Persistent Luminescence Cr3+ Doped Spinels and Use as Biomarker for In Vivo Imaging

2014 ◽  
Vol 90 ◽  
pp. 157-165
Author(s):  
Suchinder K. Sharma ◽  
D. Gourier ◽  
B. Viana ◽  
T. Maldiney ◽  
E. Teston ◽  
...  
Keyword(s):  
Uv Light ◽  
Red Light ◽  
Band Gap Energy ◽  
Transparency Window ◽  
Persistent Luminescence ◽  
High Brightness ◽  
Mouse Imaging ◽  
Classical Quantum ◽  
Living Tissues

ZnGa2O4(ZGO) is a normal spinel. When doped with Cr3+ions, ZGO:Cr becomes a high brightness persistent luminescence material with an emission spectrum perfectly matching the transparency window of living tissues. It allowsin vivomouse imaging with a better signal to background ratio than classical quantum dots. The most interesting characteristic of ZGO:Cr lies in the fact that its LLP can be excited with red light, well below its band gap energy and in the transparency window of living tissues. A mechanism based on the trapping of carriers localized around a special type of Cr3+ions namely CrN2can explain this singularity. The antisite defects of the structure are the main responsible traps in the persistent luminescence mechanism. When located around Cr3+ions, they allow, via Cr3+absorption, the storage of not only UV light but also all visible light from the excitation source.

scholarly journals Persistent luminescence materials for deep photodynamic therapy

Nanophotonics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2999-3029 ◽  
Author(s):  
Aurélie Bessière ◽  
Jean-Olivier Durand ◽  
Camille Noûs
Keyword(s):  
Photodynamic Therapy ◽  
Uv Light ◽  
Small Animal Imaging ◽  
Small Animal ◽  
Charge Trapping ◽  
Full Potential ◽  
Persistent Luminescence ◽  
Living Tissues

Abstract Persistent luminescence (PerL) materials continue emitting light long after their excitation has stopped. Prepared in the form of nanoparticles they revealed their full potential as bio-nanoprobes for in vivo small animal imaging in the last 15 years. PerL materials enable to overcome the limitation of weak light penetration in living tissues. As such, they constitute remarkable light mediators to implement photodynamic therapy (PDT) in deep-seated tissues. This article reviews the recent achievements in PerL-mediated PDT in vitro as well as in small animal cancer models in vivo. PerL-mediated PDT is realized through the smart choice of a tandem of a PerL material and a photosensitizer (PS). The physical association of the PerL material and the PS as well as their targeting ability is debated. Implants or mesoporous nanoparticles emerge as particularly valuable cargos that further permit multimodality in imaging or therapy. The diversity of charge-trapping mechanisms in a few PerL materials enables a large versatility in the excitation protocols. Although the PerL agent can be pre-excited by UV light before its introduction into the animal, it also induces effective PDT after simple infrared or visible LED illumination across tissues as well as after a mild X-ray irradiation.

scholarly journals Persistent luminescence nanoparticles for cancer theranostics application

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Nian Liu ◽  
Xiao Chen ◽  
Xia Sun ◽  
Xiaolian Sun ◽  
Junpeng Shi
Keyword(s):  
Uv Light ◽  
Combined Therapy ◽  
Tumor Therapy ◽  
High Sensitivity ◽  
Persistent Luminescence ◽  
Therapeutic Drugs ◽  
High Penetration ◽  
Recent Developments ◽  
Cancer Theranostics

AbstractPersistent luminescence nanoparticles (PLNPs) are unique optical materials that emit afterglow luminescence after ceasing excitation. They exhibit unexpected advantages for in vivo optical imaging of tumors, such as autofluorescence-free, high sensitivity, high penetration depth, and multiple excitation sources (UV light, LED, NIR laser, X-ray, and radiopharmaceuticals). Besides, by incorporating other functional molecules, such as photosensitizers, photothermal agents, or therapeutic drugs, PLNPs are also widely used in persistent luminescence (PersL) imaging-guided tumor therapy. In this review, we first summarize the recent developments in the synthesis and surface functionalization of PLNPs, as well as their toxicity studies. We then discuss the in vivo PersL imaging and multimodal imaging from different excitation sources. Furthermore, we highlight PLNPs-based cancer theranostics applications, such as fluorescence-guided surgery, photothermal therapy, photodynamic therapy, drug/gene delivery and combined therapy. Finally, future prospects and challenges of PLNPs in the research of translational medicine are also discussed.

