Faculty Opinions recommendation of Influence of Aqueous Cream BP on corneocyte size, maturity, skin protease activity, protein content and transepidermal water loss.

2011 ◽  
Author(s):  
Joyce Lee ◽  
Lucinda Tan
Keyword(s):  
Protein Content ◽  
Water Loss ◽  
Protease Activity ◽  
Transepidermal Water Loss

Extraction and Partial Purification of Protease from Bilimbi (Averrhoa bilimbi L.)

2013 ◽  
Vol 10 (2) ◽  
pp. 29
Author(s):  
Normah Ismail ◽  
Nur' Ain Mohamad Kharoe
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
Ammonium Sulfate ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Protease Activity ◽  
Protein Concentration ◽  
Temperature Stability ◽  
Partial Purification ◽  
Ammonium Sulfate Precipitation

Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of 40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity of both the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between IO to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. 

The examination of biophysical parameters of skin (transepidermal water loss, skin hydration and pH value) in different body regions of ponies

2012 ◽  
Vol 15 (3) ◽  
pp. 553-559 ◽  
Author(s):  
M.P. Szczepanik ◽  
P.M. Wilkołek ◽  
M. Pluta ◽  
Ł.R. Adamek ◽  
Z.J.H. Pomorski
Keyword(s):  
Water Loss ◽  
Ph Value ◽  
Neck Region ◽  
Transepidermal Water Loss ◽  
Skin Hydration ◽  
Lumbar Region ◽  
Biophysical Parameters ◽  
Body Regions ◽  
Skin Ph

Abstract The purpose of this study was to evaluate transepidermal water loss, skin hydration and skin pH in normal ponies. Sixteen ponies of both sexes were examined in the study. Measurements were taken from seven different sites: the neck region, the shoulder, thorax, lumbar, inguinal, lip region and the auricle. In each of the regions transepidermal water loss (TEWL), skin hydration and skin pH were measured. For transepidermal water loss, the lowest values were observed in the lumbar region (9.71g/hm2), while the highest values were observed in the lip region (22.35 g/hm2). In the case of skin hydration the lowest values were observed for the thorax region (2.13 CU), and the highest for the lip region (41.81 CU). For skin pH, the lowest results were obtained in the lumbar region (6.93), and the highest in the lip region (7.96).

scholarly journals Monitoring Transepidermal Water Loss and Skin Wettedness Factor with Battery-Free NFC Sensor

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5549
Author(s):  
Syed Muhammad Ali ◽  
Wan-Young Chung
Keyword(s):  
Water Loss ◽  
Near Field ◽  
Experimental Tests ◽  
Transepidermal Water Loss ◽  
Comfort Level ◽  
Skin Wettedness ◽  
Moisture Content Level ◽  
Indoor And Outdoor Environments ◽  
Skin Moisture

The transepidermal water loss (TEWL) and the skin wettedness factor (SWF) are considered parts of a key perspective related to skincare. The former is used to determine the loss of water content from the stratum corneum (SC), while the latter is used to determine the human skin comfort level. Herein, we developed two novel approaches: (1) determination of the TEWL and the SWF based on a battery-free humidity sensor, and (2) the design of a battery-free smart skincare sensor device tag that can harvest energy from a near field communication (NFC)-enabled smartphone, making it a battery-free design approach. The designed skincare device tag has a diameter of 2.6 cm and could harvest energy (~3 V) from the NFC-enabled smartphone. A series of experimental tests involving the participation of eight and six subjects were conducted in vivo for the indoor and outdoor environments, respectively. During the experimental analysis, the skin moisture content level was measured at different times of the day using an android smartphone. The TEWL and SWF values were calculated based on these sensor readings. For the TEWL case: if the skin moisture is high, the TEWL is high, and if the skin moisture is low, the TEWL is low, ensuring that the skin moisture and the TEWL follow the same trend. Our smart skincare device is enclosed in a 3D flexible design print, and it is battery-free with an android application interface that is more convenient to carry outside than other commercially available battery-based devices.

scholarly journals Skin Barrier Function in Psoriasis and Atopic Dermatitis: Transepidermal Water Loss and Temperature as Useful Tools to Assess Disease Severity

2021 ◽  
Vol 10 (2) ◽  
pp. 359 ◽  
Author(s):  
Trinidad Montero-Vilchez ◽  
María-Victoria Segura-Fernández-Nogueras ◽  
Isabel Pérez-Rodríguez ◽  
Miguel Soler-Gongora ◽  
Antonio Martinez-Lopez ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Disease Severity ◽  
Water Loss ◽  
Barrier Function ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Diagnostic Tools ◽  
Psoriatic Skin ◽  
Skin Barrier Function ◽  
Healthy Controls

Multiple diagnostic tools are used to evaluate psoriasis and atopic dermatitis (AD) severity, but most of them are based on subjective components. Transepidermal water loss (TEWL) and temperature are skin barrier function parameters that can be objectively measured and could help clinicians to evaluate disease severity accurately. Thus, the aims of this study are: (1) to compare skin barrier function between healthy skin, psoriatic skin and AD skin; and (2) to assess if skin barrier function parameters could predict disease severity. A cross-sectional study was designed, and epidermal barrier function parameters were measured. The study included 314 participants: 157 healthy individuals, 92 psoriatic patients, and 65 atopic dermatitis patients. TEWL was significantly higher, while stratum corneum hydration (SCH) (8.71 vs. 38.43 vs. 44.39 Arbitrary Units (AU)) was lower at psoriatic plaques than at uninvolved psoriatic skin and healthy controls. Patients with both TEWL > 13.85 g·m−2h−1 and temperature > 30.85 °C presented a moderate/severe psoriasis (psoriasis area severity index (PASI) ≥ 7), with a specificity of 76.3%. TEWL (28.68 vs. 13.15 vs. 11.60 g·m−2 h−1) and temperature were significantly higher, while SCH (25.20 vs. 40.95 vs. 50.73 AU) was lower at AD eczematous lesions than uninvolved AD skin and healthy controls. Patients with a temperature > 31.75 °C presented a moderate/severe AD (SCORing Atopic Dermatitis (SCORAD) ≥ 37) with a sensitivity of 81.8%. In conclusion, temperature and TEWL values may help clinicians to determine disease severity and select patients who need intensive treatment.

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