Trueness of Intraoral Scanners in Implant-Supported Rehabilitations: An In Vitro Analysis on the Effect of Operators’ Experience and Implant Number

(1) Background: Intraoral scanners (IOS) are widely used in prosthodontics. However, a good trueness is mandatory to achieve optimal clinical results. The aim of the present in vitro study was to compare two IOS considering the operator’s experience and different implant clinical scenarios. (2) Methods: Two IOS (IT—Itero, Align Technology; and OS—Opera MC, Opera System, Monaco) were compared simulating three different clinical scenarios: single implant, two implants, and full-arch rehabilitation. Ten scans were taken for each configuration by two different operators (one expert, one inexperienced); influence of operator experience and the type of scanner used was investigated. (3) Results: Trueness of the scans differed between the experienced and non-experienced operator and this difference was statistically significant in all the three scenarios (p = 0.000–0.001, 0.037). A significant difference was present between the scanners (p = 0.000), in the two-implant and full-arch scenarios (p = 0.00). (4) Conclusions: Experience of the operator significantly affect trueness of IT and OP scanners. A statistically significant difference was present among IOS in the two-implant and full-arch scenarios.

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“Ormocer an innovative technology”: A replacement for conventional cements and veneer? A comparative in vitro analysis

European Journal of Dentistry ◽

10.4103/ejd.ejd_113_16 ◽

2017 ◽

Vol 11 (01) ◽

pp. 058-063 ◽

Cited By ~ 1

Author(s):

Vini Rajeev ◽

Rajeev Arunachalam ◽

Sanjna Nayar ◽

P. R. Arunima ◽

Sivadas Ganapathy ◽

...

Keyword(s):

Bond Strength ◽

Testing Machine ◽

In Vitro Study ◽

Sem Analysis ◽

Innovative Technology ◽

Group I ◽

Luting Cement ◽

Significant Difference ◽

Vitro Analysis

ABSTRACT Objective: This in vitro study was designed to assess shear bond strength (SBS) of ormocer flowable (OF) resin as a luting agent, ormocer as an indirect veneer material with portrayal of modes of failures using scanning electron microscope (SEM). Materials and Methods: Sixty maxillary central incisors were divided into Group I, II, and III with 20 samples each based on luting cement used. They were OF, self-adhesive (SA) cement, and total etch (TE) cement. These groups were subdivided into “a” and “b” of ten each based on the type of veneering materials used. Veneer discs were fabricated using Ormocer restorative (O) and pressable ceramic (C). Specimens were thermocycled and loaded under universal testing machine for SBS. The statistical analysis was done using one-way ANOVA post hoc Tukey honest significant difference method. Results: A significant difference was observed between the Groups I and II (P < 0.05). The highest mean bond strength when using ormocer veneer was obtained with the Group Ia (19.11 ± 1.92 Mpa) and lowest by Group IIa (8.1 ± 1.04 Mpa), whereas the highest mean bond strength while using ceramic veneer was of similar range for Group Ib (18.04 ± 4.08 Mpa) and Group IIIb (18.07 ± 1.40 Mpa). SEM analysis revealed OF and TE presented mixed type of failure when compared with SA where failure mode was totally adhesive. Conclusion: OF was found equally efficient like TE. Bond strength of ormocer as a veneer was not inferior to ceramic making it one of the promising additions in the field of dentistry.

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The Efficacy of Reciproc Blue® For Canal Cleanliness Following the Retreatment of Maxillary First Molar: In Vitro Analysis

Malaysian Journal of Science Health & Technology ◽

10.33102/mjosht.v7i1.142 ◽

2021 ◽

Vol 7 (1) ◽

pp. 22-27

Author(s):

Mohd Nazrin Isa ◽

Aws Hashim Ali Al-Kadhim ◽

Azlan Jaafar

Keyword(s):

