scholarly journals TaWAK2A-800, a Wall-Associated Kinase, Participates Positively in Resistance to Fusarium Head Blight and Sharp Eyespot in Wheat

2021 ◽  
Vol 22 (21) ◽  
pp. 11493
Author(s):  
Feilong Guo ◽  
Tianci Wu ◽  
Gangbiao Xu ◽  
Haijun Qi ◽  
Xiuliang Zhu ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Pathogenic Fungi ◽  
Transcript Abundance ◽  
Marker Genes ◽  
Gene Transcript ◽  
Head Blight ◽  
Wheat Varieties ◽  
Sharp Eyespot ◽  
Immune Pathway ◽  
Cereal Plants

Fusarium head blight (FHB) and sharp eyespot are important diseases of the cereal plants, including bread wheat (Triticum aestivum) and barley. Both diseases are predominately caused by the pathogenic fungi, Fusarium graminearum and Rhizoctonia cerealis. The roles of the wheat-wall-associated kinases (WAKs) in defense against both F. graminearum and R. cerealis have remained largely unknown. This research reports the identification of TaWAK2A-800, a wheat WAK-coding gene located on chromosome 2A, and its functional roles in wheat resistance responses to FHB and sharp eyespot. TaWAK2A-800 transcript abundance was elevated by the early infection of R. cerealis and F. graminearum, or treatment with exogenous chitin. The gene transcript seemed to correspond to the resistance of wheat. Further functional analyses showed that silencing TaWAK2A-800 compromised the resistance of wheat to both FHB (F. graminearum) and sharp eyespot (R. cerealis). Moreover, the silencing reduced the expression levels of six defense-related genes, including the chitin-triggering immune pathway-marker genes, TaCERK1, TaRLCK1B, and TaMPK3. Summarily, TaWAK2A-800 participates positively in the resistance responses to both F. graminearum and R. cerealis, possibly through a chitin-induced pathway in wheat. TaWAK2A-800 will be useful for breeding wheat varieties with resistance to both FHB and sharp eyespot.

scholarly journals Type II Fusarium head blight susceptibility conferred by a region on wheat chromosome 4D

2020 ◽  
Vol 71 (16) ◽  
pp. 4703-4714 ◽  
Author(s):  
Benjamin Hales ◽  
Andrew Steed ◽  
Vincenzo Giovannelli ◽  
Christopher Burt ◽  
Marc Lemmens ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Chinese Spring ◽  
Wheat Chromosome ◽  
Addition Line ◽  
Head Blight ◽  
Wheat Varieties ◽  
Susceptibility Factor ◽  
Fhb Resistance ◽  
Grain Yield And Quality ◽  
Wheat Lines

Abstract Fusarium head blight (FHB) causes significant grain yield and quality reductions in wheat and barley. Most wheat varieties are incapable of preventing FHB spread through the rachis, but disease is typically limited to individually infected spikelets in barley. We point-inoculated wheat lines possessing barley chromosome introgressions to test whether FHB resistance could be observed in a wheat genetic background. The most striking differential was between 4H(4D) substitution and 4H addition lines. The 4H addition line was similarly susceptible to the wheat parent, but the 4H(4D) substitution line was highly resistant, which suggests that there is an FHB susceptibility factor on wheat chromosome 4D. Point inoculation of Chinese Spring 4D ditelosomic lines demonstrated that removing 4DS results in high FHB resistance. We genotyped four Chinese Spring 4DS terminal deletion lines to better characterize the deletions in each line. FHB phenotyping indicated that lines del4DS-2 and del4DS-4, containing smaller deletions, were susceptible and had retained the susceptibility factor. Lines del4DS-3 and del4DS-1 contain larger deletions and were both significantly more resistant, and hence had presumably lost the susceptibility factor. Combining the genotyping and phenotyping results allowed us to refine the susceptibility factor to a 31.7 Mbp interval on 4DS.

scholarly journals Characterization of Agronomical and Quality Traits of Winter Wheat (Triticum aestivum L.) for Fusarium Head Blight Pressure in Different Environments

