scholarly journals New Insight into the Systematics of European Lepidocyrtus (Collembola: Entomobryidae) Using Molecular and Morphological Data

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 302
Author(s):  
Daniel Winkler ◽  
Eduardo Mateos ◽  
György Traser ◽  
Ferenc Lakatos ◽  
Viktória Tóth
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Analysis ◽  
Morphological Characters ◽  
Morphological Data ◽  
Gene Sequences ◽  
European Species ◽  
Individual Gene ◽  
Representative Species ◽  
Species Groups ◽  
Insight Into

The Collembolan genus Lepidocyrtus is subdivided into up to eight subgenera, of which only Lepidocyrtus s.str. (Bourlet, 1839) and Lanocyrtus (Yoshii & Suhardjono, 1989) are represented by European species. The discovery of unique characters in the European species Lepidocyrtus tomosvaryi (rounded dental tubercle) and L. peisonis (lateral tuft of long filiform chaetae in abdomen III) has only described so far for species of the subgenera Setogaster (Salmon, 1951) and Cinctocyrtus (Yoshii & Suhardjono, 1989) and has raised the need to perform a molecular analysis by involving other representative species of the genus. For this study, phylogenetic analysis of 15 Lepidocyrtus species occurring in the Carpathian Basin were carried out. The analyses, which was based on both concatenated datasets of COII and EF1-α sequences and individual gene sequences, clearly placed L. tomosvaryi within the subgenus Lanocyrtus and L. peisonis within Lepidocyrtus s.srt. European species groups defined on the basis of morphological characters were only partly confirmed by the concatenated and COII analyses because of the splitting of the pallidus–serbicus-group, whereas EF1- α sequences weakly supported this group.

Untangling Lepidocyrtus (Collembola, Entomobryidae): new molecular data shed light on the relationships of the European groups

2018 ◽  
Vol 32 (3) ◽  
pp. 639 ◽  
Author(s):  
Eduardo Mateos ◽  
Paula Escuer ◽  
Galina Buşmachiu ◽  
Marta Riutort ◽  
Marta Álvarez-Presas
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Gene Sequences ◽  
European Species ◽  
Species Groups ◽  
A New Species ◽  
Shed Light

European Lepidocyrtus species are usually grouped into five species-groups within two subgenera, Lepidocyrtus s.s. and Lanocyrtus, defined by the distribution of scale covering and dorsal head and body macrochaetotaxy. The discovery of several Lepidocyrtus populations with morphological characters intermediate between two species-groups suggested the need for the present study to test whether molecular data provide support to these groups. The morphology and gene sequences of 110 specimens belonging to 19 species of European Lepidocyrtus have been studied. Our molecular results are congruent with the distribution of the European species into the five groups established in the literature on the basis of morphological characters, but also indicated that subgenus Lanocyrtus is paraphyletic while subgenus Lepidocyrtus s.s. is monophyletic. A new species, Lepidocyrtus intermedius, is described, and a redefinition of European species-groups is proposed based on chaetotaxy.

scholarly journals Hypotheses from mitochondrial DNA: congruence and conflict between DNA sequences and morphology in Dolichopodinae systematics (Diptera:Dolichopodidae)

2010 ◽  
Vol 24 (1) ◽  
pp. 32 ◽  
Author(s):  
Marc Pollet ◽  
Christoph Germann ◽  
Samuel Tanner ◽  
Marco Valerio Bernasconi
Keyword(s):  
Phylogenetic Analysis ◽  
Mitochondrial Dna ◽  
Molecular Analysis ◽  
Dna Sequences ◽  
Morphological Characters ◽  
Species Group ◽  
Data Matrix ◽  
Cyt B ◽  
Molecular Phylogenetic ◽  
Species Groups

