scholarly journals Production of fish seasoning powder from snakehead meat (Channa striata) by applying Alcalase and Flavourzyme enzyme mixture

2020 ◽  
Vol 19 (02) ◽  
pp. 43-49
Keyword(s):  
Moisture Content ◽  
Amino Acid Content ◽  
Hydrolysis Time ◽  
Ammonia Content ◽  
Fish Meat ◽  
Enzyme Mixture ◽  
Sensory Score ◽  
Hydrolysis Temperature ◽  
Channa Striata ◽  
Hydrolysis Conditions

Fish hydrolysate was produced from snakehead meat by applying an enzyme mixture consisting of Alcalase and Flavourzyme with a ratio of 1:3; hydrolysis temperature 55oC; pH 6.5 - 6.9. The results showed that the ratio of enzyme mixture to fish meat of 0.2% and hydrolysis time of 26 h were the optimal hydrolysis conditions. Fish hydrolysate had highest peptide content (26.4 g/L) and nitrogen amino acid content (10.6 g/L), and lowest nitrogen ammonia content (0.257 g/L). Sensory score, protein content, recovery yield and moisture content of fish seasoning powder were 18.9; 17.1%; 42.5% and 4.88%, respectively by mixing radish solution and fish hydrolysate at the ratio of radish solution to fish hydrolysate of 25%:40% and drying at 60oC for 72 h. The product still remained good sensory quality. The moisture content and total aerobic bacteria of product were at acceptable level after 4 weeks stored at ambient temperature.

scholarly journals Ekstraksi Gelatin Tulang Ikan Gabus (Channa striata) Dengan Variasi Asam Yang Berbeda Pada Proses Demineralisasi

2020 ◽  
Vol 25 (3) ◽  
pp. 201
Author(s):  
Fitra Mulia Jaya ◽  
Neny Rochyani
Keyword(s):  
Citric Acid ◽  
Moisture Content ◽  
Fish Bone ◽  
Capital City ◽  
Raw Material ◽  
Weak Acids ◽  
Fish Meat ◽  
Randomized Design ◽  
Channa Striata ◽  
Completely Randomized Design

Palembang is the capital city of South Sumatra Province which has special foods which main raw material is fish. Many pempek entrepreneurs use cork fish meat for pempek raw material resulting in abundance of cork fish bone waste, therefore researchers utilize cork fish bone waste as an alternative into raw material in making gelatin. In this study, researchers used several weak acids derived from natural ingredients such as lime extract and starfruit extract and as a comparison researchers used synthetic weak acids namely citric acid in the market. The purpose of this research used several types of acids in the demineralization process to produce better gelatin production. This study used a Completely Randomized Design, with several acid treatments with and consisting of 3 (three) treatment levels, each treatment was repeated 3 times. The treatment in this study was a demineralization process with the addition of citric acid was (A1), lime extract was (A2), and starfruit extract was (A3). The parameters examined in this study include: Gelatin yield, Gelatin acidity (pH), Moisture and Sensory content (color and aroma). Data from proximate observations were analyzed by analysis of variance (ANOVA) and further tested based on the coefficient of diversity. From the results of this study, it was found that the best treatment of using variations in acid was gelatin demineralized using citric acid which produced a yield was 6%, a moisture content was 9.21%, pH 6, the panelist's favorite value of color was 4.3 and aroma 3, 8.

Preparation of Microcrystalline Cellulose from Cotton Linter Ethanol Pulp

2011 ◽  
Vol 295-297 ◽  
pp. 653-658
Author(s):  
Shan Shan Liu ◽  
Gui Gan Fang ◽  
Yong Jun Deng ◽  
Qiang Wang
Keyword(s):  
Particle Size ◽  
Factorial Design ◽  
Microcrystalline Cellulose ◽  
Experimental Results ◽  
Cotton Linter ◽  
Hydrolysis Time ◽  
Liquor Ratio ◽  
Hydrolysis Temperature ◽  
Hydrolysis Conditions

The hydrolysis trials of produce microcrystalline cellulose from cotton linter ethnaol pulp by hydrochloric were investigated. Based on 23 factorial design, fifteen operations were performed by varying S/L, T and t (S/L: solid to liquor ratio, T: hydrolysis temperature, t: hydrolysis time). The charecteristic of product was analyzed by X-RD, SEM and laser granularity distributing apparatus, and compared with commercial MCC. The experimental results indicated that MCC yield and particle size were influenced significantly by those factors. The optimal hydrolysis conditions was S/L=1:7, T=60°C and t=40min, which can resulted MCC yield of 75.3% and size of 21.21µm. Crystallinity of MCC is 63.96% and displayed as rod-shape.

