◽  
Jinmin Fu ◽  
...  
Keyword(s):  
Cold Stress ◽  
Molecular Mechanisms ◽  
Cynodon Dactylon ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Molecular Alteration ◽  
Antioxidant Genes ◽  
Dramatic Decline ◽  
Malondialdehyde Content ◽  
Key Factor

Cold stress is a key factor limiting resource use in bermudagrass (Cynodon dactylon). Under cold stress, bermudagrass growth is severely inhibited and the leaves undergo chlorosis. Therefore, rigorous investigation on the physiological and molecular mechanisms of cold stress in this turf species is urgent. The objective of this study was to investigate the physiological and molecular alteration in wild bermudagrass under cold stress, particularly the changes of transpiration rate, soluble sugar content, enzyme activities, and expression of antioxidant genes. Wild bermudagrass (C. dactylon) was planted in plastic pots (each 10 cm tall and 8 cm in diameter) filled with matrix (brown coal soil:sand 1:1) and treated with 4 °C in a growth chamber. The results displayed a dramatic decline in the growth and transpiration rates of the wild bermudagrass under 4 °C temperature. Simultaneously, cold severely destabilized the cell membrane as indicated by increased malondialdehyde content and electrolyte leakage value. Superoxide dismutase and peroxidase activities were higher in the cold regime than the control. The expression of antioxidant genes including MnSOD, Cu/ZnSOD, POD, and APX was vividly up-regulated after cold stress. In summary, our results contributed to the understanding of the role of the antioxidant system in bermudagrass’ response to cold.

scholarly journals Effect of Heat Stress on Growth and Physiological Traits of Alfalfa (Medicago sativa L.) and a Comprehensive Evaluation for Heat Tolerance

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 597 ◽  
Author(s):  
Misganaw Wassie ◽  
Weihong Zhang ◽  
Qiang Zhang ◽  
Kang Ji ◽  
Liang Chen
Keyword(s):  
Heat Stress ◽  
Medicago Sativa ◽  
Membership Function ◽  
Heat Tolerance ◽  
Comprehensive Evaluation ◽  
Soluble Sugar ◽  
Fluorescence Analysis ◽  
Relative Water ◽  
Medicago Sativa L ◽  
Heat Tolerant

Alfalfa (Medicago sativa L.) is a valuable forage legume, but its production is largely affected by high temperature. In this study, we investigated the effect of heat stress on 15 alfalfa cultivars to identify heat-tolerant and -sensitive cultivars. Seedlings were exposed to 38/35 °C day/night temperature for 7 days and various parameters were measured. Heat stress significantly reduced the biomass, relative water content (RWC), chlorophyll content, and increased the electrolyte leakage (EL) and malondialdehyde (MDA) content of heat-sensitive alfalfa cultivars. However, heat-tolerant cultivars showed higher soluble sugar (SS) and soluble protein (SP) content. The heat tolerance of each cultivar was comprehensively evaluated based on membership function value. Cultivars with higher mean membership function value of 0.86 (Bara310SC) and 0.80 (Magna995) were heat tolerant, and Gibraltar and WL712 with lower membership function value (0.24) were heat sensitive. The heat tolerance of the above four cultivars were further evaluated by chlorophyll a fluorescence analysis. Heat stress significantly affected the photosynthetic activity of heat-sensitive cultivars. The overall results indicate that Bara310SC and WL712 are heat-tolerant and heat-sensitive cultivars, respectively. This study provides basic information for understanding the effect of heat stress on growth and productivity of alfalfa.

scholarly journals A Long-Day Photoperiod and 6-Benzyladenine Promote Runner Formation through Upregulation of Soluble Sugar Content in Strawberry

2020 ◽  
Vol 21 (14) ◽  
pp. 4917
Author(s):  
Yali Li ◽  
Jiangtao Hu ◽  
Hao Wei ◽  
Byoung Ryong Jeong
Keyword(s):  
Molecular Mechanisms ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Orthogonal Test ◽  
Influential Factor ◽  
Asexual Propagation ◽  
Soluble Sugar Content ◽  
Long Day ◽  
Seed Propagation ◽  
Dimensional Electrophoresis

