scholarly journals A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation

Marine Drugs ◽  
2022 ◽  
Vol 20 (1) ◽  
pp. 48
Author(s):  
Jian Li ◽  
Jun-Hui Cheng ◽  
Zhao-Jie Teng ◽  
Xia Zhang ◽  
Xiu-Lan Chen ◽  
...  
Keyword(s):  
Potential Application ◽  
Domain Architecture ◽  
Bone Collagen ◽  
Bovine Bone ◽  
Hydrolysis Time ◽  
Collagen Peptide ◽  
Enzyme Substrate ◽  
Hydrolysis Temperature ◽  
Merops Database ◽  
Hydrolysis Conditions

Although the S8 family in the MEROPS database contains many peptidases, only a few S8 peptidases have been applied in the preparation of bioactive oligopeptides. Bovine bone collagen is a good source for preparing collagen oligopeptides, but has been so far rarely applied in collagen peptide preparation. Here, we characterized a novel S8 gelatinase, Aa2_1884, from marine bacterium Flocculibacter collagenilyticus SM1988T, and evaluated its potential application in the preparation of collagen oligopeptides from bovine bone collagen. Aa2_1884 is a multimodular S8 peptidase with a distinct domain architecture from other reported peptidases. The recombinant Aa2_1884 over-expressed in Escherichia coli showed high activity toward gelatin and denatured collagens, but no activity toward natural collagens, indicating that Aa2_1884 is a gelatinase. To evaluate the potential of Aa2_1884 in the preparation of collagen oligopeptides from bovine bone collagen, three enzymatic hydrolysis parameters, hydrolysis temperature, hydrolysis time and enzyme-substrate ratio (E/S), were optimized by single factor experiments, and the optimal hydrolysis conditions were determined to be reaction at 60 ℃ for 3 h with an E/S of 400 U/g. Under these conditions, the hydrolysis efficiency of bovine bone collagen by Aa2_1884 reached 95.3%. The resultant hydrolysate contained 97.8% peptides, in which peptides with a molecular weight lower than 1000 Da and 500 Da accounted for 55.1% and 39.5%, respectively, indicating that the hydrolysate was rich in oligopeptides. These results indicate that Aa2_1884 likely has a promising potential application in the preparation of collagen oligopeptide-rich hydrolysate from bovine bone collagen, which may provide a feasible way for the high-value utilization of bovine bone collagen.

Preparation of Microcrystalline Cellulose from Cotton Linter Ethanol Pulp

2011 ◽  
Vol 295-297 ◽  
pp. 653-658
Author(s):  
Shan Shan Liu ◽  
Gui Gan Fang ◽  
Yong Jun Deng ◽  
Qiang Wang
Keyword(s):  
Particle Size ◽  
Factorial Design ◽  
Microcrystalline Cellulose ◽  
Experimental Results ◽  
Cotton Linter ◽  
Hydrolysis Time ◽  
Liquor Ratio ◽  
Hydrolysis Temperature ◽  
Hydrolysis Conditions

The hydrolysis trials of produce microcrystalline cellulose from cotton linter ethnaol pulp by hydrochloric were investigated. Based on 23 factorial design, fifteen operations were performed by varying S/L, T and t (S/L: solid to liquor ratio, T: hydrolysis temperature, t: hydrolysis time). The charecteristic of product was analyzed by X-RD, SEM and laser granularity distributing apparatus, and compared with commercial MCC. The experimental results indicated that MCC yield and particle size were influenced significantly by those factors. The optimal hydrolysis conditions was S/L=1:7, T=60°C and t=40min, which can resulted MCC yield of 75.3% and size of 21.21µm. Crystallinity of MCC is 63.96% and displayed as rod-shape.

