3D Printed Monolithic Microreactors for Real-Time Detection of Klebsiella pneumoniae and the Resistance Gene blaNDM-1 by Recombinase Polymerase Amplification

We investigate the compatibility of three 3D printing materials towards real-time recombinase polymerase amplification (rtRPA). Both the general ability of the rtRPA reaction to occur while in contact with the cured 3D printing materials as well as the residual autofluorescence and fluorescence drift in dependence on post curing of the materials is characterized. We 3D printed monolithic rtRPA microreactors and subjected the devices to different post curing protocols. Residual autofluorescence and drift, as well as rtRPA kinetics, were then measured in a custom-made mobile temperature-controlled fluorescence reader (mTFR). Furthermore, we investigated the effects of storage on the devices over a 30-day period. Finally, we present the single- and duplex rtRPA detection of both the organism-specific Klebsiella haemolysin (khe) gene and the New Delhi metallo-β-lactamase 1 (blaNDM-1) gene from Klebsiella pneumoniae. Results: No combination of 3D printing resin and post curing protocol completely inhibited the rtRPA reaction. The autofluorescence and fluorescence drift measured were found to be highly dependent on printing material and wavelength. Storage had the effect of decreasing the autofluorescence of the investigated materials. Both khe and blaNDM-1 were successfully detected by single- and duplex-rtRPA inside monolithic rtRPA microreactors printed from NextDent Ortho Clear (NXOC). The reaction kinetics were found to be close to those observed for rtRPA performed in a microcentrifuge tube without the need for mixing during amplification. Singleplex assays for both khe and blaNDM-1 achieved a limit of detection of 2.5 × 101 DNA copies while the duplex assay achieved 2.5 × 101 DNA copies for khe and 2.5 × 102 DNA copies for blaNDM-1. Impact: We expand on the state of the art by demonstrating a technology that can manufacture monolithic microfluidic devices that are readily suitable for rtRPA. The devices exhibit very low autofluorescence and fluorescence drift and are compatible with RPA chemistry without the need for any surface pre-treatment such as blocking with, e.g., BSA or PEG.

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3D Printed Clamps for In Vitro Tensile Tests of Human Gracilis and the Superficial Third of Quadriceps Tendons

Applied Sciences ◽

10.3390/app11062563 ◽

2021 ◽

Vol 11 (6) ◽

pp. 2563

Author(s):

Ivan Grgić ◽

Vjekoslav Wertheimer ◽

Mirko Karakašić ◽

Željko Ivandić

Keyword(s):

3D Printing ◽

Fused Deposition Modeling ◽

Tensile Tests ◽

Biomechanical Properties ◽

Testing Procedure ◽

Laboratory Equipment ◽

Fused Deposition ◽

Custom Made ◽

3D Printed

Recent soft tissue studies have reported issues that occur during experimentation, such as the tissue slipping and rupturing during tensile loads, the lack of standard testing procedure and equipment, the necessity for existing laboratory equipment adaptation, etc. To overcome such issues and fulfil the need for the determination of the biomechanical properties of the human gracilis and the superficial third of the quadriceps tendons, 3D printed clamps with metric thread profile-based geometry were developed. The clamps’ geometry consists of a truncated pyramid pattern, which prevents the tendons from slipping and rupturing. The use of the thread application in the design of the clamp could be used in standard clamping development procedures, unlike in previously custom-made clamps. Fused deposition modeling (FDM) was used as a 3D printing technique, together with polylactic acid (PLA), which was used as a material for clamp printing. The design was confirmed and the experiments were conducted by using porcine and human tendons. The findings justify the usage of 3D printing technology for parts manufacturing in the case of tissue testing and establish independence from the existing machine clamp system, since it was possible to print clamps for each prepared specimen and thus reduce the time for experiment setup.

