scholarly journals Immunofluorescence Evaluation of Myf5 and MyoD in Masseter Muscle of Unilateral Posterior Crossbite Patients

2020 ◽  
Vol 5 (4) ◽  
pp. 80
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Guglielmo Ramieri ◽  
Michele Tepedino ◽  
Michele Runci Anastasi ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Masseter Muscle ◽  
Muscle Hypertrophy ◽  
Contralateral Side ◽  
Surgical Patients ◽  
Posterior Crossbite ◽  
Fibre Formation ◽  
Masseter Muscles ◽  
Low Activity ◽  
Contralateral Muscle

A unilateral posterior crossbite is a malocclusion where the low activity of the affected masseter muscle is compensated by the contralateral muscle hypertrophy. It is still unknown if, in the same condition, myogenesis with new fibre formation takes place. Aim: the aim of the present study was to evaluate the expression of myogenesis markers, such as Myf5 and MyoD, in masseter muscles of unilateral posterior crossbite patients. Materials and methods: biopsies from fifteen surgical patients with unilateral posterior crossbites have been analysed by immunofluorescence reactions. The results show the expression of Myf5 and MyoD in the contralateral muscle but not in the ipsilateral one. Moreover, statistical analysis shows the higher number of satellite cells in the contralateral side if compared to the ipsilateral one. Conclusions: these results suggest that in contralateral muscle, hyperplastic events take place, as well as hypertrophy.

scholarly journals Extracellular Matrix Behaviour in Masseter Muscle of Patients Affected by Unilateral Posterior Crossbite: An Immunofluorescence Study

2021 ◽  
Vol 11 (10) ◽  
pp. 4649
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Maria Grazia Piancino ◽  
Maria Chiara Malandrino ◽  
Michele Runci Anastasi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Masseter Muscle ◽  
Muscle Hypertrophy ◽  
Contralateral Side ◽  
Collagen Iv ◽  
Hypertrophic Response ◽  
Posterior Crossbite ◽  
Increased Activity ◽  
Reduced Activity ◽  
Contralateral Muscle

Unilateral posterior crossbite is a malocclusion disease that involves morpho-functional characteristics of masseter muscle; a normal or increased activity of contralateral muscle and a reduced activity of the ipsilateral muscle during unilateral crossbite have been shown. Since the extracellular matrix plays a key role in in mechano-transduction of transmitting forces during muscle contraction, the aim of the present study was to analyse the behaviour of extracellular matrix in this type of malocclusion through immunofluorescence reactions against laminin, collagen IV, MMP-2 and MMP-9. Our results show an increased expression of Laminin, Collagen IV, and MMP-9 in the contralateral side if compared to the ipsilateral side. No differences have been found in MMP-2 expression between contralateral and ipsilateral muscles. Since the increased expression of Laminin, Collagen IV and MMP-9 is associated with muscle hypertrophy and MMP-2 is associated with muscle atrophy, our results support the existence of a hypertrophic response of contralateral muscle during unilateral posterior crossbite that probably aims to compensate the altered function of the ipsilateral one.

scholarly journals Histological, Histochemical, and Protein Changes after Induced Malocclusion by Occlusion Alteration of Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Carolina de Souza Guerra ◽  
Yamba Carla Lara Pereira ◽  
João Paulo Mardegan Issa ◽  
Kelly Galisteu Luiz ◽  
Elaine A. Del Bel Guimarães ◽  
...  
Keyword(s):  
Masseter Muscle ◽  
Wistar Rats ◽  
Morphological Analysis ◽  
Contralateral Side ◽  
Environmental Interventions ◽  
Stomatognathic System ◽  
Masseter Muscles ◽  
Experimental Group ◽  
Muscle Remodeling

Although disorders of the stomatognathic system are common, the mechanisms involved are unknown. Our objective was to study the changes in the masseter muscles after unilateral exodontia. Molar extraction was performed on Wistar rats (left side), and the animals were sacrificed after either 14 or 26 days. The masseter muscle was processed for histological analysis, conventional andin situzymography, and immunohistochemistry. The morphological analysis showed unique and specific characteristics for the experimental group. By conventional zymography no significant values of 72 kDa MMP-2 (P< 0.05) were found in both of the sides of masseter muscle after 14 and 26 days of unilateral extraction. Thein situzymography showed gelatinolytic activity on all deep masseter muscles, with significant increase on the contralateral side after 14 and 26 days (P< 0.05). The immunohistochemistry demonstrated greater expression of MMP-2 than MMP-9 and MMP-14 in all masseter muscles and there were few differences in the staining of 4 TIMPs. This knowledge about morphology and molecular masticatory muscle remodeling following environmental interventions can be used to develop clinically successful treatments.

