scholarly journals Evaluation of Blood Coagulation Parameters and ADMA, NO, IL-6, and IL-18 Serum Levels in Patients with Neovascular AMD before, during, and after the Initial Loading Phase of Intravitreal Aflibercept

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 441
Author(s):  
Michał Wiciński ◽  
Małgorzata Seredyka-Burduk ◽  
Sławomir Liberski ◽  
Daria Marczak ◽  
Magdalena Pol ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Blood Coagulation ◽  
Serum Levels ◽  
Thrombin Time ◽  
Neovascular Amd ◽  
Coagulation Parameters ◽  
The Third ◽  
Initial Loading ◽  
Intravitreal Aflibercept ◽  
Coagulation Pathway

We evaluated the effect of intravitreal injections of aflibercept (IVA) on blood coagulation parameters including prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin time (TT), as well as asymmetric dimethylarginine (ADMA), nitric oxide (NO), interleukin 6 (IL-6), and interleukin 18 (IL-18) serum levels in patients with neovascular AMD (nAMD). Twenty-two eyes of 22 patients with nAMD were included. Parameters were evaluated before and 2–3 days after the first IVA injection, and then immediately before and 2–3 days after the third IVA injection. We revealed prolongation of the TT after the initial loading phase of IVA (p = 0.041) and a significant increase in IL-18 serum concentration immediately before the third IVA administration compared to baseline (p = 0.037). There were no statistically significant differences of other parameters and PT, APTT, ADMA, NO, and IL-6 values remained within the normal range at each of the time points of the study. Our results suggest that repeated IVA administration may affect the common blood coagulation pathway, which manifests as a prolongation of the TT value. Furthermore, we showed a significant increase in serum concentration of the pro-inflammatory cytokineIL-18during the initial loading phase of IVA.

scholarly journals Study of immediate changes in blood coagulation in an experimental model of ischemia/reperfusion injury of the kidney

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
N M B Gamil ◽  
A M Abdelrahman ◽  
D A A Darwish ◽  
E Ahmed
Keyword(s):  
Reperfusion Injury ◽  
Blood Coagulation ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Serum Levels ◽  
Renal Ischemia ◽  
Thrombin Time ◽  
Coagulation Parameters ◽  
Ischemic Reperfusion ◽  
Group I

Abstract Introduction Ischemia- reperfusion injury (IRI) in the kidney triggers multifaceted network of plasma cascades including the complement, coagulation, kinins, and fibrinolytic system, which play major role in the reperfusion-triggered inflammatory process. Aim of the work The aim of the present study was to investigate changes in blood coagulation parameters immediately after ischemia and after ischemia/reperfusion of the kidney in adult rats of both sexes. Materials and Methods The study was carried out on 56 adult albino rats of both sexes weighing 160-220 gm. Rats were randomly allocated into 5 experimental groups as follows: Control rat group (C; n = 10). Sham1- operated rat group (SH1; n = 10) subjected to all the procedures of the I rat group except for renal ischemia and were sacrificed after 45 minutes. Ischemic rat group (I; n = 10) subjected to renal ischemia for 45 min, then were sacrificed. Sham 2 operated rat group 2 (SH2; n = 10) subjected to all the procedures of IR – rat group except for renal ischemia and reperfusion and were sacrificed after 1½ hour. Ischemic/reperfusion rat group (IR; n = 10) subjected to renal ischemia for 45 min, then reperfusion for 45 minutes, then were immediately sacrificed. All rats were subjected to estimation of body weight (BW), absolute and relative kidney weights (AKW and RKW), hemoglobin (Hb) and hematocrit (Ht.) values, clotting time, prothrombin time (PT), activated partial thromboplastin time (PTT), thrombin time (TT) and serum levels of urea (Ur.) and creatinine (Cr.). Results I rat group showed insignificant changes in AKW and RKW, CT, APTT, PT and TT compared to SH1 and C rat groups. However they showed significant elevation in Hb content, Ht. value and serum levels of Ur. and Cr. compared to C rat group. IR rat group showed significant increase in AKW and RKW compared to SH2, I and C rat groups. Also they showed significant increase in Hb. content, Ht. value and serum levels of Ur. and Cr. compared to C rat group. However they showed no significant change in their CT, APTT, PT and TT compared to SH2, I and C rat groups Conclusion Coagulation parameters are not changed immediately after kidney ischemic/reperfusion and may not contribute to the immediate pathophysiological changes of ischemic reperfusion injury.

