scholarly journals Bioconversion of Lignocellulosic Biomass into Value Added Products under Anaerobic Conditions: Insight into Proteomic Studies

2021 ◽  
Vol 22 (22) ◽  
pp. 12249
Author(s):  
Martha Inés Vélez-Mercado ◽  
Alicia Guadalupe Talavera-Caro ◽  
Karla María Escobedo-Uribe ◽  
Salvador Sánchez-Muñoz ◽  
Miriam Paulina Luévanos-Escareño ◽  
...  

Production of biofuels and other value-added products from lignocellulose breakdown requires the coordinated metabolic activity of varied microorganisms. The increasing global demand for biofuels encourages the development and optimization of production strategies. Optimization in turn requires a thorough understanding of the microbial mechanisms and metabolic pathways behind the formation of each product of interest. Hydrolysis of lignocellulosic biomass is a bottleneck in its industrial use and often affects yield efficiency. The accessibility of the biomass to the microorganisms is the key to the release of sugars that are then taken up as substrates and subsequently transformed into the desired products. While the effects of different metabolic intermediates in the overall production of biofuel and other relevant products have been studied, the role of proteins and their activity under anaerobic conditions has not been widely explored. Shifts in enzyme production may inform the state of the microorganisms involved; thus, acquiring insights into the protein production and enzyme activity could be an effective resource to optimize production strategies. The application of proteomic analysis is currently a promising strategy in this area. This review deals on the aspects of enzymes and proteomics of bioprocesses of biofuels production using lignocellulosic biomass as substrate.

2022 ◽  
Vol 9 (1) ◽  
pp. 17
Author(s):  
Malinee Sriariyanun ◽  
Nichaphat Kitiborwornkul ◽  
Prapakorn Tantayotai ◽  
Kittipong Rattanaporn ◽  
Pau-Loke Show

Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc], which showed that their activities retained at more than 95%. However, [Ch][OAc] had toxicity to CBD2 and CBD3 cultures, in which only 32.85% and 12.88% were alive at 0.5 M [Ch][OAc]. Based on the specific enzyme activities, the sugar amounts produced from one-pot processes using 1 mg of CBD2 and CBD3 were higher than that of Celluclast 1.5 L by 2.0 and 4.5 times, respectively, suggesting their potential for further application in the biorefining process of value-added products.


2019 ◽  
Vol 158 ◽  
pp. 918-925 ◽  
Author(s):  
Chengyu Dong ◽  
Ying Wang ◽  
Huaimin Wang ◽  
Carol Sze Ki Lin ◽  
Hsien-Yi Hsu ◽  
...  

2016 ◽  
Vol 70 (4) ◽  
Author(s):  
Mónica L. Chávez-González ◽  
Lluvia I. López-López ◽  
Raúl Rodríguez-Herrera ◽  
Juan C. Contreras-Esquivel ◽  
Cristóbal N. Aguilar

AbstractCitrus peels are underutilized in the processing industry and are commonly considered as waste as their chemical composition varies. However, it is possible to recover some value-added products, one of them being essential oil, which is widely used in the food industry. Due to the current increase in global demand for citrus essential oils, it is necessary to implement processes directed to the maximum recovery of oil from waste peels. Numerous efforts have been made without achieving significant improvements in yields. In this work, enzymatic pretreatments of orange, lemon, and grapefruit peels were applied to achieve increased yields of essential oil extracted by hydro-distillation. In two of the sources it was possible to increase the essential oil yield from two to six times. Moreover, a significant amount of fermentable sugars which can be used in the production of certain other compounds of interest through fermentation was obtained.


Author(s):  
Nicole Marasca ◽  
Isabella Cardoso ◽  
Magale Rambo ◽  
Daniel Bertuol ◽  
Michele Rambo ◽  
...  

Cupuaçu husk (CH) is the waste of a common fruit from a native species of the Brazilian Legal Amazon. The current study investigated the influence of ultrasound (US) combined with aqueous, acid, alkaline, and ionic liquid (IL) pretreatments on the chemical and physical aspects of CH and the yield of chemical platforms production, 5-hydroxymethylfurfural (HMF) and furfural (FF), using IL. Scanning electron microscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy were used to feature the raw and pretreated biomass. The highest levels of glucose (9.90 g L-1) were observed in the liquid fraction resulting from the acid + US pretreatment followed by acid hydrolysis. The IL + US pretreatment recorded the best performance in removing lignin. Based on XRD analyses, ultrasound increased crystallinity of all pretreated samples as a result of the removal of cellulose’s amorphous fraction. However, it promoted accessibility to adopted reagents by increasing biomass exposure due to cavitation. The best yields of HMF and FF were recorded from hydrolysis of the solid fraction resulting from the acid + US (12.94%) and alkaline + US (48.84%) pretreatment, respectively. These results indicate satisfactory performance of ultrasound assisted pretreatments to the simplified and economic conversion of biomass into value-added products.


Author(s):  
Paul Harris

New Zealand is currently debating its potential to contribute to what is commonly known as the "knowledge economy". In discussing the knowledge economy, politicians and certain academics tend to reduce it to industries such as information technology and biotechnology. However, both skill and design are forms of knowledge that are incorporated into value-added products such as high quality furniture. Denmark and Italy are two countries that have taken the lead in the export of such products.This paper explores the role of skill and design in the Italian and Danish furniture industry in comparison with developments in the NZ furniture industry.


Fuel ◽  
2021 ◽  
pp. 122678
Author(s):  
G. Velvizhi ◽  
Chandamita Goswami ◽  
Nagaraj P. Shetti ◽  
Ejaz Ahmad ◽  
Kamal Kishore Pant ◽  
...  

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