scholarly journals Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study

2021 ◽  
Vol 11 (12) ◽  
pp. 5350
Author(s):  
Giovanna Vermiglio ◽  
Mariagrazia Piancino ◽  
Michele Runci Anastasi ◽  
Giacomo Picciolo ◽  
Antonio Centofanti ◽  
...  
Keyword(s):  
Masseter Muscle ◽  
Muscle Fibers ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Posterior Crossbite ◽  
Affected Side ◽  
The Cross ◽  
And Control ◽  
Contralateral Muscle

Unilateral posterior crossbite is a type of malocclusion that involves morpho-functional characteristics of masticatory muscle, such as the masseter: electrophysiological data have shown that the affected side works less than the contralateral muscle, which shows a normal or increased activity, probably in order to compensate for the affected side. The aim of present work was to measure the diameter and the cross-sectional area of ipsilateral and contralateral muscle fibers to verify if hypertrophy and/or hypotrophy take place in this malocclusion. We used immunofluorescence pictures to measure, using ImageJ software, the diameter and the cross-sectional area of fibers from control and crossbite groups; after that, the data were processed to perform statistical analyses. Results show that the fiber diameters of contralateral muscle are larger than the diameters of ipsilateral and control fibers, and that this difference is statistically significant. No statistically significant difference was found between the fiber diameters of the ipsilateral and control muscles. All these data suggest that, during unilateral posterior crossbite, morphological changes take place in the contralateral masseter muscle, which undergoes hypertrophy, probably to compensate for the low activity of the affected muscle.

scholarly journals Masseter muscle hypertrophy: case report

2006 ◽  
Vol 17 (4) ◽  
pp. 347-350 ◽  
Author(s):  
Eduardo Kazuo Sannomya ◽  
Marcelo Gonçalves ◽  
Marcelo Paraíso Cavalcanti
Keyword(s):  
Magnetic Resonance Imaging ◽  
Computed Tomography ◽  
Masseter Muscle ◽  
Muscle Hypertrophy ◽  
Mouth Opening ◽  
Conventional Radiography ◽  
Dental Infection ◽  
Resonance Imaging ◽  
Males And Females ◽  
Masseter Muscles

Masseter muscle hypertrophy is characterized by unilateral or bilateral enlargement of the masseter muscles affecting both males and females after puberty. Its etiology remains unknown. Limitations on mouth opening and also tension in the region of the hypertrophied muscle are symptoms reported. This paper reports a case of masseter muscle hypertrophy diagnosed using imaging modalities such as conventional radiography, computed tomography and magnetic resonance imaging scans. The familiarity with this condition is important to settle the differential diagnosis with other pathologies such as parotid gland tumors and dental infection.

scholarly journals Management of Unilateral Masseter Hypertrophy and Hypertrophic Scar—A Case Report

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Naresh Shetty ◽  
Rajanikanth K. Malaviya ◽  
M. K. Gupta
Keyword(s):  
Case Report ◽  
Hypertrophic Scar ◽  
Masseter Muscle ◽  
Muscle Hypertrophy ◽  
Treatment Options ◽  
Facial Asymmetry ◽  
Rare Condition ◽  
Keloid Scar ◽  
Masseter Muscles ◽  
Single Approach

Masseter muscle hypertrophy is a rare condition of idiopathic cause. It clinically presents as an enlargement of one or both masseter muscles. Most patients complain of facial asymmetry; however, symptoms such as trismus, protrusion, and bruxism may also occur. Several treatment options reported for masseter hypertrophy are present, which range from simple pharmacotherapy to more invasive surgical reduction. Keloid scar with unilateral masseter hypertrophy is a rarely seen in clinical practice. This paper reports a case of unilateral masseter hypertrophy with keloid scar in the angle of the mandible for which surgical treatment was rendered to the patient by using a single approach.

scholarly journals Morphofunctional compensation of masseter muscles in unilateral posterior crossbite patients

2016 ◽  
Vol 60 (2) ◽  
Author(s):  
G. Cutroneo ◽  
G. Vermiglio ◽  
A. Centofanti ◽  
G. Rizzo ◽  
M. Runci ◽  
...  
Keyword(s):  
Masticatory Muscles ◽  
Contralateral Side ◽  
Dental Arch ◽  
Ipsilateral Side ◽  
Hypertrophic Response ◽  
Posterior Crossbite ◽  
And Function ◽  
Relationship Of ◽  
Masseter Muscles

