scholarly journals Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

Marine Drugs ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 119
Author(s):  
Wenjie Zhang ◽  
Ling Wei ◽  
Rong Xu ◽  
Guodong Lin ◽  
Huijie Xin ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Amyloliquefaciens ◽  
Fermentation Conditions ◽  
Antibacterial Materials ◽  
Material Production ◽  
Chiloscyllium Plagiosum ◽  
Antibacterial Material ◽  
Burman Design ◽  
Bamboo Shark ◽  
Plackett Burman Design

Bacillus amyloliquefaciens-9 (GBacillus-9), which is isolated from the intestinal tract of the white-spotted bamboo shark (Chiloscyllium plagiosum), can secrete potential antibacterial materials, such as β-1,3-1,4-glucanase and some antimicrobial peptides. However, the low fermentation production has hindered the development of GBacillus-9 as biological additives. In this study, the Plackett–Burman design and response surface methodology were used to optimize the fermentation conditions in a shake flask to obtain a higher yield and antibacterial activity of GBacillus-9. On the basis of the data from medium screening, M9 medium was selected as the basic medium for fermentation. The data from the single-factor experiment showed that sucrose had the highest antibacterial activity among the 10 carbon sources. The Plackett–Burman design identified sucrose, NH4Cl, and MgSO4 as the major variables altering antibacterial activity. The optimal concentrations of these compounds to enhance antibacterial activity were assessed using the central composite design. Data showed that sucrose, NH4Cl, and MgSO4 had the highest antibacterial activities at concentrations of 64.8, 1.84, and 0.08 g L−1, respectively. The data also showed that the optimal fermentation conditions for the antibacterial material production of GBacillus-9 were as follows: Inoculum volume of 5%, initial pH of 7.0, temperature of 36 °C, rotating speed of 180 rpm, and fermentation time of 10 h. The optimal fermentation medium and conditions achieved to improve the yield of antibacterial materials for GBacillus-9 can enhance the process of developing biological additives derived from GBacillus-9.

Fermentation Conditions Optimization of Anti-Tumor Active Metabolites from Marine Penicillium sp. HGQ6

2011 ◽  
Vol 340 ◽  
pp. 344-350
Author(s):  
Guo Lei ◽  
Wen Cheng Zhu ◽  
Chao Liu
Keyword(s):  
Optimal Conditions ◽  
Inhibition Activity ◽  
Active Metabolites ◽  
Fermentation Conditions ◽  
Burman Design ◽  
Predicted Values ◽  
Initial Medium ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design ◽  
Active Substances

The objectives of this study were to optimize fermentation conditons of anti-tumor active metabolites from marine penicillium sp. HGQ6. Based on the inhibition activity against stomach cancer cell BGC823, firstly, the initial medium was screened, then seven factors including peptone, sucrose, K2HPO4, FeSO4, pH, temperature and agitation were investigated by Plackett-Burman design, and the important variables including pH, temperature and K2HPO4 were optimized by using Response surface methodology (RSM). Experimental results showed that the optimum fermentation conditions were peptone 1%, sucrose 3%, K2HPO4 0.06%, FeSO4 0.001%, pH6.41, temperature 35°C and agitation 180 r·min-1. Under these optimal conditions, the actual inhibition ratio achieved 90.4%, which agreed with the predicted values. This indicated the good feasibility of Plackett-Burman design and RSM in the optimization of fermentation conditions for production of anti-tumor active substances from stain HGQ6.

Optimization of Fermentation Conditions for Cellulase Production by HDZK-BYTF620 (Aureobasidium pullulans) which is from the Gut of Termites

2014 ◽  
Vol 692 ◽  
pp. 167-171
Author(s):  
Ming Jing Sun ◽  
Li Qing Sun ◽  
Shan Shan Wang ◽  
Kai Zhao
Keyword(s):  
Aureobasidium Pullulans ◽  
Fermentation Medium ◽  
Cellulase Production ◽  
Fermentation Conditions ◽  
Steepest Ascent ◽  
Burman Design ◽  
Major Factors ◽  
Initial Medium ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

The components of a fermentation medium were optimized to produce cellulose from HDZK-BYTF620 (Aureobasidium pullulans) which is from the gut of termites and can produce cellulose under liquid state fermentation conditions by response surface methodology. By Plackett-Burman design screened out three major factors of strains HDZK-BYTF620 which affected production of the activity cellulose, immediately, CaCl2, MgSO4and CMC-Na. The fermentation conditions were optimized with Box-Behnken experiment and the largest regional of the activity of cellulase was approach with the steepest ascent experiment. The optimum composition of medium addition of CaCl2, MgSO4and CMC-Na was 0.77 g/L, 0.41 g/L and 21.88 g/L, respectively. Under the optimum fermentation medium conditions, the activity of cellulase was (598.00±2.56)×10-3IU/mL, increased 5.0 % to that of using initial medium.

