scholarly journals Efficacy and Cytotoxicity of Binary Mixtures as Root Canal Filling Solvents

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3237
Author(s):  
Inês Ferreira ◽  
Liliana Grenho ◽  
Pedro Gomes ◽  
Ana Cristina Braga ◽  
Maria Helena Fernandes ◽  
...  

Objectives: This study reports the efficacy of two solvent mixtures on the dissolution of gutta-percha and AH Plus sealer, together with the cytotoxicity. Methods: Methyl ethyl ketone (MEK), orange oil, tetrachloroethylene, MEK/tetrachloroethylene (1:1), MEK/orange oil (1:1), and chloroform (control) were tested. Twelve groups (n = 15) of standardized stainless-steel molds filled with softened gutta-percha cones and twelve (n = 15) filled with AH Plus were immersed in the corresponding mixture or individual solvent, in an ultrasonic bath, for either 2 or 5 min. The effect of the solvents was assessed qualitatively by a topographical analysis (scanning electron microscopy) and chemical analysis (Fourier transform infrared spectroscopy), and quantitatively by a weight loss and viscoelastic property (dynamic mechanical analysis) evaluation. The cytotoxicity was assessed on MG63 human osteoblastic cells. Results: The mixtures did not show the formation of new compounds. Both presented significantly higher efficacies compared to their individual solvents, suggesting a synergistic effect. Their dissolution efficacy was similar to that of chloroform, showing high cytocompatibility. Conclusions: The proposed strategy, incorporating ultrasound agitation and profiting from the synergy of adequate solvents, might enhance root canal cleanliness allowing a single-step procedure to dissolve gutta-percha and the sealer remnants, while assuring cytocompatibility with the periapical tissues.

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Soo-Hyuk Lee ◽  
Soram Oh ◽  
Adel Saeed Al-Ghamdi ◽  
Ayman Omar Mandorah ◽  
Kee-Yeon Kum ◽  
...  

The objective of root canal obturation is to achieve a fluid-tight seal. Recently, GuttaFlow bioseal (GB), a root canal sealer composed of polydimethylsiloxane, gutta-percha particles, and bioactive glass ceramics, has been developed, to enhance the sealing ability of root canal filling material. The objective of this study was to assess the sealing ability of GB using a subnanoliter-scaled fluid-flow measuring device and to compare with that of AH Plus (AH). The fluid flow in root canal-filled teeth using either gutta-percha cone (GP) with AH (GAR; n = 10) or GP with GB (GBR; n = 10) and in GP inserted in AH blocks (GA; n = 10) or GP inserted in GB blocks (GB; n = 10) was measured. In addition, fluid flow in sealer blocks of AH (A; n = 10) and GB (B; n = 10), which served as negative controls, was measured. Root canal-filled teeth using GP without any sealer (GR) acted as positive controls (n = 10). The leakage was obtained by calculating the volume of moved water by time (s), after stabilization of the fluid flow was achieved. Statistical analysis was performed using the Kruskal–Wallis test and Mann–Whitney U-test with Bonferroni correction. A p value less than 0.00238 (0.05/21) was considered significantly different. The mean leakages (nL/s) in the groups are as follows: GAR, 0.0958 ± 0.0543; GBR, 0.0223 ± 0.0246; GA, 0.0644 ± 0.0803; GB, 0.0267 ± 0.0182; A, 0.0055 ± 0.0057; B, 0.0052 ± 0.005; and GR, 0.2892 ± 0.3018. The mean leakage in the GBR group was lower than that in the GAR group (p = 0.001), while the mean leakages in the GA and GB groups were not significantly different. GuttaFlow bioseal can be useful in single-cone obturation technique.


2018 ◽  
Vol 17 ◽  
pp. e18924
Author(s):  
Marina de Almeida Salim ◽  
Thais Mageste Duque ◽  
Heloisa Carla Dell Santo Gusman ◽  
Carolina Oliveira de Lima ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
...  

Aim: This study evaluated the effect of a sonic device on the bonding of fiberglass posts cemented with a self-etching adhesive combined with conventional cement or a self-adhesive cement to root dentin. Methods: Forty single-rooted bovine incisors were endodontically prepared using a step-back technique. Gutta-percha and AH Plus sealer were used for root canal filling, combined with a thermoplasticization technique. After 1 week, the post space was prepared and the roots were divided into four groups according to the following factors: adhesive system/resin cement (Ambar/ AllCem Core [FGM] and RelyX U200 [3M]) and application mode (manual or sonic). The posts were cemented and the roots were cut into discs and submitted to push-out bond strength (POBS) test. The failure mode was evaluated using a stereoscope at 25x magnification. The data were analyzed statistically using ANOVA and Tukey’s post-hoc test (α = 0.05). Results: The sonically activated RelyX U200 group was superior to the other groups. RelyX U200 manually applicated showed similar results to those of sonically activated Âmbar/AllCem. These three groups outperformed the Âmbar/AllCem manually applicated group. Adhesive failure between dentin and resin cement was the most predominant pattern. Conclusion: Sonic application of self-etching adhesive and self-adhesive cement improved the POBS of fiber posts to the root canal.


