scholarly journals In Vitro Evaluation of Structural Factors Favouring Bacterial Adhesion on Orthodontic Adhesive Resins

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2485
Author(s):  
Roberta Condò ◽  
Gianluca Mampieri ◽  
Guido Pasquantonio ◽  
Aldo Giancotti ◽  
Paola Pirelli ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Composite Resins ◽  
Structural Factors ◽  
Internal Structures ◽  
Orthodontic Materials ◽  
Combine Information

Bacterial adhesion to the surface of orthodontic materials is an important step in the formation and proliferation of plaque bacteria, which is responsible for enamel demineralization and periodontium pathologies. With the intent of investigating if adhesive resins used for bracket bonding are prone to bacteria colonization, the surface roughness of these materials has been analyzed, combining information with a novel methodology to observe the internal structures of orthodontic composites. Scanning electron microscopy, combined with focus ion bean micromachining and stylus profilometry analyses, were performed to evaluate the compositional factors that can influence specific pivotal properties facilitating the adhesion of bacteria to the surface, such as surface roughness and robustness of three orthodontic adhesive composite resins. To confirm these findings, contact angle measurements and bacteria incubation on resin slide have been performed, evaluating similarities and differences in the final achievement. In particular, the morphological features that determine an increase in the resins surface wettability and influence the bacterial adhesion are the subject of speculation. Finally, the focused ion beam technique has been proposed as a valuable tool to combine information coming from surface roughness with specific the internal structures of the polymers.

scholarly journals In vitro Evaluation of Structural Factors Favouring Bacterial Adhesion on Orthodontic Adhesive Resins

2021 ◽  
Author(s):  
Roberta Condò ◽  
Gianluca Mampieri ◽  
Guido Pasquantonio ◽  
Aldo Giancotti ◽  
Paola Pirelli ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Structural Factors ◽  
Adhesive Material ◽  
Internal Structures ◽  
Structural Nature ◽  
Beam Technique

Bacterial adhesion to the surface of the adhesive material is an important step in the formation of plaque and enamel demineralization. In order to correlate the material composition to the specific surface roughness of the resin and to the probable more favourable adhesion of bacteria, scanning electron microscopy, combined with focus ion bean micromachining, together with stylus profilometry analysis have been in vitro performed to reveal the structural nature of three orthodontic adhesive resins used for bracket bonding and, above all, to understand how compositional factors can influence specific pivotal properties such as material’s surface roughness and robustness. In particular, we speculated about the morphological features that determine an increase in the bacterial adhesion and we proposed focused ion beam technique as a valuable tool to compare the internal structures of the polymers and to determine the peculiar mechanical properties of the examined adhesive resins.

In Vitro Impact of a Glaze/ Composite Resin Sealant on the Surface Roughness and Bacterial Adhesion of One Microhybrid and Three Nanofilled Composite Resins

2013 ◽  
Vol 3 (2) ◽  
pp. 130-144
Author(s):  
Salah Hasab Mahmoud ◽  
Naglaa Rezk El-Kholany ◽  
Mohammed EL-Awady Grawish ◽  
Salwa Abd El-Raof El- Negoly
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Composite Resin ◽  
Composite Resins ◽  
Resin Sealant

Effect of pH Cycling Followed by Simulated Toothbrushing on the Surface Roughness and Bacterial Adhesion of Bulk-fill Composite Resins

2020 ◽  
Vol 45 (2) ◽  
pp. 209-218 ◽  
Author(s):  
DC Somacal ◽  
FB Manfroi ◽  
MSG Monteiro ◽  
SD Oliveira ◽  
HR Bittencourt ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Composite Resin ◽  
Composite Resins ◽  
Sem Images ◽  
Effect Of Ph ◽  
Group 4 ◽  
Ph Cycling ◽  
Group 2

SUMMARY The aim was to evaluate, in vitro, quantitatively and qualitatively, the effect of pH cycling and simulated toothbrushing on surface roughness (Ra) and bacterial adhesion (Cn) of bulk-fill composite resins. Thirty specimens of each composite resin, 5 mm wide and 4 mm high, were obtained: group 1 (control): Filtek Z250 (Z250); group 2: Filtek Bulk-Fill (FTK); group 3: Tetric N-Ceram Bulk-Fill (TTC); and group 4: Aura Bulk-Fill (AUR). After 24 hours, the specimens were polished and then alternated with demineralization/remineralization solutions for 15 cycles of 24 hours each at 37°C. Then the specimens were submitted to simulated toothbrushing. The Ra and Cn measurements were quantitatively analyzed in three stages: after polishing (Ra0 and Cn0), after pH cycling (Ra1 and Cn1), and after simulated toothbrushing (Ra2 and Cn2). The Ra values were submitted to two-way analysis of variance, followed by the Tukey test (α=0.05). The Kruskal-Wallis test, followed by multiple comparisons, was applied for Cn analysis. Surface topography and bacterial adhesion were observed by scanning electron microscopy (SEM). Z250, FTK, and TTC showed no significant change in Ra regardless of the treatment performed; AUR obtained increased Ra at Ra2 (p<0.05). FTK differed from the others at Cn0 and Cn1 (p<0.05). At Cn2, there was no difference among the composite resins. SEM images showed the exposure of fillers and microcavities at Ra1 and Ra2. There was greater bacterial adhesion at Cn1 for Z250 and FTK. It was concluded that the pH cycling caused surface degradation of all composite resins, which was potentiated by simulated toothbrushing. However, only AUR presented an increased Ra. Bacterial adhesion occurred on all composite resins after pH cycling; however, after simulated toothbrushing, adhesion of dispersed bacteria was similar for all the composite resin groups.