Filling Material ◽

Volume Percentage ◽

Gutta Percha ◽

Maxillary Molars ◽

Significant Difference ◽

Time Required ◽

Vitro Analysis ◽

Cleaning And Disinfection ◽

In Vitro Analysis

Nonsurgical retreatment involves removing mechanical barriers such as gutta-percha to achieve proper cleaning and disinfection. The complexity of the anatomy of molar tooth gives challenge in retreatment procedure. Thus, this study evaluates the amount of residual gutta-percha after retreatment with rotary files (Reciproc Blue®) from each maxillary first molar canal using cone-beam computed tomography (CBCT) and the time required to accomplish it. Nine freshly extracted maxillary molars were instrumented and obturated. Preoperative CBCT was taken, and retreatment was done using Reciproc Blue®. CBCT was taken post retreatment, and the residual volume percentage of gutta-percha from each canal was calculated. The total retreatment time was recorded, and the data were statistically analyzed. The result shows no statistically significant difference in the amount of residual filling material in mesiobuccal, distobuccal, and palatal canal for maxillary first molar and total time used for retreatment with Reciproc Blue® system.

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Investigating the Cytotoxic and Anti-Bacterial Activity of Commercially Available Local Anesthetics: An In-Vitro Analysis

Journal of The Pakistan Dental Association ◽

10.25301/jpda.294.185 ◽

2020 ◽

Vol 29 (04) ◽

pp. 185-192

Author(s):

Eisha Imran ◽

◽

Faisal Moeen ◽

Humayoon Satti ◽

Lubna Rahman

Keyword(s):

Local Anesthetic ◽

Local Anesthetics ◽

Bacterial Activity ◽

Dilution Method ◽

Human Mscs ◽

Bacterial Strains ◽

Significant Difference ◽

Vitro Analysis ◽

In Vitro Analysis

OBJECTIVE: To evaluate and compare the influence of three local anesthetic dental formulations manufactured in France (Septodont), Korea (Medicaine) and Pakistan (HD-Caine) in terms of cytotoxicity and anti-bacterial activities. METHODOLOGY: 90 commercially available local anesthetic cartridges of similar composition (2% lidocaine with epinephrine 100,000) viz. Septodont, Medicaine and HD-Caine were randomly collected from three different Pakistani cities and were assigned as Group S, Group M and Group H, respectively. The cartridges were further divided into three sub-groups each consisting of 10 cartridges to first evaluate cytotoxicity on Mesenchymal Stem Cells (MSCs) using a flow cytometer and secondly to investigate anti-bacterial activity by measuring zones of inhibition and through Broth Dilution Method against five bacterial strains. RESULTS: The results indicated that Septodont (94.5±0.1) and Medicaine (94.7±0.0) showed the highest viability percentage with no significant difference when the two were compared (P=0.6). HD-Caine (93.9±0.0) showed the least, being significantly (P<0.01) different from Septodont and Medicane. A statistically significant (P<0.05) difference was identified between the three study groups regarding the anti-bacterial activity. HD-Caine showed the highest anti-bacterial potential, followed by Medicaine and Septodont. CONCLUSION: Mild toxicity was observed by all the three groups in human MSCs, justifying their safe use in clinical practice. Additionally, Medicane and HD-Caine showed significant anti-bacterial activity indicating their possible use as sterile irrigants. KEYWORDS: Dental anesthesia, Lidocaine, Epinephrine, Antibacterial activity HOW TO CITE: Imran E, Moeen F, Satti H, Rahman L. Investigating the cytotoxic and anti-bacterial activity of commercially available local anesthetics: An in-vitro analysis. J Pak Dent Assoc 2020;29(4):185-192.

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Toothpaste Composition Effect on Enamel Chromatic and Morphological Characteristics: In Vitro Analysis

Materials ◽

10.3390/ma12162610 ◽

2019 ◽

Vol 12 (16) ◽

pp. 2610 ◽

Cited By ~ 3

Author(s):

Alexandrina Muntean ◽

Sorina Sava ◽

Ada Gabriela Delean ◽

Ana Maria Mihailescu ◽

Laura Silaghi Dumitrescu ◽

...