scholarly journals Optimization of Antioxidant Hydrolysate Produced from Tibetan Egg White with Papain and Its Application in Yak Milk Yogurt

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 109
Author(s):  
Fumin Chi ◽  
Ting Liu ◽  
Liu Liu ◽  
Zhankun Tan ◽  
Xuedong Gu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Content ◽  
Egg White ◽  
Scavenging Activity ◽  
Hydrolysis Time ◽  
Food Ingredient ◽  
Yak Milk ◽  
Sensory Score ◽  
Amino Acid Profiles ◽  
Enzyme Dosage

The objective of the present study was to produce antioxidant hydrolysate from Tibetan egg white protein hydrolyzed with papain, and to investigate the effect of added papain egg white hydrolysate (PEWH) on the quality characteristics and amino acid profiles of yak milk yogurt. A response surface methodology (RSM) was utilized to analyze the effects of hydrolysis time (X1), the ratio of enzymes to substrates, and enzyme dosage (X3) on the superoxide anion radical (O2−) scavenging activity of hydrolysates. The predicted maximum value of O2− scavenging activity (89.06%) was obtained an X1 of 2.51 h, X2 of 4.13%, and X3 of 4500 U/g of substrate, almost approaching the experimental value (88.05 ± 1.2%). Furthermore, it was found that the addition of PEWH to yak milk can enhance acidification, sensory score, the number of lactic acid bacteria (LAB), and the amino acid content in yak milk yogurt. The results suggested that PEWH displayed an exceptional potential to be developed as a functional food ingredient that could be applied during the manufacturing process of yak milk yogurt.

scholarly journals A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation

Marine Drugs ◽  
2022 ◽  
Vol 20 (1) ◽  
pp. 48
Author(s):  
Jian Li ◽  
Jun-Hui Cheng ◽  
Zhao-Jie Teng ◽  
Xia Zhang ◽  
Xiu-Lan Chen ◽  
...  
Keyword(s):  
Potential Application ◽  
Domain Architecture ◽  
Bone Collagen ◽  
Bovine Bone ◽  
Hydrolysis Time ◽  
Collagen Peptide ◽  
Enzyme Substrate ◽  
Hydrolysis Temperature ◽  
Merops Database ◽  
Hydrolysis Conditions

Although the S8 family in the MEROPS database contains many peptidases, only a few S8 peptidases have been applied in the preparation of bioactive oligopeptides. Bovine bone collagen is a good source for preparing collagen oligopeptides, but has been so far rarely applied in collagen peptide preparation. Here, we characterized a novel S8 gelatinase, Aa2_1884, from marine bacterium Flocculibacter collagenilyticus SM1988T, and evaluated its potential application in the preparation of collagen oligopeptides from bovine bone collagen. Aa2_1884 is a multimodular S8 peptidase with a distinct domain architecture from other reported peptidases. The recombinant Aa2_1884 over-expressed in Escherichia coli showed high activity toward gelatin and denatured collagens, but no activity toward natural collagens, indicating that Aa2_1884 is a gelatinase. To evaluate the potential of Aa2_1884 in the preparation of collagen oligopeptides from bovine bone collagen, three enzymatic hydrolysis parameters, hydrolysis temperature, hydrolysis time and enzyme-substrate ratio (E/S), were optimized by single factor experiments, and the optimal hydrolysis conditions were determined to be reaction at 60 ℃ for 3 h with an E/S of 400 U/g. Under these conditions, the hydrolysis efficiency of bovine bone collagen by Aa2_1884 reached 95.3%. The resultant hydrolysate contained 97.8% peptides, in which peptides with a molecular weight lower than 1000 Da and 500 Da accounted for 55.1% and 39.5%, respectively, indicating that the hydrolysate was rich in oligopeptides. These results indicate that Aa2_1884 likely has a promising potential application in the preparation of collagen oligopeptide-rich hydrolysate from bovine bone collagen, which may provide a feasible way for the high-value utilization of bovine bone collagen.

scholarly journals Effect of Hydrolysis on Tannin and Carotenoid Contents, and Antioxidant Activity of Pouteria campechiana

