scholarly journals Influence of Long-Term Storage on the Caking Properties Determined in Punch Test and Fungal Contamination of Potato Starch and Wheat Flour

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 331
Author(s):  
Justyna Wajs ◽  
Jacek Panek ◽  
Magdalena Frąc ◽  
Mateusz Stasiak
Keyword(s):  
Wheat Flour ◽  
Storage Time ◽  
Potato Starch ◽  
Materials Testing ◽  
Fungal Contamination ◽  
Powdered Food ◽  
Punch Test ◽  
Long Term Storage ◽  
Term Storage

The presented results are an attempt to identify the changes taking place during a punch test experiment and the development of fungal impurities of powdered food materials over long-term storage at 75% RH. The potato starch and wheat flour market has a large share of the global production of bulk materials. The growing interest in powdered food materials requires additional production expenditure. This is associated with an increase in storage time of the discussed product and providing it with the appropriate conditions. The samples of potato starch and wheat flour were stored in perforated containers in a climatic chamber at 75% humidity and 21 °C for five months and then samples were measured by a punch test in a Lloyd LRX materials testing machine. The graphs obtained in the potato starch punch test differed significantly from wheat flour. The thickening of potato starch was observed in the form of layers, while potato starch was uniformly thickened throughout the experiment. The conditions of 75% humidity and 21 °C can be described as the beginning of the caking process. In potato starch, linear sections were observed, which changed the length of their storage time and, additionally, was correlated with the appearance of fungal contamination. These results may suggest the influence of fungi on the phenomenon of bulk material caking.

scholarly journals 3′ MACE RNA-sequencing allows for transcriptome profiling in human tissue samples after long-term storage

2020 ◽  
Vol 100 (10) ◽  
pp. 1345-1355 ◽  
Author(s):  
Stefaniya Boneva ◽  
Anja Schlecht ◽  
Daniel Böhringer ◽  
Hans Mittelviefhaus ◽  
Thomas Reinhard ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Storage Time ◽  
Transcriptome Profiling ◽  
Rna Seq ◽  
Fresh Tissue ◽  
Long Term Storage ◽  
Ffpe Samples ◽  
The Impact ◽  
Term Storage

Abstract This study aims to compare the potential of standard RNA-sequencing (RNA-Seq) and 3′ massive analysis of c-DNA ends (MACE) RNA-sequencing for the analysis of fresh tissue and describes transcriptome profiling of formalin-fixed paraffin-embedded (FFPE) archival human samples by MACE. To compare MACE to standard RNA-Seq on fresh tissue, four healthy conjunctiva from four subjects were collected during vitreoretinal surgery, halved and immediately transferred to RNA lysis buffer without prior fixation and then processed for either standard RNA-Seq or MACE RNA-Seq analysis. To assess the impact of FFPE preparation on MACE, a third part was fixed in formalin and processed for paraffin embedding, and its transcriptional profile was compared with the unfixed specimens analyzed by MACE. To investigate the impact of FFPE storage time on MACE results, 24 FFPE-treated conjunctival samples from 24 patients were analyzed as well. Nineteen thousand six hundred fifty-nine transcribed genes were detected by both MACE and standard RNA-Seq on fresh tissue, while 3251 and 2213 transcripts were identified explicitly by MACE or RNA-Seq, respectively. Standard RNA-Seq tended to yield longer detected transcripts more often than MACE technology despite normalization, indicating that the MACE technology is less susceptible to a length bias. FFPE processing revealed negligible effects on MACE sequencing results. Several quality-control measurements showed that long-term storage in paraffin did not decrease the diversity of MACE libraries. We noted a nonlinear relation between storage time and the number of raw reads with an accelerated decrease within the first 1000 days in paraffin, while the numbers remained relatively stable in older samples. Interestingly, the number of transcribed genes detected was independent on FFPE storage time. RNA of sufficient quality and quantity can be extracted from FFPE samples to obtain comprehensive transcriptome profiling using MACE technology. We thus present MACE as a novel opportunity for utilizing FFPE samples stored in histological archives.

