DNA Studies: Latest Spectroscopic and Structural Approaches

This review looks at the different approaches, techniques, and materials devoted to DNA studies. In the past few decades, DNA nanotechnology, micro-fabrication, imaging, and spectroscopies have been tailored and combined for a broad range of medical-oriented applications. The continuous advancements in miniaturization of the devices, as well as the continuous need to study biological material structures and interactions, down to single molecules, have increase the interdisciplinarity of emerging technologies. In the following paragraphs, we will focus on recent sensing approaches, with a particular effort attributed to cutting-edge techniques for structural and mechanical studies of nucleic acids.

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Lighting Up RNA-Cleaving DNAzymes for Biosensing

Journal of Nucleic Acids ◽

10.1155/2012/958683 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 14

Author(s):

Kha Tram ◽

Pushpinder Kanda ◽

Yingfu Li

Keyword(s):

Nucleic Acids ◽

Random Sequence ◽

Dna Nanotechnology ◽

Research Topic ◽

Selection Technique ◽

Fluorescent Reporters ◽

The Past ◽

Catalytic Dna ◽

Functional Nucleic Acids

The development of thein vitroselection technique has allowed the isolation of functional nucleic acids, including catalytic DNA molecules (DNAzymes), from random-sequence pools. The first-ever catalytic DNA obtained by this technique in 1994 is a DNAzyme that cleaves RNA. Since then, many other RNase-like DNAzymes have been reported from multiplein vitroselection studies. The discovery of various RNase DNAzymes has in turn stimulated the exploration of these enzymatic species for innovative applications in many different areas of research, including therapeutics, biosensing, and DNA nanotechnology. One particular research topic that has received considerable attention for the past decade is the development of RNase DNAzymes into fluorescent reporters for biosensing applications. This paper provides a concise survey of the most significant achievements within this research topic.

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Albumins as Embedding Media for Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100064025 ◽

1969 ◽

Vol 27 ◽

pp. 422-423 ◽

Cited By ~ 1

Author(s):

J. L. Farrant ◽

J. D. McLean

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

High Temperature ◽

Biological Material ◽

Globular Proteins ◽

The Past ◽

Antibody Staining ◽

Thin Sectioning ◽

Embedding Medium

For electron microscope techniques such as ferritin-labeled antibody staining it would be advantageous to have available a simple means of thin sectioning biological material without subjecting it to lipid solvents, impregnation with plastic monomers and their subsequent polymerization. With this aim in view we have re-examined the use of protein as an embedding medium. Gelatin which has been used in the past is not very satisfactory both because of its fibrous nature and the high temperature necessary to keep its solutions fluid. We have found that globular proteins such as the serum and egg albumins can be cross-linked so as to yield blocks which are suitable for ultrathin sectioning.

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STEM for All on the SS Freshspring: VR Tour

10.3940/rina.hist.20.05 ◽

2020 ◽

Author(s):

Hashim Yaqub ◽

Martin Kemp

Keyword(s):

Fresh Water ◽

Cutting Edge ◽

Future Generations ◽

Extensive Experience ◽

Wide Audience ◽

Virtual Tour ◽

The Past ◽

Engineering And Technology ◽

The World ◽

Interactive 3D

Over the past year the world has changed dramatically. With greater restrictions on accessibility, the need to provide innovative and distributable remote experiences is now more prominent than ever. BMT has partnered with the SS Freshspring Trust to create multi-generational STEM experiences. “Preserving the past to inspire knowledge for the future”. The SS Freshspring Trust have a vision to become a STEM hub by utilising cutting-edge technology. BMT have extensive experience in developing VR applications in the Maritime Domain. With skills shortfalls in many engineering disciplines, there is a need to inspire future generations into careers in STEM. Equally, many adults have a passion for technology and have valuable skills to offer to STEM projects. This paper uses the historic vessel SS Freshspring, a 1940s RFA Fresh Water Carrier currently being restored in North Devon, as the basis for exploring a range of initiatives and activities aimed at making engineering and technology interesting and accessible to all. The specific focus is on the development of an interactive 3D virtual tour, aiming to provide access to a wide audience by targeting a range of modalities including smartphones, internet browsers, and most consumer VR headsets.

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Nuevos y viejos campos para el estudio de la guerra a lo largo del siglo XX: un motor de innovación historiográfica

HISPANIA NOVA Primera Revista de Historia Contemporánea on-line en castellano Segunda Época ◽

10.20318/hn.2018.4035 ◽

2018 ◽

pp. 164

Author(s):

David Alegre Lorenz

Keyword(s):

Gender Studies ◽

Social History ◽

Critical Review ◽

Great Influence ◽

Military History ◽

Cutting Edge ◽

International Level ◽

Total War ◽

The Past ◽

Siglo Xx

Resumen: Este artículo tiene por objeto analizar los principales debates y avances en uno de los campos más punteros y prolíficos de la historiografía a nivel internacional: los estudios de la guerra, también conocidos como nueva historia militar. Para ello propongo un recorrido a través de los cambios que se han producido dentro de éste ámbito durante las dos últimas décadas, así como también un examen crítico de los trabajos y tendencias historiográficas que más han contribuido a ello. En este sentido, planteo una puesta en valor de los estudios de la guerra y destaco su importancia para el conjunto de la historiografía por su capacidad para complejizar nuestro conocimiento y explicaciones del pasado; por el amplio y sugerente abanico de casos de estudio que pone a nuestra disposición; por sus tremendas posibilidades y potencial renovador a nivel metodológico e interpretativo; y, no menos importante, por su tremenda actualidad y sus conexiones con el presente.Palabras clave: estudios de la guerra, nueva historia militar, historia social, estudios de género, estudios culturales, guerra total.Abstract: This article is intended to analyze the major discussions and developments in one of the most prolific and a cutting-edge historiographic field on an international level: the war studies, also known as new military history. For this reason I propose a look through the changes which have taken place in this area over the last two decades, as well as a critical review of the works and historiographical paradigms that have contributed in that way. In this sense I defend the value of war studies and its importance for the whole historiography, taking into account specially its capacity to enable a more complex understanding and explanation of the past; the wide and suggestive range of subject matters that place at our disposal; its huge possibilities and renewing potential on a methodological and interpretative level; and last but not least its great influence and connections with the present.Keywords: war studies, new military history, social history, gender studies, cultural studies, total war.

