scholarly journals Comparison of Bacterial Community in Paddy Soil after Short-Term Application of Pig Manure and the Corresponding Organic Fertilizer

Land ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 9
Author(s):  
Min Wang ◽  
Chenyan Sha ◽  
Jianqiang Wu ◽  
Peng Li ◽  
Juan Tan ◽  
...  
Keyword(s):  
Paddy Soil ◽  
Pathogenic Bacteria ◽  
High Throughput Sequencing ◽  
Organic Fertilizer ◽  
Microbial Community Composition ◽  
Soil Health ◽  
Paddy Fields ◽  
Pig Manure ◽  
Ripening Stage ◽  
Short Term

Agricultural fertilization significantly affects nutrients cycling in paddy soils. However, there are few systematic studies on the response of the application of livestock manure and corresponding organic fertilizer resulting in the microorganism and its nutrients entering farmland. Short-term application of pig manure and its corresponding organic fertilizer on the microorganisms of paddy fields were investigated. High-throughput sequencing technology was conducted to comprehensively study the microbial community composition and structure. Our results showed that organic fertilizer effectively enhanced nutrient substances such as nitrogen, phosphorus, and potassium at ripening stage and reduced the pH of soil, benefitting the soil fertility and the growth of rice. Though application of pig manure maintained higher microbial diversity, it increased the relative abundance of several pathogenic bacteria, which could threaten the soil health of the paddy fields. A large number of pathogenic bacteria can be reduced through composting pig manure to organic fertilizer. Nitrifying and denitrifying bacteria such as Anaerolineaceae, Pseudarthrobacter, Bacillus, and Nitrospira in the paddy soil were significantly promoted. The heavy metals such as Pb, Cr, and Cd, and nutrient substances such as phosphorus, as well as temperature, have important influences on the microbial compositions in ripening stage. The correlation analysis revealed more correlation efficiencies were observed with manure application, especially with the application of organic fertilizer. This study will provide a theoretical basis for improving land productivity and sustainable development in paddy fields.

Evaluation of Composted Pig Manure and Organic Fertilizer for Organic Onion Production in Paddy Soil

2012 ◽  
Vol 30 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Jong-Tae Lee ◽  
Hee-Dae Kim ◽  
Sang-Dae Lee ◽  
Chi-Woong Ro
Keyword(s):  
Paddy Soil ◽  
Organic Fertilizer ◽  
Pig Manure

scholarly journals Effect of Organic Fertilizer on Soil Bacteria in Maize Fields

Land ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 328
Author(s):  
Jianqiang Wu ◽  
Chenyan Sha ◽  
Min Wang ◽  
Chunmei Ye ◽  
Peng Li ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Soil Bacteria ◽  
Pathogenic Bacteria ◽  
Organic Fertilizer ◽  
Growth Cycle ◽  
Fertilizer Application ◽  
Mature Stage ◽  
Pig Manure ◽  
Available Phosphorus ◽  
Driving Factor

Soil microorganisms play an important role in agricultural ecosystems, but their response to organic fertilizer application has not been thoroughly elucidated. Thus, high-throughput sequencing was used to investigate the responses of soil bacterial to organic fertilizer amendment (composted from pig manure) in the field during the entire growth cycle of maize plants. Four treatments were studied: treatment with 2 kg·m–2 organic fertilizer application (OF_2), treatment with 4 kg·m–2 organic fertilizer application (OF_4), treatment with 6 kg·m–2 organic fertilizer application (OF_6), and a controlled treatment (CK) without fertilization. The results revealed that the bacterial richness in OF_2 was significantly lower than that of CK (p < 0.05). Soil eutrophication bacteria Bacteroidetes increased effectively in all fertilized soils, relative abundance in OF_2, OF_4, and OF_6 for the entire maize growth cycle was 68.00%, 71.40%, and 77.93% higher than that in CK, respectively. In addition, soil nitrobacteria (Nitrospirae, Nitrospira), were markedly decreased (p < 0.05) with fertilization amount. The relative abundance of the nitrogen-fixing genus Adhaeribacter in OF_6 was 209.28%, 72.8% and 35.66% higher than that in CK, OF_2 and OF_4 at mature stage. The pathogenic genus Flavolibacterium was significantly increased (p < 0.05) in fertilized soil at the seeding stage. The driving factor governing the variations of bacterial community in CK, OF_2, OF_4 and OF_6 were pH value, available phosphorus, available phosphorus, and chromium, respectively. The findings highlight that part of the soil functional or pathogenic bacteria population was susceptible to organic fertilizer application; and the driving factor of bacterial composition change was associated with the rate of fertilization. More targeted experiments are needed to enhance the understanding of functional bacteria and the synergistic effect of soil physicochemical property physical on soil bacteria.