Commercial strawberries are mainly propagated using daughter plants produced on aerial runners because asexual propagation is faster than seed propagation, and daughter plants retain the characteristics of the mother plant. This study was conducted to investigate the effective factors for runner induction, as well as the molecular mechanisms behind the runner induction. An orthogonal test with 4 factors (photoperiod, temperature, gibberellin, and 6-benzyladenine), each with 3 levels was performed. Proteins were also extracted from the crowns with or without runners and separated by two-dimensional electrophoresis. The results of the orthogonal test showed that a long-day (LD) environment was the most influential factor for the runner formation, and 50 mg·L−1 of 6-BA significantly increased the number of runners. A proteomic analysis revealed that 32 proteins were differentially expressed (2-fold, p < 0.05) in the strawberry crowns with and without runners. A total of 16 spots were up-regulated in the crowns with runners induced by LD treatment. Identified proteins were classified into seven groups according to their biological roles. The most prominent groups were carbohydrate metabolism and photosynthesis, which indicated that the carbohydrate content may increase during runner formation. A further analysis demonstrated that the soluble sugar content was positively correlated with the number of runners. Thus, it is suggested that the photoperiod and 6-BA break the dormancy of the axillary buds and produce runners by increasing the soluble sugar content in strawberry.

scholarly journals WHIRLY1 Regulates HSP21.5A Expression to Promote Thermotolerance in Tomato

2019 ◽  
Vol 61 (1) ◽  
pp. 169-177 ◽  
Author(s):  
Kunyang Zhuang ◽  
Yangyang Gao ◽  
Zhuangbin Liu ◽  
Pengfei Diao ◽  
Na Sui ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Crop Yields ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Membrane Stability ◽  
Heat Shock Factors ◽  
Soluble Sugar Content ◽  
Ros Accumulation ◽  
Elicitor Response

Abstract Heat stress poses a major threat to plant productivity and crop yields. The induction of heat shock proteins (HSPs) by heat shock factors is a principal defense response of plants exposed to heat stress. In this study, we identified and analyzed the heat stress-induced Whirly1 (SlWHY1) gene in tomato (Solanum lycopersicum). We generated various SlWHY1-overexpressing (OE) and SlWHY1-RNA interference (RNAi) lines to investigate the role of WHIRLY1 in thermotolerance. Compared with the wild type (WT), the OE lines showed less wilting, as reflected by their increased membrane stability and soluble sugar content and reduced reactive oxygen species (ROS) accumulation under heat stress. By contrast, RNAi lines with inhibited SlWHY1 expression showed the opposite phenotype and corresponding physiological indices under heat stress. The heat-induced gene SlHSP21.5A, encoding an endoplasmic reticulum-localized HSP, was upregulated in the OE lines and downregulated in the RNAi lines compared with the WT. RNAi-mediated inhibition of SlHSP21.5A expression also resulted in reduced membrane stability and soluble sugar content and increased ROS accumulation under heat stress compared with the WT. SlWHY1 binds to the elicitor response element-like element in the promoter of SlHSP21.5A to activate its transcription. These findings suggest that SlWHY1 promotes thermotolerance in tomato by regulating SlHSP21.5A expression.

scholarly journals Downregulation of PyHRG1, encoding a novel secretory protein in the red alga Pyropia yezoensis, enhances heat tolerance

ALGAE ◽  
2021 ◽  
Vol 36 (3) ◽  
pp. 207-217
Author(s):  
Narae Han ◽  
Jiwoong Wi ◽  
Sungoh Im ◽  
Ka-Min Lim ◽  
Hun-Dong Lee ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Tolerance ◽  
Red Algae ◽  
Molecular Mechanisms ◽  
Seawater Temperature ◽  
Secretory Protein ◽  
Red Alga ◽  
Pyropia Yezoensis ◽  
Tolerant Mutant ◽  
Heat Tolerant