Keyword(s):

Morphological Characteristics ◽

In Vitro Study ◽

Vitro Study ◽

Composition Effect ◽

Demineralized Enamel ◽

Colour Parameters ◽

Fixed Appliances ◽

Vitro Analysis ◽

In Vitro Analysis

The aim of this in vitro study was to assess the effect of toothpastes, with different compositions, on optical and morphological features of sound and demineralized enamel. We selected twenty-five teeth, recently extracted for orthodontic purposes, for this in vitro study. The teeth were caries free, without stains, fissures, filling or hypoplasia observed at inspection under standard conditions. Teeth were brushed (for 2–3 min, twice a day, for 21 days), with five different toothpastes (four commercially available and an experimental one) containing fluoride and hydroxyapatite. After that, teeth were demineralized with 37% orthophosforic acid (Ultra Etch®, Ultradent Products Inc., South Jordan, UT, USA) for 60 s. We repeated the brushing protocol for another 21 days on demineralized enamel. Enamel vestibular surfaces were examined using a spectrophotometer (Vita EasyShade -Vita Zahnfabrik, Bad Sackingen, Germany) and a Scanning Electron Microscope (Inspect S®, FEI Company, Hillsboro, OR, USA). Differences were statistically significant for colour parameters L* and ΔE*. SEM evaluation reveals demineralized enamel mineral gain after brushing with selected toothpastes. Toothpastes with specific ingredients can represent a balance between aesthetic and mineralization, and an oral hygiene correct algorithm is able to preserve enamel characteristics during ortodontic treatement with fixed appliances.

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In Vitro Analysis of the Erosive Potential of Whey Protein

Research Society and Development ◽

10.33448/rsd-v9i9.7742 ◽

2020 ◽

Vol 9 (9) ◽

pp. e487997742

Author(s):

Myrelle Leal Campos Sousa ◽

Isabela Alice Soares de Medeiros ◽

Liege Helena Freitas Fernandes ◽

Smyrna Luiza Ximenes de Souza ◽

Alidianne Fábia Cabral Cavalcanti ◽

...

Keyword(s):

Whey Protein ◽

Dental Enamel ◽

Soluble Solids ◽

Total Soluble Solids ◽

Significant Difference ◽

Vitro Analysis ◽

In Vitro Analysis

The objective of this study was to evaluate the pH and total soluble solids (TSS) content of whey protein beverages. Nine products of three different flavors (vanilla, strawberry and chocolate) of the following brands Muke®, Best Whey®, and Essencial Nutrition Whey®, had their pH evaluated by potentiometry, and their TSS evaluated by refractometry. The lowest pH recorded was for Muke® Chocolate (5.75), and the highest was for Essencial Nutrition Whey® Cacao (6.91). Regarding TSS, the lowest average was for Essencial Nutrition Whey® Vanilla (10.17), and the highest was for Essencial Nutrition Whey® Red Berry (16.66). There was no statistically significant difference in pH and TSS among the flavors and brands evaluated. It was concluded that the analyzed beverages had a higher pH than the pH that is critical to dental enamel and the TSS content was considered high.

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Chemical constituents and in-vitro rumen fermentation for dietary inclusion of Delonix regia seed meal for sustainable ruminant production

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i5.270 ◽

2020 ◽

Vol 46 (5) ◽

Author(s):

A. A. Lamidi ◽

J. I. Wilson ◽

O. O. Joseph

Keyword(s):