scholarly journals Implications of Blood Coagulation and Fibrinolytic Disorders in Severe Endometritis-Pyometra Complex in Bitches

2012 ◽  
Vol 56 (3) ◽  
pp. 293-297 ◽  
Author(s):  
Justyna Radwińska ◽  
Anna Domosławska ◽  
Andrzej Pomianowski ◽  
Katarzyna Żarczyńska ◽  
Andrzej Jurczak
Keyword(s):  
High Risk ◽  
Blood Coagulation ◽  
Disseminated Intravascular Coagulation ◽  
Partial Thromboplastin Time ◽  
Blood Platelet ◽  
Blood Parameters ◽  
Activated Partial Thromboplastin Time ◽  
Thrombin Time ◽  
Coagulation Parameters ◽  
D Dimer

Abstract Twenty bitches with acute endometritis-pyometra complex (EPC) and 20 clinically healthy bitches were examined. The following coagulation parameters were determined in haemostatic evaluations: prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), fibrinogen concentrations (FBG), D-dimer concentrations (D-D), antithrombin activity (AT), and blood platelet counts (PLT). Morphological and biochemical blood parameters were also analysed. Examinations of animals affected by EPC revealed blood coagulation and fibrinolytic disorders, and the noted results (PT 13.7 ±1.06 s, aPTT 23.4 ±1.04 s, TT 15.6 ±0.68 s, FBG 2.2 g/L, D-D 785.4 ±103.05 μg/L, AT 111.1 ±13.51%, PLT 169.30 ±126.31 103/μL) point to a high risk of disseminated intravascular coagulation. The findings indicate that the coagulation parameters of bitches affected by EPC should be analysed before treatment as the noted disorder can significantly complicate therapy and ovariohysterectomy, and endanger the patients' life.

scholarly journals Blood coagulation parameters in fallow deer (Dama dama)

2011 ◽  
Vol 56 (No. 3) ◽  
pp. 119-122 ◽  
Author(s):  
Z. Siroka ◽  
B. Krocilova ◽  
J. Pikula ◽  
H. Bandouchova ◽  
L. Peckova ◽  
...  
Keyword(s):  
Prothrombin Time ◽  
Blood Coagulation ◽  
Fallow Deer ◽  
Thrombin Time ◽  
Wild Animals ◽  
Dama Dama ◽  
Coagulation Parameters ◽  
Clinically Normal ◽  
Viii And Ix ◽  
Physiological Values

There are frequent reports from around the world of wild animals being poisoned with anticoagulants. Granulated baits can result in primary or secondary poisoning of non-target animals. Moreover, there are several diseases including infections that influence haemostasis in wild animals. The present study focused on fallow deer (Dama dama) for which insufficient data on physiological values of coagulation parameters are available. Six parameters of blood coagulation were established in clinically normal fallow deer from a game enclosure in North Moravia (Czech Republic). The fibrinogen content of 1.94 g/l is in agreement with the results obtained by other authors. Factor VIII and IX concentrations amounted to 198.42% and 169.91% of human concentration of these parameters in blood. These have never before been measured for fallow deer, but most animal species have concentrations of these factors higher than humans. Prothrombin time (PT), average activated partial thromboplastin time (APTT), and thrombin time (TT) were assessed as 20.99 s, 33.76 s, and 24.78 s, respectively. Prothrombin time assessed in the present study was longer compared to available data, while APTT is in agreement with the previous data. Thrombin time value is a new piece of information and is comparable with TT values obtained in other ruminants. The possible explanation for the prolonged PT may be the stress associated with yarding and handling the animals which is reported to cause haemorrhages or changes in haemostatic parameters in deer. Interestingly, males had significantly longer clotting times compared to females.

Effects of Human Antithrombin III on Mortality and Blood Coagulation Induced in Rabbits by Endotoxin

1984 ◽  
Vol 51 (02) ◽  
pp. 232-235 ◽  
Author(s):  
D C Triantaphyllopoulos
Keyword(s):  
Blood Coagulation ◽  
Bolus Dose ◽  
Antithrombin Iii ◽  
Coagulation Parameters ◽  
E Coli ◽  
At Iii ◽  
Baseline Values

SummaryTwenty-one rabbits were infused with 20μg/kg/hr of E. coli endotoxin for 6 hr. Eight of the animals were preinjected immediately before the infusion of endotoxin, with a bolus dose of human AT III calculated to increase the antithrombin content of the plasma by about 4 units/ml. All eight animals which were preinjected with AT III survived, while 5 of the 13 control rabbits infused with endotoxin alone died. The changes in coagulation parameters from the baseline values, between the 8 control rabbits which survived and the 8 animals which were preinjected with AT III were compared. The concentration of the preinjected human AT III declined significantly faster (P: <0.01) than that of the native rabbit AT III. AT III prevented the decline of F.XII throughout the infusion of the endotoxin. However, the decline in F.V, fibrinogen, prothrombin and platelets was not affected (P: >0.5) by the injection of AT III.