2016 ◽  
Vol 35 (4) ◽  
pp. 644-650 ◽  
Author(s):  
Wonkyung JHO ◽  
Jeong-Won PARK ◽  
Euiseong KIM ◽  
Minju SONG ◽  
Deok-Gyu SEO ◽  
...  

2019 ◽  
Vol 890 ◽  
pp. 275-282
Author(s):  
Isabel Vasconcelos ◽  
Mário Rito Pereira ◽  
António Ginjeira ◽  
Margarida C. Franco ◽  
Pedro Morouço ◽  
...  

This pilot study aims to define a protocol for optimizing the micro-computed tomography (micro-CT) settings to evaluate in the future research the root canal filling in oval shaped canals. Thirty distal canals of mandibular molars were used. After preparation of the canals, the roots were randomly divided into five groups. The canals were filled with two types of sealers (Endosequence BC Sealer or AH Plus) and two types of gutta percha (Protaper Next cones and Endosequence BC cones), using thermal obturation in comparison with a single cone obturation technique. Each specimen was scanned three times using a micro-CT device at a resolution of 30,1 μm. The first scan was done for selecting the specimen according the inclusion criteria, the second one post-instrumentation and the last micro-CT scan after obturation the specimens. For the present study, the images were only evaluated with qualitative criteria and the settings for acquisition, reconstruction and analysis of micro-CT images were tested.


2015 ◽  
Vol 42 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Tugba Bezgin ◽  
Betul Memiş Ozgul ◽  
Volkan Arikan ◽  
Saziye Sari

2021 ◽  
Vol 10 (6) ◽  
pp. 1266
Author(s):  
Vicente Faus-Llácer ◽  
Rubén Pérez ◽  
Ignacio Faus-Matoses ◽  
Celia Ruiz-Sánchez ◽  
Álvaro Zubizarreta-Macho ◽  
...  

The present study aims to evaluate the effectiveness of an XP-endo non-surgical root canal re-treatment system in removing both GuttaCore and Thermafil gutta-percha carrier-based root canal filling materials from straight root canal systems using micro-computed tomography (micro-CT) analysis. The study was performed on 20 single-rooted upper teeth, which were randomly allocated into the following study groups: Group A, Thermafil and AH Plus sealer (n = 10); Group B, GuttaCore and AH Plus sealer (n = 10). Before and after the non-surgical root canal re-treatment procedure, the samples were submitted for a micro-CT analysis. The volume of the root canal filling material (mm3), the volume of the remaining root canal filling material (mm3) and the time (minutes) needed to remove the root canal filling material were also recorded. Student’s t-test was used to analyze the results. No statistically significant differences were found between the volume of the remaining root canal filling material in the GuttaCore and Thermafil root canal filling systems at the coronal third (p = 0.782), middle third (p = 0.838) or apical third (p = 0.882) of the straight root canal systems; however, the GuttaCore required a statistically significant (p = 0.037) shorter amount of time (4.72 ± 0.76 min) to be removed than the Thermafil carrier-based root canal filling material (5.92 ± 1.42 min). The XP-endo Finisher non-surgical endodontic re-treatment system removes both GuttaCore and Thermafil gutta-percha carrier-based root canal filling materials from straight root canal systems, although removal of the GuttaCore gutta-percha carrier-based root canal filling material required less time.


2019 ◽  
Vol 1 (2) ◽  
pp. 55
Author(s):  
Aryadi Subrata ◽  
Anastasia Elsa Prahasti ◽  
Bernard Ongki Iskandar

