Ly6c as a New Marker of Mouse Blood Vessels: Qualitative and Quantitative Analyses on Intact and Ischemic Retinas

Ly6c is an antigen commonly used to differentiate between classical and non-classical monocytes/macrophages. Here we show its potential as a marker of the mouse vasculature, particularly of the retinal vascular plexuses. Ly6c was immunodetected in several tissues of C57BL/6 mice using isolectin IB4 as the control of vasculature staining. In the retina, Ly6c expression was analyzed qualitatively and quantitatively in intact, ischemic, and contralateral retinas from 0 to 30 days after the insult. Ly6c expression was observed in all organs and tissues tested, with a brighter signal and more homogeneous staining than the IB4. In the retinas, Ly6c was well expressed, allowing a detailed study of their anatomy. The three retinal plexuses were morphologically different, and from the superficial to the deep one occupied 15 ± 2, 24 ± 7, and 38 ± 1.4 percent of the retinal surface, respectively. In the injured retinas, there was extravasation of the classically activated monocyte/macrophages (Ly6chigh) and the formation of new vessels in the superficial plexus, increasing the area occupied by it to 25 ± 1%. In the contralateral retinas, the superficial plexus area decreased gradually, reaching significance at 30 days, and Ly6c expression progressively disappeared in the intermediate and deep plexuses. Although the role of Ly6c in vascular endothelial cell function is still not completely understood, we demonstrate here that Ly6c can be used as a new specific marker of the mouse vasculature and to assess, qualitatively and quantitatively, vascular changes in health and disease.

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Enhanced Vascular Endothelial Cell Function on Nanostructured Titanium Surface Features: The Role of Nano to Submicron Roughness

MRS Proceedings ◽

10.1557/proc-1136-dd04-04 ◽

2008 ◽

Vol 1136 ◽

Cited By ~ 2

Author(s):

Jing Lu ◽

Dongwoo Khang ◽

Thomas J. Webster

Keyword(s):

Cell Adhesion ◽

Endothelial Cell ◽

Cell Function ◽

Vascular Endothelial Cell ◽

Vascular Endothelial ◽

Endothelial Cell Function ◽

Titanium Surfaces ◽

Endothelial Cell Adhesion

ABSTRACTTo study the contribution of different surface feature properties in improving vascular endothelial cell adhesion, rationally designed nano/sub-micron patterns with various dimensions were created on titanium surfaces in this study. In vitro results indicated that endothelial cell adhesion was improved when the titanium pattern dimensions decreased into the nano-scale. Specifically, endothelial cells preferred to adhere on sub-micron and nano rough titanium substrates compared to flat titanium. Moreover, titanium with nano and sub-micron roughness and with the same chemistry as compared to flat titanium, had significantly greater surface energy. Thus, the present study indicated the strong potential of surface nanotopography and nano/sub-micron roughness for improving current vascular stent design.

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MAINTENANCE OF TISSUE PERFUSION IN PROESTRUS FEMALE BUT NOT IN MALE ANIMALS AFTER HEMORRHAGE AND RESUSCITATION: THE ROLE OF VASCULAR ENDOTHELIAL CELL FUNCTION.

Shock ◽

10.1097/00024382-200206001-00161 ◽

2002 ◽

Vol 17 (Supplement) ◽

pp. 53-54

Author(s):

Z. F. Ba ◽

P. Wang ◽

L. W. Rue ◽

K. I. Bland ◽

I. H. Chaudry

Keyword(s):

Endothelial Cell ◽

Cell Function ◽

Vascular Endothelial Cell ◽

Tissue Perfusion ◽

Vascular Endothelial ◽

Endothelial Cell Function

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THE MECHANISM OF BENEFICIAL EFFECTS OF ADRENOMEDULLIN (AM) AND ADRENOMEDULLIN BINDING PROTEIN-1 (AMBP-1) IN SEPSIS: MAINTENANCE OF VASCULAR ENDOTHELIAL CELL FUNCTION.