An increase in seawater temperature owing to global warming is expected to substantially limit the growth of marine algae, including Pyropia yezoensis, a commercially valuable red alga. To improve our knowledge of the genes involved in the acquisition of heat tolerance in P. yezoensis, transcriptomes sequences were obtained from both the wild-type SG104 P. yezoensis and heat-tolerant mutant Gy500. We selected 1,251 differentially expressed genes that were up- or downregulated in response to the heat stress condition and in the heat-tolerant mutant Gy500, based on fragment per million reads expression values. Among them, PyHRG1 was downregulated under heat stress in SG104 and expressed at a low level in Gy500. PyHRG1 encodes a secretory protein of 26.5 kDa. PyHRG1 shows no significant sequence homology with any known genes deposited in public databases to date. However, PyHRG1 homologs were found in other red algae, including other Pyropia species. When PyHRG1 was introduced into the single-cell green alga Chlamydomonas reinhardtii, transformed cells overexpressing PyHRG1 showed severely retarded growth. These results demonstrate that PyHRG1 encodes a novel red algae-specific protein and plays a role in heat tolerance in algae. The transcriptome sequences obtained in this study, which include PyHRG1, will facilitate future studies to understand the molecular mechanisms involved in heat tolerance in red algae.

scholarly journals Comparative Transcriptomic Analysis of Two Cucumis Melo Var. Saccharinus Germplasms Differing in Fruit Physical and Chemical Characteristics

2021 ◽  
Author(s):  
Renfan Liang ◽  
Yicheng Su ◽  
Xiaojuan Qin ◽  
Zhongkui Gao ◽  
Zhixin Fu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fruit Development ◽  
Cucumis Melo ◽  
Molecular Mechanisms ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Chemical Characteristics ◽  
Physical And Chemical Characteristics ◽  
Cell Wall Biogenesis ◽  
Physical And Chemical

Abstract Background: Hami melon (Cucumis melo var. saccharinus) is a popular fruit in China noted for its excellent taste, which is largely determined by its physical and chemical characteristics, including flesh texture, sugar content, aroma, and nutrient composition. However, the mechanisms through which the associated with these characteristics are regulated have not yet been sufficiently determined. In this study, we monitored changes in the fruits of two germplasms differing in physical and chemical characteristics throughout the period of fruit development. Results: Ripe fruit of the bred variety ‘Guimi’ had significantly higher soluble sugar contents than the fruit of the common variety ‘Yaolong’, whereas differences in fruit shape and color between these two germplasms were observed during the course of development. Comparative transcriptome analysis, conducted to identify regulators and pathways underlying the observed differences at corresponding stages of development, revealed a higher number of differentially expressed genes (DEGs) in Guimi than in Yaolong. Moreover, most of the DEGs detected during early fruit development of Guimi were associated with cell wall biogenesis. Temporal analysis of the identified DEGs revealed similar trends in the enrichment of downregulated genes in both germplasms, although there were certain differences in the enrichment trends of upregulated genes. Further analyses revealed trends in the differential changes of multiple genes involved in cell wall biogenesis and sugar metabolism during fruit ripening.Conclusions: We were thus able to identify a number of genes associated with the ripening of Hami melon, which will accordingly provide novel insights into the molecular mechanisms underlying the development of fruit characteristics in these melons.

scholarly journals Cytosine methylations in the promoter regions of genes involved in the cellular oxidation equilibrium pathways affect rice heat tolerance

2020 ◽  
Author(s):  
Chao He ◽  
Hong-Yu Zhang ◽  
Yong-Xin Zhang ◽  
Pei Fu ◽  
Li-Li You ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Heat Tolerance ◽  
Molecular Mechanisms ◽  
Pentatricopeptide Repeat ◽  
Ros Scavenging ◽  
Promoter Regions ◽  
Tolerant Strain ◽  
Rice Strain ◽  
Heat Tolerant ◽  
Methylation Patterns