Introduction: Root canal treatment is done to maintain the teeth to last as long as possible in the oral cavity. This can be done with three main stages, such as biomechanical preparation, sterilization and root canal filling. The hermetic seal of root canal filling has an important role in the success of root canal treatment. Objective: The aim of this in-vitro study is to compare the microbial leakage of root canals filled with RealSeal sealer/Resilon Points and AH Plus sealer/conventional gutta-percha points using single cone obturation technique and warm vertical compaction technique. Methods: Thirty-two extracted human mandibular premolars with single canals were decoronated to a standardized root length of 15 mm and prepared using crown-down technique to a master apical file size 30/.09. Teeth were divided into four experimental groups (n = 7 each group) and controls (n = 1 each group). Teeth in the first group were obturated using single master gutta percha cone and AH Plus sealer and second group were obturated with single Resilon gutta percha cone using RealSeal sealer. Third experimental group was filled with warm vertical condensed gutta-percha using AH Plus sealer and fourth group was filled with warm vertical condensed Resilon gutta-percha using RealSeal sealer. The coronal chamber of each sample were inoculated with Enterococcus faecalis. Results: There were no significant differences between the four experimental groups (P = 0.182). The differences occurred only in the survival time from each group. Group 4 (Warm Vertical Condensation - RealSeal) has the lowest leakage rate among other groups and the fastest leakage occurred in group 1 (Single Cone - AH Plus). Conclusion: The single-cone techniques does not insure durable apical seal against bacterial leakage. Warm vertical compaction technique using Resilon gutta-percha and RealSeal sealer appears to be more effective in minimizing bacterial leakage than gutta-percha and AH Plus sealer.


2010 ◽  
Vol 138 (5-6) ◽  
pp. 287-291 ◽  
Author(s):  
Irena Melih ◽  
Ankica Jakovljevic ◽  
Milica Popovic ◽  
Dragana Pesic

Introduction Root canal filling materials are intended to prevent microleakage and passing of microorganisms and their toxins along the root canal space and into the periradicular tissues. Objective Objective of this in vitro study was to evaluate and compare apical leakage of root canals obturated using a modified warm vertical condensation technique and different materials. Methods Sixty-five extracted single-root teeth were prepared according to the crown-down/ step-back technique. Each canal was rinsed with 3% NaOCl during and after the preparation. The teeth were divided into 3 groups. Every group was obturated by the same technique, but with different materials: RealSeal system (SybronEndo), gutta-percha with AH Plus (Dentsply Maillefer) and gutta-percha with Acroseal sealer (Septodont). The remaining 20 teeth were in the control group. The teeth were immersed in 1% methylene blue for 72 hours. After that period, the roots were split longitudinally, and dye penetration was measured using a stereomicroscope. Results Dye penetration occurred in all groups. The least dye penetration occurred in the RealSeal group (0.33?}0.29 mm), while the highest dye penetration occurred in the Acroseal group (1.11?}0.52 mm). According to the Student's t-test, Acroseal showed significantly more leakage (p<0.001) than RealSeal and AH Plus materials. The microleakage of RealSeal system was not significantly different (p>0.05) from that of AH Plus sealer with gutta-percha. Conclusion The results suggested that new material Resilon (RealSeal) and conventional combination AH Plus with gutta-percha had the least apical dye penetration and provided the best sealing.


2019 ◽  
Vol 2 (1) ◽  
pp. 21
Author(s):  
Aryadi Subrata ◽  
Anastasia Elsa Prahasti ◽  
Bernard Ongki Iskandar

  Introduction: Root canal treatment is done to maintain the teeth to last as long as possible in the oral cavity. This can be done with three main stages, such as biomechanical preparation, sterilization and root canal filling. The hermetic seal of root canal filling has an important role in the success of root canal treatment.   Objective: The aim of this in-vitro study is to compare the microbial leakage of root canals filled with RealSeal sealer/Resilon Points and AH Plus sealer/conventional gutta-percha points using single cone obturation technique and warm vertical compaction technique.   Methods: Thirty-two extracted human mandibular premolars with single canals were decoronated to a standardized root length of 15 mm and prepared using crown-down technique to a master apical file size 30/.09. Teeth were divided into four experimental groups (n = 7 each group) and controls (n = 1 each group). Teeth in the first group were obturated using single master gutta percha cone and AH Plus sealer and second group were obturated with single Resilon gutta percha cone using RealSeal sealer. Third experimental group was filled with warm vertical condensed gutta-percha using AH Plus sealer and fourth group was filled with warm vertical condensed Resilon gutta-percha using RealSeal sealer. The coronal chamber of each sample were inoculated with Enterococcus faecalis.   Results: There were no significant differences between the four experimental groups (P = 0.182). The differences occurred only in the survival time from each group. Group 4 (Warm Vertical Condensation - RealSeal) has the lowest leakage rate among other groups and the fastest leakage occurred in group 1 (Single Cone - AH Plus).   Conclusion: The single-cone techniques does not insure durable apical seal against bacterial leakage. Warm vertical compaction technique using Resilon gutta-percha and RealSeal sealer appears to be more effective in minimizing bacterial leakage than gutta-percha and AH Plus sealer.


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