Study on Extractiing of Heme from Tilapias Blood by Enzymatic Method

2014 ◽  
Vol 941-944 ◽  
pp. 2641-2645
Author(s):  
Wan Jun Zhou ◽  
Jian Wei Cen ◽  
Qing Rong Hu ◽  
Shu Xian Hao ◽  
Lai Hao Li ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Acid Precipitation ◽  
Enzymatic Method ◽  
Optimum Temperature ◽  
Hydrolysis Time ◽  
Enzyme Substrate ◽  
Orthogonal Experiments ◽  
Optimum Ph ◽  
Theoretical Evidence ◽  
Hydrolysis Conditions

To make sure the abundance of Tilapias blood resources in China can be utilized more scientificly and effectively, the extraction conditions of heme from Tilapias blood by enzymatic hydrolysis were studied in this paper. The optimal hydrolysis conditions of protamex have been fixed by the single factor and the orthogonal experiments: The optimum temperature of 40°C, optimum pH of 8.0, substrate concentration (S) was 6%, enzyme/substrate (E/S) was 8000U/g, hydrolysis time (T) was 2 hours. Followed by the heat treatment, acid precipitation and vacuum freeze drying could get the heme at last, which yield was 80.9%, purity was 28.2%. This technology is simple and practical, low production cost and suitable for industrialized production, which is not only reduce environmental pollution, but also increase the economic efficiency of enterprises effectively. This research could provide theoretical evidence for making the best use of Tilapias blood resources.

scholarly journals Effect of Hydrolysis on Tannin and Carotenoid Contents, and Antioxidant Activity of Pouteria campechiana

2021 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Hien Xuan Tran ◽  
Huong Lien Huynh ◽  
Thanh Trung Nguyen
Keyword(s):  
Antioxidant Activity ◽  
Food Industry ◽  
Enzyme Concentration ◽  
Scientific Data ◽  
Hydrolysis Time ◽  
Ic50 Value ◽  
Hydrolysis Temperature ◽  
Medicinal Properties ◽  
Highly Correlated ◽  
Hydrolysis Conditions

The medicinal properties of Pouteria campechiana fruit in Vietnam currently have not been studied much. This study was conducted to evaluate hydrolysis's effect on the carotenoid, tannin, and antioxidant activity through the correlation between IC50 and TPC values of Pouteria campechiana extract. This study examined hydrolysis conditions, such as enzyme type, enzyme concentration, temperature, and hydrolysis time. Experimental results showed that at pectinase enzyme concentration of 0.6 wt%, cellulase enzyme concentration of 0.6 wt%, at hydrolysis temperature of 600C, and 65 minutes for hydrolysis, the study found carotenoid of 115.14±4.14 (µg/g) and tannin of 45.88±2.37 (mgTAE/g)in the extract. IC50 value (7.82±0.21 mg/mL) and TPC content were highly correlated (R2=0.98). This study's results contributed to the provision of valuable scientific data on Pouteria campechiana fruit, especially for the food industry

scholarly journals Production of fish seasoning powder from snakehead meat (Channa striata) by applying Alcalase and Flavourzyme enzyme mixture

2020 ◽  
Vol 19 (02) ◽  
pp. 43-49
Keyword(s):  
Moisture Content ◽  
Amino Acid Content ◽  
Hydrolysis Time ◽  
Ammonia Content ◽  
Fish Meat ◽  
Enzyme Mixture ◽  
Sensory Score ◽  
Hydrolysis Temperature ◽  
Channa Striata ◽  
Hydrolysis Conditions

Fish hydrolysate was produced from snakehead meat by applying an enzyme mixture consisting of Alcalase and Flavourzyme with a ratio of 1:3; hydrolysis temperature 55oC; pH 6.5 - 6.9. The results showed that the ratio of enzyme mixture to fish meat of 0.2% and hydrolysis time of 26 h were the optimal hydrolysis conditions. Fish hydrolysate had highest peptide content (26.4 g/L) and nitrogen amino acid content (10.6 g/L), and lowest nitrogen ammonia content (0.257 g/L). Sensory score, protein content, recovery yield and moisture content of fish seasoning powder were 18.9; 17.1%; 42.5% and 4.88%, respectively by mixing radish solution and fish hydrolysate at the ratio of radish solution to fish hydrolysate of 25%:40% and drying at 60oC for 72 h. The product still remained good sensory quality. The moisture content and total aerobic bacteria of product were at acceptable level after 4 weeks stored at ambient temperature.

