scholarly journals Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS)

2021 ◽  
Vol 22 (14) ◽  
pp. 7629
Author(s):  
Chiara Magri ◽  
Erika Vitali ◽  
Sara Cocco ◽  
Edoardo Giacopuzzi ◽  
Marco Rinaudo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Direct Current ◽  
Transcranial Direct Current Stimulation ◽  
Whole Blood ◽  
Point Of View ◽  
Direct Current Stimulation ◽  
Transcriptomic Level ◽  
New Biomarkers ◽  
Blood Transcriptome

The 3xTg-AD mouse is a widely used model in the study of Alzheimer’s Disease (AD). It has been extensively characterized from both the anatomical and behavioral point of view, but poorly studied at the transcriptomic level. For the first time, we characterize the whole blood transcriptome of the 3xTg-AD mouse at three and six months of age and evaluate how its gene expression is modulated by transcranial direct current stimulation (tDCS). RNA-seq analysis revealed 183 differentially expressed genes (DEGs) that represent a direct signature of the genetic background of the mouse. Moreover, in the 6-month-old 3xTg-AD mice, we observed a high number of DEGs that could represent good peripheral biomarkers of AD symptomatology onset. Finally, tDCS was associated with gene expression changes in the 3xTg-AD, but not in the control mice. In conclusion, this study provides an in-depth molecular characterization of the 3xTg-AD mouse and suggests that blood gene expression can be used to identify new biomarkers of AD progression and treatment effects.

scholarly journals Differences in functional connectivity distribution after transcranial direct-current stimulation: a connectivity density point of view

2020 ◽  
Author(s):  
Bohao Tang ◽  
Yi Zhao ◽  
Archana Venkataraman ◽  
Kyrana Tsapkini ◽  
Martin A Lindquist ◽  
...  
Keyword(s):  
Functional Connectivity ◽  
Direct Current ◽  
Transcranial Direct Current Stimulation ◽  
Primary Progressive Aphasia ◽  
Point Of View ◽  
Language Therapy ◽  
Post Intervention ◽  
Direct Stimulation ◽  
Direct Current Stimulation ◽  
The Impact

AbstractIn this manuscript we consider the problem of relating functional connectivity measurements viewed as statistical distributions to outcomes. We demonstrate the utility of using the distribution of connectivity on a study of resting state functional magnetic resonance imaging association with an intervention. Specifically, we consider 47 primary progressive aphasia (PPA) patients with various levels of language abilities. These patients were randomly assigned to two treatment arms, tDCS (transcranial direct-current stimulation and language therapy) vs sham (language therapy only), in a clinical trial. We propose a novel approach to analyze the effect of direct stimulation on functional connectivity. We estimate the density of correlations among the regions of interest (ROIs) and study the difference in the density post-intervention between treatment arms. We discover that it is the tail of the density, rather than the mean or lower order moments of the distribution, that demonstrates a significant impact in the classification. This approach has several benefits. Among them, it drastically reduces the number of multiple comparisons compared to edge-wise analysis. In addition, it allows for the investigation of the impact of functional connectivity on the outcomes where the connectivity is not geometrically localized.

scholarly journals Dynamic DNA Methylation Changes in the COMT Gene Promoter Region in Response to Mental Stress and Its Modulation by Transcranial Direct Current Stimulation

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1726
Author(s):  
Ariane Wiegand ◽  
Arne Blickle ◽  
Christof Brückmann ◽  
Simone Weller ◽  
Vanessa Nieratschker ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Direct Current ◽  
Transcranial Direct Current Stimulation ◽  
Whole Blood ◽  
Molecular Mechanisms ◽  
Cognitive Task ◽  
Gene Promoter ◽  
Continuous Increase ◽  
Direct Current Stimulation ◽  
Concentration Changes

Changes in epigenetic modifications present a mechanism how environmental factors, such as the experience of stress, can alter gene regulation. While stress-related disorders have consistently been associated with differential DNA methylation, little is known about the time scale in which these alterations emerge. We investigated dynamic DNA methylation changes in whole blood of 42 healthy male individuals in response to a stressful cognitive task, its association with concentration changes in cortisol, and its modulation by transcranial direct current stimulation (tDCS). We observed a continuous increase in COMT promotor DNA methylation which correlated with higher saliva cortisol levels and was still detectable one week later. However, this lasting effect was suppressed by concurrent activity-enhancing anodal tDCS to the dorsolateral prefrontal cortex. Our findings support the significance of gene-specific DNA methylation in whole blood as potential biomarkers for stress-related effects. Moreover, they suggest alternative molecular mechanisms possibly involved in lasting behavioral effects of tDCS.