Abstract Background: High temperatures, particularly at night, decrease rice yield and quality. As high nighttime temperatures (HNTs) become increasingly frequent due to climate change, it is imperative to develop rice crops that tolerate HNTs. DNA methylation may represent a potential avenue for HNT-tolerant rice strain development, as this mechanism regulates gene activity and cellular phenotype in response to adverse environmental conditions without changing the nucleotide sequence.Results: After HNT exposure, the methylation patterns of cytosines in the CHH context differed noticeably between two coisogenic rice strains with significantly different levels in heat tolerance. Methylation differences between strains were primarily observed on successive cytosines in the promoter or downstream regions of transcription factors and transposon elements. In contrast to the heat-sensitive rice strain, the regions 358–359 bp and 2–60 bp downstream of two basal transcriptional factors (TFIID subunit 11 and mediator of RNA polymerase II transcription subunit 31, respectively) were fully demethylated in the heat-tolerant strain after HNT exposure. In the heat-tolerant strain, HNTs reversed the methylation patterns of successive cytosines in the promoter regions of various genes involved in abscisic acid (ABA)-related reactive oxygen species (ROS) equilibrium pathways, including the pentatricopeptide repeat domain gene PPR (LOC_Os07g28900) and the homeobox domain gene homeobox (LOC_Os01g19694). Indeed, PRR expression was inhibited in heat-sensitive rice strains, and the methylation rates of the cytosines in the promoter region of PRR were greater in heat-sensitive strains as compared to heat-tolerant strains.Conclusions: After HNT exposure, cytosines in the CHH context were more likely than cytosines in other contexts to be methylated differently between the heat-sensitive and heat-tolerant rice strains. Methylation in the promoter regions of the genes associated with ABA-related oxidation and ROS scavenging improved heat tolerance in rice. Our results help to clarify the molecular mechanisms underlying rice heat tolerance.

scholarly journals Effect of heat stress on the MDA, proline and soluble sugar content in leaf lettuce seedlings

2013 ◽  
Vol 04 (05) ◽  
pp. 112-115 ◽  
Author(s):  
Yingyan Han ◽  
Shuangxi Fan ◽  
Qiao Zhang ◽  
Yanan Wang
Keyword(s):  
Heat Stress ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugar Content ◽  
Leaf Lettuce

Small heat shock protein responses in leaf tissues of wheat cultivars with different heat susceptibility

Biologia ◽  
2008 ◽  
Vol 63 (4) ◽  
Author(s):  
Mustafa Yildiz ◽  
Hakan Terzi
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Small Heat Shock Protein ◽  
Special Importance ◽  
Wheat Cultivars ◽  
Two Dimensional Gel Electrophoresis ◽  
Leaf Tissues ◽  
Heat Tolerant ◽  
C 50

AbstractThe effect of heat stress on soluble proteins extracted from leaf tissues of bread (Triticum aestivum cv. Gönen-98, tolerant; cv. Cumhuriyet-75, susceptible; genome ABD) and durum (Triticum durum cv. Ege-88, tolerant; cv. Ankara-98, susceptible; genome AB) wheat cultivars differing in sensitivity to high temperature was examined by two-dimensional gel electrophoresis. At acclimation (37°C) and acclimation→high temperature (37°C→50°C) treatments compared to control (25°C), evaluation of gels revealed 31 proteins to be differentially expressed in first leaves as a result of heat stress in heat-susceptible and heat-tolerant cultivars of bread and durum wheats. All of the increased or decreased proteins in amount, newly synthesized and/or disappeared were in low-molecular-weight (LMW, 16.1–24.0 kDa) and generally acidic character (pI 4.8–6.9). The responses of the four cultivars were compared: Twenty-two of 31 proteins were detected as newly synthesized LMW heat shock proteins (LMW HSPs = small HSPs). The number of these sHSPs was different in cultivars which have the same genome. In addition, the number of the sHSPs in heat-tolerant cultivars was higher than in heat-susceptible cultivars. Some of the sHSPs were specific to cultivar. Most of the sHSPs synthesized at 37°C were also detected at 37°C→50°C treatment. It is suggested that sHSPs have special importance in two points: Firstly, sHSPs in cultivars showed abundance and diversity. Secondly, these proteins may play an important role in the acquiring of thermal tolerance.

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