International Quality Control In Blood Coagulation: Measurement Of Fibrinogen

1979 ◽  
Author(s):  
Jean Thomson ◽  
L Poller ◽  
K Yee
Keyword(s):  
Quality Control ◽  
Blood Coagulation ◽  
Thrombin Time

Fibrinogen estimations were included in four of the collaborative exercises from the above Centre between 1976-1978. More than thirty different techniques were employed by participants in over four hundred laboratories. The thrombin time, fibrinogen titre and turbidity techniques showed poor discrimination between normal and low fibrinogen levels compared with other routine methods e.g. Clauss technique, immunological and clot weight methods.

Study of Haemostasis in Schoenlein-Henoch’s Syndrome

1975 ◽  
Author(s):  
Gy. Boros ◽  
J. Sámik ◽  
Ljubov Gofman ◽  
Judit Nagv ◽  
Gy. Deák ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Blood Coagulation ◽  
Fibrinolytic Activity ◽  
Capillary Permeability ◽  
Thrombin Time ◽  
Consumption Coagulopathy ◽  
Fibrin Deposition ◽  
Coagulation Defect ◽  
Capillary Resistance

Blood coagulation, thrombocyte function and capillary resistance were studied in 21 adult patients with Schoenlein-Henoch’s syndrome. 20 different tests were carried out at different stages of the disease. In 2 cases skin, in 1 mesocolon and in 10 kidneys were examined histologicaly (light microscopy, immunofluorescence, electron microscopy).Capillary permeability was increased at least once in every patient. All patients but 2 were characterized by hypercoagulability. In addition to thrombotic changes, an increase in labile fibrinogen was detected in the serum of 12 patients, and thrombin time was prolonged in 11 patients on 31 occasions. Plasma fibrinolytic activity was increased in 11 patients. Fibrin deposition was demonstrable in the skin of 2 in the mesocolon of 1 and in the kidneys of 7 patients.It is suggested that besides the capillaropathy a coagulation defect, resembling consumption coagulopathy is a characteristic of Schoenlein-Henoch’s syndrome.

scholarly journals Mortality prediction using a novel combination of biomarkers in the first day of sepsis in intensive care units

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Junkun Liu ◽  
Chengwen Bai ◽  
Binbin Li ◽  
Aijun Shan ◽  
Fei Shi ◽  
...  
Keyword(s):  
Septic Shock ◽  
Scoring Systems ◽  
Predictive Performance ◽  
Serum Levels ◽  
International Normalized Ratio ◽  
Individual Performance ◽  
Mortality Prediction ◽  
New Combination ◽  
Thrombin Time ◽  
Survivor Group

AbstractEarly identification of infection severity and organ dysfunction is crucial in improving outcomes of patients with sepsis. We aimed to develop a new combination of blood-based biomarkers that can early predict 28-day mortality in patients with sepsis or septic shock. We enrolled 66 patients with sepsis or septic shock and compared 14 blood-based biomarkers in the first 24 h after ICU admission. The serum levels of interleukin-6 (IL-6) (median 217.6 vs. 4809.0 pg/ml, P = 0.001), lactate (median 2.4 vs. 6.3 mmol/L, P = 0.014), N-terminal prohormone of brain natriuretic peptide (NT-proBNP) (median 1596.5 vs. 32,905.3 ng/ml, P < 0.001), prothrombin time (PT) (median 15.6 vs. 20.1 s, P = 0.030), activated partial thrombin time (APTT) (median 45.1 vs. 59.0 s, P = 0.026), and international normalized ratio (INR) (median 1.3 vs. 1.8, P < 0.001) were significantly lower in the survivor group. IL-6, NT-proBNP, and INR provided the best individual performance in predicting 28-day mortality of patients with sepsis or septic shock. Furthermore, the combination of these three biomarkers achieved better predictive performance (AUC 0.890, P < 0.001) than conventional scoring systems. In summary, the combination of IL-6, NT-proBNP, and INR may serve as a potential predictor of 28-day mortality in critically ill patients with sepsis or septic shock.

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