scholarly journals High Resolution Analysis of Proteome Dynamics during Bacillus subtilis Sporulation

2021 ◽  
Vol 22 (17) ◽  
pp. 9345
Author(s):  
Zhiwei Tu ◽  
Henk L. Dekker ◽  
Winfried Roseboom ◽  
Bhagyashree N. Swarge ◽  
Peter Setlow ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
High Resolution ◽  
Nutrient Limitation ◽  
Time Lapse ◽  
Regulatory Proteins ◽  
Coat Proteins ◽  
Resolution Time ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
A Cell

Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome dynamics during sporulation, high-resolution time-lapse proteomics was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively investigate the changeover of sporulation regulatory proteins, coat proteins, and other proteins involved in sporulation and spore biogenesis. Protein co-expression analysis revealed four co-expressed modules (termed blue, brown, green, and yellow). Modules brown and green are upregulated during sporulation and contain proteins associated with sporulation. Module blue is negatively correlated with modules brown and green, containing ribosomal and metabolic proteins. Finally, module yellow shows co-expression with the three other modules. Notably, several proteins not belonging to any of the known transcription regulons were identified as co-expressed with modules brown and green, and might also play roles during sporulation. Finally, levels of some coat proteins, for example morphogenetic coat proteins, decreased late in sporulation.

scholarly journals High resolution analysis of proteome dynamics during Bacillus subtilis sporulation

2021 ◽  
Author(s):  
Zhiwei Tu ◽  
Henk Dekker ◽  
Winfried Roseboom ◽  
Bhagyashree Swarge ◽  
Peter Setlow ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
High Resolution ◽  
Nutrient Limitation ◽  
Time Lapse ◽  
Regulatory Proteins ◽  
Coat Proteins ◽  
Resolution Time ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
A Cell

Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome dynamics during sporulation, high resolution time-lapse proteomics was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively investigate the changeover of sporulation regulatory proteins, coat proteins and other proteins involved in sporulation and spore biogenesis. Protein co-expression analysis revealed four co-expressed modules (blue, brown, green and yellow). Modules brown and green are upregulated during sporulation and contain proteins associated with sporulation. Module blue, is negatively correlated with modules brown and green, and contained ribosomal and metabolic proteins. Finally, module yellow shows co-expression with the three other modules. Notably, several proteins not belonging to any of the known transcription regulons were identified as co-expressed with modules brown and green. We speculate that they may also play roles during sporulation. Finally, levels of some coat proteins, for example morphogenetic coat proteins, decreased late in sporulation. We speculate on their possible role in guiding or helping assembly of other coat proteins, after which they can be disposed of, but such a hypothesis remains to be experimentally addressed.

Very High Resolution Analysis of the Dynamics of a Coronal Plasmoid

1994 ◽  
Vol 144 ◽  
pp. 593-596
Author(s):  
O. Bouchard ◽  
S. Koutchmy ◽  
L. November ◽  
J.-C. Vial ◽  
J. B. Zirker
Keyword(s):  
High Resolution ◽  
Solar Eclipse ◽  
Total Solar Eclipse ◽  
Field Of View ◽  
Small Field ◽  
High Resolution Analysis ◽  
Ccd Observations ◽  
Resolution Analysis ◽  
Very High ◽  
Small Field Of View

AbstractWe present the results of the analysis of a movie taken over a small field of view in the intermediate corona at a spatial resolution of 0.5“, a temporal resolution of 1 s and a spectral passband of 7 nm. These CCD observations were made at the prime focus of the 3.6 m aperture CFHT telescope during the 1991 total solar eclipse.

scholarly journals How to Effectively Control Energy Consumption Growth in China’s 29 Provinces: A Paradigm of Multi-Regional Analysis Based on EAALMDI Method

2021 ◽  
Vol 13 (3) ◽  
pp. 1093
Author(s):  
Yunlong Zhao ◽  
Geng Kong ◽  
Chin Hao Chong ◽  
Linwei Ma ◽  
Zheng Li ◽  
...  
Keyword(s):  
Energy Consumption ◽  
High Resolution ◽  
Energy Policy ◽  
Policy Making ◽  
Regional Analysis ◽  
Policy Implications ◽  
Consumption Growth ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Consumption Control

Controlling energy consumption to reduce greenhouse gas emissions has become a global consensus in response to the challenge of climate change. Most studies have focused on energy consumption control in a single region; however, high-resolution analysis of energy consumption and personalized energy policy-making, for multiple regions with differentiated development, have become a complicated challenge. Using the logarithmic mean Divisia index I (LMDI) decomposition method based on energy allocation analysis (EAA), this paper aims to establish a standard paradigm for a high-resolution analysis of multi-regional energy consumption and provide suggestions for energy policy-making, taking 29 provinces of China as the sample. The process involved three steps: (1) determination of regional priorities of energy consumption control by EAA, (2) revealing regional disparity among the driving forces of energy consumption growth by LMDI, and (3) deriving policy implications by comparing the obtained results with existing policies. The results indicated that 29 provinces can be divided into four groups, with different priorities of energy consumption control according to the patterns of coal flows. Most provinces have increasing levels of energy consumption, driven by increasing per capita GDP and improving living standards, while its growth is restrained by decreasing end-use energy intensity, improving energy supply efficiency, and optimization of industrial structures. However, some provinces are not following these trends to the same degree. This indicates that policy-makers must pay more attention to the different driving mechanisms of energy consumption growth among provinces.

scholarly journals A Back Migration from Asia to Sub-Saharan Africa Is Supported by High-Resolution Analysis of Human Y-Chromosome Haplotypes

2002 ◽  
Vol 70 (5) ◽  
pp. 1197-1214 ◽  
Author(s):  
Fulvio Cruciani ◽  
Piero Santolamazza ◽  
Peidong Shen ◽  
Vincent Macaulay ◽  
Pedro Moral ◽  
...  
Keyword(s):  
High Resolution ◽  
Y Chromosome ◽  
Sub Saharan Africa ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Sub Saharan ◽  
Human Y Chromosome

scholarly journals Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Aleksandar Antanasijevic ◽  
Leigh M. Sewall ◽  
Christopher A. Cottrell ◽  
Diane G. Carnathan ◽  
Luis E. Jimenez ◽  
...  
Keyword(s):  
High Resolution ◽  
Polyclonal Antibody ◽  
Vaccine Development ◽  
Mutational Analysis ◽  
Rhesus Macaques ◽  
Antibody Responses ◽  
Glycosylation Sites ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Resolution Mapping

AbstractEngineered ectodomain trimer immunogens based on BG505 envelope glycoprotein are widely utilized as components of HIV vaccine development platforms. In this study, we used rhesus macaques to evaluate the immunogenicity of several stabilized BG505 SOSIP constructs both as free trimers and presented on a nanoparticle. We applied a cryoEM-based method for high-resolution mapping of polyclonal antibody responses elicited in immunized animals (cryoEMPEM). Mutational analysis coupled with neutralization assays were used to probe the neutralization potential at each epitope. We demonstrate that cryoEMPEM data can be used for rapid, high-resolution analysis of polyclonal antibody responses without the need for monoclonal antibody isolation. This approach allowed to resolve structurally distinct classes of antibodies that bind overlapping sites. In addition to comprehensive mapping of commonly targeted neutralizing and non-neutralizing epitopes in BG505 SOSIP immunogens, our analysis revealed that epitopes comprising engineered stabilizing mutations and of partially occupied glycosylation sites can be immunogenic.

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