Plasma Metabolic Profiling Analysis of Gout Party on Acute Gout Arthritis Rats Based on UHPLC–Q–TOF/MS Combined with Multivariate Statistical Analysis

Gout Party is a Chinese medicine prescription composed of Aconiti Lateralis Radix Praeparaia, Aconiti Radix Cocta, Cremastrae Pseudobulbus Pleiones Pseudobulbus, Smilacis Glabrae Rhizoma, Rehmanniae Radix, and Glycyrrhizae Radix et Rhizoma, which can relieve joint pain caused by gouty arthritis (GA) and rheumatoid, and has a therapeutic effect on acute gouty arthritis (AGA). However, little information is available on the molecular biological basis and therapeutic mechanism of Gout Party for the treatment of AGA. AGA model was established by injecting sodium urate, and colchicine served as a positive control drug. We established a metabolomic method based on ultra-high-performance liquid chromatography–tandem quadrupole/time-of-flight mass spectrometry (UHPLC–Q–TOF/MS) to analyze the plasma samples of model group rats and blank group rats. Multiple statistical analyses, including principal component analysis (PCA) and partial least square discrimination analysis (PLS-DA), were used to examine metabolite profile changes in plasma samples. Finally, we identified 2–ketobutyric acid, 3–hexenedioic acid, but–2–enoic acid, and so on; 22 endogenous metabolites associated with AGA. After successful molding, we found that 2–ketobutyric acid, 3–hexenedioic acid, but–2–enoic acid, argininic acid, galactonic acid, lactic acid, equol 4′–O–glucuronide, deoxycholic acid glycine conjugate, glycocholic acid, sphinganine 1–phosphate, LPE (0:0/20:3), LPE (0:0/16:0), LPC (15:0) decreased significantly (p < 0.05 or p < 0.01), alanine, erythrulose, 3–dehydrocarnitine, m–methylhippuric acid, 3–hydroxyoctanoic acid, p–cresol sulfate, estriol 3–sulfate 16–glucuronide, 10–hydroxy–9–(phosphonooxy)octadecenoate, docosahexaenoic acid increased significantly (p < 0.05 or p < 0.01). After Gout Party treatment, 14 biomarkers had a tendency to normal conditions. These above biomarkers were mainly involved in fatty acid metabolism, bile acid metabolism, amino acid metabolism, and energy metabolism pathways. These results suggested that Gout Party exerted therapeutic effects of treating AGA by improving energy metabolism disorder and amino acid metabolism dysfunction, and attenuating fatty acid metabolism abnormal and inflammation. The results of this experiment provided a reference for revealing the metabolic mechanism produced by Gout Party in the treatment of AGA, but the subsequent studies need to be further improved and supported by relevant cell experiments and clinical experiments.

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Hepatic cytochrome P-450 reductase-null mice show reduced transcriptional response to quercetin and reveal physiological homeostasis between jejunum and liver

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00565.2005 ◽

2006 ◽

Vol 291 (1) ◽

pp. G63-G72 ◽

Cited By ~ 10

Author(s):

David M. Mutch ◽

Vanessa Crespy ◽

Jennifer Clough ◽

Colin J. Henderson ◽

Sofiane Lariani ◽

...

Keyword(s):

Small Intestine ◽

Fatty Acid ◽

Amino Acid ◽

Fatty Acid Metabolism ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Glutathione Metabolism ◽

