scholarly journals Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina

Marine Drugs ◽  
2021 ◽  
Vol 19 (11) ◽  
pp. 595
Author(s):  
Yanan Xu ◽  
Patricia J. Harvey
Keyword(s):  
Dunaliella Salina ◽  
Uv Light ◽  
Red Light ◽  
General Effect ◽  
Health Food ◽  
Food Sector ◽  
Cosmetic Industry ◽  
Green Microalga ◽  
Massive Accumulation ◽  
Different Levels

Phytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The halotolerant green microalga Dunaliella salina is one of the richest sources of natural carotenoids. We have previously investigated the over-production of phytoene in D. salina after cultivation with the well-characterised mitosis inhibitor, chlorpropham. In this study, 15 herbicides with different modes of action were tested for their potential to promote phytoene accumulation. All herbicides showed different levels of capabilities to support phytoene over-production in D. salina. Most significantly, the two mitosis inhibitors tested in this study, propyzamide and chlorpropham, showed similar capacities to support the over-production of phytoene by D. salina cultures as phytoene desaturase inhibitors. The cellular content of phytoene increased by over 10-fold within 48 h of treatment with the mitosis inhibitors compared to untreated cultures. Results indicate a general effect of mitosis inhibitors on phytoene accumulation in D. salina. Furthermore, red light was found to significantly enhance the phytoene yield when used in combination with effective inhibitor treatments. Red light can be applied to maximize the production of phytoene from D. salina.

scholarly journals Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina

2021 ◽  
Author(s):  
Yanan Xu ◽  
Patricia J Harvey
Keyword(s):  
Dunaliella Salina ◽  
Uv Light ◽  
Red Light ◽  
General Effect ◽  
Health Food ◽  
Food Sector ◽  
Cosmetic Industry ◽  
Green Microalga ◽  
Massive Accumulation ◽  
Different Levels

Phytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The halotolerant green microalga Dunaliella salina is one of the richest sources of natural carotenoids. We have previously investigated over-production of phytoene in D. salina after cultivation with the well-characterised mitosis inhibitor, chlorpropham. In this study, 15 herbicides with different modes of action were tested for their potential to promote phytoene accumulation. All herbicides showed different levels of capabilities to support phytoene over-production in D. salina. Most significantly, the two mitosis inhibitors tested in this study, propyzamide and chlorpropham, showed similar capacities to support over-production of phytoene by D. salina cultures, as phytoene desaturase inhibitors. The cellular content of phytoene increased by over 10-fold within 48 h after treatment with the mitosis inhibitors compared to untreated cultures. Results indicate a general effect of mitosis inhibitors on phytoene accumulation in D. salina. Furthermore, red light was found to significantly enhance the phytoene yield when used in combination with effective inhibitor treatments. Red light can be applied to maximize the production of phytoene from D. salina.

Persistent Luminescence Cr3+ Doped Spinels and Use as Biomarker for In Vivo Imaging

2014 ◽  
Vol 90 ◽  
pp. 157-165
Author(s):  
Suchinder K. Sharma ◽  
D. Gourier ◽  
B. Viana ◽  
T. Maldiney ◽  
E. Teston ◽  
...  
Keyword(s):  
Uv Light ◽  
Red Light ◽  
Band Gap Energy ◽  
Transparency Window ◽  
Persistent Luminescence ◽  
High Brightness ◽  
Mouse Imaging ◽  
Classical Quantum ◽  
Living Tissues

ZnGa2O4(ZGO) is a normal spinel. When doped with Cr3+ions, ZGO:Cr becomes a high brightness persistent luminescence material with an emission spectrum perfectly matching the transparency window of living tissues. It allowsin vivomouse imaging with a better signal to background ratio than classical quantum dots. The most interesting characteristic of ZGO:Cr lies in the fact that its LLP can be excited with red light, well below its band gap energy and in the transparency window of living tissues. A mechanism based on the trapping of carriers localized around a special type of Cr3+ions namely CrN2can explain this singularity. The antisite defects of the structure are the main responsible traps in the persistent luminescence mechanism. When located around Cr3+ions, they allow, via Cr3+absorption, the storage of not only UV light but also all visible light from the excitation source.

scholarly journals LED and UV Light Utilizing Flavonoids and Limonoids to Investigate the Effect of Post-harvest Light Irradiation

2020 ◽  
Author(s):  
Shengyu Liu
Keyword(s):  
White Light ◽  
Uv Light ◽  
Light Emitting Diode ◽  
Red Light ◽  
Principal Component ◽  
Experimental Period ◽  
Light Irradiation ◽  
Flavonoid Glycosides ◽  
Clear Correlation ◽  
Post Harvest