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Designer Cranioplasty at Budget Prices: A Novel Use of 3D Printing Technology

Indian Journal of Neurosurgery ◽

10.1055/s-0040-1716995 ◽

2021 ◽

Author(s):

Thirumal Yerragunta ◽

Reddy Ramanadha Kanala ◽

Vamsi Krishna Yerramneni ◽

Swapnil Kolpakawar ◽

Vasundhara Rangan

Keyword(s):

Patient Satisfaction ◽

3D Printing ◽

Bone Cement ◽

Polymethyl Methacrylate ◽

Printing Technology ◽

3D Printing Technology ◽

Pmma Bone Cement ◽

Custom Made ◽

3D Printed ◽

Exact Shape

Abstract Background Cranioplasty using synthetic materials for restoration of the exact shape of the skull has always remained a challenge until the development of 3D printing technology. However, the high-cost of available 3D printed implants limits their extensive use. Objectives To study the effectiveness of a low-cost, 3D-printed template for molding the polymethyl methacrylate (PMMA) (bone cement) in order to achieve exact contours of the skull specific to each patient. Materials and Methods 10 cranioplasties have been performed between July 2018 to December 2019 in a variety of craniotomy defects using bone cement flaps shaped using custom-made molds. The mold was 3D-printed and based on each patient’s CT images in digital imaging and communications in medicine (DICOM). Miniplates and screws were used to fix the flap. Postoperatively, clinical and radiological evaluation were done to assess patient satisfaction and accuracy of contour achieved. Results Patient satisfaction as well as accuracy of contouring, as seen on postoperative CT scans, were excellent. There were no notable complications on follow-up. Conclusion PMMA cranioplasty flap, contoured using a 3D-printed mold, is a very cost-effective alternative for restoration of skull contour for various craniotomy defects.Polymethyl methacrylate (PMMA) molded to form the exact shape of lost calvarium using 3D printed plastic templates is a smart and economical solution

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3D-printed devices for continuous-flow organic chemistry

Beilstein Journal of Organic Chemistry ◽

10.3762/bjoc.9.109 ◽

2013 ◽

Vol 9 ◽

pp. 951-959 ◽

Cited By ~ 110

Author(s):

Vincenza Dragone ◽

Victor Sans ◽

Mali H Rosnes ◽

Philip J Kitson ◽

Leroy Cronin

Keyword(s):

Organic Chemistry ◽

3D Printing ◽

Continuous Flow ◽

Flow Chemistry ◽

Flow Cell ◽

Proof Of Concept ◽

Time Analysis ◽

Real Time Analysis ◽

Custom Made ◽

3D Printed

We present a study in which the versatility of 3D-printing is combined with the processing advantages of flow chemistry for the synthesis of organic compounds. Robust and inexpensive 3D-printed reactionware devices are easily connected using standard fittings resulting in complex, custom-made flow systems, including multiple reactors in a series with in-line, real-time analysis using an ATR-IR flow cell. As a proof of concept, we utilized two types of organic reactions, imine syntheses and imine reductions, to show how different reactor configurations and substrates give different products.

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Development of a 3D Printed Double-Acting Linear Pneumatic Actuator for the Tendon Gripping

Polymers ◽

10.3390/polym13152528 ◽

2021 ◽

Vol 13 (15) ◽

pp. 2528

Author(s):

Ivan Grgić ◽

Vjekoslav Wertheimer ◽

Mirko Karakašić ◽

Željko Ivandić

Keyword(s):

3D Printing ◽

Fused Deposition Modeling ◽

Testing Machine ◽

Pneumatic Actuator ◽

Tensile Testing Machine ◽

Laboratory Equipment ◽

Testing Procedures ◽

Fused Deposition ◽

Custom Made ◽

3D Printed

The lack of standardization in tissue testing procedures results in a variety of custom-made devices. In the case of the determination of the mechanical properties of tendons, it is sometimes necessary to adapt the existing laboratory equipment for conducting experiments when specific commercial equipment is not applicable to solve issues such as proper gripping to prevent tendon slipping and rupturing, gripping control and manoeuvrability in case of tendon submerging and without contamination of the testing liquid. This paper presents the systematic development, design, and fabrication using 3D printing technology and the application of the double-acting linear pneumatic actuator to overcome such issues. It is designed to do its work submerged in the Ringers’ solution while gripping the tendon along with the clamps. The pneumatic foot valve unit of the Shimadzu AGS-X tensile testing machine controls the actuator thus preventing Ringers’ solution to be contaminated by the machine operator during specimen set-up. The actuator has a length of 60 mm, a bore of 50 mm, and a stroke length of 20 mm. It is designed to operate with an inlet pressure of up to 0.8 MPa. It comprises the cylinder body with the integrated thread, the piston, the piston head, and the gripper jaw. Fused deposition modeling (FDM) has been used as the 3D printing technique, along with polylactic acid (PLA) as the material for 3D printing. The 3D printed double-acting linear pneumatic actuator was developed into an operating prototype. This study could open new frontiers in the field of tissue testing and the development of similar specialized devices for medical purposes.