The Optimal Conditions on Excess Sludge Hydrolysis and Reduction Using Multi-Enzyme

2014 ◽  
Vol 955-959 ◽  
pp. 2889-2892
Author(s):  
Liang Guo ◽  
Ming Min Lu ◽  
Yan Zong
Keyword(s):  
Treatment Process ◽  
Environmental Problem ◽  
Sewage Treatment ◽  
Excess Sludge ◽  
Hydrolysis Time ◽  
Sludge Digestion ◽  
Treatment Conditions ◽  
Hydrolysis Temperature ◽  
Sludge Hydrolysis ◽  
Hydrolysis Conditions

A large quantity of excess sludge is produced during the sewage treatment process, which will cause serious environmental problem without being treated properly. In this paper, a new multi-enzyme was added in the excess sludge to enhance the sludge hydrolysis and reduction, and the treatment conditions of hydrolysis time, dosage, hydrolysis temperature with multi-enzymes was optimized in this study. The multi-enzymes could promote the solubilization of suspended sludge solids and the degradation of macromolecular organic matter. The effect of excess sludge digestion was enhanced significantly at the condition of 30 °C, 1.5 mL multi-enzyme after 5 h hydrolysis for per 100 mL excess sludge. And the optimal hydrolysis conditions were determined by detecting the concentration of carbohydrate, soluble protein, SCOD and sludge’s lysis rates, and the effect of sludge reduction was investigatied by the VSS solubility.

scholarly journals Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2844 ◽  
Author(s):  
Xueqin Wang ◽  
Huahua Yu ◽  
Ronge Xing ◽  
Song Liu ◽  
Xiaolin Chen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Enzyme Concentration ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Hydrolysis Temperature ◽  
Hydrolysis Conditions ◽  
Different Response

Oyster (Crassostrea talienwhanensis) protein was hydrolyzed by trypsin to produce peptides with different response values, and response surface methodology (RSM) was applied to optimize the hydrolysis conditions. The highest degree of hydrolysis (DH) of the oyster peptide (OP) was obtained at an enzyme concentration of 1593.2 U/g, a pH of 8.2, a hydrolysis temperature of 40.1 °C, a hydrolysis time of 6.0 h, and a water/material ratio of 8.2. The greatest hydroxyl-radical-scavenging activity of OP was obtained at an enzyme concentration of 1546.3 U/g, a pH of 9.0, a hydrolysis temperature of 50.2 °C, a hydrolysis time of 5.1 h, and a water/material ratio of 5.6. The largest branched-chain amino acid (BCAA) content of OP was obtained at an enzyme concentration of 1323.8 U/g, a pH of 8.3, a hydrolysis temperature of 41.7 °C, a hydrolysis time of 6.7 h, and a water/material ratio of 4.8. The three experimental values were significantly in agreement with the predicted values within the 95% confidence interval. Furthermore, ultrafiltration and chromatographic methods were used to purify the OP, and 13 peptides that were rich in Lys, Arg, His, and Thr were identified by LC-MS/MS. The results of this study offer different optimum hydrolysis conditions to produce target peptides from oyster protein by using RSM, and this study provide a theoretical basis for the high-value utilization of oyster protein.

scholarly journals Potential of Thermolysin-like Protease A69 in Preparation of Bovine Collagen Peptides with Moisture-Retention Ability and Antioxidative Activity