Sterol Metabolism ◽

Cytochrome P 450 ◽

Hepatic Cytochrome

Using mice deficient in hepatic cytochrome P-450 oxidoreductase (POR), which disables the liver cytochrome P-450 system, we examined the metabolism and biological response of the anticarcinogenic flavonoid, quercetin. Profiling circulating metabolites revealed similar profiles over 72 h in wild-type (WT) and POR-null (KO) mice, showing that hepatic P450 and reduced biliary secretion do not affect quercetin metabolism. Transcriptional profiling at 24 h revealed that two- to threefold more genes responded significantly to quercetin in WT compared with KO in the jejunum, ileum, colon, and liver, suggesting that hepatic P450s mediate many of the biological effects of quercetin, such as immune function, estrogen receptor signaling, and lipid, glutathione, purine, and amino acid metabolism, even though quercetin metabolism is not modified. The functional interpretation of expression data in response to quercetin (single dose of 7 mg/animal) revealed a molecular relationship between the liver and jejunum. In WT animals, amino acid and sterol metabolism was predominantly modulated in the liver, fatty acid metabolism response was shared between the liver and jejunum, and glutathione metabolism was modulated in the small intestine. In contrast, KO animals do not regulate amino acid metabolism in the liver or small intestine, they share the control of fatty acid metabolism between the liver and jejunum, and regulation of sterol metabolism is shifted from the liver to the jejunum and that of glutathione metabolism from the jejunum to the liver. This demonstrates that the quercetin-mediated regulation of these biological functions in extrahepatic tissues is dependent on the functionality of the liver POR. In conclusion, using a systems biology approach to explore the contribution of hepatic phase 1 detoxification on quercetin metabolism demonstrated the resiliency and adaptive capacity of a biological organism in dealing with a bioactive nutrient when faced with a tissue-specific molecular dysfunction.

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Starvation causes disturbance in amino acid and fatty acid metabolism in Diporeia

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2011.12.011 ◽

2012 ◽

Vol 161 (4) ◽

pp. 348-355 ◽

Cited By ~ 16

Author(s):

Suman Maity ◽

Amber Jannasch ◽

Jiri Adamec ◽

Thomas Nalepa ◽

Tomas O. Höök ◽

...

Keyword(s):

Fatty Acid ◽

Amino Acid ◽

Fatty Acid Metabolism ◽

Acid Metabolism

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Metabolome and exposome profiling of the biospecimens from COVID-19 patients in India

Journal of microbiology epidemiology immunobiology ◽

10.36233/0372-9311-161 ◽

2021 ◽

Vol 98 (4) ◽

pp. 397-415

Author(s):

Sh. Aggarwal ◽

Sh. Parihari ◽

A. Banerjee ◽

J. Roy ◽

N. Banerjee ◽

...

Keyword(s):

Amino Acid ◽

Amino Acid Metabolism ◽

Indian Population ◽

Acid Metabolism ◽

Clinical Applications ◽

Rapid Diagnosis ◽

Plasma Samples ◽

Metabolism Pathway ◽

Diagnosis And Prognosis ◽

Metabolome Profiling

Introduction. COVID-19 has become a global impediment by bringing everything to a halt starting from January 2020. India underwent the lockdown starting from 22nd March 2020 with the sudden spike in the number of COVID-19 patients in major cities and states. This study focused on how metabolites play a crucial role in SARSCoV-2 prognosis.Materials and methods. Metabolome profiling of 106 plasma samples and 24 swab samples from symptomatic patients in the Indian population of the Mumbai region was done. COVID-19 positive samples were further segregated under the non-severe COVID-19 and severe COVID-19 patient cohort for both plasma and swab.Results. After analyzing the raw files, total 7,949 and 12,871 metabolites in plasma and swab were found. 11 and 35 significantly altered metabolites were found in COVID-19 positive compared to COVID-19 negative plasma and swab samples, respectively. Also, 9 and 23 significantly altered metabolites were found in severe COVID-19 positive to non-severe COVID-19 positive plasma and swab samples, respectively. The majorly affected pathways in COVID-19 patients were found to be the amino acid metabolism pathway, sphingosine metabolism pathway, and bile salt metabolism pathway.Conclusion. This study facilitates identification of potential metabolite-based biomarker candidates for rapid diagnosis and prognosis for clinical applications.

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Lactobacillus frumenti mediates energy production via fatty acid β-oxidation in the liver of early-weaned piglets

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-019-0399-5 ◽

2019 ◽

Vol 10 (1) ◽

Author(s):

Zhichang Wang ◽

Jun Hu ◽

Wenyong Zheng ◽

Tao Yang ◽

Xinkai Wang ◽

...