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Tourism entrepreneurship research: a perspective article

Tourism Review ◽

10.1108/tr-06-2019-0204 ◽

2019 ◽

Vol 75 (1) ◽

pp. 122-125

Author(s):

Vanessa Ratten

Keyword(s):

Design Methodology ◽

Emerging Technologies ◽

Past Research ◽

Research Interest ◽

Content Type ◽

Entrepreneurship Research ◽

The Past ◽

Area Of Study

Purpose Tourism entrepreneurship is an emerging area of study that has both practical and theoretical importance. This paper aims to review past research on tourism entrepreneurship with the view of highlighting neglected areas of study. Design/methodology/approach A review of the past 75 years is conducted that highlights the gaps in the research in need of further research. Findings There is a focus on lifestyle and sustainable forms of tourism entrepreneurship without taking into account emerging technologies and other forms of entrepreneurship such as digital and societal. Originality/value This paper places emphasis on the transdisciplinary nature of tourism entrepreneurship that enables researchers to build on multiple disciplines to derive fruitful new areas of research interest.

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A perspective on the cutting edge of world order inquiry: The past, present and future of WOMP

International Interactions ◽

10.1080/03050628108434573 ◽

1981 ◽

Vol 8 (1-2) ◽

pp. 151-160

Author(s):

Saul Mendlovitz

Keyword(s):

World Order ◽

Cutting Edge ◽

The Past

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Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA

Molecules ◽

10.3390/molecules24030491 ◽

2019 ◽

Vol 24 (3) ◽

pp. 491 ◽

Cited By ~ 8

Author(s):

Gurleen Kaur ◽

Jacob Lewis ◽

Antoine van Oijen

Keyword(s):

Mechanical Properties ◽

Nucleic Acids ◽

Small Molecules ◽

Single Molecule ◽

Single Molecules ◽

Dna Molecules ◽

Dna Interactions ◽

Biochemical Reactions ◽

Imaging Methods ◽

Protein Dna Interactions

The ability to watch single molecules of DNA has revolutionised how we study biological transactions concerning nucleic acids. Many strategies have been developed to manipulate DNA molecules to investigate mechanical properties, dynamics and protein–DNA interactions. Imaging methods using small molecules and protein-based probes to visualise DNA have propelled our understanding of complex biochemical reactions involving DNA. This review focuses on summarising some of the methodological developments made to visualise individual DNA molecules and discusses how these probes have been used in single-molecule biophysical assays.

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Electrochemical aptasensors for detection of small molecules, macromolecules, and cells

Reviews in Analytical Chemistry ◽

10.1515/revac-2016-0009 ◽

2016 ◽

Vol 35 (4) ◽

pp. 201-211 ◽

Cited By ~ 6

Author(s):

Kun Han ◽

Tao Liu ◽

Yuanhong Wang ◽

Peng Miao

Keyword(s):

Nucleic Acids ◽

Small Molecules ◽

Electrochemical Biosensors ◽

Future Perspectives ◽

High Affinity ◽

The Past ◽

Fast Development ◽

Efficient Recognition

AbstractAptamers are a kind of special nucleic acids that have the ability to bind various targets (e.g. small molecules, macromolecules, and cells) with high affinity and specificity. As a type of efficient recognition component in analytical designs and applications, aptamers have gained intense achievements in the designs of biosensors. Especially, the past few years have witnessed the fast development of electrochemical aptasensors. In this mini-review, we summarize recent progresses in electrochemical biosensors based on aptamers. Different targets and the corresponding detection principles are introduced. The methods to enhance detectable signals are mentioned including the employment of nanomaterials. Also, some possible limitations and future perspectives are discussed.

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The Nature of Confessional Authority

Scottish Journal of Theology ◽

10.1017/s0036930600024042 ◽

1971 ◽

Vol 24 (3) ◽

pp. 271-289

Author(s):

G. R. Ferguson

Keyword(s):

Cutting Edge ◽

Sharp Contrast ◽

Good News ◽

The Past ◽

Confessing Church ◽

New Situation

In a series of studies for the E.A.C.C. writers from the Asian Churches applied themselves to the question ‘Confessing the Faith in Asia Today’. One feature common to their concern was the need to distinguish sharply between a confessing church and a confessional church. In their missionary situation the second term was treated with suspicion and mistrust as an expression which characterised a church that was inflexible in its attitudes and was therefore unable to enter into a living dialogue of faith with its indigenous situation so that the Gospel could be meaningfully expressed. This sharp contrast is understandable since the Asian churches have not been notable for the ways in which the Gospel has become indigenous to their cultural situation. It has, in the past, tended to be an imposition on a new situation, of a relevant Gospel couched in alien terms with more than a suggestion that the terms in which the Gospel was expressed was to be identified with the reality of the Good News. As a result a cultural hiatus has ensued which has vitiated a great deal of the cutting edge of the Gospel.

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