scholarly journals The First Whole Genome Sequencing of Sanghuangporus sanghuang Provides Insights into Its Medicinal Application and Evolution

2021 ◽  
Vol 7 (10) ◽  
pp. 787
Author(s):  
Ji-Hang Jiang ◽  
Sheng-Hua Wu ◽  
Li-Wei Zhou
Keyword(s):  
Genome Sequencing ◽  
Genome Sequence ◽  
Gene Families ◽  
Orthologous Genes ◽  
Protein Coding ◽  
Nutritional Strategy ◽  
Ancient People ◽  
Medicinal Properties ◽  
Comparative Genomics Analysis ◽  
First Time

Sanghuangporus is a medicinal macrofungal genus typified by S. sanghuang, the very species utilized in traditional Chinese medicines by Chinese ancient people. To facilitate the medicinal application of S. sanghuang, we, for the first time, perform its genome sequencing and analyses from a monokaryon strain. A 33.34 Mb genome sequence was assembled to 26 contigs, which lead to the prediction of 8278 protein-coding genes. From these genes, the potential biosynthesis pathway of sesquiterpenoids was, for the first time, identified from Sanghuangporus, besides that of triterpenoids. While polysaccharides are the main medicinal metabolites in S. sanghuang, flavonoids are especially abundant medicinal metabolites comparing with other medicinal macrofungal groups. From the genomic perspective, S. sanghuang has a tetrapolar heterothallic mating system, and has its special nutritional strategy and advantageous medicinal properties compared with S. baumii and S. vaninii. A phylogenomics analysis indicates that Sanghuangporus emerged 15.39 million years ago and S. sanghuang has a closer phylogenetic relationship with S. baumii than S. vaninii. However, S. sanghuang shares a higher region of synteny and more orthologous genes, including carbohydrate-active enzymes with S. vaninii than S. baumii. A comparative genomics analysis with S. baumii and S. vaninii indicates that species diversification within Sanghuangporus may be driven by the translocation and translocation plus inversion of genome sequences, while the expansion and contraction of gene families may contribute to the host specificity of Sanghuangporus species. In general, the genome sequence of S. sanghuang provides insights into its medicinal application and evolution.

scholarly journals Genome Sequencing of a Novel Coronavirus SARS-CoV-2 Isolate from Iraq

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Nihad A. M. Al-Rashedi ◽  
Danilo Licastro ◽  
Sreejith Rajasekharan ◽  
Simeone Dal Monego ◽  
Alessandro Marcello ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Illumina Miseq ◽  
Spike Protein ◽  
Protein Coding ◽  
Novel Coronavirus ◽  
First Time

ABSTRACT The coding-complete genome sequence of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain isolated from an Iraqi patient was sequenced for the first-time using Illumina MiSeq technology. There was a D614G mutation in the spike protein-coding sequence. This report is valuable for better understanding the spread of the virus in Iraq.

scholarly journals Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Huihui Du ◽  
Rendong Fang ◽  
Tingting Pan ◽  
Tian Li ◽  
Nengzhang Li ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Genome Sequence ◽  
Pasteurella Multocida ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Insertion Sequences ◽  
Capsular Type ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Comparative Genomics Analysis

The Pasteurella multocida capsular type A isolates can cause pneumonia and bovine respiratory disease (BRD). In this study, comparative genomics analysis was carried out to identify the virulence genes in two different virulent P. multocida capsular type A isolates (high virulent PmCQ2 and low virulent PmCQ6). The draft genome sequence of PmCQ2 is 2.32 Mbp and contains 2,002 protein-coding genes, 9 insertion sequence (IS) elements, and 1 prophage region. The draft genome sequence of PmCQ6 is 2.29 Mbp and contains 1,970 protein-coding genes, 2 IS elements, and 3 prophage regions. The genome alignment analysis revealed that the genome similarity between PmCQ2 and PmCQ6 is 99% with high colinearity. To identify the candidate genes responsible for virulence, the PmCQ2 and PmCQ6 were compared together with that of the published genomes of high virulent Pm36950 and PmHN06 and avirulent Pm3480 and Pm70 (capsular type F). Five genes and two insertion sequences are identified in high virulent strains but not in low virulent or avirulent strains. These results indicated that these genes or insertion sequences might be responsible for the virulence of P. multocida, providing prospective candidates for further studies on the pathogenesis and the host-pathogen interactions of P. multocida.

scholarly journals Gene recruitments and dismissals in argonaut octopus genome provide insights to pelagic lifestyle adaptation and shell-like eggcase reacquisition

