The Role of Actin Remodelling Proteins in Wound Healing and Tissue Regeneration

SummaryNeovascularisation is crucial both for physiological processes, like development, wound healing, tissue regeneration, hair growth or menstrual cycle, and for pathological states, such as tumour progression, retinopathy and psoriasis. Blood vessel formation is orchestrated by numerous pro-angiogenic and anti-angiogenic factors, acting together to keep tight rein on this complicated, desirable but also dangerous process. One of the proteins important for neovascularisation is heme oxygenase-1 (HO-1), an enzyme degrading heme. This review focuses on the role of HO-1 in angiogenesis and vasculogenesis, having a closer look at the significance of this system in diabetes.

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Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

MRS Bulletin ◽

10.1557/mrs2010.528 ◽

2010 ◽

Vol 35 (8) ◽

pp. 597-606 ◽

Cited By ~ 39

Author(s):

Anthony Atala ◽

Darrell J. Irvine ◽

Marsha Moses ◽

Sunil Shaunak

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Regenerative Medicine ◽

Tissue Regeneration ◽

Normal Tissue ◽

Disease Process ◽

Scar Formation ◽

The Body ◽

Mechanistic Basis

AbstractOne of the major challenges in the field of regenerative medicine is how to optimize tissue regeneration in the body by therapeutically manipulating its natural ability to form scar at the time of injury or disease. It is often the balance between tissue regeneration, a process that is activated at the onset of disease, and scar formation, which develops as a result of the disease process that determines the ability of the tissue or organ to be functional. Using biomaterials as scaffolds often can provide a “bridge” for normal tissue edges to regenerate over small distances, usually up to 1 cm. Larger tissue defect gaps typically require both scaffolds and cells for normal tissue regeneration to occur without scar formation. Various strategies can help to modulate the scar response and can potentially enhance tissue regeneration. Understanding the mechanistic basis of such multivariate interactions as the scar microenvironment, the immune system, extracellular matrix, and inflammatory cytokines may enable the design of tissue engineering and wound healing strategies that directly modulate the healing response in a manner favorable to regeneration.

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Bioactive Medications for the Delivery of Platelet Derivatives to Skin Wounds

Current Drug Delivery ◽

10.2174/1381612825666190320154406 ◽

2019 ◽

Vol 16 (5) ◽

pp. 472-483 ◽

Cited By ~ 1

Author(s):

Maria Cristina Bonferoni ◽

Silvia Rossi ◽

Giuseppina Sandri ◽

Carla Caramella ◽

Claudia Del Fante ◽

...

Keyword(s):

Wound Healing ◽

Tissue Regeneration ◽

Chronic Wounds ◽

Platelet Rich Plasma ◽

Platelet Lysate ◽

Therapeutic Success ◽

External Application ◽

Wound Site ◽

Prolonged Contact

Chronic wounds are the result of alterations in the complex series of events of physiological wound healing. In particular, the prolonged inflammation results in increased protease activity, in the degradation of extracellular matrix (ECM) and of growth factors (GFs). The relevance of platelet GFs in maintaining and restoring the complex equilibrium of different moments in wound healing is well recognized. Moreover, the observed decrease of their levels in chronic wounds suggested a possible therapeutic role of the external application to the wounds. It has been also pointed out that tissue regeneration can be more efficiently obtained by the synergic use of different GFs. Platelet derivatives such as platelet- rich plasma (PRP) and platelet lysate (PL) are able to release GFs in a balanced pool. Their therapeutic use in regenerative medicine and wound healing has been therefore more and more frequently proposed in clinical trials and in the literature. The development of a suitable formulation able to control the GFs release rate, to protect the GFs, and to assure their prolonged contact with the wound site, is of paramount importance for the therapeutic success. The present review considers some formulation approaches for PRP and PL application to wounds.

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Cloning, Expression and Effects of P. americana Thymosin on Wound Healing

International Journal of Molecular Sciences ◽

10.3390/ijms20194932 ◽

2019 ◽

Vol 20 (19) ◽

pp. 4932 ◽

Cited By ~ 1

Author(s):

Jie Jing ◽

Xiaohong Sun ◽

Chuang Zhou ◽

Yifan Zhang ◽

Yongmei Shen ◽

...

