Dietary Collagen Hydrolysates Ameliorate Furrowed and Parched Skin Caused by Photoaging in Hairless Mice

Collagen hydrolysates have been suggested as a favorable antiaging modality in skin photoaged by persistent exposure to ultraviolet radiation (UV). The current study evaluated the beneficial effect of collagen hydrolysates (fsCH) extracted from Pangasius hypophthalmus fish skin on wrinkle formation and moisture preservation in dorsal skin of hairless mice challenged with UV-B. Inter-comparative experiments were conducted for anti-photoaging among fsCH, retinoic acid (RA), N-acetyl-D-glucosamine (NAG), and glycine-proline-hydroxyproline (GPH). Treating human HaCaT keratinocytes with 100−200 μg/mL fsCH reciprocally ameliorated the expression of aquaporin 3 (AQP3) and CD44 deranged by UV-B. The UV-B-induced deep furrows and skin thickening were improved in parched dorsal skin of mice supplemented with 206–412 mg/kg fsCH as well as RA and GPH. The UV-B irradiation enhanced collagen fiber loss in the dorsal dermis, which was attenuated by fsCH through enhancing procollagen conversion to collagen. The matrix metalloproteinase expression by UV-B in dorsal skin was diminished by fsCH, similar to RA and GPH, via blockade of collagen degradation. Supplementing fsCH to UV-B-irradiated mice decreased transepidermal water loss in dorsal skin with reduced AQP3 level and restored keratinocyte expression of filaggrin. The expression of hyaluronic acid synthase 2 and hyaluronidase 1 by UV-B was remarkably ameliorated with increased production of hyaluronic acid by treating fsCH to photoaged mice. Taken together, fsCH attenuated photoaging typical of deep wrinkles, epidermal thickening, and skin water loss, like NAG, RA, or GPH, through inhibiting collagen destruction and epidermal barrier impairment.

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(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/2761463 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 8

Author(s):

Bonggi Lee ◽

Kyoung Mi Moon ◽

Seong Jin Kim ◽

So Hee Kim ◽

Dae Hyun Kim ◽

...

Keyword(s):

Oxidative Stress ◽

Collagen Fiber ◽

Enzyme Linked Immunosorbent Assay ◽

Molecular Signaling ◽

Dorsal Skin ◽

Hairless Mice ◽

Protein Levels ◽

Uvb Exposure

Background. Uncontrolled melanogenesis and wrinkle formation are an indication of photoaging. Our previous studies demonstrated that (Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione (MHY498) inhibited tyrosinase activity and melanogenesisin vitro.Objective. To examinein vivoeffects of MHY498 as an antiaging compound on UVB-induced melanogenesis and wrinkle formation, we topically applied MHY498 on dorsal skin of HRM-2 hairless mice.Methods. Using histological analysis, we evaluated effects of MHY498 on melanogenesis and wrinkle formation after UVB exposure. In addition, related molecular signaling pathways were examined using western blotting, fluorometric assay, and enzyme-linked immunosorbent assay.Results. MHY498 suppressed UVB-induced melanogenesis by inhibiting phosphorylation of CREB and translocation of MITF protein into the nucleus, which are key factors for tyrosinase expression. Consistently, tyrosinase protein levels were notably reduced in the dorsal skin of the hairless mice by MHY498 treatment. Furthermore, MHY498 inhibited UVB-induced wrinkle formation and collagen fiber destruction by increasing type 1 procollagen concentration and decreasing protein expression levels of MMPs, which play an essential role in collagen fiber degradation. As a mechanism, MHY498 notably ameliorated UVB-induced oxidative stress and NF-κB activation in the dermal skin of the hairless mice.Conclusion. Our study suggests that MHY498 can be used as a therapeutic or cosmetic agent for preventing uncontrolled melanogenesis and wrinkle formation.

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Anti-Atopic Properties of Gracillin Isolated from Dioscorea quinqueloba on 2,4-Dinitrochlorobenzene-Induced Skin Lesions in Mice

Nutrients ◽

10.3390/nu10091205 ◽

2018 ◽

Vol 10 (9) ◽

pp. 1205 ◽

Cited By ~ 7

Author(s):

Jonghwan Jegal ◽

No-June Park ◽

Beom-Geun Jo ◽

Sim-Kyu Bong ◽

Hyun Jegal ◽

...