To investigate the effect of post-harvest light irradiation on the accumulation of flavonoids and limonoids, harvestedNewhall navel oranges were continuously exposed to light-emitting diode (LED) and ultraviolet (UV) light irradiationfor 6 days, and the composition and content of flavonoids and limonoids in the segments were determined usingUPLC-qTOF-MS at 0, 6, and 15 days after harvest. In total, six polymethoxylated flavonoids (PMFs), fiveflavoneO/C-glycosides, seven flavanone-O-glycosides, and three limonoids were identified in the segments. Theaccumulation of these components was altered by light irradiation. Red and blue light resulted in higher levels ofPMFs during exposure periods. The accumulation of PMFs was also significantly induced after white light, UVBand UVC irradiation were removed. Red and UVC irradiation induced the accumulation of flavone and flavanoneglycosides throughout the entire experimental period. Single light induced limonoid accumulation during exposureperiods, but limonoid levels decreased significantly when irradiation was removed. Principal component analysisshowed a clear correlation between PMFs and white light, between flavonoid glycosides and red light and UVC,and between limonoids and UVC. These results suggest that the accumulation of flavonoids and limonoids in citrusis regulated by light irradiation. White light, red light and UVC irradiation might be a good potential method forimproving the nutrition and flavor quality of post-harvest citrus.

scholarly journals Etude De Quelques Caracteristiques Physicochimiques Et Biochimiques De Wavé-fortex Un Complement Alimentaire Naturel Du Gabon

2016 ◽  
Vol 12 (33) ◽  
pp. 508
Author(s):  
Alexis Nicaise Lepengue ◽  
Alain Souza ◽  
Jean Fabrice Yala ◽  
Judicaël Lebamba ◽  
Jacques Mavoungou ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Functional Food ◽  
Dry Matter ◽  
Matter Wave ◽  
Sperm Production ◽  
Health Food ◽  
Nutritional Properties ◽  
Biochemical Characteristics ◽  
Food Powder ◽  
Different Levels

Wavé-fortex is a dietary supplement designed to Gabon, to fight against the problems of malnutrition and undernourishment. It would also contain diuretic properties, purgative, antimicrobial, aphrodisiac, influenza, regulating blood pressure, reducing overweight and stimulating sperm production. This work was carried out to study the physicochemical and biochemical characteristics of this health food. The results revealed that Wavé-fortex was heavily dehydrated and contained 6.27% of crude ash and 11.32% of total gross fibers. The mineral level, Wavé-fortex contains 1.27% of calcium, 0.09% of iron, 0.37% of magnesium, 0.87% of potassium and 0.01% of zinc. Biochemical analyzes showed that this functional food contained 45.69% of carbohydrates, 26.01% of protein and 4.67% of fat. This gives it an energy value of 3.55 kcal / g of dry matter. Wavé-fortex is an acidic product (pH 4.02) with high levels of vitamin A (2.38%), vitamin C (1.93%) and phenolic compounds (11.83%). The different levels of these compounds are capable of validating the various nutritional properties attributed to this food powder.

scholarly journals Stereoisomers of Colourless Carotenoids from the Marine Microalga Dunaliella salina

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1880 ◽  
Author(s):  
Laura Mazzucchi ◽  
Yanan Xu ◽  
Patricia Harvey
Keyword(s):  
Dunaliella Salina ◽  
Light Stress ◽  
Red Light ◽  
Chemical Properties ◽  
Phytoene Desaturase ◽  
Cell Factory ◽  
Geranylgeranyl Diphosphate ◽  
Diverse Range ◽  
Marine Microalga ◽  
Β Carotene

Carotenoids comprise a diverse range of naturally occurring stereoisomers, which differ in their physico-chemical properties. Their biosynthesis begins with phytoene, which is a rarity among carotenoids because it is colourless. Phytoene is sought after as a skin protectant against harmful UV range B (290–320 nm) and C (100–290 nm) light, and as a natural skin-whitening agent and is synthesized from geranylgeranyl diphosphate. Geranylgeranyl diphosphate is catalysed by phytoene synthase and phytoene desaturase to phytoene and phytofluene, respectively. The subsequent steps involve desaturation, isomerisation and cyclisation reactions to form α- and β-carotene stereoisomers, via all-trans lycopene. The marine microalga Dunaliella salina is the richest source of β-carotene, but it can accumulate phytoene and phytofluene as well. In the present study, different analytical tools including High-Performance Liquid Chromatography (HPLC), Ultra-Performance Convergence Chromatography (UPC2-MS) and Nuclear Magnetic Resonance (NMR) were used to characterize and quantify the phytoene isomeric configurations in D. salina in order to explore both the feasibility of D. salina as a cell factory for phytoene production and to gain new insight into the carotenoid synthesis pathway in D. salina. D. salina, similar to tomato, produced predominantly 15-cis phytoene isomer (>98%) and a trace amount of all-trans phytoene (<2%). High light stress, red light stress, or use of a phytoene desaturase inhibitor or a mitotic disrupter herbicide led to the accumulation of 15-cis phytoene but not all-trans phytoene. 9-cis phytoene was not detected in any of the extracts of D. salina biomass. Our main findings suggest that 15-cis phytoene is the most abundant isomer in D. salina and that it is subject to a series of isomerisation and desaturation reactions to form all-trans and 9-cis β-carotene.

scholarly journals Effect of Post-Harvest LED and UV Light Irradiation on the Accumulation of Flavonoids and Limonoids in the Segments of Newhall Navel Oranges (Citrus sinensis Osbeck)