2021 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Hien Xuan Tran ◽  
Huong Lien Huynh ◽  
Thanh Trung Nguyen
Keyword(s):  
Antioxidant Activity ◽  
Food Industry ◽  
Enzyme Concentration ◽  
Scientific Data ◽  
Hydrolysis Time ◽  
Ic50 Value ◽  
Hydrolysis Temperature ◽  
Medicinal Properties ◽  
Highly Correlated ◽  
Hydrolysis Conditions

The medicinal properties of Pouteria campechiana fruit in Vietnam currently have not been studied much. This study was conducted to evaluate hydrolysis's effect on the carotenoid, tannin, and antioxidant activity through the correlation between IC50 and TPC values of Pouteria campechiana extract. This study examined hydrolysis conditions, such as enzyme type, enzyme concentration, temperature, and hydrolysis time. Experimental results showed that at pectinase enzyme concentration of 0.6 wt%, cellulase enzyme concentration of 0.6 wt%, at hydrolysis temperature of 600C, and 65 minutes for hydrolysis, the study found carotenoid of 115.14±4.14 (µg/g) and tannin of 45.88±2.37 (mgTAE/g)in the extract. IC50 value (7.82±0.21 mg/mL) and TPC content were highly correlated (R2=0.98). This study's results contributed to the provision of valuable scientific data on Pouteria campechiana fruit, especially for the food industry

The Optimal Conditions on Excess Sludge Hydrolysis and Reduction Using Multi-Enzyme

2014 ◽  
Vol 955-959 ◽  
pp. 2889-2892
Author(s):  
Liang Guo ◽  
Ming Min Lu ◽  
Yan Zong
Keyword(s):  
Treatment Process ◽  
Environmental Problem ◽  
Sewage Treatment ◽  
Excess Sludge ◽  
Hydrolysis Time ◽  
Sludge Digestion ◽  
Treatment Conditions ◽  
Hydrolysis Temperature ◽  
Sludge Hydrolysis ◽  
Hydrolysis Conditions

A large quantity of excess sludge is produced during the sewage treatment process, which will cause serious environmental problem without being treated properly. In this paper, a new multi-enzyme was added in the excess sludge to enhance the sludge hydrolysis and reduction, and the treatment conditions of hydrolysis time, dosage, hydrolysis temperature with multi-enzymes was optimized in this study. The multi-enzymes could promote the solubilization of suspended sludge solids and the degradation of macromolecular organic matter. The effect of excess sludge digestion was enhanced significantly at the condition of 30 °C, 1.5 mL multi-enzyme after 5 h hydrolysis for per 100 mL excess sludge. And the optimal hydrolysis conditions were determined by detecting the concentration of carbohydrate, soluble protein, SCOD and sludge’s lysis rates, and the effect of sludge reduction was investigatied by the VSS solubility.

scholarly journals Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2844 ◽  
Author(s):  
Xueqin Wang ◽  
Huahua Yu ◽  
Ronge Xing ◽  
Song Liu ◽  
Xiaolin Chen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Enzyme Concentration ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Hydrolysis Temperature ◽  
Hydrolysis Conditions ◽  
Different Response

Oyster (Crassostrea talienwhanensis) protein was hydrolyzed by trypsin to produce peptides with different response values, and response surface methodology (RSM) was applied to optimize the hydrolysis conditions. The highest degree of hydrolysis (DH) of the oyster peptide (OP) was obtained at an enzyme concentration of 1593.2 U/g, a pH of 8.2, a hydrolysis temperature of 40.1 °C, a hydrolysis time of 6.0 h, and a water/material ratio of 8.2. The greatest hydroxyl-radical-scavenging activity of OP was obtained at an enzyme concentration of 1546.3 U/g, a pH of 9.0, a hydrolysis temperature of 50.2 °C, a hydrolysis time of 5.1 h, and a water/material ratio of 5.6. The largest branched-chain amino acid (BCAA) content of OP was obtained at an enzyme concentration of 1323.8 U/g, a pH of 8.3, a hydrolysis temperature of 41.7 °C, a hydrolysis time of 6.7 h, and a water/material ratio of 4.8. The three experimental values were significantly in agreement with the predicted values within the 95% confidence interval. Furthermore, ultrafiltration and chromatographic methods were used to purify the OP, and 13 peptides that were rich in Lys, Arg, His, and Thr were identified by LC-MS/MS. The results of this study offer different optimum hydrolysis conditions to produce target peptides from oyster protein by using RSM, and this study provide a theoretical basis for the high-value utilization of oyster protein.