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 213
Author(s):  
Valentina Spanic ◽  
Josipa Cosic ◽  
Zvonimir Zdunic ◽  
Georg Drezner
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Grain Yield ◽  
Fusarium Head Blight ◽  
Total Variation ◽  
Environmental Conditions ◽  
Triticum Aestivum L ◽  
Test Weight ◽  
Head Blight ◽  
Wheat Varieties

For food security, it is essential to identify stable, high-yielding wheat varieties with lower disease severity. This is particularly important due to climate change, which results in pressure due to the increasing occurrence of Fusarium head blight (FHB). The objective of this study was to evaluate the stability of winter wheat (Triticum aestivum L.) grain yield under different environmental conditions. Twenty-five winter wheat varieties were evaluated under two treatments (naturally-disease infected (T1) and FHB artificial stress (T2)) during two growing seasons (2018–2019 to 2019–2020) in Osijek and in 2019–2020 in Tovarnik. The interaction between varieties and different environments for grain yield was described using the additive main-effects and multiplicative interaction (AMMI) effects model. The Kraljica and Fifi varieties were located near the origin of the biplot, thus indicating non-sensitivity to different environmental conditions. Principal component analysis (PCA) was used to understand the trait and environmental relationships. PC1 alone contributed 42.5% of the total variation, which was mainly due to grain yield, 1000 kernel weight and test weight in that respective order. PC2 contributed 21.1% of the total variation mainly through the total sedimentation value, test weight, wet gluten and protein content ratio (VG/P) and wet gluten content, in descending order.

scholarly journals Susceptibility of non-cereal crops to Fusarium graminearum complex and their role within cereal crop rotation as a source of inoculum for Fusarium head blight

2019 ◽  
Vol 16 (4) ◽  
pp. e1012 ◽  
Author(s):  
Neringa Rasiukeviciute ◽  
Skaidre Suproniene ◽  
Jurgita Kelpsiene ◽  
Povilas Svegzda ◽  
Grazina Kadziene ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Fusarium Head Blight ◽  
Crop Rotation ◽  
Fusarium Graminearum ◽  
Oilseed Rape ◽  
Spring Wheat ◽  
Faba Bean ◽  
Cereal Crop ◽  
Head Blight ◽  
Cereal Plants

Fusarium graminearum, the cause of Fusarium head blight (FHB), is an important cereal pathogen. Moreover, some non-graminaceous crops are also known to be susceptible to F. graminearum infection. This study assessed the presence of F. graminearum species complex on non-cereal plants, grown in a cereal crop rotation and evaluated its pathogenicity to non-cereal plants in vitro and to spring wheat under field conditions. The relative density of Fusarium species isolated from oilseed rape, pea, potato and sugar beet plants was assessed in 2015 and 2016. A total of 403 isolates of Fusarium spp. were obtained from non-cereal plants and only 5% of the isolates were identified as F. graminearum. The pathogenicity test revealed that isolates of F. graminearum from spring wheat and non-cereal plants caused discolourations on leaves of faba bean, fodder beet, oilseed rape, pea, potato and sugar beet. The pea was the crop most susceptible to F. graminearum isolated from spring wheat. The pathogenicity of F. graminearum from sugar beet, oilseed rape, pea and potato to the same hosts differed depending on isolate and inoculated plant. Under field conditions, F. graminearum isolates from pea, potato, oilseed rape and wild viola were able to cause typical FHB symptoms in spring wheat. Based on the information generated in this study, we conclude that under congenial conditions, growing faba bean, pea, sugar beet, fodder beet, oilseed rape and potato plants in a cereal crop rotation may serve as alternative or reservoir hosts for F. graminearum pathogens.

scholarly journals Assessment of Agrocenosis Adaptability of Winter Wheat in the Conditions of the Foothill Zone of the Central Caucasus

2021 ◽  
Author(s):  
Irina Manukyan ◽  
Elena Miroshnikova ◽  
Madina Basieva ◽  
Nino Doguzova
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Leaf Beetle ◽  
Tan Spot ◽  
Head Blight ◽  
Wheat Varieties ◽  
Cereal Leaf Beetle ◽  
Oulema Melanopus ◽  
Central Caucasus ◽  
Selection Of