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1755 ◽  
Author(s):  
Shengyu Liu ◽  
Linping Hu ◽  
Dong Jiang ◽  
Wanpeng Xi
Keyword(s):  
White Light ◽  
Uv Light ◽  
Light Emitting Diode ◽  
Red Light ◽  
Principal Component ◽  
Light Irradiation ◽  
Flavonoid Glycosides ◽  
Clear Correlation ◽  
Post Harvest ◽  
Uv Light Irradiation

To investigate the effect of post-harvest light irradiation on the accumulation of flavonoids and limonoids, harvested Newhall navel oranges were continuously exposed to light-emitting diode (LED) and ultraviolet (UV) light irradiation for 6 days, and the composition and content of flavonoids and limonoids in the segments were determined using UPLC-qTOF-MS at 0, 6, and 15 days after harvest. In total, six polymethoxylated flavonoids (PMFs), five flavone-O/C-glycosides, seven flavanone-O-glycosides, and three limonoids were identified in the segments. The accumulation of these components was altered by light irradiation. Red and blue light resulted in higher levels of PMFs during exposure periods. The accumulation of PMFs was also significantly induced after white light, UVB and UVC irradiation were removed. Red and UVC irradiation induced the accumulation of flavone and flavanone glycosides throughout the entire experimental period. Single light induced limonoid accumulation during exposure periods, but limonoid levels decreased significantly when irradiation was removed. Principal component analysis showed a clear correlation between PMFs and white light, between flavonoid glycosides and red light and UVC, and between limonoids and UVC. These results suggest that the accumulation of flavonoids and limonoids in citrus is regulated by light irradiation. White light, red light and UVC irradiation might be a good potential method for improving the nutrition and flavor quality of post-harvest citrus.

Complementary health food rich in micronutrients

2016 ◽  
Vol 35 (4) ◽  
Author(s):  
Arpitha Gaddam ◽  
Kavita Waghray ◽  
Srinivas Maloo
Keyword(s):  
Dietary Fiber ◽  
Finger Millet ◽  
Control Sample ◽  
Good Health ◽  
The Body ◽  
Health Food ◽  
Hedonic Scale ◽  
Calcium Iron ◽  
Fiber Proteins ◽  
Different Levels

A health food was developed by blending ragi malt (finger millet malt) with oats flour to avail complementary health benefits. Micronutrients are essential for growth and development of children below five years, for proper functioning of every system in the body and are vital for good health. Finger millet is rich source of calcium, iron and dietary fiber, while oats are dense with dietary fiber, proteins, vitamins, minerals and phytochemicals. Ragi malt was blended with oats flour at different levels 10%, 20% and 30% respectively. The incorporated products were subjected to sensory analysis by a panel of 30 members using 5 point Hedonic scale. Formula 1 (10% oats flour formulation) was rated superior to control sample. The superior sample was analyzed and results were compared with FSSAI standards for Malt Based Foods. Shelf life of the product was about 90 days under ambient environment when packed and stored in metallized polyester

Biological Properties of Nanocrystalline Silicon Particles for Biomedical Applications

2006 ◽  
Vol 958 ◽  
Author(s):  
Keisuke Sato ◽  
Satoshi Yanagisawa ◽  
Akio Funakubo ◽  
Yasuhiro Fukui ◽  
Kenji Hirakuri ◽  
...  
Keyword(s):  
Small Intestine ◽  
Biomedical Applications ◽  
Uv Light ◽  
Red Light ◽  
Nanocrystalline Silicon ◽  
Biological Properties ◽  
Light Irradiation ◽  
Uv Light Irradiation ◽  
Luminescence Peak ◽  
Red Luminescence

ABSTRACTWe have studied the biological properties of nanocrystalline silicon (nc-Si) particles after injection at various places in a mouse. The nc-Si particles with a size of 2.5 nm and a concentration of 1.3 mg/ml were dispersed in a normal saline solution (NSS). The NSS dispersible nc-Si particles were safely injected into the mouse. When the nc-Si particles in the NSS were directly injected into the subcutaneous vein and the coronary artery of the heart by syringe, the condition of bloodstream at each place was confirmed by the red luminescence (peak wavelength at 720 nm) from the nc-Si particles under the ultraviolet (UV) light-irradiation. Moreover, the nc-Si particles in the NSS, which were injected into the vein in the sole, smoothly flowed to the small intestine, and the smooth fluidity of nc-Si particles was also observed for the condition of the peristalsis of the small intestine. The nc-Si particles in the small intestine emitted red light during peristalsis under the UV light-irradiation. The red luminescence at each place was very bright and could be clearly seen with the naked eye. These phenomenons were achieved by the utilization of the harmless material, the formation of nc-Si particles with the single-order-size and the realization of the stable surface modification to the nc-Si particles.

Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga

2012 ◽  
Vol 162 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Packo P. Lamers ◽  
Marcel Janssen ◽  
Ric C.H. De Vos ◽  
Raoul J. Bino ◽  
René H. Wijffels
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Dunaliella Salina ◽  
Acid Metabolism ◽  
Green Microalga
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