Shock ◽

10.1097/00024382-200206001-00156 ◽

2002 ◽

Vol 17 (Supplement) ◽

pp. 52 ◽

Cited By ~ 2

Author(s):

P. Wang ◽

D. E. Fowler ◽

S. Yang ◽

D. A. Ornan ◽

M. Zhou

Keyword(s):

Endothelial Cell ◽

Cell Function ◽

Binding Protein ◽

Vascular Endothelial Cell ◽

Vascular Endothelial ◽

Endothelial Cell Function ◽

Beneficial Effects

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Peroxynitrite increases iNOS through NF-κB and decreases prostacyclin synthase in endothelial cells

AJP Cell Physiology ◽

10.1152/ajpcell.00295.2001 ◽

2002 ◽

Vol 282 (2) ◽

pp. C395-C402 ◽

Cited By ~ 132

Author(s):

Christy-Lynn M. Cooke ◽

Sandra T. Davidge

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Vascular Reactivity ◽

Cell Function ◽

Vascular Endothelial Cell ◽

Vascular Endothelial ◽

Cell Nuclei ◽

Endothelial Cell Function ◽

Protein Mass ◽

Prostacyclin Synthase

Peroxynitrite, a marker of oxidative stress, is elevated in conditions associated with vascular endothelial cell dysfunction, such as atherosclerosis, preeclampsia, and diabetes. However, the effects of peroxynitrite on endothelial cell function are not clear. The endothelium-derived enzymes nitric oxide synthase (NOS) and prostaglandin H synthase (PGHS) mediate vascular reactivity and contain oxidant-sensitive isoforms (iNOS and PGHS-2) that can be induced by nuclear factor (NF)-κB activation. We investigated the effect(s) of peroxynitrite on NOS and PGHS pathways in endothelial cells. We hypothesized that peroxynitrite will increase levels of iNOS and PGHS-2 through activation of NF-κB. Western immunoblots of endothelial cells show that 3-morpholinosydnonimine (SIN-1; 0.5 mM), a peroxynitrite donor, increased iNOS protein mass, which can be inhibited by pyrroline dithiocarbamate (an NF-κB inhibitor) (167 ± 24.2 vs. 78 ± 19%, P < 0.05, n = 6). SIN-1 treatment also significantly increased NF-κB translocation into endothelial cell nuclei (135 ± 10%, P < 0.05). Endothelial NOS, PGHS-1, and PGHS-2 protein levels were not altered by SIN-1. However, prostacyclin synthase protein mass, but not mRNA, was significantly reduced in SIN-1-treated endothelial cells (78 ± 8.9%, P < 0.05). Our results illustrate novel mechanisms through which peroxynitrite may modulate vascular endothelial function.

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Vitamin D Promotes Vascular Endothelial Cell Function via the Regulation of miR-199a-5p

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2019.2186 ◽

2019 ◽

Vol 9 (12) ◽

pp. 1662-1669

Author(s):

Lianman He ◽

Yong Wang ◽

Min Liu ◽

Ling Li

Keyword(s):

Vitamin D ◽

Endothelial Cell ◽

Cell Function ◽

Vascular Endothelial Cell ◽

Cell Counting ◽

Biological Behavior ◽

Luciferase Reporter ◽

Vascular Endothelial ◽

Endothelial Cell Function ◽

The Impact

Essential hypertension (EH) is a main risk factor for cardiovascular disease. Vitamin D (VD) levels are inversely related to hypertension. MicroRNAs (miRNA or miR) are involved in various diseases, including EH. Till now, the role of miR-199a-5p in EH remains unclear. Cell counting kit-8, flow cytometry and Transwell assay were carried out in the current study to study the effects of VD on the biological behavior of Human umbilical vein endothelial cells (HUVECs). The expression of miR-199a-5p was subsequently determined using reverse transcription-quantitative (RT-q) PCR. TargetScan prediction and double luciferase reporter gene detection were applied to confirm the binding sites between Sirtuin 1 (SIRT1) and miR-199a-5p. The results showed that VD promoted the proliferation and migration of HUVECs and reduced cell apoptosis. VD was observed to significantly reduced miR-199a-5p level in HUVECs. Transfection of the miR-199a-5p mimic was indicated to reverse the influence of VD on the proliferation, migration and apoptosis of HUVECs. SIRT1 was also confirmed to be a target gene of miR-199a-5p. Western blot analysis and RT-qPCR were performed to measure the impact of VD on the SIRT1/AMP-activated protein kinase (AMPK)- /NFB pathway. The results demonstrated that VD increased SIRT1 expression and p-AMPK- and decreased the expression of p-p65, and the transfection of miR-199a-5p mimic reversed these effects. In conclusion, the results of the current study indicated that VD may relieve EH through promoting vascular endothelial cell function via regulating miR-199a-5p.

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