The molecular phylogeny of the subfamily Dolichopodinae (Diptera : Dolichopodidae) is reconstructed based on 79 species of 7 dolichopodine genera as ingroup, and 10 non-dolichopodine species from different genera as outgroup. A Bayesian analysis based on a mitochondrial DNA dataset consisting of 1702 characters (COI : 810; 12S : 366; 16S : 526) was carried out. Genital and non-genital morphological characters from a hitherto unpublished data matrix (based on 57 Dolichopodidae species) were used to explain and support the lineages hypothesised by our molecular phylogenetic analysis. The monophyly of the subfamily Dolichopodinae, and of the genera Dolichopus and Gymnopternus, was confirmed. The molecular analysis yielded nine species groups in Dolichopus that were proposed in previous studies using COI and Cyt-b. No evidence was found to support a clade including Dolichopus, Ethiromyia, and Gymnopternus. The genus Hercostomus proved polyphyletic with respect to Poecilobothrus, Sybistroma, and Gymnopternus. The following lineages were represented by strongly supported clades: Hercostomus germanus species group, H. vivax species group, H. nigrilamellatus species group, H. plagiatus species group, H. longiventris species group, H. fulvicaudis species group, and Poecilobothrus, Gymnopternus, Tachytrechus and Sybistroma (including Hercostomus nanus and H. parvilamellatus). Two clades that were previously established on the basis of morphology were confirmed in our phylogenetic analysis: (i) Poecilobothrus and the flower-feeding Hercostomus germanus species group, and (ii) the H. longiventris lineage and Sybistroma. In most cases, the groups identified in the molecular analysis could be supported and explained by morphological characters. Species of the Hercostomus germanus species group, Poecilobothrus, the Hercostomus longiventris species group, and a Sybistroma subclade have a similar microhabitat affinity.

A phylogenetic analysis of the dung beetle genus Phanaeus (Coleoptera: Scarabaeidae) based on morphological data

2007 ◽  
Vol 38 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Dana Price
Keyword(s):  
Phylogenetic Analysis ◽  
Dung Beetle ◽  
Morphological Characters ◽  
Western Hemisphere ◽  
Morphological Data ◽  
Sister Taxon ◽  
Parsimony Analysis ◽  
Species Groups ◽  
Equal Weighting

The genus Phanaeus (Scarabaeidae: Scarabaeinae) forms an important part of the dung beetle fauna in much of the Western Hemisphere. Here a phylogeny for Phanaeus , including 49 Phanaeus sp., and 12 outgroup taxa, is proposed. Parsimony analysis of 67 morphological characters, and one biogeographical character produced 629 equally parsimonious trees of 276 steps. Oxysternon , the putative sister taxon is nested well within the subgenus Notiophanaeus , implying that Oxysternon might ultimately need to be synonymized with Phanaeus . Species groups of Edmonds (1994) recovered as monophyletic are paleano , endymion, chalcomelas , tridens , triangularis , and quadridens . An 'unscaled' equal weighting analysis yielded 57,149 equally parsimonious trees of 372 steps. The strict consensus of these trees yielded a monophyletic Phanaeus with the inclusion of Oxysternon . Bootstrap values are relatively low and some clades are unresolved.

scholarly journals Phylogenetic relationships based on morphological data and taxonomy of the genus Salvadora Baird & Girard, 1853 (Reptilia, Colubridae)

2021 ◽  
Vol 764 ◽  
pp. 85-118
Author(s):  
Carlos A. Hernández-Jiménez ◽  
Oscar Flores-Villela ◽  
Aranzazú Aguilar-Bremauntz ◽  
Jonathan A. Campbell
Keyword(s):  
Phylogenetic Analysis ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Morphological Data ◽  
Phylogenetic Hypothesis ◽  
Distribution Maps ◽  
Qualitative And Quantitative ◽  
Species Groups ◽  
Parsimony Criterion ◽  
Entire Distribution

The genus Salvadora has not been subjected to a modern phylogenetic analysis. Described in 1853, its taxonomic history is complex and confusing. In this study, we evaluate the monophyly of the genus and present the first phylogenetic hypothesis based on an analysis of 66 qualitative and quantitative morphological characters of over 1000 specimens representing all described taxa across their entire distribution. Morphological characters were processed in Fast Morphology for subsequent phylogenetic analysis in PAUP under the maximum parsimony criterion. We obtained a single tree in which Salvadora appears as a monophyletic group with two clearly defined geographic species groups: a southern mexicana group and a northern grahamiae group. Based on our phylogenetic hypothesis, we evaluate the taxonomic status of all described taxa. Additionally, we include a diagnosis for all species, distribution maps, and an illustrated dichotomous taxonomic key of the genus.