Improvement of Near-Infrared Persistent Luminescence in Lithium Substituted MgGa2O4:Cr3+ Nanocrystals

2020 ◽  
Vol 15 (5) ◽  
pp. 630-636
Author(s):  
Yue Xu ◽  
Cuiwei Xue ◽  
Shengnan Xue ◽  
Qi Zhu
Keyword(s):  
Oxygen Vacancies ◽  
Near Infrared ◽  
Uv Light ◽  
Sol Gel ◽  
Persistent Luminescence ◽  
Tetrahedral Sites ◽  
Uv Light Irradiation ◽  
Nir Emission ◽  
Gel Processing

Near infrared (NIR) persistent phosphors can exhibit NIR emission for a long period of time after the remove of irradiation, and they have attracted much attention since the demonstration that NIR persistent luminescent nano-sized particles can be used for in vivo imaging. Here, a new NIR persistent luminescence material of lithium substituted MgGa2O4:Cr3+ (termed as MGO:Cr3+, Li+) has been successfully synthesized by sol–gel processing. The MGO:Cr3+, Li+ mainly consists of ∼40–100 nm nanocrystals, which are of single crystalline with excellent crystallinity. Li+ ions occupying the tetrahedral sites (Mg site) does not significantly affect the crystal structure, but it induces a broader band gap. In the processing of substitution for the Mg2+ site by Li+, oxygen vacancies appear that can efficiently store the excited electrons by UV light irradiation and contribute to the improved persistent luminescence. Mg vacancy arising from a higher calcination temperature tends to store visible light, which also contributes to the enhanced afterglow intensity.

Toxicity Effect of Cancer Cell Labeled with Visible Luminescent Nanocrystalline Silicon Particles and Visualization Observationin Vivo

2008 ◽  
Vol 1145 ◽  
Author(s):  
Keisuke Sato ◽  
Masaki Hiruoka ◽  
Kohki Fujioka ◽  
Naoki Fukata ◽  
Kenji Hirakuri ◽  
...  
Keyword(s):  
Hela Cells ◽  
Uv Light ◽  
Light Exposure ◽  
Red Light ◽  
Nanocrystalline Silicon ◽  
Cellular Membrane ◽  
Cervical Carcinoma Cell Line ◽  
Cell Line Hela ◽  
Before And After

AbstractCytotoxicity of human cervical carcinoma cell line (HeLa cells) labeled with the nanocrystalline silicon (nc-Si) particles before and after ultraviolet (UV) light exposure has studied on the viability and cellular membrane damages. The viability and cellular membrane damages of HeLa cells changed at high particle concentration of 1.12 mg/ml. The viability of HeLa cells labeled with the UV-exposed nc-Si particles was higher than that of unexposed nc-Si particles. However, the variation of cellular membrane damages was almost same for the nc-Si particles before and after UV exposure. These results substantiated the low toxicity of nc-Si particles. Moreover, the HeLa cells labeled with the nc-Si particles exhibited green fluorescence. On the other hand,in vivotest of nc-Si particles estimated by the visualization observation of the circulation from the lymphatic vessel to the lymph node of a mouse. The transfer pathway of nc-Si particles could be clearly monitored by the strong emission of red light.

scholarly journals Stability and ocular biodistribution of topically administered PLGA nanoparticles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sean Swetledge ◽  
Renee Carter ◽  
Rhett Stout ◽  
Carlos E. Astete ◽  
Jangwook P. Jung ◽  
...  
Keyword(s):  
Uv Light ◽  
Light Exposure ◽  
Polymeric Nanoparticles ◽  
Plga Nanoparticles ◽  
Eye Disease ◽  
Control Group ◽  
Eye Tissues ◽  
Nanoparticle Morphology ◽  
The Stability