Unilateral posterior crossbite is a widespread, asymmetric malocclusion characterized by an inverse relationship of the upper and lower buccal dental cusps, in the molar and premolar regions, on one side only of the dental arch. Patients with unilateral posterior crossbite exhibit an altered chewing cycles and the crossbite side masseter results to be less active with respect to the contralateral one. Few studies about morphological features of masticatory muscle in malocclusion disorders exist and most of these have been performed on animal models. The aim of the present study was to evaluate morphological and protein expression characteristics of masseter muscles in patients affected by unilateral posterior crossbite, by histological and immunofluorescence techniques. We have used antibody against PAX-7, marker of satellite cells, and against α-, β-, γ-, δ-, ε- and ζ-sarcoglycans which are transmembrane glycoproteins involved in sarcolemma stabilization. By statistical analysis we have evaluated differences in amount of myonucley between contralateral and ipsilateral side. Results have shown: i) altered fibers morphology and atrophy of ipsilateral muscle if compared to the contralateral one; ii) higher number of myonuclei and PAX-7 positive cells in contralateral side than ipsilateral one; iii) higher pattern of fluorescence for all tested sarcoglycans in contralateral side than ipsilateral one. Results show that in unilateral posterior crossbite hypertrophic response of contralateral masseter and atrophic events in ipsilateral masseter take place; by that, in unilateral posterior crossbite malocclusion masticatory muscles modify their morphology depending on the function. That could be relevant in understanding and healing of malocclusion disorders; in fact, the altered balance about structure and function between ipsilateral and contralateral muscles could, long-term, lead and/ or worsen skeletal asymmetries.

scholarly journals Masseter muscle surface electromyography in college students with a high degree of anxiety and temporomandibular disorder

Revista CEFAC ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Eduarda de Lima Amarante ◽  
Jully Anne Soares de Lima ◽  
Rafael Nóbrega Bandeira ◽  
Ana Paula Arruda de Moura ◽  
Luciane Spinelli de Figueiredo Pessoa ◽  
...  
Keyword(s):  
College Students ◽  
Electrical Activity ◽  
Masseter Muscle ◽  
Speech Therapy ◽  
Temporomandibular Disorder ◽  
P Value ◽  
Voluntary Activity ◽  
Group 2 ◽  
High Degree ◽  
Masseter Muscles

ABSTRACT Objective: to compare the electrical activity of masseter muscles, bilaterally, according to the presence or absence of Temporomandibular Disorder (TMD) in college students with a high degree of anxiety. Methods: the study was conducted with a randomized sample of 31 Speech Therapy students aged between 17 and 32 years; 61.3% (n = 19) were females and 38.7% (n = 12) were males. They were divided into two groups, Group 1 (G1), comprising 11 students with TMD, and Group 2 (G2), composed of 20 students without TMD. The college students answered the State-Trait Anxiety Inventory (STAI) for anxiety investigation, and were evaluated by the protocol Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) for TMD diagnosis. The evaluation of muscular electrical activity took into account the records in the conditions of rest, Sustained Maximum Voluntary Activity (SMVA) and habitual chewing (HC). The data were analyzed using the version 22 IBM Statistical Package for Social Sciences (SPSS) software. The statistical analysis was performed using Student t test to compare means between groups, considering < 0,05 as the significant p-value. Results: college students, of both groups, presented high levels of anxiety traits. Significant statistical differences were observed on the percentage of electrical activity of right masseter muscle in chewing function, as well as muscle fibers recruitment during chewing, which were higher on the group without TMD. Conclusion: college students with TMD and a high degree of anxiety presented lower means of masseter muscle electromyografic activity during chewing, in most conditions assessed, as compared to volunteers without TMD, except for the left masseter muscle in rest and chewing.

scholarly journals Stiffness of the Masseter Muscle in Children—Establishing the Reference Values in the Pediatric Population Using Shear-Wave Elastography

2021 ◽  
Vol 18 (18) ◽  
pp. 9619
Author(s):  
Cyprian Olchowy ◽  
Anna Olchowy ◽  
Aleksander Pawluś ◽  
Mieszko Więckiewicz ◽  
Luca Maria Sconfienza
Keyword(s):  
Shear Wave ◽  
Masseter Muscle ◽  
Pediatric Population ◽  
Shear Wave Elastography ◽  
Total Sample ◽  
Healthy Children ◽  
Significant Difference ◽  
The Right ◽  
The Impact ◽  
Masseter Muscles

In children, the quality and muscle function are altered in many pathologic conditions, including temporomandibular disorders. Although several methods have been used to evaluate muscle tonus, none became a golden standard. Moreover, the masseter muscle characteristics in children have not been investigated to date. This study aimed to measure the stiffness of the masseter muscle using shear-wave elastography in healthy children. We enrolled 30 healthy children (mean age 10.87 ± 3.38 years). The stiffness of masseter muscles was measured with shear wave elastography. Stiffness for the total sample was 6.37 ± 0.77 kPa. A comparison of the measurements did not show significant differences between the right and the left masseter muscles (left—6.47 ± 0.78 kPa; right—6.24 ± 0.76 kPa; p = 0.3546). A significant difference was seen between boys and girls (boys—5.94 ± 0.50 kPa; girls—6.63 ± 0.80; p = 0.0006). Shear-wave elastography is a promising diagnostic tool. It may help to detect changes in the stiffness of the masseter muscle and draw attention to pathological processes within the jaw muscles. Directions for further research shall include determining stiffness values in pathological conditions and the impact of biological and functional factors on the stiffness of the masseter muscle.

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