scholarly journals PELATIHAN TEKNIS PEMBUATAN KOMPOS JERAMI PADI DI DESA BANJARREJO KECAMATAN BATANGHARI KABUPATEN LAMPUNG TIMUR

2018 ◽  
Vol 3 (1) ◽  
pp. 6-14
Author(s):  
Dulbari Dulbari ◽  
Yuriansyah Yuriansyah ◽  
Zainal Mutaqin ◽  
Lisa Erfa ◽  
I Gde Darmaputra
Keyword(s):  
Soil Fertility ◽  
Rice Straw ◽  
Community Service ◽  
Main Product ◽  
Organic Fertilizer ◽  
Soil Health ◽  
External Input ◽  
Technical Training ◽  
Paddy Fields ◽  
Lack Of Information

The level of fertility of paddy fields in Banjarrejo was decreasing, this is indicated by low rice productivity, fertilization response was leveling off, topsoil was shallow, and rice easy lodged. Fertility degradation of paddy fields occurs continuously. Factors that encourage the ongoing process of soil fertility degradation is a concept application of High External Input Agriculture (HEIA) on the cultivation of crops, especially rice. Rice straw can be used as an alternative to increase soil fertility and maintain the health of paddy field. Straw is the main product of rice cultivation in the form of organic fertilizer because its potential reaches 1.5 x grain yield. However, the potential that is available cheaply and easily and Lack of information and knowledge about straw causes many farmers not to utilize straw to the fullest. Straw is still regarded as an obstacle in tillage, where the nest mice, disease sources, and sources of dirt that must be cleaned. Farmers have not used it to be composted because many still do not have the skills to make it. State Polytechnic of Lampung has the role and responsibility to transfer technology that is beneficial to society especially in the case of making straw compost through the program of Community Service. Technical Training Composting of Rice Straw was conducted in Banjarrejo Village, Batanghari District, East Lampung Regency in April-September 2013. The use of straw compost in paddy fields can increase rice production while maintaining sustainable soil health.

scholarly journals First reported quantitative microbiota in different livestock manures used as organic fertilizers in the Northeast of Thailand

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lampet Wongsaroj ◽  
Ratmanee Chanabun ◽  
Naruemon Tunsakul ◽  
Pinidphon Prombutara ◽  
Somsak Panha ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Organic Fertilizer ◽  
The Other ◽  
Organic Fertilizers ◽  
Rrna Gene ◽  
Bacterial Composition ◽  
Physiochemical Properties ◽  
Nutrient Profiles ◽  
Soil Recovery ◽  
Local Farmers

AbstractNortheastern Thailand relies on agriculture as a major economic activity, and has used high levels of agrochemicals due to low facility, and salty sandy soil. To support soil recovery and sustainable agriculture, local farmers have used organic fertilizers from farmed animal feces. However, knowledge about these animal fecal manures remains minimal restricting their optimal use. Specifically, while bacteria are important for soil and plant growth, an abundance and a diversity of bacterial composition in these animal fecal manures have not been reported to allow selection and adjustment for a more effective organic fertilizer. This study thereby utilized metagenomics combined with 16S rRNA gene quantitative PCR (qPCR) and sequencing to analyze quantitative microbiota profiles in association with nutrients (N, P, K), organic matters, and the other physiochemical properties, of the commonly used earthworm manure and other manures from livestock animals (including breed and feeding diet variations) in the region. Unlike the other manures, the earthworm manure demonstrated more favorable nutrient profiles and physiochemical properties for forming fertile soil. Despite low total microbial biomass, the microbiota were enriched with maximal OTUs and Chao richness, and no plant pathogenic bacteria were found based on the VFDB database. The microbial metabolic potentials supported functions to promote crop growth, such as C, N and P cyclings, xenobiotic degradation, and synthesis of bioactive compounds. Pearson’s correlation analyses indicated that the quantitative microbiota of the earthworm manure were clustered in the same direction as N, and conductivity, salinity, and water content were essential to control the microbiota of animal manures.