Chemical Constituents ◽

Gas Production ◽

Metabolizable Energy ◽

In Vitro Digestibility ◽

Delonix Regia ◽

Significant Difference ◽

Experimental Treatments ◽

Vitro Analysis ◽

In Vitro Analysis

This study was conducted to assess the chemical composition and in-vitro digestibility for dietary inclusion of Delonix regia seeds meal (DSM) for sustainable ruminant production. Four experimental treatments were formulated and DSM was included and varied as T1 (35/0%), T2 (20/15%), T3 (15/20%) and T4 (0/35%), respectively, each treatment were replicated and analyzed for the proximate composition and in-vitro analysis. There were significant differences (P<0.05) in the nutrients and fiber fractions (CP, EE, ash, OM, NFE, NDF, ADF and hemicellulose) across the experimental treatments as CP ranged between 10.50 – 13.60% and decreased with inclusion of DSM. The NFE, NDF and hemicelluloses were similar (P>0.05) in T1 and T4. In-vitro analysis indicated that the methane gas volume was significantly (P<0.05) influenced with the dietary inclusion of DSM across the experimental treatments as T2 had the highest volume of CH (0.61mL) while the least was recorded for T1 (0.47 mL). There was no significant difference (P>0.05) for 24hrs gas production, CH percentage, CH reduction percentage, in-vitro dry matter digestibility (IDMD), in-vitro organic matter digestibility (IOMD), metabolizable energy and fermentation efficiency across the treatments. Conclusively, the nutrients profile of the experimental treatments justifies the utilization of DSM for sustainable ruminant production. Similarity in post in-vitro parameters across treatments is an indication that inclusion of DSM will go a long way to resolve the challenges of feed and feeding for ruminant production.

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Dump the pump: manual aspiration thrombectomy (MAT) with a syringe is technically effective, expeditious, and cost-efficient

Journal of NeuroInterventional Surgery ◽

10.1136/neurintsurg-2017-013520 ◽

2017 ◽

Vol 10 (4) ◽

pp. 354-357 ◽

Cited By ~ 10

Author(s):

Bradley A Gross ◽

Ashutosh P Jadhav ◽

Tudor G Jovin ◽

Brian Thomas Jankowitz

Keyword(s):

Cost Effective ◽

Clinical Results ◽

Similar Efficacy ◽

Aspiration Thrombectomy ◽

Clinical Series ◽

Efficient Alternative ◽

Cost Efficient ◽

Vitro Analysis ◽

In Vitro Analysis

IntroductionSyringe aspiration for manual aspiration thrombectomy (MAT) is a cost- and time-efficient alternative to an aspiration pump with likely similar efficacy. It is counterintuitive to expect the pump to perform better than direct vacuum with a syringe, as the pump must deliver vacuum additionally through a canister and meters of tubing.ObjectiveTo present in vitro and clinical results of MAT with a syringe.MethodsAn in vitro analysis was performed comparing vacuum pressures generated by syringe aspiration and with pump aspiration. This was then complemented with prospective clinical data providing details of angiographic and clinical outcomes for syringe MAT.ResultsThe in vitro analysis demonstrated that equal to slightly greater vacuum pressures were generated by a 60 cc syringe as compared with the pump in both static and partial flow conditions. In our clinical series, 106/113 acute stroke thrombectomies over a 6-month period were performed with syringe MAT on the first pass. Syringe usage instead of pump tubing and a canister led to a total savings of $58 300. The rate of Thrombolysis in Cerebral Infarction 2b/3 recanalization was 93%. Adjunctive stentriever usage was performed in 23% of cases. Median puncture to reperfusion time was 25 min; mean change in National Institute of Health Stroke Scale score at 24 hours was an improvement of 5.1 (median 6). The in-hospital mortality rate was 10%. Seventy percent of patients were discharged to home (modified Rankin Scale (mRS) score 0–2) or a rehabilitation facility (mRS score 2–4).ConclusionMAT using a syringe is a safe, fast, and more cost-effective approach than using an aspiration pump.

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The native structure of the eukaryotic ribosome

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075890 ◽

1983 ◽

Vol 41 ◽

pp. 432-433

Author(s):

R.A. Milligan ◽

P.N.T. Unwin

Keyword(s):

Ribosomal Proteins ◽

Crystal Form ◽

Native Structure ◽

X Ray Diffraction ◽

Eukaryotic Ribosome ◽

Vitro Analysis ◽

In Vitro Analysis ◽

Resolution Structure ◽

Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

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