2021 ◽  
Author(s):  
Masa-aki Yoshida ◽  
Kazuki Hirota ◽  
Junichi Imoto ◽  
Miki Okuno ◽  
Hiroyuki Tanaka ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Average Length ◽  
Draft Genome ◽  
Gene Clusters ◽  
Draft Genome Sequence ◽  
Shell Formation ◽  
Protein Coding ◽  
Homologous Genes ◽  
Phenotypic Structure ◽  
Comparative Genomics Analysis

The paper nautilus, Argonauta argo, also known as the greater argonaut, is a species of octopods distinctly characterized by its pelagic lifestyle and by the presence of a spiral-shaped shell-like eggcase in females. The eggcase functions by protecting the eggs laid inside it, and by building and keeping air intakes for buoyancy. To reveal the genomic background of the species′ adaptation to pelagic lifestyle and the acquisition of its shell-like eggcase, we sequenced the draft genome sequence of the species. The genome size was 1.1 Gb, which is the smallest among the cephalopods known to date, with the top 215 scaffolds (average length 5,064,479 bp) covering 81% (1.09 Gb) of the total assembly. A total of 26,433 protein-coding genes were predicted from 16,802 assembled scaffolds. From these, we identified nearly intact HOX, Parahox, Wnt clusters and some gene clusters probably related to the pelagic lifestyle, such as reflectin, tyrosinase, and opsin. For example, opsin might have undergone an extensive duplication in order to adapt to the pelagic lifestyle, as opposed to other octopuses, which are mostly the benthic. Our gene models also discovered several genes homologous to those related to calcified shell formation in Conchiferan Mollusks, such as Pif-like, SOD, and TRX. Interestingly, comparative genomics analysis revealed that the homologous genes for such genes were also found in the genome of the octopus, which does not have a shell, as well as the basal cephalopods Nautilus. Therefore, the draft genome sequence of A. argo we presented here had not only helped us to gain further insights into the genetic background of the dynamic recruitment and dismissal of genes for the formation of an important, converging extended phenotypic structure such as the shell and the shell-like eggcase, but also the evolution of lifestyles in Cephalopods and the octopods, from benthic to pelagic.

scholarly journals The Genome Sequence of Mycoplasma hyopneumoniae Strain 232, the Agent of Swine Mycoplasmosis

2004 ◽  
Vol 186 (21) ◽  
pp. 7123-7133 ◽  
Author(s):  
F. Chris Minion ◽  
Elliot J. Lefkowitz ◽  
Melissa L. Madsen ◽  
Barbara J. Cleary ◽  
Steven M. Swartzell ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Antigenic Variation ◽  
Gene Families ◽  
Mycoplasma Hyopneumoniae ◽  
23S Rrna ◽  
Hypothetical Proteins ◽  
Protein Coding ◽  
Coding Sequences ◽  
Phase Switching ◽  
Transporter Family

ABSTRACT We present the complete genome sequence of Mycoplasma hyopneumoniae, an important member of the porcine respiratory disease complex. The genome is composed of 892,758 bp and has an average G+C content of 28.6 mol%. There are 692 predicted protein coding sequences, the average protein size is 388 amino acids, and the mean coding density is 91%. Functions have been assigned to 304 (44%) of the predicted protein coding sequences, while 261 (38%) of the proteins are conserved hypothetical proteins and 127 (18%) are unique hypothetical proteins. There is a single 16S-23S rRNA operon, and there are 30 tRNA coding sequences. The cilium adhesin gene has six paralogs in the genome, only one of which contains the cilium binding site. The companion gene, P102, also has six paralogs. Gene families constitute 26.3% of the total coding sequences, and the largest family is the 34-member ABC transporter family. Protein secretion occurs through a truncated pathway consisting of SecA, SecY, SecD, PrsA, DnaK, Tig, and LepA. Some highly conserved eubacterial proteins, such as GroEL and GroES, are notably absent. The DnaK-DnaJ-GrpR complex is intact, providing the only control over protein folding. There are several proteases that might serve as virulence factors, and there are 53 coding sequences with prokaryotic lipoprotein lipid attachment sites. Unlike other mycoplasmas, M. hyopneumoniae contains few genes with tandem repeat sequences that could be involved in phase switching or antigenic variation. Thus, it is not clear how M. hyopneumoniae evades the immune response and establishes a chronic infection.

scholarly journals Draft Genome Sequence of Salirhabdus euzebyi Strain Q1438

2020 ◽  
Vol 9 (18) ◽  
Author(s):  
Rita Zgheib ◽  
Hussein Anani ◽  
Didier Raoult ◽  
Pierre-Edouard Fournier
Keyword(s):  
Genome Sequencing ◽  
Genome Sequence ◽  
Bacterial Isolate ◽  
Draft Genome ◽  
Sea Salt ◽  
Whole Genome ◽  
Protein Coding ◽  
Content Type ◽  
Protein Coding Genes ◽  
Rna Genes