scholarly journals Seasonal Variation Characteristics of Bacteria and Fungi in PM2.5 in Typical Basin Cities of Xi’an and Linfen, China

Atmosphere ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 809
Author(s):  
Sen Wang ◽  
Wanyu Liu ◽  
Jun Li ◽  
Haotian Sun ◽  
Yali Qian ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Relative Abundance ◽  
Pathogenic Bacteria ◽  
High Throughput Sequencing ◽  
Fine Particulate Matter ◽  
Ecological Functions ◽  
Factors Affecting ◽  
Bacterial Phyla ◽  
Variation Characteristics ◽  
Bacteria And Fungi

Microorganisms existing in airborne fine particulate matter (PM2.5) have key implications in biogeochemical cycling and human health. In this study, PM2.5 samples, collected in the typical basin cities of Xi’an and Linfen, China, were analyzed through high-throughput sequencing to understand microbial seasonal variation characteristics and ecological functions. For bacteria, the highest richness and diversity were identified in autumn. The bacterial phyla were dominated by Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes. Metabolism was the most abundant pathway, with the highest relative abundance found in autumn. Pathogenic bacteria (Pseudomonas, Acinetobacter, Serratia, and Delftia) were positively correlated with most disease-related pathways. Besides, C cycling dominated in spring and summer, while N cycling dominated in autumn and winter. The relative abundance of S cycling was highest during winter in Linfen. For fungi, the highest richness was found in summer. Basidiomycota and Ascomycota mainly constituted the fungal phyla. Moreover, temperature (T) and sulfur dioxide (SO2) in Xi’an, and T, SO2, and nitrogen dioxide (NO2) in Linfen were the key factors affecting microbial community structures, which were associated with different pollution characteristics in Xi’an and Linfen. Overall, these results provide an important reference for the research into airborne microbial seasonal variations, along with their ecological functions and health impacts.

scholarly journals Effects of Short-Term Rice Straw Return on the Soil Microbial Community

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 561
Author(s):  
Enze Wang ◽  
Xiaolong Lin ◽  
Lei Tian ◽  
Xinguang Wang ◽  
Li Ji ◽  
...  
Keyword(s):  
Microbial Community ◽  
Rice Straw ◽  
Paddy Soil ◽  
Soil Microbial Community ◽  
Short Term ◽  
Soil Microbial ◽  
Microbial Network ◽  
Straw Return ◽  
Bacteria And Fungi ◽  
Main Factor

Rice straw is a byproduct of agricultural production and an important agricultural resource. However, rice straw has not yet been effectively used, and incorrect treatment methods (such as burning in the field) can cause serious damage to the environment. Studies have shown that straw returning is beneficial to soil, but there have been few studies focused on the effect of the amount of short-term straw returned on the soil microbial community. This study evaluates 0%, 50%, 75%, and 100% rice straw returned to the field on whether returning different amounts of straw in the short term would affect the diversity and composition of the soil microbial community and the correlation between bacteria and fungi. The results show that the amount of straw returned to the field is the main factor that triggers the changes in the abundance and composition of the microbial community in the paddy soil. A small amount of added straw (≤ 50% straw added) mainly affects the composition of the bacterial community, while a larger amount of added straw (> 50% straw added) mainly affects the composition of the fungal community. Returning a large amount of straw increases the microbial abundance related to carbon and iron cycles in the paddy soil, thus promoting the carbon and iron cycle processes to a certain extent. In addition, network analysis shows that returning a large amount of straw also increases the complexity of the microbial network, which may encourage more microbes to be niche-sharing and comprehensively improve the ecological environment of paddy soil. This study may provide some useful guidance for rice straw returning in northeast China.

