Development of a Microfluidic Platform for Trace Lipid Analysis

The inherent trace quantity of primary fatty acid amides found in biological systems presents challenges for analytical analysis and quantitation, requiring a highly sensitive detection system. The use of microfluidics provides a green sample preparation and analysis technique through small-volume fluidic flow through micron-sized channels embedded in a polydimethylsiloxane (PDMS) device. Microfluidics provides the potential of having a micro total analysis system where chromatographic separation, fluorescent tagging reactions, and detection are accomplished with no added sample handling. This study describes the development and the optimization of a microfluidic-laser induced fluorescence (LIF) analysis and detection system that can be used for the detection of ultra-trace levels of fluorescently tagged primary fatty acid amines. A PDMS microfluidic device was designed and fabricated to incorporate droplet-based flow. Droplet microfluidics have enabled on-chip fluorescent tagging reactions to be performed quickly and efficiently, with no additional sample handling. An optimized LIF optical detection system provided fluorescently tagged primary fatty acid amine detection at sub-fmol levels (436 amol). The use of this LIF detection provides unparalleled sensitivity, with detection limits several orders of magnitude lower than currently employed LC-MS techniques, and might be easily adapted for use as a complementary quantification platform for parallel MS-based omics studies.

Download Full-text

Development of a Microfluidic Platform for Trace Lipid Analysis

10.20944/preprints202010.0031.v1 ◽

2020 ◽

Author(s):

Andrew Davic ◽

Michael Cascio

Keyword(s):

Fatty Acid ◽

Lipid Analysis ◽

Detection System ◽

Droplet Microfluidics ◽

Trace Quantity ◽

Sample Handling ◽

Fatty Acid Amides ◽

Total Analysis System ◽

Fluorescent Tagging ◽

Highly Sensitive Detection

The inherent trace quantity of primary fatty acid amides found in biological systems presents challenges for analytical analysis and quantitation, requiring a highly sensitive detection system. The use of microfluidics provides a green sample preparation and analysis technique through small-volume fluidic flow through micron-sized channels embedded in a PDMS device. Microfluidics provides the potential of having a micro total analysis system where chromatographic separation, fluorescent tagging reactions, and detection are accomplished with no added sample handling. This study describes the development and optimization of a microfluidic-laser indued fluorescence (LIF) analysis and detection system that can be used for the detection of ultra-trace levels of fluorescently tagged primary fatty acid amines. A PDMS microfluidic device was designed and fabricated to incorporate droplet-based flow. Droplet microfluidics have enabled on-chip fluorescent tagging reactions to be performed quickly and efficiently, with no additional sample handling. An optimized LIF optical detection system provided fluorescently tagged primary fatty acid amine detection sub-fmol (436 amol) LODs. The use of this LIF detection provides unparalleled sensitivity, with detection limits several orders of magnitude lower than currently employed LC-MS techniques and might be easily adapted for use as a complementary quantification platform for parallel MS-based -omics studies.

Download Full-text

A new method for quantifying the blocking of coated paperboard

TAPPI Journal ◽

10.32964/tj9.5.29 ◽

2010 ◽

Vol 9 (5) ◽

pp. 29-35 ◽

Cited By ~ 2

Author(s):

PAULINE SKILLINGTON ◽

YOLANDE R. SCHOEMAN ◽

VALESKA CLOETE ◽

PATRICE C. HARTMANN

Keyword(s):

Surface Roughness ◽

Fatty Acid ◽

Surface Energy ◽

Film Thickness ◽

External Factors ◽

Additive Concentration ◽

Pressure Surface ◽

Fatty Acid Amides ◽

Coated Surfaces ◽

Definite Period

Blocking is undesired adhesion between two surfaces when subjected to pressure and temperature constraints. Blocking between two coated paperboards in contact with each other may be caused by inter-diffusion, adsorption, or electrostatic forces occurring between the respective coating surfaces. These interactions are influenced by factors such as the temperature, pressure, surface roughness, and surface energy. Blocking potentially can be reduced by adjusting these factors, or by using antiblocking additives such as talc, amorphous silica, fatty acid amides, or polymeric waxes. We developed a method of quantifying blocking using a rheometer. Coated surfaces were put in contact with each other with controlled pressure and temperature for a definite period. We then measured the work necessary to pull the two surfaces apart. This was a reproducible way to accurately quantify blocking. The method was applied to determine the effect external factors have on the blocking tendency of coated paperboards, i.e., antiblocking additive concentration, film thickness, temperature, and humidity.

Download Full-text

Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Molecules ◽

10.3390/molecules26092543 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2543

Author(s):

Ruidong Ni ◽

Suzeeta Bhandari ◽

Perry R. Mitchell ◽

Gabriela Suarez ◽

Neel B. Patel ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Fatty Acid ◽

Apis Mellifera ◽

Chemical Synthesis ◽

Acid Amide ◽

Fatty Acid Amides ◽

Fatty Acid Amide

Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. The fatty acid amidomes from mouse N18TG2 cells, sheep choroid plexus cells, Drosophila melanogaster, Bombyx mori, Apis mellifera, and Tribolium castaneum are presented.