A revised infrageneric classification of Bellevalia Lapeyr. (Asparagaceae: Scilloideae) based on molecular analysis

Phytotaxa ◽  
2021 ◽  
Vol 525 (1) ◽  
pp. 70-84
Author(s):  
AZARNOOSH JAFARI ◽  
JAMIL VAEZI ◽  
MOHAMMAD MAHDI FORGHANIFARD ◽  
FÉLIX FOREST ◽  
JOHN C. MANNING
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Analysis ◽  
Trnl Intron ◽  
Leaf Margin ◽  
Infrageneric Classification ◽  
Flower Shape ◽  
Representative Species

Within the genus Bellevalia (Asparagaceae: Scilloideae: Hyacintheae), flower shape and colour, the ratio of leaf to scape length, and the orientation of the pedicels were traditionally used to delimit sections, subsections and species. We conducted a phylogenetic analysis of representative species from sections Bellevalia, Conicae, Nutantes and Oxydontae, representing four out of the six sections that are currently recognised, using the four chloroplast regions rbcL, matK, trnL intron, and trnL-F spacer. Our results indicate that the sections are not monophyletic as currently circumscribed. Our analyses retrieve two major, well-supported clades. The first clade (sect. Conicae) includes only species with ciliate leaves and green-veined perianth lobes, and the second clade (sect. Bellevalia) includes the species lacking these characteristics. Within the second clade, the species with yellow anthers are separated from those with violet anthers. Our molecular analysis does not support the traditional subdivisions of the genus but establishes the value of cilia on the leaf margin, green veins in the perianths lobes, and the colour of the anthers as indicators of relationships among the species. We recommend that the current four sections and six subsections of Bellevalia be reduced to two sections without subsections and we provide an updated and corrected nomenclature for these sections and subsections, designating lectotypes where necessary.

Combined molecular and morphological phylogeny of Ephemerellinae (Ephemerellidae: Ephemeroptera), with remarks about classification

Zootaxa ◽  
2009 ◽  
Vol 1991 (1) ◽  
pp. 28-42 ◽  
Author(s):  
T. HEATH OGDEN ◽  
JONATHAN T. OSBORNE ◽  
LUKE M. JACOBUS ◽  
MICHAEL F. WHITING
Keyword(s):  
Morphological Analysis ◽  
Sequence Data ◽  
Histone H3 ◽  
Morphological Characters ◽  
Morphological Data ◽  
Rdna Genes ◽  
Dna Sequence Data ◽  
Extant Genus ◽  
Species Groups ◽  
Combined Data

This study represents the first combined molecular and morphological analysis for the mayfly family Ephemerellidae (Ephemeroptera), with a focus on the relationships of genera and species groups of the subfamily Ephemerellinae. The phylogeny was constructed based on DNA sequence data from 3 nuclear (18S rDNA, 28S rDNA, histone H3) and 2 mitochondrial (12S rDNA, 16S rDNA) genes, and 23 morphological characters. Taxon sampling for Ephemerellidae included exemplars from all 25 extant genus groups and additional representatives from those genera with the highest diversity. Ephemerellidae appears to consist of three major clades. Ephemerella, the largest genus of Ephemerellidae, and Serratella were not supported as monophyletic, and each had representatives in two of the three major clades. However, the genera Drunella and Cincticostella were supported as monophyletic. Lineages strongly supported as monophyletic include a grouping of the Timpanoginae genera Timpanoga, Dannella, Dentatella and Eurylophella, and groupings of the Ephemerellinae genera Torleya, Hyrtanella and Crinitella and the genera Kangella, Uracanthella and Teloganopsis. The placement of the Timpanoginae genus Attenella fell within Ephemerellinae, based on molecular and combined data, but it grouped with other Timpanoginae based on morphological data alone. Further study and analysis of Ephemerellidae morphology is needed, and classification should be revised, if it is to reflect phylogenetic relationships.

scholarly journals Phylogeny of Libellula Linnaeus (Odonata: Insecta)

Zootaxa ◽  
2002 ◽  
Vol 87 (1) ◽  
pp. 1 ◽  
Author(s):  
FRANK LOUIS CARLE ◽  
KARL M. KJER
Keyword(s):  
Phylogenetic Analysis ◽  
Type Species ◽  
Sister Group ◽  
Morphological Characters ◽  
Sensu Stricto ◽  
Monophyletic Group ◽  
Representative Species

Phylogenetic analysis was performed on a set of 242 morphological characters. The taxon sample included 31 Libellula, and representative species from selected libeluline tribes, from all libellulid subfamilies, from all libelluloid families, from all anisopteran superfamilies, and Epiophlebia. Corduliinae was shown to be paraphyletic even among genera characterized by a well developed anal loop bisector. Sympetrini was found to be polyphyletic with Crocothemis the sister group to Libellulini. The traditional placement of Trameini, far from Libellulini is in doubt, because it is here placed as the sister group to Crocothemis + Libellulini. Kennedy’s phylogeny of Libellula was largely corroborated, with the following exceptions: the subgenera Libellula, Eolibellula, and Syntetrum form a monophyletic group which is the sister group to a clade including Belonia, Holotania, Neotetrum, and Eotainia subgenus nov. [type species Mesothemis composita Hagen]; and Eurothemis is determined to be the sister group of Ladona instead of Neotetrum. In addition we confirm Belonia to be monophyletic, and find Platetrum + Plathemis to form a monophyletic group, sister to Ladona + Eurothemis; these four subgenera together form the sister group to Libellula sensu stricto (s.s.).