Marine Drugs ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. 676
Author(s):  
Jun-Hui Cheng ◽  
Xiao-Yu Zhang ◽  
Zhen Wang ◽  
Xia Zhang ◽  
Shi-Cheng Liu ◽  
...  
Keyword(s):  
Antioxidative Activity ◽  
Bone Collagen ◽  
Bovine Bone ◽  
Moisture Retention ◽  
Collagen Peptides ◽  
Collagen Peptide ◽  
Bovine Collagen ◽  
Pharmaceutical Industries ◽  
Scavenging Ratio ◽  
Set Up

Bovine bone is rich in collagen and is a good material for collagen peptide preparation. Although thermolysin-like proteases (TLPs) have been applied in different fields, the potential of TLPs in preparing bioactive collagen peptides has rarely been evaluated. Here, we characterized a thermophilic TLP, A69, from a hydrothermal bacterium Anoxybacillus caldiproteolyticus 1A02591, and evaluated its potential in preparing bioactive collagen peptides. A69 showed the highest activity at 60 °C and pH 7.0. We optimized the conditions for bovine bone collagen hydrolysis and set up a process with high hydrolysis efficiency (99.4%) to prepare bovine bone collagen peptides, in which bovine bone collagen was hydrolyzed at 60 °C for 2 h with an enzyme–substrate ratio of 25 U/g. The hydrolysate contained 96.5% peptides that have a broad molecular weight distribution below 10000 Da. The hydrolysate showed good moisture-retention ability and a high hydroxyl radical (•OH) scavenging ratio of 73.2%, suggesting that the prepared collagen peptides have good antioxidative activity. Altogether, these results indicate that the thermophilic TLP A69 has promising potential in the preparation of bioactive collagen peptides, which may have potentials in cosmetics, food and pharmaceutical industries. This study lays a foundation for the high-valued utilization of bovine bone collagen.

Identification and antioxidant activity of bovine bone collagen-derived novel peptides prepared by recombinant collagenase from Bacillus cereus

2021 ◽  
Vol 349 ◽  
pp. 129143
Author(s):  
Yihang Song ◽  
Yousi Fu ◽  
Shiyang Huang ◽  
Langxing Liao ◽  
Qian Wu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bacillus Cereus ◽  
Bone Collagen ◽  
Bovine Bone

scholarly journals The Effect of Electronic Medical Record Adaptation on Reported Medication Errors in Peripartum Care Areas

2017 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Sulfuric Acid ◽  
Acid Concentration ◽  
Cellulose Hydrolysis ◽  
Colloidal Dispersions ◽  
Degree Of Polymerization ◽  
Degree Of Crystallinity ◽  
Minimum Level ◽  
Hydrolysis Temperature ◽  
Hydrolysis Conditions ◽  
Negative Zeta Potential

Relationship between concentration of sulfuric acid (C) and temperature (T) required for the cellulose hydrolysis to prepare nanocrystalline cellulose particles (NCP has been studied in this paper. The experiments showed that there is a linear C and T superposition exists, namely: C= Co - n T. The higher the acid concentration, the lower the hydrolysis temperature should be used, and vice versa. The minimum level of acid concentration that can be used for production of CNP is 40 wt. % at optimal temperature of 80o C. If temperature or acid concentration is lower, there is a tendency to form of microparticles. On the other hand, at higher hydrolysis temperatures, carbonized CNP with decreased yield are obtained. The rod-like crystalline nanoparticles of cellulose produced at optimal hydrolysis conditions (C=40 wt. %, T=80o C) have average sizes of 150 x 15 nm, degree of crystallinity of 75- 77% and degree of polymerization of 130-150. The nanoparticles form stable colloidal dispersions in water due to Brownian motion and negative Zeta potential imparting to these particles the mutual electrostatic repulsion. Estimated calculations have also shown that decrease in the concentration of sulfuric acid from 60 to 40 wt. % at production of CNP is economically advantageous despite increase in hydrolysis temperature from 45 to 80o C.

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