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Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae

10.21203/rs.3.rs-17865/v2 ◽

2020 ◽

Author(s):

Jinfeng Wang ◽

Ruiwen Li ◽

Xiaoxia Sun ◽

Libing Liu ◽

Xuepiao Hao ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Limit Of Detection ◽

Lateral Flow ◽

Recombinase Polymerase Amplification ◽

Metal Bath ◽

Lateral Flow Strip ◽

The Real ◽

Mycoplasmal Pneumonia ◽

Mycoplasma Ovipneumoniae

Abstract Background: Mycoplasmal pneumonia is an important infectious disease that threatens sheep and goat production worldwide, and Mycoplasma ovipneumoniae is one of major etiological agent causing mycoplasmal pneumonia. Recombinase polymerase amplification (RPA) is an isothermal nucleic acid amplification technique, and RPA-based diagnostic assays have been described for the detection of different types of pathogens. Results: The RPA assays using real-time fluorescence detection (real-time RPA) and lateral flow strip detection (LFS RPA) were developed to detect M. ovipneumoniae targeting a conserved region of the 16S rRNA gene. Real-time RPA was performed in a portable florescence scanner at 39 °C for 20 min. LFS RPA was performed in a portable metal bath incubator at 39 °C for 15 min, and the amplicons were visualized with the naked eyes within 5 min on the lateral flow strip. Both assays were highly specific for M. ovipneumoniae , as there were no cross-reactions with other microorganisms tested, especially the pathogens involved in respiratory complex and other mycoplasmas frequently identified in ruminant s . The limit of detection of LFS RPA assay was 1.0×10 1 copies per reaction using a recombinant plasmid containing target gene as template, which is 10 times lower than the limit of detection of the real-time RPA and real-time PCR assays. The RPA assays were further validated on 111 clinical sheep nasal swab and fresh lung samples, and M. ovipneumoniae DNA was detected in 29 samples in the real-time RPA, 31 samples in the LFS RPA and 32 samples in the real-time PCR assay. Compared to real-time PCR, the real-time RPA and LFS RPA showed diagnostic specificity of 100% and 98.73%, diagnostic sensitivity of 90.63% and 93.75%, and a kappa coefficient of 0.932 and 0.934, respectively. Conclusions: The developed real-time RPA and LFS RPA assays provide the attractive and promising tools for rapid, convenient and reliable detection of M. ovipneumoniae , especially in resource-limited settings.

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Benefits And Limitations Of Three-Dimensional Printing Technology For Ecological Research

10.1101/283895 ◽

2018 ◽

Cited By ~ 1

Author(s):

Jocelyn E. Behm ◽

Brenna R. Waite ◽

S. Tonia Hsieh ◽

Matthew R. Helmus

Keyword(s):