This paper presents the results of multi-year research on the adaptability of winter wheat varieties to the conditions of the foothill zone of the Central Caucasus. Characteristics such as ductility and stability were used to assess varieties for conformity to the cultivation conditions. Plant homeostaticity served as the indicator of ontogenetic adaptability. Phenological observations and reports were carried out according to the state variety testing methodology. The species composition of weed vegetation was established using the Agroecological Atlas of Russia. Conventional insect accounting methods were used to consider phytophages and plants damaged by them. It was established that the dominant pests of winter grains in the early periods of vegetation include phytophage bugs and cereal leaf beetle Oulema melanopus L. The maximum number of phytophage bugs was noted during the earing-flowering period. The proportion of pathogens of spike Fusarium head blight, tan spot and Septoria spot prevailed in the pathogenic complex. In total, 36.7% of varieties, including Kuma, Deya, Esaul, Starshina, Delta, Nota and Antonina, were medium-resistant to Fusarium head blight damage. According to the results of a comprehensive assessment of winter wheat selection by the parameters of adaptability, productivity and resistance to diseases, highly plastic genotypes responsive to environmental factors were distinguished: Kuma, Deya, Veda, Tanya, and List 25 (bi = 2.2; 2,3; 2,1). The selection of varieties was based on the adaptive properties of winter wheat. Fusarium head blight decreased by 20.8% in the mixed variety Batko+Deya, and the yield of mixed variety crops increased by 9%. Methods for compiling mixed varieties and designing the mosaics of varieties in the area of grain agrocenosis are recommended. Keywords: winter wheat, sustainability of agrocenosis, productivity, ecological plasticity, mixed varieties

scholarly journals Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153642 ◽  
Author(s):  
Miroslava Cuperlovic-Culf ◽  
Lipu Wang ◽  
Lily Forseille ◽  
Kerry Boyle ◽  
Nadine Merkley ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Head Blight ◽  
Wheat Varieties

scholarly journals Effect of Seeding Rate and Seed Treatment Fungicides on Agronomic Performance, Fusarium Head Blight Symptoms, and DON Accumulation in Two Winter Wheats

Plant Disease ◽  
2005 ◽  
Vol 89 (10) ◽  
pp. 1109-1113 ◽  
Author(s):  
A. W. Schaafsma ◽  
L. Tamburic-Ilincic
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Seed Treatment ◽  
Flowering Period ◽  
Head Blight ◽  
Seeding Rate ◽  
Wheat Varieties ◽  
Yield And Quality ◽  
Plant Emergence ◽  
Foliar Fungicide

Fusarium head blight (FHB), caused by Fusarium graminearum, is an important disease of wheat (Triticum aestivum). FHB reduces yield and quality, and the pathogen produces several toxins in the grain, the most important being deoxynivalenol (DON). In North America, the foliar fungicide tebuconazole is used to reduce FHB symptoms and DON accumulation. Because of the narrow window required for its application, uniform flowering of wheat is important. The objective of this study was to investigate the influence of variety, seeding rate, and seed treatment fungicides on the flowering period of winter wheat and their effect on FHB symptoms and DON accumulation. The seed of two winter wheat varieties (Pioneer 25W60 and Pioneer 25R57) was treated with Dividend XL (difeconazole+metalaxyl), Vitaflo 280 (thiram+carbathiin), Raxil (tebuconazole), and Baytan 30 (triadimenol) and planted at 320, 480, and 640 seeds per m2 for each treatment at Ridgetown, ON, Canada in 2000 and 2001. The plots were sprayed with tebuconazole at 50% anthesis and inoculated with F. graminearum 3 days later. Increased seeding rate increased the number of emerged plants, tillers, spikes per m2, and yield. All seed treatments, compared to nontreated controls, increased plant emergence and number of spikes per m2, and all except tebuconazole increased tillering and yield. Increased seeding rate decreased the length of flowering period. As flowering period increased, FHB index and DON level decreased, suggesting that greater infection was linked to more uniform flowering.

scholarly journals Role of Fungicides, Application of Nozzle Types, and the Resistance Level of Wheat Varieties in the Control of Fusarium Head Blight and Deoxynivalenol