scholarly journals From the Complex Process of Gold Ions Recovery in New Antimicrobial Product

2019 ◽  
Vol 70 (3) ◽  
pp. 1080-1084
Author(s):  
Maria Mihailescu ◽  
Petru Negrea ◽  
Narcis Duteanu ◽  
Adina Negrea ◽  
Mihaela Ciopec ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
High Resistance ◽  
Antibacterial Agents ◽  
Adsorption Process ◽  
Toxicity Tests ◽  
Antibacterial Materials ◽  
Good Antibacterial Activity ◽  
Complex Process ◽  
Domestic Use ◽  
Antibacterial Material

Production of new antibacterial materials is really important due to high resistance bacteria at classical antibiotics. In present study,using the adsorption process of gold ions loaded onto Amberlite XAD7-L-glutamic acid, antibacterial material wasproduced.The antibacterial activity of produced material was studied. In order to test this activity, frstly it was necessary to know the microbiological behaviour of Au(III), by running several toxicity tests. Synthesized material show good antibacterial activity against a broad spectrum of bacteria collected form BegaRiver. These studies were performed in order to find a new application of such materials as paints used as antibacterial agents in hospitals and for domestic use.

Medium Optimization for the Antibacterial Substances Production from Brevibacillus brevis XDH Using Response Surface Methodology

2011 ◽  
Vol 345 ◽  
pp. 355-360 ◽  
Author(s):  
Zhen Song ◽  
Xun Li Liu ◽  
Chang Xu Lu ◽  
Jian Yu ◽  
Rui Cheng Ju ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Antibacterial Activity ◽  
Response Surface ◽  
Medium Optimization ◽  
Fermentation Medium ◽  
Steepest Ascent ◽  
High Antibacterial Activity ◽  
Burman Design ◽  
Brevibacillus Brevis ◽  
Plackett Burman Design

Brevibacillus Brevis XDH is an antagonistic bacterium with high antibacterial activity against many pathogens. Fermentation medium for the production of the antibacterial substances was statistically optimized by Plackett-Burman design, steepest ascent experiments and response surface methodology. The optimized medium was composed of (g/L): glucose 15.6, soybean meal 28.4, starch 6, CaCO3 2 and MgSO4 4. After optimization, the production of antibacterial metabolites increased more than 70% than that of control.

Assessment of liquid chromatographic method robustness by use of Plackett-Burman design

2010 ◽  
Vol 22 (2) ◽  
pp. 281-296 ◽  
Author(s):  
M. Mašković ◽  
B. Jančić-Stojanović ◽  
A. Malenović ◽  
D. Ivanović ◽  
M. Medenica
Keyword(s):  
Chromatographic Method ◽  
Liquid Chromatographic Method ◽  
Burman Design ◽  
Liquid Chromatographic ◽  
Plackett Burman Design

Using a Plackett–Burman design to maximise yield of rosemary essential oil by distillation

2021 ◽  
Vol 166 ◽  
pp. 113488
Author(s):  
Agnese Spadi ◽  
Giulia Angeloni ◽  
Lorenzo Guerrini ◽  
Ferdinando Corti ◽  
Marco Michelozzi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Rosemary Essential Oil

scholarly journals Curcumin-loaded polyvinyl butyral film with antibacterial activity

e-Polymers ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 673-681
Author(s):  
Yanchao Qiao ◽  
Lijie Duan
Keyword(s):  
Mechanical Property ◽  
Thermal Stability ◽  
Antibacterial Activity ◽  
Polyvinyl Butyral ◽  
Counting Method ◽  
E Coli ◽  
Antibacterial Materials ◽  
Good Antibacterial Activity ◽  
Colony Counting ◽  
Widespread Interest

AbstractAntibacterial materials have found widespread interest in different fields nowadays. In this study, curcumin (Cur) was incorporated into the polyvinyl butyral (PVB) matrix by dissolving in ethanol for improving the functional properties of a pure PVB film. We found that Cur was uniformly dispersed in the PVB matrix, which showed good compatibility. Moreover, the incorporation of Cur could also improve thermal stability, hydrophilicity, and mechanical property. The UV-vis spectra of the PVB–Cur film demonstrated that the film could block ultraviolet radiation. Subsequently, the antibacterial activity of the PVB–Cur film was measured by the colony-counting method against S. aureus and E. coli. The results showed that the PVB–Cur film exhibited good antibacterial activity. Therefore, the PVB–Cur film was considered as a promising material for food and medical packaging applications.

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