scholarly journals A Continuous Cell Separation and Collection Approach on a Microfilter and Negative Dielectrophoresis Combined Chip

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1037
Author(s):  
Qiong Wang ◽  
Xiaoling Zhang ◽  
Danfen Yin ◽  
Jinan Deng ◽  
Jun Yang ◽  
...  
Keyword(s):  
Analytical Chemistry ◽  
Inclination Angle ◽  
Microfluidic Chip ◽  
Biological Samples ◽  
Cell Separation ◽  
Collection Efficiency ◽  
Good Separation ◽  
Electrode Distance

Cell separation plays an important role in the fields of analytical chemistry and biomedicine. To solve the blockage problem and improve the separation throughput in the traditional microstructure filtration-based separation approach, a continuous cell separation and collection approach via micropost array railing on a microfilter and negative dielectrophoresis combined chip is proposed. By tilting the micropost array at a certain angle, microparticles or cells enter the collection area under micropost array railing. The effects of the inclination angle of the micropost array and the electrode distance on the microparticle collection efficiency were investigated. Based on the optimized microfluidic chip structure, 37- and 16.3-μm particles were collected with 85% and 89% efficiencies, respectively. Additionally, algal cells were separated and collected by using the optimized microchip. The chip also had good separation and collection effects on biological samples, which effectively solved the blockage problem and improved the separation throughput, laying a foundation for subsequent microstructure filtration separation-based research and application.

scholarly journals Analytical sonochemistry; developments, applications, and hyphenations of ultrasound in sample preparation and analytical techniques

2012 ◽  
Vol 10 (4) ◽  
pp. 938-976 ◽  
Author(s):  
Shahram Seidi ◽  
Yadollah Yamini
Keyword(s):  
Analytical Chemistry ◽  
Sample Preparation ◽  
Biological Samples ◽  
Inorganic Compounds ◽  
Analytical Techniques ◽  
Theoretical Understanding ◽  
Environmental And Biological Samples ◽  
Modern Analytical ◽  
Soil And Water

AbstractUltrasonic assistance is one of the great successes of modern analytical chemistry, which uses this energy for a variety of purposes in relation to sample preparation and development of methods for the analysis of numerous contaminants including organic and inorganic compounds. This review will attempt to provide an overview of more recent applications of ultrasound in different environmental and biological samples such as food, soil and water as well as a brief description of the theoretical understanding of this method. Also, the possibility of coupling ultrasound with other analytical techniques will be discussed.

Chemiluminescence determination of moxifloxacin in pharmaceutical and biological samples based on its enhancing effect of the luminol-ferricyanide system using a microfluidic chip

Luminescence ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 248-253 ◽  
Author(s):  
Yeoun Suk Suh ◽  
Mohammad Kamruzzaman ◽  
Al-Mahmnur Alam ◽  
Sang Hak Lee ◽  
Young Ho Kim ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Biological Samples ◽  
Enhancing Effect

scholarly journals Characterisation of a new online nanoLC-CZE-MS platform and application for the glycosylation profiling of alpha-1-acid glycoprotein

2021 ◽  
Author(s):  
Alexander Stolz ◽  
Christian Neusüß
Keyword(s):  
Biological Samples ◽  
High Sensitivity ◽  
Good Separation ◽  
General Applicability ◽  
Intact Proteins ◽  
Acid Glycoprotein ◽  
Zone Electrophoresis ◽  
Concentration Sensitivity ◽  
Capillary Zone ◽  
High Degree