scholarly journals The Effect of Electronic Medical Record Adaptation on Reported Medication Errors in Peripartum Care Areas

2017 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Sulfuric Acid ◽  
Acid Concentration ◽  
Cellulose Hydrolysis ◽  
Colloidal Dispersions ◽  
Degree Of Polymerization ◽  
Degree Of Crystallinity ◽  
Minimum Level ◽  
Hydrolysis Temperature ◽  
Hydrolysis Conditions ◽  
Negative Zeta Potential

Relationship between concentration of sulfuric acid (C) and temperature (T) required for the cellulose hydrolysis to prepare nanocrystalline cellulose particles (NCP has been studied in this paper. The experiments showed that there is a linear C and T superposition exists, namely: C= Co - n T. The higher the acid concentration, the lower the hydrolysis temperature should be used, and vice versa. The minimum level of acid concentration that can be used for production of CNP is 40 wt. % at optimal temperature of 80o C. If temperature or acid concentration is lower, there is a tendency to form of microparticles. On the other hand, at higher hydrolysis temperatures, carbonized CNP with decreased yield are obtained. The rod-like crystalline nanoparticles of cellulose produced at optimal hydrolysis conditions (C=40 wt. %, T=80o C) have average sizes of 150 x 15 nm, degree of crystallinity of 75- 77% and degree of polymerization of 130-150. The nanoparticles form stable colloidal dispersions in water due to Brownian motion and negative Zeta potential imparting to these particles the mutual electrostatic repulsion. Estimated calculations have also shown that decrease in the concentration of sulfuric acid from 60 to 40 wt. % at production of CNP is economically advantageous despite increase in hydrolysis temperature from 45 to 80o C.

scholarly journals Physical Characteristics of Gelatin from Cork Fish skin in The Waters of Merauke

2020 ◽  
Vol 147 ◽  
pp. 03027
Author(s):  
Diah Lestari Ayudiarti ◽  
Ema Hastarini ◽  
Rini Susilowati
Keyword(s):  
Acetic Acid ◽  
Gel Strength ◽  
Physical Characteristics ◽  
High Protein ◽  
Fish Skin ◽  
Fish Meat ◽  
Channa Striata ◽  
Very High

Cork fish (Channa striata) is a swamp fish that population very abundant in Merauke. This fish meat is rich in albumin and is usually used as an additional supplement for people who have undergone surgery. Cork fish skin also contains very high protein, which one of them is gelatin. Research on physical characteristics of gelatin from cork fish skin in the waters of merauke has been done. Gelatin were made by soaked the cork fish skin into acetic acid 0.5 M at concentration 2, 4 and 6% for12 hours. After soaked, the skins were washed by water until neutralized (pH 6), then it were extracted using aquadest (1:3) at 70oC for 2 hours. The filtrates then were filtered and dried in oven at temperature 40-50oC. The result showed that the best gelatin extraction was using acetic acid 0.5 M in concentration 6% with yield 10.15%, gel strength 169.45 gbloom, viscosity 14.31 cPs, white degree 52.

Study on the BTSPS Silane Film Prepared on the Surface of the 2A12 Aluminum Alloy

2014 ◽  
Vol 936 ◽  
pp. 1071-1076
Author(s):  
Long Jiang Peng ◽  
Shao Hui Chen ◽  
Ying Ning Feng ◽  
Tian Yi Li
Keyword(s):  
Aluminum Alloy ◽  
Volume Fraction ◽  
Orthogonal Experiment ◽  
Influence Factors ◽  
Dip Coating ◽  
Hydrolysis Time ◽  
Electrochemical Test ◽  
Coating Method ◽  
Hydrolysis Temperature ◽  
Dip Coating Method

The protective silane film BTSPS was prepared on the surface of 2A12 aluminum alloys by dip-coating method in this paper. The influence factors such as the volume fraction, hydrolysis time, hydrolysis temperature and dip-coating time of BTSPS were studied through orthogonal experiment. The optimum conditions is: the volume fraction is 5%; hydrolysis time is 180min; Hydrolysis temperature is 20°C; Dipping time is 10 min. The silane film performance was investigated by electrochemical test, sodium chloride solution immersion and scanning electron microscope (SEM). The results show that the corrosion resistance of the 2A12 aluminum alloy coated with BTSPS is improved greatly.

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