Keyword(s):

Fatty Acid ◽

Amino Acid ◽

Gut Microbiota ◽

Oral Administration ◽

Energy Production ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Critical Role ◽

Guanosine Monophosphate ◽

Weaned Piglets

Abstract Background Early-weaning of piglets is often accompanied by severe disorders, especially diarrhea. The gut microbiota and its metabolites play a critical role in the maintenance of the physiologic and metabolic homeostasis of the host. Our previous studies have demonstrated that oral administration of Lactobacillus frumenti improves epithelial barrier functions and confers diarrhea resistance in early-weaned piglets. However, the metabolic response to L. frumenti administration remains unclear. Then, we conducted simultaneous serum and hepatic metabolomic analyses in early-weaned piglets administered by L. frumenti or phosphate-buffered saline (PBS). Results A total of 100 6-day-old crossbred piglets (Landrace × Yorkshire) were randomly divided into two groups and piglets received PBS (sterile, 2 mL) or L. frumenti (suspension in PBS, 108 CFU/mL, 2 mL) by oral administration once per day from 6 to 20 days of age. Piglets were weaned at 21 days of age. Serum and liver samples for metabolomic analyses were collected at 26 days of age. Principal components analysis (PCA) showed that L. frumenti altered metabolism in serum and liver. Numerous correlations (P < 0.05) were identified among the serum and liver metabolites that were affected by L. frumenti. Concentrations of guanosine monophosphate (GMP), inosine monophosphate (IMP), and uric acid were higher in serum of L. frumenti administration piglets. Pathway analysis indicated that L. frumenti regulated fatty acid and amino acid metabolism in serum and liver. Concentrations of fatty acid β-oxidation related metabolites in serum (such as 3-hydroxybutyrylcarnitine, C4-OH) and liver (such as acetylcarnitine) were increased after L. frumenti administration. Conclusions Our findings suggest that L. frumenti regulates lipid metabolism and amino acid metabolism in the liver of early-weaned piglets, where it promotes fatty acid β-oxidation and energy production. High serum concentrations of nucleotide intermediates, which may be an alternative strategy to reduce the incidence of diarrhea in early-weaned piglets, were further detected. These findings broaden our understanding of the relationships between the gut microbiota and nutrient metabolism in the early-weaned piglets.

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Effects of sodium monofluoroacetate on glucose, amino-acid, and fatty-acid metabolism and risk assessment of glucose supplementation

Drug and Chemical Toxicology ◽

10.1080/01480540903170720 ◽

2009 ◽

Vol 32 (4) ◽

pp. 353-361 ◽

Cited By ~ 3

Author(s):

Hiroshi Tsuji ◽

Hiroyasu Shimizu ◽

Tomotaro Dote ◽

Kan Usuda ◽

Masafumi Imanishi ◽

...

Keyword(s):

Risk Assessment ◽

Fatty Acid ◽

Amino Acid ◽

Fatty Acid Metabolism ◽

Acid Metabolism ◽

Glucose Supplementation

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Effects of hyperoxic gas mixtures on energy metabolism during prolonged work

Journal of Applied Physiology ◽

10.1152/jappl.1975.39.2.267 ◽

1975 ◽

Vol 39 (2) ◽

pp. 267-271 ◽

Cited By ~ 25

Author(s):

B. A. Wilson ◽

H. G. Welch ◽

J. N. Liles

Keyword(s):

Steady State ◽

Fatty Acid ◽

Energy Metabolism ◽

Fatty Acid Metabolism ◽

Acid Metabolism ◽

Co2 Storage ◽

Bicycle Ergometer ◽

Metabolic Responses ◽

Average Increase ◽

Exercise Tests

These experiments were designed to study selected respiratory and metabolic responses to exercise in hyperoxia. Four subjects were examined during 30-min bicycle ergometer rides at both 40% and 80% of their aerobic maximum. The VO2 was significantly increased at both work levels breathing 60% O2 versus 21% O2, while VCO2 showed no significant change during the 40% exercise tests but was significantly decreased during the 80% intensity rides. The average increase in the volume of O2 taken up during 30 min of hyperoxic exercise, compared with normoxia, was 3.3 liters at the 40% exercise level and 5.6 liters at the 80% level. Neither the magnitude of the O2 nor the CO2 storage calculated for the exercise bouts could explain these increases. Steady-state criteria for the gas stores were established by the stable values of PETCO2, VO2, VCO2, and VI from minute 6 through 30 at both work levels. R values decreased during the hyperoxic tests suggesting the possibility of a shift toward increased fatty acid metabolism.

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