Keyword(s):

Wound Healing ◽

Growth Factors ◽

Tissue Regeneration ◽

Dna Sequences ◽

Wound Repair ◽

Periplaneta Americana ◽

American Cockroach ◽

Promote Wound Healing ◽

Fibroblast Migration

The American cockroach (Periplaneta americana) is a medicinal insect. Its extract is used clinically to promote wound healing and tissue regeneration, but the effective medicinal components and mechanisms are not yet clear. It has been reported that human thymosin beta 4 (Tβ4) may accelerate skin wound healing, however, the role of P. americana thymosin (Pa-THYs) is still poorly understood. In the present study, we identify and analyze the DNA sequences of Pa-THYs by bioinformatics analysis. Then we clone, express, and purify the Pa-THYs proteins and evaluate the activity of recombinant Pa-THYs proteins by cell migration and proliferation assays in NIH/3T3 cells. To elucidate the role of Pa-THYs in wound healing, a mouse model is established, and we evaluate wound contraction, histopathological parameters, and the expressions of several key growth factors after Pa-THYs treatment. Our results showed that three THY variants were formed by skipping splicing of exons. Pa-THYs could promote fibroblast migration, but have no effect on fibroblast proliferation. In wound repair, Pa-THYs proteins could effectively promote wound healing through stimulating dermal tissue regeneration, angiogenesis, and collagen deposition. On the molecular mechanism, Pa-THYs also stimulated the expression of several key growth factors to promote wound healing. The data suggest that Pa-THYs could be a potential drug for promoting wound repair.

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Emerging role of inorganic and metal nanoparticles for the delivery of combination of drugs in wound healing and tissue regeneration

Nanocarriers for the Delivery of Combination Drugs ◽

10.1016/b978-0-12-820779-6.00012-8 ◽

2021 ◽

pp. 195-226

Author(s):

Vyshnavi Tallapaneni ◽

Divya Pamu ◽

Shilpa TN ◽

Veera Venkata Satyanarayana Reddy Karri ◽

Suresh K. Mohankumar

Keyword(s):

Wound Healing ◽

Metal Nanoparticles ◽

Tissue Regeneration

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Honey: An Effective Regenerative Medicine Product in Wound Management

Current Medicinal Chemistry ◽

10.2174/0929867325666180510141824 ◽

2019 ◽

Vol 26 (27) ◽

pp. 5230-5240 ◽

Cited By ~ 8

Author(s):

Simona Martinotti ◽

Marcela Bucekova ◽

Juraj Majtan ◽

Elia Ranzato

Keyword(s):

Wound Healing ◽

Regenerative Medicine ◽

Tissue Regeneration ◽

Potential Role ◽

Wound Management ◽

Physiological Basis ◽

Speed Up ◽

Healing Wounds ◽

Medicine Product

:Honey has successfully been used in the treatment of a broad spectrum of injuries including burns and non-healing wounds. It acts as an antibacterial and anti-biofilm agent with anti/pro-inflammatory properties. However, besides these traditional properties, recent evidence suggests that honey is also an immunomodulator in wound healing and contains several bee and plant-derived components that may speed up wound healing and tissue regeneration process. Identifying their exact mechanism of action allows better understanding of honey healing properties and promotes its wider translation into clinical practice.:This review will discuss the physiological basis for the use of honey in wound management, its current clinical uses, as well as the potential role of honey bioactive compounds in dermal regenerative medicine and tissue re-modeling.

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Anti-Angiogenetic Agents from the Sea: A New Potential Preventive and Therapeutic Wave?