In 2007, Salirhabdus euzebyi was first described as a bacterial isolate from a sea salt evaporation pond. As no genome sequence was previously available for this species, we performed whole-genome sequencing. The chromosome of strain Q1438 was 3,784,443 bp long with 36% G+C content, 3,830 protein-coding genes, and 74 RNA genes.

scholarly journals Complete Genome Sequence of a New Megavirus Family Member Isolated from an Inland Water Lake for the First Time in India

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Anirvan Chatterjee ◽  
Farhan Ali ◽  
Disha Bange ◽  
Kiran Kondabagil
Keyword(s):  
Family Member ◽  
Genome Size ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Dna Virus ◽  
Double Stranded Dna ◽  
Water Lake ◽  
First Time

We report here the isolation and complete genome sequencing of a large double-stranded DNA virus, Powai Lake megavirus, for the first time from India. The isolation of a large DNA virus with genome size >1 Mb from India further attests to the prevalence of Giant viruses in different environmental niches.

scholarly journals Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Livingstone 1236H, a Desiccation-Resistant Strain That Poses a Salmonellosis Hazard in Low-Moisture Foods

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Ahmed G. Abdelhamid ◽  
Yumin Xu ◽  
Ahmed E. Yousef
Keyword(s):  
Genome Sequencing ◽  
Genome Sequence ◽  
Resistant Strain ◽  
Salmonella Enterica ◽  
Peanut Butter ◽  
Draft Genome ◽  
Protein Coding ◽  
Coding Sequences ◽  
Content Type ◽  
Low Moisture Foods

ABSTRACT Salmonella enterica serovar Livingstone 1236H was isolated originally from peanut butter and represents a health risk in low-moisture foods. The current work presents the strain’s genome sequencing results, which show a 4,824,729-bp genome sequence and 4,435 protein coding sequences, including some that are involved in adaptation to low-moisture environments.

scholarly journals Coexistence of Two Different Photosynthetic Operons in Citromicrobium bathyomarinum JL354 As Revealed by Whole-Genome Sequencing

2009 ◽  
Vol 192 (4) ◽  
pp. 1169-1170 ◽  
Author(s):  
Nianzhi Jiao ◽  
Rui Zhang ◽  
Qiang Zheng
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Marine Environment ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Whole Genome ◽  
Single Strain ◽  
Anoxygenic Phototrophic Bacterium ◽  
First Time

ABSTRACT Citromicrobium bathyomarinum is a bacteriochlorophyll a-containing aerobic anoxygenic phototrophic bacterium. Here, we report the draft genome sequence of C. bathyomarinum JL354, which was the first C. bathyomarinum strain isolated from a euphotic marine environment. The JL354 genome sequence reveals, for the first time, the coexistence of two different photosynthetic operons in a single strain.

scholarly journals Advanced whole genome sequencing and analysis of fetal genomes from amniotic fluid

2017 ◽  
Author(s):  
Qing Mao ◽  
Robert Chin ◽  
Weiwei Xie ◽  
Yuqing Deng ◽  
Huixin Xu ◽  
...  
Keyword(s):  
Autism Spectrum Disorder ◽  
Amniotic Fluid ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Chromosomal Abnormalities ◽  
Autism Spectrum ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
First Time

Amniocentesis is typically performed to identify large chromosomal abnormalities within the fetus. Here we demonstrate that it is feasible to generate an accurate whole genome sequence (WGS) of a fetus from an amniotic sample. DNA from cells and the amniotic fluid were isolated and sequenced from 31 amniocenteses. Concordance of variant calls between the two DNA sources and with parental libraries was high. Two fetal genomes were found to harbor potentially detrimental variants in CHD8 and LRP1, variations in these genes have been associated with Autism Spectrum Disorder (ASD) and Keratosis pilaris atrophicans, respectively. We also discovered drug sensitivities and carrier information of fetuses for a variety of diseases. In this study, we demonstrate for the first time the sequencing of the whole genome of fetuses from amniotic fluid and show that much more information than large chromosomal abnormalities can be gained from an amniocentesis.

scholarly journals First Genome Sequence of Shallot Latent Carlavirus from Allium macrostemon Bunge

2017 ◽  
Vol 5 (33) ◽  
Author(s):  
Kazusato Ohshima ◽  
Kouta Okamura ◽  
Ryosuke Yasaka ◽  
Shinji Fukuda ◽  
Kanji Ishimaru ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Genome Sequence ◽  
Genomic Sequence ◽  
Allium Macrostemon ◽  
First Time

ABSTRACT A wild Japanese garlic plant (Allium macrostemon Bunge, wild onion) with leaves showing chlorotic stripes was collected in Saitama Prefecture, Japan. Genome sequencing showed that it was infected with shallot latent carlavirus. The genomic sequence of this virus is reported for the first time from wild onion.

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