Phylogenetic analysis of the Neotropical Pristimantis leptolophus species group (Anura: Craugastoridae): molecular approach and description of a new polymorphic species 

Zootaxa ◽  
2017 ◽  
Vol 4242 (2) ◽  
pp. 313
Author(s):  
MAURICIO RIVERA-CORREA ◽  
CARLOS JIMÉNEZ-RIVILLAS ◽  
JUAN M. DAZA
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Reconstruction ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Species Group ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
Molecular Evidence ◽  
Polymorphic Species ◽  
Species Groups

Pristimantis, distributed throughout the New World tropics, is the most speciose vertebrate genus. Pristimantis presents an enormous morphological diversity and is currently divided into several demonstrably non-monophyletic phenetic species groups. With the purpose of increasing our understanding of Pristimantis systematics, we present the first phylogenetic analysis using molecular evidence to test the monophyly and infer evolutionary relationships within the Pristimantis leptolophus group, an endemic group of frogs from the highlands of the Colombian Andes. Our phylogenetic reconstruction recovers the group as monophyletic with high support, indicating general concordance between molecular data and morphological data. In addition, we describe a new polymorphic species lacking conspicuous tubercles, a regular attribute among species of the P. leptolophus species group and endemic from the Páramo de Sonsón complex (Antioquia, Colombia). The phylogenetic position of the new species is inferred and other systematic implications in the light of our results are discussed. 

Characterisation of the Australian Nacophorini using adult morphology, and phylogeny of the Geometridae based on morphological characters

Zootaxa ◽  
2008 ◽  
Vol 1736 (1) ◽  
pp. 1 ◽  
Author(s):  
CATHERINE J. YOUNG
Keyword(s):  
Phylogenetic Analysis ◽  
Majority Rule ◽  
Morphological Character ◽  
Morphological Characters ◽  
Data Sources ◽  
Morphological Data ◽  
Bootstrap Support ◽  
Consensus Tree ◽  
Molecular Analyses ◽  
Character Set

The Australian Nacophorini and related taxa are described using a matrix of 116 adult morphological characters. Adults of 72 species are illustrated using photographs and electron micrographs. Subsets of the characters are used to conduct a phylogenetic analysis based on cladistic principles. The adult morphological character set was augmented with 17 characters from eggs and 27 from larvae. The resulting phylogeny is poorly resolved but provides support for many of the relationships recovered by previous molecular analyses of the group, including basal derivations for characters of Larentiinae and Sterrhinae relative to those of the rest of Geometridae, and the monophyly of the Geometrinae + Oenochrominae s. str. Combining 28S D2 datawith morphological data produced a matrix of 60 taxa and 590 characters. The majority rule consensus tree produced by the combined morphological and 28S D2 data is almost identical to the majority rule consensus tree produced by the 28S D2 data alone, except that bootstrap support is lower for most nodes. Common clades obtained from the molecular and morphological trees are described in terms of morphological data. On this basis a concept of the Australian Nacophorini includes Lithinini and Australian Archiearinae. Two robust groups within the tribe also are delimited using characters from all data sources. Comparsions are made between the Nearctic and Neotropical Nacophorini on the basis of shared morphological characters. Australian Boarmiini are defined by synapomorphies.

scholarly journals Molecular analysis of the N gene of canine distemper virus in dogs in Brazil

2007 ◽  
Vol 59 (3) ◽  
pp. 654-659 ◽  
Author(s):  
J.G. Castilho ◽  
P.E. Brandão ◽  
P. Carnieli Jr ◽  
R.N. Oliveira ◽  
C.I. Macedo ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Phylogenetic Analysis ◽  
Nervous System ◽  
Molecular Analysis ◽  
Canine Distemper Virus ◽  
Nucleotide Identity ◽  
N Gene ◽  
Canine Distemper ◽  
Gene Sequences ◽  
Rt Pcr

Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil.

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