3D Printing ◽

New Technology ◽

Wood Fiber ◽

Three Dimensional ◽

Model Organisms ◽

Successful Implementation ◽

Ecological Research ◽

Predator Attack ◽

Custom Made ◽

3D Printed

AbstractBackgroundEcological research often involves sampling and manipulating non-model organisms that reside in heterogeneous environments. As such, ecologists often adapt techniques and ideas from industry and other scientific fields to design and build equipment, tools, and experimental contraptions custom-made for the ecological systems under study. Three-dimensional (3D) printing provides a way to rapidly produce identical and novel objects that could be used in ecological studies, yet ecologists have been slow to adopt this new technology. Here, we provide ecologists with an introduction to 3D printing.ResultsFirst, we give an overview of the ecological research areas in which 3D printing is predicted to be the most impactful and review current studies that have already used 3D printed objects. We then outline a methodological workflow for integrating 3D printing into an ecological research program and give a detailed example of a successful implementation of our 3D printing workflow for 3D printed models of the brown anole, Anolis sagrei, for a field predation study. After testing two print media in the field, we show that the models printed from the less expensive and more sustainable material (blend of 70% plastic and 30% recycled wood fiber) were just as durable and had equal predator attack rates as the more expensive material (100% virgin plastic).ConclusionsOverall, 3D printing can provide time and cost savings to ecologists, and with recent advances in less toxic, biodegradable, and recyclable print materials, ecologists can choose to minimize social and environmental impacts associated with 3D printing. The main hurdles for implementing 3D printing – availability of resources like printers, scanners, and software, as well as reaching proficiency in using 3D image software – may be easier to overcome at institutions with digital imaging centers run by knowledgeable staff. As with any new technology, the benefits of 3D printing are specific to a particular project, and ecologists must consider the investments of developing usable 3D materials for research versus other methods of generating those materials.

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3D printing of metallic micro-gears for micro-fluidic applications

Journal of Micromechanics and Molecular Physics ◽

10.1142/s2424913021410022 ◽

2021 ◽

Author(s):

C. Wang ◽

S. Chandra ◽

X. P. Tan ◽

S. B. Tor

Keyword(s):

3D Printing ◽

Three Dimensional ◽

Stainless Steel 316L ◽

Promising Alternative ◽

Powder Bed ◽

Micro Scale ◽

Direct Fabrication ◽

Custom Made ◽

Fluidic Devices ◽

3D Printed

Micro-fluidic devices are essential to handle fluids on the micro-meter scale (micro-scale), making them crucial to biomedical applications, where micro-gear is the key component for active fluid mixing. Rapid and direct fabrication of micro-gears is preferred because they are usually custom-made to specific applications and iterative design is needed. However, conventional manufacturing (CM) techniques for micro-fluidic devices are labor-intensive and time-consuming as multiple steps are required. Three-dimensional (3D) printing, or formally known as additive manufacturing (AM) offers a promising alternative over CM techniques in producing near-net shape complex geometries, given the micro-scale fabrication process. In this work, two types of powder-bed fusion (PBF) AM techniques, namely laser-PBF (L-PBF) and electron beam-PBF (EB-PBF) are used to benchmark 3D-printed micro-gears from stainless steel 316L micro-granular powders. Results showcase the preeminence of L-PBF over EB-PBF in generating distinguishable micro-scale features on gear profiles and superior micro-hardness in mechanical property. Overall, PBF metal AM shows significant promise in advancing the otherwise tedious state of CM for micro-gears.

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Mallet Finger Lattice Casts Using 3D Printing

Journal of Healthcare Engineering ◽

10.1155/2019/4765043 ◽

2019 ◽

Vol 2019 ◽

pp. 1-5

Author(s):

Hyeunwoo Choi ◽

Anna Seo ◽

Jongmin Lee

Keyword(s):

Clinical Practice ◽

3D Printing ◽

Mallet Finger ◽

Printing Technology ◽

3D Printing Technology ◽

Plaster Of Paris ◽

Advantages And Disadvantages ◽

Appropriate Treatment ◽

Custom Made ◽

3D Printed

Currently, research based on the technology and applications of 3D printing is being actively pursued. 3D printing technology, also called additive manufacturing, is widely and increasingly used in the medical field. This study produced custom casts for the treatment of mallet finger using plaster of Paris, which was traditionally used in clinical practice, and 3D printing technology, and evaluated their advantages and disadvantages for patients by conducting a wearability assessment. Mallet finger casts produced using plaster of Paris, when incorrectly made, can result in skin necrosis and other problems for patients. These problems can be mitigated, however, by creating casts using 3D printing technology. Additionally, plaster casts or ready-made alternatives can be inconvenient with respect to rapid treatment of patients. In contrast, 3D-printed casts appear to provide patients with appropriate treatment and increase their satisfaction because they are small in size, custom-made for each patient, and can be quickly made and immediately applied in clinical practice.

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Custom-made artificial eyes using 3D printing for dogs: A preliminary study

PLoS ONE ◽

10.1371/journal.pone.0242274 ◽

2020 ◽

Vol 15 (11) ◽

pp. e0242274

Author(s):

So-Young Park ◽

Jeong-Hee An ◽

Hyun Kwon ◽

Seo-Young Choi ◽

Ka-Young Lim ◽

...