Toxins ◽  
2011 ◽  
Vol 3 (11) ◽  
pp. 1453-1483 ◽  
Author(s):  
Ákos Mesterházy ◽  
Beáta Tóth ◽  
Monika Varga ◽  
Tibor Bartók ◽  
Ágnes Szabó-Hevér ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Head Blight ◽  
Resistance Level ◽  
Wheat Varieties

scholarly journals Resistance of winter wheat varieties registered in the Czech Republic to Fusarium head blight in relation to the presence of specific Rht Alleles

2010 ◽  
Vol 46 (No. 3) ◽  
pp. 122-134 ◽  
Author(s):  
J. Chrpová ◽  
V. Šíp ◽  
L. Štočková ◽  
Z. Milec ◽  
L. Bobková
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Grain Weight ◽  
The Other ◽  
Head Blight ◽  
Wheat Varieties ◽  
Frequency Distributions ◽  
Infection Period ◽  
Symptom Scores ◽  
Winter Wheat Varieties

Resistance of 31 winter wheat varieties (bred in 6 European countries) to Fusarium head blight (FHB) was evaluated in field trials lasting for three years (2007, 2008, 2009) after artificial inoculation with Fusarium culmorum. The data on deoxynivalenol (DON) content were supplemented by symptom scores and determination of % of Fusarium damaged grains and % reductions of thousand-grain weight and grain weight per spike due to infection. These varieties and also 120 advanced breeding lines from the Úhřetice Breeding Station, SELGEN company were examined for the presence of gibberellic acid (GA) insensitive semi-dwarfing genes to evaluate their effect on FHB. The conditions of experimental years highly influenced the performance of all characters. The highest production of DON occurred in 2008 at a high temperature and high moisture content during the infection period. In all years the moderate resistance to accumulation of DON (at the level of Swiss variety Arina) was detected in the Czech varieties Bakfis, Federer, Baletka, Samanta and Sakura. Among these varieties, Federer showed a low accumulation of DON at a relatively higher symptom expression and greater reduction of grain weight per spike, but the other four varieties and the reference variety Arina expressed resistance in all the examined traits. The varieties Pitbull, Cubus, Kodex and Bagou were found to be highly susceptible to FHB. The presence of the dwarfing allele Rht-D1b resulted in a significantly higher mean symptom score and also in a higher affection of the other traits. Nonetheless, the analysis of frequency distributions in symptom scores showed the presence of resistant lines also among the GA insensitive lines, but with a lower frequency than in the group of GA sensitive genotypes. A relatively greater effect on manifestation of the disease had plant height, and therefore it is suggested that the adverse impact of Rht-D1b on FHB resistance could be to a high degree excluded by opting for taller Rht-D1b genotypes.

scholarly journals Fusarium Head Blight: Effect of Infection Timing on Spread of Fusarium graminearum and Spatial Distribution of Deoxynivalenol within Wheat Spikes

2020 ◽  
Vol 9 (1) ◽  
pp. 79
Author(s):  
Elias Alisaac ◽  
Anna Rathgeb ◽  
Petr Karlovsky ◽  
Anne-Katrin Mahlein
Keyword(s):  
Spatial Distribution ◽  
Fusarium Head Blight ◽  
Fusarium Graminearum ◽  
Fungal Biomass ◽  
Head Blight ◽  
Wheat Varieties ◽  
Fungal Dna ◽  
Infection Timing ◽  
Wheat Kernels ◽  
Days After Anthesis

Most studies of Fusarium head blight (FHB) focused on wheat infection at anthesis. Less is known about infections at later stages. In this study, the effect of infection timing on the development of FHB and the distribution of fungal biomass and deoxynivalenol (DON) along wheat spikes was investigated. Under greenhouse conditions, two wheat varieties were point-inoculated with Fusarium graminearum starting from anthesis until 25 days after anthesis. The fungus and fungal DNA were isolated from the centers and the bases of all the spikes but not from the tips for all inoculation times and both varieties. In each variety, the amount of fungal DNA and the content of DON and deoxynivalenol-3-glucoside (DON-3-G) were higher in the center than in the base for all inoculation times. A positive correlation was found between the content of fungal DNA and DON in the centers as well as the bases of both varieties. This study showed that F. graminearum grows downward within infected wheat spikes and that the accumulation of DON is largely confined to the colonized tissue. Moreover, F. graminearum was able to infect wheat kernels and cause contamination with mycotoxins even when inoculated 25 days after anthesis.

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