scholarly journals Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations

EBioMedicine ◽  
2016 ◽  
Vol 12 ◽  
pp. 309-314 ◽  
Author(s):  
Stefan Enroth ◽  
Göran Hallmans ◽  
Kjell Grankvist ◽  
Ulf Gyllensten
Keyword(s):  
Plasma Protein ◽  
Storage Time ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Stability of Folate and Vitamin B12in Human Serum after Long-Term Storage: A Follow-Up after 13 Years

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Eugène H. J. M. Jansen ◽  
Piet K. Beekhof
Keyword(s):  
Human Serum ◽  
Storage Time ◽  
Vitamin B ◽  
Serum Samples ◽  
Long Term Storage ◽  
Nutrition Research ◽  
The Stability ◽  
Term Storage

In epidemiological and nutrition research, it is very important to evaluate the stability of biomarkers as function of both storage time and temperature. In this study, the stability of folate and vitamin B12in human serum samples has been tested after long-term storage at −80°C up to 13 years. Serum samples of 16 individuals were used in this study. The concentration of folate and vitamin B12has been determined att=0and at 1, 8, and 13 years after storage at −80°C. The folate concentrations in serum samples remained stable at −80°C. The concentration of vitamin B12was decreasing during the time of the study to about 50%. The correlation of the folate and also of the vitamin B12concentrations in the stored samples compared with the starting values was still good. Therefore, although the concentration of vitamin B12decreased upon storage, reliable comparative analyses can still be performed.

Long-term storage stability of selected potato starch – Non-starchy hydrocolloid binary gels

2013 ◽  
Vol 31 (2) ◽  
pp. 270-276 ◽  
Author(s):  
Magdalena Krystyjan ◽  
Greta Adamczyk ◽  
Marek Sikora ◽  
Piotr Tomasik
Keyword(s):  
Storage Stability ◽  
Potato Starch ◽  
Long Term Storage ◽  
Term Storage

The study of the quality of flour obtained from wheat at the stages of long-term storage

2021 ◽  
pp. 449-456
Author(s):  
K.B. Guryeva ◽  
S.L. Beletskiy ◽  
N.A. Khaba
Keyword(s):  
Wheat Flour ◽  
Physicochemical Parameters ◽  
Grain Storage ◽  
Wheat Grain ◽  
Long Term Storage ◽  
Term Storage

This article presents an analysis of the results of the study of flour obtained from batches of wheat grain at the stages of storage. It is shown that during the observed period of grain storage, the quality of the obtained wheat flour was stable and corresponded to the highest grade in terms of physicochemical parameters. Wheat of the 3rd class after 6 years of storage can be used for milling purposes for the production of bakery flour.

Viability and Engraftment of Hematopoietic Progenitor Cells (HPC) After Long Term Cryopreservation: Effect of Diagnosis and % Dimethylsulfoxide (DMSO) Concentration.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3161-3161
Author(s):  
Muthu Kumaran Veeraputhiran ◽  
John Theus ◽  
Gina Pesek ◽  
Bart Barlogie ◽  
Michele H. Cottler-Fox
Keyword(s):  
Storage Time ◽  
Conflicts Of Interest ◽  
Population Data ◽  
T Test ◽  
Hematopoietic Progenitor ◽  
Long Term Storage ◽  
Significant Difference ◽  
Dmso Concentration ◽  
Term Storage