Keyword(s):

Atopic Dermatitis ◽

Water Loss ◽

Skin Lesions ◽

Transepidermal Water Loss ◽

Skin Hydration ◽

Potential Candidate ◽

Immunomodulatory Effects ◽

Hairless Mice ◽

Naturally Occurring ◽

Prevention And Treatment

Naturally occurring saponins have been reported to have anti-inflammatory and immunomodulatory effects. However, the effects of gracillin, a main saponin component of Dioscorea quinqueloba (D. quinqueloba), on atopic dermatitis (AD), have not been previously studied. The aim of this study was to determine whether gracillin isolated from D. quinqueloba has an anti-AD effect on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in SKH-1 hairless mice. Topical co-treatment of gracillin and DNCB for two weeks markedly reduced symptoms typical of AD (redness, itching, swelling and skin lichenification), decreased transepidermal water loss (TEWL) and increased skin hydration. In addition, gracillin strongly inhibited PI-induced IL-4 expression in RBL-2H3 cells and in the skins of AD mice. Our results suggest gracillin is a potential candidate for the prevention and treatment of AD and other inflammatory skin disorders.

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Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice

Marine Drugs ◽

10.3390/md19100550 ◽

2021 ◽

Vol 19 (10) ◽

pp. 550

Author(s):

Jung Eun Seol ◽

Sang Woo Ahn ◽

Bomin Seol ◽

Hyeong Rok Yun ◽

Nammi Park ◽

...

Keyword(s):

Water Loss ◽

Inflammatory Cell ◽

Transepidermal Water Loss ◽

Collagen Degradation ◽

Ultraviolet B ◽

Inflammatory Cell Infiltration ◽

Cell Infiltration ◽

Skin Barrier Function ◽

Hairless Mice ◽

Echinochrome A

Echinochrome A (Ech A, 7-ethyl-2,3,5,6,8-pentahydroxy-1,4-naphthoquinone) has been known to exhibit anti-oxidative and anti-inflammatory effects. However, no study has been carried out on the efficacy of Ech A against skin photoaging; this process is largely mediated by oxidative stress. Six-week-old male SKH-1 hairless mice (n = 36) were divided into five groups. Except for a group that were not treated (n = 4), all mice underwent ultraviolet-B (UVB) exposure for 8 weeks while applying phosphate-buffered saline or Ech A through intraperitoneal injection. UVB impaired skin barrier function, showing increased transepidermal water loss and decreased stratum corneum hydration. UVB induced dermal collagen degeneration and mast cell infiltration. Ech A injection was found to significantly lower transepidermal water loss while attenuating tissue inflammatory changes and collagen degeneration compared to the control. Furthermore, Ech A was found to decrease the relative expression of matrix metalloproteinase, tryptase, and chymase. Taken together, these results suggest that Ech A protects against UVB-induced photoaging in both functional and histologic aspects, causing a lowering of collagen degradation and inflammatory cell infiltration.

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Impact of Ultraviolet Radiation and Ozone on the Transepidermal Water Loss as a Function of Skin Temperature in Hairless Mice

Skin Pharmacology and Physiology ◽

10.1159/000072068 ◽

2003 ◽

Vol 16 (5) ◽

pp. 283-290 ◽

Cited By ~ 22

Author(s):

J.J. Thiele ◽

F. Dreher ◽

H.I. Maibach ◽

L. Packer

Keyword(s):

Ultraviolet Radiation ◽

Skin Temperature ◽

Water Loss ◽

Transepidermal Water Loss ◽

Hairless Mice

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The examination of biophysical parameters of skin (transepidermal water loss, skin hydration and pH value) in different body regions of ponies

Polish Journal of Veterinary Sciences ◽

10.2478/v10181-012-0081-8 ◽

2012 ◽

Vol 15 (3) ◽

pp. 553-559 ◽

Cited By ~ 4

Author(s):

M.P. Szczepanik ◽

P.M. Wilkołek ◽

M. Pluta ◽

Ł.R. Adamek ◽

Z.J.H. Pomorski

Keyword(s):

Water Loss ◽

Ph Value ◽

Neck Region ◽

Transepidermal Water Loss ◽

Skin Hydration ◽

Lumbar Region ◽

Biophysical Parameters ◽

Body Regions ◽

Skin Ph

Abstract The purpose of this study was to evaluate transepidermal water loss, skin hydration and skin pH in normal ponies. Sixteen ponies of both sexes were examined in the study. Measurements were taken from seven different sites: the neck region, the shoulder, thorax, lumbar, inguinal, lip region and the auricle. In each of the regions transepidermal water loss (TEWL), skin hydration and skin pH were measured. For transepidermal water loss, the lowest values were observed in the lumbar region (9.71g/hm2), while the highest values were observed in the lip region (22.35 g/hm2). In the case of skin hydration the lowest values were observed for the thorax region (2.13 CU), and the highest for the lip region (41.81 CU). For skin pH, the lowest results were obtained in the lumbar region (6.93), and the highest in the lip region (7.96).

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