Download Full-text

GC Analysis of Primary Fatty Acid Amides in Animal Fat

European Journal of Lipid Science and Technology ◽

10.1002/ejlt.202000323 ◽

2021 ◽

pp. 2000323

Author(s):

Milica Jovanovic ◽

Sigurd Schober ◽

Martin Mittelbach

Keyword(s):

Fatty Acid ◽

Gc Analysis ◽

Fatty Acid Amides ◽

Animal Fat

Download Full-text

Mechanical characterization of porous asphalt mixes modifying the asphalt with fatty acid amides -FAA-

Ingeniería e Investigación ◽

10.15446/ing.investig.v37n1.57158 ◽

2017 ◽

Vol 37 (1) ◽

pp. 43 ◽

Cited By ~ 3

Author(s):

Vanessa Senior Arrieta ◽

Jorge Eliecer Córdoba Maquilon

Keyword(s):

Fatty Acid ◽

Mechanical Characterization ◽

Experimental Methodology ◽

Chemical Additives ◽

Asphalt Mixes ◽

Porous Asphalt ◽

Fatty Acid Amides ◽

Pavement Structures ◽

And Performance

Porous asphalt mixes (PAM), form a special road surface for asphalt pavement structures, have a special particle size distribution that lets infiltrate to the runoff storm water through of it because of its voids content about 20 %. Many researchers conducted studies and have concluded that the use of modified asphalts is completely necessary to design PAM. Organic and chemical additives and special procedures as foamed asphalt have enhanced the performance of PAM, during their service life. This paper is focused on the mechanical characterization of PAM and how the asphalt modified with fatty acid amides, influenced on their behavior and performance. Based on an experimental methodology with laboratory tests aimed at establishing a comparison between porous asphalt mixes, using for its design and production a penetration 60-70 pure asphalt and another one asphalt modified with fatty acid amides.

Download Full-text

Mechanical properties of polymer-talc binary composite systems: effects of fatty acid amides.

NIPPON GOMU KYOKAISHI ◽

10.2324/gomu.66.724 ◽

1993 ◽

Vol 66 (10) ◽

pp. 724-730

Author(s):

Osamu SHIMOMURA ◽

Yoshio TAKAI ◽

Masami SAWADA ◽

Shigetoshi TAKAHASHI ◽

Kunio GOTO

Keyword(s):

Mechanical Properties ◽

Fatty Acid ◽

Fatty Acid Amides ◽

Composite Systems ◽

Binary Composite

Download Full-text

Molecular Mechanism of Physical Gelation of Hydrocarbons by Fatty Acid Amides of Natural Amino Acids.

ChemInform ◽

10.1002/chin.200739203 ◽

2007 ◽

Vol 38 (39) ◽

Author(s):

Asish Pal ◽

Yamuna K. Ghosh ◽

Santanu Bhattacharya

Keyword(s):

Amino Acids ◽

Fatty Acid ◽

Molecular Mechanism ◽

Fatty Acid Amides ◽

Physical Gelation ◽

Natural Amino Acids

Download Full-text

Acute postprandial effect of hydrogenated fish oil, palm oil and lard on plasma cholesterol, triacylglycerol and non-esterified fatty acid metabolism in normocholesterolaemic males

British Journal Of Nutrition ◽

10.1079/bjn20051723 ◽

2006 ◽

Vol 95 (4) ◽

pp. 787-794 ◽

Cited By ~ 4

Author(s):

Marie M. Cantwell ◽

Mary A.T. Flynn ◽

Michael J. Gibney

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Palm Oil ◽

Unsaturated Fatty Acids ◽

Lipid Analysis ◽

Plasma Cholesterol ◽

Random Order ◽

Postprandial Response ◽

Chain Fatty Acids

The majority of research has focused on the association betweentransunsaturated fatty acids (TUFA) from hydrogenated vegetable oils and heart disease even though TUFA are also produced from hydrogenated fish oil. We compared the acute effect of three solid fats on postprandial cholesterol, triacylglycerol (TAG) and NEFA concentrations in normocholesterolaemic males. Eight healthy male volunteers consumed each of the three 40g fat meals (partially hydrogenated fish oil (PHFO), palm oil and lard) in random order and blood samples were drawn at 2, 4, 6 and 8h thereafter for lipid analysis. The postprandial response in plasma TAG, TAG-rich lipoprotein-TAG (TRL-TAG), total cholesterol and plasma NEFA, measured as the area under the postprandial curve, was not significantly different between the three meals (p>0·05), which varied in MUFA, PUFA and TUFA content. There was no marked elevation of longer-chain fatty acids (C20–22,cisortransisomers) into the TRL-TAG fraction following the PHFO meal even though they provided 40% of the total fatty acids in the PHFO meal. The postprandial TRL-TAG response to PHFO was expected to be higher, as it is higher in TUFA, lower in PUFA and similar in saturated fatty acid composition compared with the lard and palm oil test meals. The absence of a higher postprandial response following ingestion of PHFO could be as a result of reduced absorption and increased oxidation of long-chain fatty acids (bothcisandtransisomers)

Download Full-text