AbstractThe ever-increasing complexity of biological samples to be analysed by mass spectrometry has led to the necessity of sophisticated separation techniques, including multidimensional separation. Despite a high degree of orthogonality, the coupling of liquid chromatography (LC) and capillary zone electrophoresis (CZE) has not gained notable attention in research. Here, we present a heart-cut nanoLC-CZE-ESI-MS platform to analyse intact proteins. NanoLC and CZE-MS are coupled using a four-port valve with an internal nanoliter loop. NanoLC and CZE-MS conditions were optimised independently to find ideal conditions for the combined setup. The valve setup enables an ideal transfer efficiency between the dimensions while maintaining good separation conditions in both dimensions. Due to the higher loadability, the nanoLC-CZE-MS setup exhibits a 280-fold increased concentration sensitivity compared to CZE-MS. The platform was used to characterise intact human alpha-1-acid glycoprotein (AGP), an extremely heterogeneous N-glycosylated protein. With the nanoLC-CZE-MS approach, 368 glycoforms can be assigned at a concentration of 50 μg/mL as opposed to the assignment of only 186 glycoforms from 1 mg/mL by CZE-MS. Additionally, we demonstrate that glycosylation profiling is accessible for dried blood spot analysis (25 μg/mL AGP spiked), indicating the general applicability of our setup to biological matrices. The combination of high sensitivity and orthogonal selectivity in both dimensions makes the here-presented nanoLC-CZE-MS approach capable of detailed characterisation of intact proteins and their proteoforms from complex biological samples and in physiologically relevant concentrations. Graphical abstract

A one-step molded microfluidic chip featuring a two-layer silver-PDMS microelectrode for dielectrophoretic cell separation

The Analyst ◽  
2020 ◽  
Vol 145 (16) ◽  
pp. 5603-5614 ◽  
Author(s):  
Zhongle Zhang ◽  
Yuan Luo ◽  
Xiaofeng Nie ◽  
Duli Yu ◽  
Xiaoxing Xing
Keyword(s):  
Microfluidic Chip ◽  
Cell Separation ◽  
Molding Process ◽  
Replica Molding ◽  
Viability Assay ◽  
One Step

Microelectrodes and flow sidewalls entirely made of AgPDMS by one-step replica molding process for dielectrophoretic cell separation and viability assay.

scholarly journals A Rapid Detection Method for Tomato Gray Mold Spores in Greenhouse Based on Microfluidic Chip Enrichment and Lens-Less Diffraction Image Processing

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3011
Author(s):  
Yafei Wang ◽  
Hanping Mao ◽  
Xiaodong Zhang ◽  
Yong Liu ◽  
Xiaoxue Du
Keyword(s):  
Image Processing ◽  
Botrytis Cinerea ◽  
Microfluidic Chip ◽  
Rapid Detection ◽  
Detection Method ◽  
Collection Efficiency ◽  
Gray Mold ◽  
Diffraction Image ◽  
Manual Counting ◽  
Chip Enrichment

It is of great significance to find tomato gray mold in time and take corresponding control measures to ensure the production of tomato crops. This study proposed a rapid detection method for spores of Botrytis cinerea in green-house based on microfluidic chip enrichment and lens-free diffraction image processing. Microfluidic chip with a regular triangular inner rib structure was designed to achieve the enrichment of Botrytis cinerea spores. In order to obtain the diffraction image of the diseased spores, a lens-less diffraction imaging system was built. Furthermore, the collected spore diffraction images were processed and counted. The simulation results showed that the collection efficiency of 16 μm particles was 79%, 100%, and 89% at the inlet flow rate of 12, 14 and 16 mL/min, respectively. The experimental verification results were observed under a microscope. The results showed that when the flow rate of the microfluidic chip was 12, 14 and 16 mL/min, the collection efficiency of Botrytis cinerea spores was 70.65%, 87.52% and 77.96%, respectively. The Botrytis cinerea spores collected in the experiment were placed under a microscope for manual counting and compared with the automatic counting results based on diffraction image processing. A total of 10 sets of experiments were carried out, with an error range of the experiment was 5.13~8.57%, and the average error of the experiment was 6.42%. The Bland–Altman method was used to analyze two methods based on diffraction image processing and manual counting under a microscope. All points are within the 95% consistency interval. Therefore, this study can provide a basis for the research on the real-time monitoring technology of tomato gray mold spores in the greenhouse.

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