Keyword(s):

3D Printing ◽

Computer Aided Design ◽

Companion Animals ◽

Manufacturing Cost ◽

Artificial Eye ◽

Custom Made ◽

3D Printed ◽

Preliminary Study ◽

Ocular Implant

Various incurable eye diseases in companion animals often result in phthisis bulbi and eye removal surgery. Currently, the evisceration method using silicone balls is useful in animals; however, it is not available to those with impaired cornea or severe ocular atrophy. Moreover, ocular implant and prostheses are not widely used because of the diversity in animal size and eye shape, and high manufacturing cost. Here, we produced low-cost and customized artificial eyes, including implant and prosthesis, using computer-aided design and three-dimensional (3D) printing technique. For 3D modeling, the size of the artificial eyes was optimized using B-mode ultrasonography. The design was exported to STL files, and then printed using polycaprolactone (PCL) for prosthesis and mixture of PCL and hydroxyapatite (HA) for ocular implant. The 3D printed artificial eyes could be produced in less than one and half hour. The prosthesis was painted using oil colors and biocompatible resin. Two types of eye removal surgery, including evisceration and enucleation, were performed using two beagle dogs, as a preliminary study. After the surgery, the dogs were clinically evaluated for 6 months and then histopathological evaluation of the implant was done. Ocular implant was biocompatible and host tissue ingrowth was induced after in vivo application. The custom-made prosthesis was cosmetically excellent. Although long-term clinical follow-up might be required, the use of 3D printed-customized artificial eyes may be beneficial for animals that need personalized artificial eye surgery.

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DESIGN, MANUFACTURING AND PROBLEM ANALYSIS OF AN ENTIRELY FDM 3D PRINTED LINEAR PNEUMATIC ACTUATOR

TECHNICAL SCIENCES AND TECHNOLOG IES ◽

10.25140/2411-5363-2020-3(21)-98-105 ◽

2020 ◽

pp. 98-105

Author(s):

Martin Varga ◽

Filip Filakovský

Keyword(s):

3D Printing ◽

Low Cost ◽

Pneumatic Actuator ◽

Problem Analysis ◽

Pneumatic Actuators ◽

Manufacturing Plants ◽

Custom Made ◽

Machine Shops ◽

3D Printed ◽

Linear Actuators

Urgency of the research. Currently, manufacturing of pneumatic components is reserved only to well-equipped manufacturing plants and machine shops. The ability to reliably manufacture pneumatic components on a FDM 3D printer would enable the creation of low-cost custom-made pneumatic actuators with novel properties. This, in turn, could increase the speed and lower the cost of development of prototypes that use pressure air as their power source. Target setting. Today cost-effective 3D printers can be found both in manufacturing plants and small machine shops andhobbyist workshops. Hawing the possibility to make reliable pneumatic components like pneumatic actuators on such machines could be beneficial and lead to opening new applications for them. Actual scientific researches and issues analysis. Currently most research on using additive manufacturing to construct a pneumatic actuator focuses mainly on bellows type actuators. Research on 3d printing of classical pneumatic actuators is scarce and often presents a rough overview of the design process and immediately presenting a functional prototype without focusing and studying the design hurdles thoroughly. Uninvestigated parts of general matters defining. Parts for firm pneumatic actuators manufactured by FDM 3D printing exhibit properties that have detrimental effects on the optimal working of such actuators. The question is on the magnitude of these effects whether these effects can be tolerated and how to design such a firm pneumatic actuator without the need to postprocess all the components. The research objective. The aim of these research was to manufacture an early prototype of full plastic 3d printed not postprocessed linear actuators and make a preliminary analysis of encountered problems therefore pointing the way for further research in this field. The statement of basic materials. The analysis consists of an attempt to manufacture a simple prototype of full plastic 3d printed linear actuators without the use of postprocessing techniques and establishing a baseline for further research. Conclusions. In this paper the design of three iterations of FDM 3D printed pneumatic cylinders are presented. The problems arising from not using any postprocessing on either of the parts and using only 3d printed parts for the construction is also discussed. In the final chapter the design hurdles for the design and manufacturing of such an actuator are presented..

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