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200705215226 ◽

2020 ◽

Vol 20 (17) ◽

pp. 2005-2011

Author(s):

Maria A. Gammone ◽

Antonella Danese ◽

Nicolantonio D’Orazio

Keyword(s):

Wound Healing ◽

Natural Products ◽

Tissue Regeneration ◽

Tumor Development ◽

Vascular Response ◽

Marine Plants ◽

Body Tissues ◽

Degenerative Disorders ◽

Endothelial Functions

: Angiogenesis, generation of novel blood vessels from pre-existing ones, is a prerequisite for the physiological expansion, reparation, and functioning of body tissues and systems. However, it is also involved in some pathological inflammatory situations, such as oncologic and chronic degenerative disorders. The correct angiogenesis and neo-vascular response also accompanies wound healing, interaction with biocompatible materials, and tissue regeneration. : In this respect, natural products deriving from terrestrial and marine plants/organisms may prevent and even cure various angiogenesis-dependent disorders. : Bioactive natural compounds with antioxidant and anti-inflammatory activities could concur to maintain adequate vascularization and endothelial functions and inhibit angiogenesis, thus controlling tumor development. : This review aims to illustrate the role of some marine-derived compounds as anti-angiogenetic agents.

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The growing role of eicosanoids in tissue regeneration, repair, and wound healing

Prostaglandins & Other Lipid Mediators ◽

10.1016/j.prostaglandins.2013.05.002 ◽

2013 ◽

Vol 104-105 ◽

pp. 130-138 ◽

Cited By ~ 23

Author(s):

Brian T. Kalish ◽

Mark W. Kieran ◽

Mark Puder ◽

Dipak Panigrahy

Keyword(s):

Wound Healing ◽

Tissue Regeneration

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Experience of negative pressure wound therapy over sternal wound healing: A retrospective review

WCET Journal ◽

10.33235/wcet.39.2.9-18 ◽

2019 ◽

Vol 39 (2) ◽

pp. 9-18

Author(s):

Wai Sze Ho ◽

Wai Kuen Lee ◽

Ka Kay Chan ◽

Choi Ching Fong

Keyword(s):

Wound Healing ◽

Negative Pressure ◽

Negative Pressure Wound Therapy ◽

Assessment Tool ◽

Wound Care ◽

Wound Therapy ◽

Treatment Pathways ◽

Wound Assessment ◽

One Year

Objectives The aim of this study was to retrospectively review the effectiveness of negative pressure wound therapy (NPWT) in sternal wound healing with the use of the validated Bates-Jensen Wound Assessment Tool (BWAT), and explore the role of NPWT over sternal wounds and future treatment pathways. Methods Data was gathered from patients' medical records and the institution's database clinical management system. Seventeen subjects, who had undergone cardiothoracic surgeries and subsequently consulted the wound care team in one year were reviewed. Fourteen of them were included in the analysis. Healing improvement of each sternal wound under continuous NPWT and continuous conventional dressings was studied. In total, 23 continuous NPWT and 13 conventional dressing episodes were analysed with the BWAT. Results Among conventional dressing episodes, sternal wound improvement was 2.5–3% over 10 days to 3.5 weeks, whereas 4–5% sternal healing was achieved in 5 days to 2 weeks with sternal wire presence. Better healing at 11% in 1 week by conventional dressing was attained after sternal wire removal. In NPWT episodes, 8–29%, 13–24%, and 15–46% of healing was observed in 2 weeks, 3.5 to 5 weeks and 6 to 7 weeks, respectively. Only 39% wound healing was acquired at the 13th week of NPWT in one subject. With sternal wire present, 6%–29% wound healing progress was achieved by NPWT in 1–4 weeks, and 16–23% wound improvement in 2 to 4.5 weeks by NWPT after further surgical debridement. After sternal wire removal, 6–34% sternal wound healing occurred by continuous NPWT for 1–2 weeks, and maximum healing at 46% after 2.5 weeks of NPWT were observed. Conclusions Better wound healing was achieved in the NPWT group in comparison to conventional dressings alone. However, suboptimal sternal wound healing by NPWT alone was observed. Removal of sternal wire may improve the effectiveness of NPWT. Successful tertiary closure after NPWT among subjects supports the important bridging role of NPWT in sternal wound healing. Factors causing stagnant sternal wound healing by NPWT alone are discussed.

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