AbstractPolymeric nanoparticles have been investigated as potential delivery systems for therapeutic compounds to address many ailments including eye disease. The stability and spatiotemporal distribution of polymeric nanoparticles in the eye are important regarding the practical applicability and efficacy of the delivery system in treating eye disease. We selected poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with lutein, a carotenoid antioxidant associated with eye health, as our model ophthalmic nanodelivery system and evaluated its stability when suspended in various conditions involving temperature and light exposure. We also assessed the ocular biodistribution of the fluorescently labeled nanoparticle vehicle when administered topically. Lutein-loaded nanoparticles were stable in suspension when stored at 4 °C with only 26% lutein release and no significant lutein decay or changes in nanoparticle morphology. When stored at 25 °C and 37 °C, these NPs showed signs of bulk degradation, had significant lutein decay compared to 4 °C, and released over 40% lutein after 5 weeks in suspension. Lutein-loaded nanoparticles were also more resistant to photodegradation compared to free lutein when exposed to ultraviolet (UV) light, decaying approximately 5 times slower. When applied topically in vivo, Cy5-labled nanoparticles showed high uptake in exterior eye tissues including the cornea, episcleral tissue, and sclera. The choroid was the only inner eye tissue that was significantly higher than the control group. Decreased fluorescence in all exterior eye tissues and the choroid at 1 h compared to 30 min indicated rapid elimination of nanoparticles from the eye.

scholarly journals Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels

2021 ◽  
Vol 12 (2) ◽  
pp. 30
Author(s):  
Shabir Hassan ◽  
Berivan Cecen ◽  
Ramon Peña-Garcia ◽  
Fernanda Roberta Marciano ◽  
Amir K. Miri ◽  
...  
Keyword(s):  
Prolonged Release ◽  
Therapeutic Approaches ◽  
Encapsulated Cells ◽  
Hypoxic Conditions ◽  
Skeletal Myoblasts ◽  
Artificial Tissue ◽  
Delivery Platform ◽  
Living Tissues

Different strategies have been employed to provide adequate nutrients for engineered living tissues. These have mainly revolved around providing oxygen to alleviate the effects of chronic hypoxia or anoxia that result in necrosis or weak neovascularization, leading to failure of artificial tissue implants and hence poor clinical outcome. While different biomaterials have been used as oxygen generators for in vitro as well as in vivo applications, certain problems have hampered their wide application. Among these are the generation and the rate at which oxygen is produced together with the production of the reaction intermediates in the form of reactive oxygen species (ROS). Both these factors can be detrimental for cell survival and can severely affect the outcome of such studies. Here we present calcium peroxide (CPO) encapsulated in polycaprolactone as oxygen releasing microparticles (OMPs). While CPO releases oxygen upon hydrolysis, PCL encapsulation ensures that hydrolysis takes place slowly, thereby sustaining prolonged release of oxygen without the stress the bulk release can endow on the encapsulated cells. We used gelatin methacryloyl (GelMA) hydrogels containing these OMPs to stimulate survival and proliferation of encapsulated skeletal myoblasts and optimized the OMP concentration for sustained oxygen delivery over more than a week. The oxygen releasing and delivery platform described in this study opens up opportunities for cell-based therapeutic approaches to treat diseases resulting from ischemic conditions and enhance survival of implants under severe hypoxic conditions for successful clinical translation.