Surface roughness of a Co-less WC micro-die fabricated by focused-ion-beam machining

2004 ◽  
Vol 84 (3) ◽  
pp. 149-155 ◽  
Author(s):  
Hiroyuki Hosokawa ◽  
Koji Shimojima ◽  
Mamoru Mabuchi
Keyword(s):  
Surface Roughness ◽  
Focused Ion Beam ◽  
Ion Beam

scholarly journals FIB-SEM Three-Dimensional Tomography for Characterization of Carbon-Based Materials

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Nan Nan ◽  
Jingxin Wang
Keyword(s):  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
3D Tomography ◽  
Tomography System ◽  
Internal Structures ◽  
Potential Applications ◽  
Scanning Electron ◽  
Carbon Based

A review on the recent advances of the three-dimensional (3D) characterization of carbon-based materials was conducted by focused ion beam-scanning electron microscope (FIB-SEM) tomography. Current studies and further potential applications of the FIB-SEM 3D tomography technique for carbon-based materials were discussed. The goal of this paper is to highlight the advances of FIB-SEM 3D reconstruction to reveal the high and accurate resolution of internal structures of carbon-based materials and provide suggestions for the adoption and improvement of the FIB-SEM tomography system for a broad carbon-based research to achieve the best examination performances and enhance the development of innovative carbon-based materials.

scholarly journals The structure of the COPI coat determined within the cell

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Yury S Bykov ◽  
Miroslava Schaffer ◽  
Svetlana O Dodonova ◽  
Sahradha Albert ◽  
Jürgen M Plitzko ◽  
...  
Keyword(s):  
Focused Ion Beam ◽  
Structural Model ◽  
Electron Tomography ◽  
Ion Beam ◽  
Membrane Thickness ◽  
In Vitro Reconstitution ◽  
Cryo Electron Tomography ◽  
Subtomogram Averaging

COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively studied with in vitro reconstitution systems using purified components. Previously we have determined a complete structural model of the in vitro reconstituted COPI coat (Dodonova et al., 2017). Here, we applied cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determine the native structure of the COPI coat within vitrified Chlamydomonas reinhardtii cells. The native algal structure resembles the in vitro mammalian structure, but additionally reveals cargo bound beneath β’–COP. We find that all coat components disassemble simultaneously and relatively rapidly after budding. Structural analysis in situ, maintaining Golgi topology, shows that vesicles change their size, membrane thickness, and cargo content as they progress from cis to trans, but the structure of the coat machinery remains constant.

Bacterial adhesion on direct and indirect dental restorative composite resins: An in vitro study on a natural biofilm

2017 ◽  
Vol 117 (5) ◽  
pp. 669-676 ◽  
Author(s):  
Giacomo Derchi ◽  
Michele Vano ◽  
Antonio Barone ◽  
Ugo Covani ◽  
Alberto Diaspro ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
In Vitro Study ◽  
Composite Resins ◽  
Vitro Study ◽  
Dental Restorative

scholarly journals Effects of Toothbrush Hardness on in vitro Wear and Roughness of Composite Resins

2013 ◽  
Vol 14 (6) ◽  
pp. 1137-1144 ◽  
Author(s):  
Hideaki Kyoizumi ◽  
Junji Yamada ◽  
Toshimitsu Suzuki ◽  
Masafumi Kanehira ◽  
Werner J Finger ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Composite Resin ◽  
Testing Machine ◽  
Composite Resins ◽  
Minor Effect ◽  
Wear Depth ◽  
Glass Fillers ◽  
Glass Filler ◽  
Representative Samples

ABSTRACT Aim To investigate and compare the effects of toothbrushes with different hardness on abrasion and surface roughness of composite resins. Materials and methods Toothbrushes (DENT. EX Slimhead II 33, Lion Dental Products Co. Ltd., Tokyo, Japan) marked as soft, medium and hard, were used to brush 10 beam-shaped specimens of each of three composites resins (Venus [VEN], Venus Diamond [VED] and Venus Pearl [VEP]; HeraeusKulzer) with standardized calcium carbonate slurry in a multistation testing machine (2N load, 60 Hz). After each of five cycles with 10k brushing strokes the wear depth and surface roughness of the specimens were determined. After completion of 50k strokes representative samples were inspected by SEM. Data were treated with ANOVA and regression analyses (p < 0.05). Results Abrasion of the composite resins increased linearly with increasing number of brushing cycles (r2 > 0.9). Highest wear was recorded for VEN, lowest for VED. Hard brushes produced significantly higher wear on VEN and VEP, whereas no difference in wear by toothbrush type was detected for VED. Significantly highest surface roughness was found on VED specimens (Ra > 1.5 μm), the lowest one on VEN (Ra < 0.3 μm). VEN specimens showed increased numbers of pinhole defects when brushed with hard toothbrushes, surfaces of VEP were uniformly abraded without level differences between the prepolymerized fillers and the glass filler-loaded matrix, VED showed large glass fillers protruding over the main filler-loaded matrix portion under each condition. Conclusion Abrasion and surface roughness of composite resins produced by toothbrushing with dentifrice depend mainly on the type of restorative resin. Hardness grades of toothbrushes have minor effects only on abrasion and surface roughness of composite resins. No relationship was found between abrasion and surface roughness. Clinical significance The grade of the toothbrush used has minor effect on wear, texture and roughness of the composite resin. How to cite this article Kyoizumi H, Yamada J, Suzuki T, Kanehira M, Finger WJ, Sasaki K. Effects of Toothbrush Hardness on in vitro Wear and Roughness of Composite Resins. J Contemp Dent Pract 2013;14(6):1137-1144.

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