scholarly journals Interactions between Rainbow Trout Eyed Eggs and Flavobacterium spp. Using a Bath Challenge Model: Preliminary Evaluation of Bacteriophages as Pathogen Control Agents

2021 ◽  
Vol 9 (5) ◽  
pp. 971
Author(s):  
Valentina L. Donati ◽  
Inger Dalsgaard ◽  
Anniina Runtuvuori-Salmela ◽  
Heidi Kunttu ◽  
Johanna Jørgensen ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Pathogenic Bacteria ◽  
Infection Model ◽  
Preliminary Evaluation ◽  
Fish Eggs ◽  
Short Term ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Pathogen Control ◽  
Number Of Bacteria ◽  
Bath Challenge

The microbial community surrounding fish eyed eggs can harbor pathogenic bacteria. In this study we focused on rainbow trout (Oncorhynchus mykiss) eyed eggs and the potential of bacteriophages against the pathogenic bacteria Flavobacterium psychrophilum and F. columnare. An infection bath method was first established, and the effects of singular phages on fish eggs was assessed (survival of eyed eggs, interaction of phages with eyed eggs). Subsequently, bacteria-challenged eyed eggs were exposed to phages to evaluate their effects in controlling the bacterial population. Culture-based methods were used to enumerate the number of bacteria and/or phages associated with eyed eggs and in the surrounding environment. The results of the study showed that, with our infection model, it was possible to re-isolate F. psychrophilum associated with eyed eggs after the infection procedure, without affecting the survival of the eggs in the short term. However, this was not possible for F. columnare, as this bacterium grows at higher temperatures than the ones recommended for incubation of rainbow trout eyed eggs. Bacteriophages do not appear to negatively affect the survival of rainbow trout eyed eggs and they do not seem to strongly adhere to the surface of eyed eggs either. Finally, the results demonstrated a strong potential for short term (24 h) phage control of F. psychrophilum. However, further studies are needed to explore if phage control can be maintained for a longer period and to further elucidate the mechanisms of interactions between Flavobacteria and their phages in association with fish eggs.

scholarly journals Disentangling Responses of the Subsurface Microbiome to Wetland Status and Implications for Indicating Ecosystem Functions

2021 ◽  
Vol 9 (2) ◽  
pp. 211
Author(s):  
Jie Gao ◽  
Miao Liu ◽  
Sixue Shi ◽  
Ying Liu ◽  
Yu Duan ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput Sequencing ◽  
Carbon Fixation ◽  
Microbial Community Composition ◽  
Ecosystem Functions ◽  
Microbial Composition ◽  
Wetland Ecosystems ◽  
Soil Microbial ◽  
Geochemical Properties ◽  
Microbial Groups

In this study, we analyzed microbial community composition and the functional capacities of degraded sites and restored/natural sites in two typical wetlands of Northeast China—the Phragmites marsh and the Carex marsh, respectively. The degradation of these wetlands, caused by grazing or land drainage for irrigation, alters microbial community components and functional structures, in addition to changing the aboveground vegetation and soil geochemical properties. Bacterial and fungal diversity at the degraded sites were significantly lower than those at restored/natural sites, indicating that soil microbial groups were sensitive to disturbances in wetland ecosystems. Further, a combined analysis using high-throughput sequencing and GeoChip arrays showed that the abundance of carbon fixation and degradation, and ~95% genes involved in nitrogen cycling were increased in abundance at grazed Phragmites sites, likely due to the stimulating impact of urine and dung deposition. In contrast, the abundance of genes involved in methane cycling was significantly increased in restored wetlands. Particularly, we found that microbial composition and activity gradually shifts according to the hierarchical marsh sites. Altogether, this study demonstrated that microbial communities as a whole could respond to wetland changes and revealed the functional potential of microbes in regulating biogeochemical cycles.

The Mechanism of Silkworm Excrement Organic Fertilizer to Reduce the Cd Availability in Paddy Soil

2021 ◽  
pp. 1-14
Author(s):  
Junxian Chen ◽  
Pengyang Bian ◽  
He Huang ◽  
Kai Zhong ◽  
Dan Huang ◽  
...  
Keyword(s):  
Paddy Soil ◽  
Organic Fertilizer ◽  
Cd Availability
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