Cr3+/Y3+ co-doped persistent luminescence nanoparticles with biological window activation for in vivo repeatable imaging

2021 ◽  
Author(s):  
Huimin Jiang ◽  
Lin Liu ◽  
Kexin Yu ◽  
Xianggui Yin ◽  
Shenghui Zheng ◽  
...  
Keyword(s):  
Persistent Luminescence ◽  
Co Doped

scholarly journals Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 643
Author(s):  
Martina De Landro ◽  
Ignacio Espíritu García-Molina ◽  
Manuel Barberio ◽  
Eric Felli ◽  
Vincent Agnus ◽  
...  
Keyword(s):  
Thermal Damage ◽  
Thermal State ◽  
Preliminary Investigation ◽  
Spectral Band ◽  
Spectral Variation ◽  
Ablation Therapy ◽  
Clinical Endpoints ◽  
Breathing Motion ◽  
Living Tissues

This work presents the potential of hyperspectral imaging (HSI) to monitor the thermal outcome of laser ablation therapy used for minimally invasive tumor removal. Our main goal is the establishment of indicators of the thermal damage of living tissues, which can be used to assess the effect of the procedure. These indicators rely on the spectral variation of temperature-dependent tissue chromophores, i.e., oxyhemoglobin, deoxyhemoglobin, methemoglobin, and water. Laser treatment was performed at specific temperature thresholds (from 60 to 110 °C) on in-vivo animal liver and was assessed with a hyperspectral camera (500–995 nm) during and after the treatment. The indicators were extracted from the hyperspectral images after the following processing steps: the breathing motion compensation and the spectral and spatial filtering, the selection of spectral bands corresponding to specific tissue chromophores, and the analysis of the areas under the curves for each spectral band. Results show that properly combining spectral information related to deoxyhemoglobin, methemoglobin, lipids, and water allows for the segmenting of different zones of the laser-induced thermal damage. This preliminary investigation provides indicators for describing the thermal state of the liver, which can be employed in the future as clinical endpoints of the procedure outcome.

scholarly journals Resistance and Adaptation of Bacteria to Non-Antibiotic Antibacterial Agents: Physical Stressors, Nanoparticles, and Bacteriophages

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 435
Author(s):  
Sada Raza ◽  
Kinga Matuła ◽  
Sylwia Karoń ◽  
Jan Paczesny
Keyword(s):  
Antimicrobial Resistance ◽  
Antibacterial Agents ◽  
Uv Light ◽  
Resistance Mechanisms ◽  
Resistant Bacteria ◽  
Physical Factors ◽  
Defense Systems ◽  
Drug Resistant Bacteria

Antimicrobial resistance is a significant threat to human health worldwide, forcing scientists to explore non-traditional antibacterial agents to support rapid interventions and combat the emergence and spread of drug resistant bacteria. Many new antibiotic-free approaches are being developed while the old ones are being revised, resulting in creating unique solutions that arise at the interface of physics, nanotechnology, and microbiology. Specifically, physical factors (e.g., pressure, temperature, UV light) are increasingly used for industrial sterilization. Nanoparticles (unmodified or in combination with toxic compounds) are also applied to circumvent in vivo drug resistance mechanisms in bacteria. Recently, bacteriophage-based treatments are also gaining momentum due to their high bactericidal activity and specificity. Although the number of novel approaches for tackling the antimicrobial resistance crisis is snowballing, it is still unclear if any proposed solutions would provide a long-term remedy. This review aims to provide a detailed overview of how bacteria acquire resistance against these non-antibiotic factors. We also discuss innate bacterial defense systems and how bacteriophages have evolved to tackle them.

Near-infrared persistent luminescence hollow mesoporous nanospheres for drug delivery and in vivo renewable imaging

2016 ◽  
Vol 4 (48) ◽  
pp. 7845-7851 ◽  
Author(s):  
Junpeng Shi ◽  
Meng Sun ◽  
Xia Sun ◽  
Hongwu Zhang
Keyword(s):  
Drug Delivery ◽  
Near Infrared ◽  
High Sensitivity ◽  
Drug Carriers ◽  
Template Method ◽  
Persistent Luminescence ◽  
Deep Tissue ◽  
Large Capacity

Near-infrared persistent luminescence hollow mesoporous nanospheres have been synthesized via a template method. These nanospheres can be used as large capacity drug carriers and realize super long-term and high sensitivity tracking of drug delivery in deep tissue.

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