Acceptability of transcranial direct current stimulation in children and adolescents with ADHD: The point of view of parents

2020 ◽  
pp. 135910532093705 ◽  
Author(s):  
Derrick Matthew Buchanan ◽  
Amedeo D’Angiulli ◽  
André Samson ◽  
Alexander R Maisonneuve ◽  
Philippe Robaey
Keyword(s):  
Direct Current ◽  
Transcranial Direct Current Stimulation ◽  
Point Of View ◽  
Theory Approach ◽  
Care Providers ◽  
Direct Current Stimulation ◽  
Hyperactivity Disorder ◽  
Novel Treatment ◽  
Treatment Education ◽  
Grounded Theory Approach

Transcranial direct current stimulation (tDCS) is a novel treatment option for attention deficit hyperactivity disorder. To facilitate translation into clinical practice, we interviewed parents of children who have experienced experimental tDCS. A grounded theory approach using open, axial, and selective coding provided seven emergent themes for acceptability: tDCS provides hope for parents, safety tolerability and side effects of tDCS versus medication, burden of treatment, education and trust with care providers, cost and coverage, unestablished tDCS efficacy versus established medication effectiveness, perceived compliance of tDCS versus medication. Results suggest tDCS is acceptable but depends on evidence of effectiveness and regular availability.

scholarly journals Dynamic DNA methylation changes in the COMT gene promoter region in response to mental stress and its modulation by transcranial direct current stimulation

2021 ◽  
Author(s):  
Ariane Wiegand ◽  
Arne Blickle ◽  
Christof Brückmann ◽  
Simone Weller ◽  
Vanessa Nieratschker ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Direct Current ◽  
Transcranial Direct Current Stimulation ◽  
Whole Blood ◽  
Molecular Mechanisms ◽  
Cognitive Task ◽  
Gene Promoter ◽  
Continuous Increase ◽  
Direct Current Stimulation ◽  
Concentration Changes

AbstractChanges in epigenetic modifications present a mechanism how environmental factors like the experience of stress can alter gene regulation. While stress-related disorders have consistently been associated with differential DNA methylation, little is known about the time scale in which these alterations emerge. We investigated dynamic DNA methylation changes in whole blood of 42 healthy male individuals in response to a stressful cognitive task, its association with concentration changes in cortisol and its modulation by transcranial direct current stimulation (tDCS). We observed a continuous increase in COMT promotor DNA methylation which correlated with higher saliva cortisol levels and was still detectable one week later. However, this lasting effect was suppressed by concurrent activity-enhancing anodal tDCS to the dorsolateral prefrontal cortex. Our findings support the significance of gene-specific DNA methylation in whole blood as potential biomarkers for stress-related disorders. Moreover, they suggest alternative molecular mechanisms possibly involved in lasting behavioral effects of tDCS.

Transcranial direct current stimulation and retraining in musician's hand dystonia

2009 ◽  
Vol 40 (01) ◽  
Author(s):  
F Buttkus ◽  
V Baur ◽  
HC Jabusch ◽  
M Weidenmüller ◽  
S Schneider ◽  
...  
Keyword(s):  
Direct Current ◽  
Transcranial Direct Current Stimulation ◽  
Direct Current Stimulation ◽  
Hand Dystonia

Bifrontale transkranielle Gleichstromstimulation zur Behandlung der Schizophrenie

2014 ◽  
Vol 33 (05) ◽  
pp. 382-386
Author(s):  
A. Hasan ◽  
S. Dehning ◽  
D. Keeser ◽  
I. Hummel ◽  
P. Falkai ◽  
...  
Keyword(s):  
Direct Current ◽  
Transcranial Direct Current Stimulation ◽  
Sich Eine ◽  
Direct Current Stimulation ◽  
Klinische Relevanz ◽  
Transkranielle Gleichstromstimulation

Zusammenfassung Gegenstand und Ziel: Die transkranielle Gleichstromstimulation (transcranial direct current stimulation, tDCS) wird als neues nicht invasives Hirnstimulationsverfahren für die Behandlung neurologischer und psychiatrischer Erkrankungen untersucht. Mittlerweile gibt es Fallberichte und eine randomisierte, kontrollierte Pilotstudie zur Behandlung akustischer Halluzinationen sowie der Negativsymptomatik bei Schizophrenie. Fallbericht: Im vorliegenden Fallbericht wurde ein Patient mit therapieresistenter Symptomatik bei paranoider Schizophrenie drei Wochen lang mit anodaler tDCS über dem linken dorsolateralen und kathodaler tDCS über dem rechten dorsolateralen Kortex behandelt. Ergebnisse: Es zeigte sich eine Verbesserung von Affektivität und Kognition, die Positivsymptome blieben jedoch nahezu unverändert. Klinische Relevanz: Die bifrontale tDCS stellt eine nebenwirkungsarme neue Behandlungsform dar und sollte in der Anwendung bei Erkrankungen aus dem schizophrenen Formenkreis weiter untersucht werden.

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