Abstract Abstract 3161 Poster Board III-98 Introduction There are few reports of HPC viability after more than 9 y of storage. It has been suggested that HPC from myeloma patients (pts) may survive storage less well than those from other diseases. The appropriate concentration of DMSO for long term storage is unclear. Our program has collected and stored HPC since November 1989, changing from 5% DMSO to 10% DMSO in August 2000. We present a retrospective analysis of viability by diagnosis and DMSO concentration in pts who underwent autologous transplant using HPC after long-term storage (up to 17.8 y). Materials and Methods HPC were harvested and preserved using a controlled rate freezer and 5% or 10% DMSO, then stored in liquid nitrogen. Viability was tested by flow cytometry using unwashed samples and looking at propidium iodide or 7-AAD uptake within the CD34+ population. Data from 242 samples were analyzed (224 myeloma pts and 18 other diagnoses): 100 consecutively thawed samples with storage time <1 y (all 10% DMSO), 100 consecutive samples stored from 5-9 y ( 7 samples, 10% and 93 samples, 5% DMSO), and all samples stored and used for transplant after >9y (42 samples, 5% DMSO). No statistically significant difference was seen in viability between <1y and 5-9y, so these groups were combined and compared to >9y. White blood cell (WBC) and platelet engraftment were compared for the 2 groups using an unpaired t test. Results Mean viability for the 0-9y and >9 y groups were 91.62% and 92.79% respectively, with no statistically significant difference in viability between groups (p=0.28). Using a 2-tailed t test, no difference was seen in viability based on diagnosis (p=0.19) or between 5% and 10% DMSO (p= 0.12). No difference was seen in WBC engraftment (p=0.28) or platelet engraftment between groups (p=0.57). Discussion HPC collections remained viable after 17.8 years, the longest time reported for use after storage to date. No difference in viability was seen with 5% or 10% DMSO, or between myeloma and other diseases. Storage time did not affect engraftment. Disclosures No relevant conflicts of interest to declare.

Effect of long-term storage conditions on wheat flour and bread baking properties

2021 ◽  
Vol 346 ◽  
pp. 128902
Author(s):  
Eloïse Lancelot ◽  
Joran Fontaine ◽  
Joëlle Grua-Priol ◽  
Alain Le-Bail
Keyword(s):  
Wheat Flour ◽  
Storage Conditions ◽  
Long Term Storage ◽  
Term Storage

scholarly journals 1-Methylcyclopropene postharvest treatment and their effect on apple quality during long-term storage time

2014 ◽  
Vol 239 (4) ◽  
pp. 603-612 ◽  
Author(s):  
Joanna Kolniak-Ostek ◽  
Aneta Wojdyło ◽  
Jarosław Markowski ◽  
Karolina Siucińska
Keyword(s):  
Storage Time ◽  
Long Term Storage ◽  
Postharvest Treatment ◽  
Apple Quality ◽  
Term Storage

Physical Property Changes in Commercial Natural Rubbers during Long Term Storage

2006 ◽  
Vol 79 (1) ◽  
pp. 72-81 ◽  
Author(s):  
Jintana Yunyongwattanakorn ◽  
Jitladda T. Sakdapipanich
Keyword(s):  
Physical Properties ◽  
Storage Time ◽  
Physical Property ◽  
Gel Content ◽  
Long Term Storage ◽  
Property Changes ◽  
Term Storage ◽  
Clear Relation ◽  
Long Storage

Abstract The physical properties during long term storage of selected commercial Standard Thai Rubbers (STR), i.e. STR XL, STR 5L and STR CV60 as high-graded natural rubber (NR), were investigated. Sections of commercial NR were subjected to examination after storage-hardening. STR 5L showed clearly an increase in Mooney viscosity (VR), Mooney relaxtion (MR30), gel content and initial plasticity (P0), higher than that of STR XL. This result suggests that STR 5L exhibits the highest inconsistency in physical properties. The increasing viscosity and gel content of STR 5L and STR XL samples suggest the occurrence of a crosslink structure during storage. STR CV60, known as a viscosity-stabilized NR sample, also showed an increase in VR, gel content, P0, and high PRI value during long storage. These findings indicate that storage-hardening occurred in the rubber samples even after carefully controlled production procedures. As for the different sections of samples, there was no clear relation with gel content with respect to storage time, indicating that depth or positions of specimens in a certain rubber bale did not affect the storage-hardening phenomenon.

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