scholarly journals Current Development in Interdigital Transducer (IDT) Surface Acoustic Wave Devices for Live Cell In Vitro Studies: A Review

Micromachines ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 30
Author(s):  
Mazlee Bin Mazalan ◽  
Anas Mohd Noor ◽  
Yufridin Wahab ◽  
Shuhaida Yahud ◽  
Wan Safwani Wan Kamarul Zaman
Keyword(s):  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Microfluidic System ◽  
Live Cell ◽  
Cell Manipulation ◽  
Live Cells ◽  
Future Application ◽  
Wide Range ◽  
Separation Cell

Acoustics have a wide range of uses, from noise-cancelling to ultrasonic imaging. There has been a surge in interest in developing acoustic-based approaches for biological and biomedical applications in the last decade. This review focused on the application of surface acoustic waves (SAW) based on interdigital transducers (IDT) for live-cell investigations, such as cell manipulation, cell separation, cell seeding, cell migration, cell characteristics, and cell behaviours. The approach is also known as acoustofluidic, because the SAW device is coupled with a microfluidic system that contains live cells. This article provides an overview of several forms of IDT of SAW devices on recently used cells. Conclusively, a brief viewpoint and overview of the future application of SAW techniques in live-cell investigations were presented.

scholarly journals A modular microfluidic system based on a multilayered configuration to generate large-scale perfusable microvascular networks

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Tao Yue ◽  
Da Zhao ◽  
Duc T. T. Phan ◽  
Xiaolin Wang ◽  
Joshua Jonghyun Park ◽  
...  
Keyword(s):  
Large Scale ◽  
Circulatory System ◽  
Vertical Direction ◽  
Microfluidic System ◽  
Vital Role ◽  
Microvascular Networks ◽  
Biological Studies ◽  
Wide Range ◽  
High Flexibility

AbstractThe vascular network of the circulatory system plays a vital role in maintaining homeostasis in the human body. In this paper, a novel modular microfluidic system with a vertical two-layered configuration is developed to generate large-scale perfused microvascular networks in vitro. The two-layer polydimethylsiloxane (PDMS) configuration allows the tissue chambers and medium channels not only to be designed and fabricated independently but also to be aligned and bonded accordingly. This method can produce a modular microfluidic system that has high flexibility and scalability to design an integrated platform with multiple perfused vascularized tissues with high densities. The medium channel was designed with a rhombic shape and fabricated to be semiclosed to form a capillary burst valve in the vertical direction, serving as the interface between the medium channels and tissue chambers. Angiogenesis and anastomosis at the vertical interface were successfully achieved by using different combinations of tissue chambers and medium channels. Various large-scale microvascular networks were generated and quantified in terms of vessel length and density. Minimal leakage of the perfused 70-kDa FITC-dextran confirmed the lumenization of the microvascular networks and the formation of tight vertical interconnections between the microvascular networks and medium channels in different structural layers. This platform enables the culturing of interconnected, large-scale perfused vascularized tissue networks with high density and scalability for a wide range of multiorgan-on-a-chip applications, including basic biological studies and drug screening.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

Effects of medium and hypothermic temperatures on preservation of isolated porcine testis cells

2010 ◽  
Vol 22 (3) ◽  
pp. 523 ◽  
Author(s):  
Yanfei Yang ◽  
Ali Honaramooz
Keyword(s):  
Trypan Blue ◽  
Cell Isolation ◽  
Live Cell ◽  
Live Cells ◽  
Cell Recovery ◽  
Trypan Blue Exclusion ◽  
Culture Studies ◽  
Porcine Testis ◽  
Peritubular Cells

The effects of medium and hypothermic temperatures on testis cells were investigated to develop a strategy for their short-term preservation. Testes from 1-week-old piglets were enzymatically dissociated for cell isolation. In Experiment 1, testis cells were stored at either room (RT) or refrigeration (RG) temperature for 6 days in one of 13 different media. Live cell recovery was assayed daily using trypan blue exclusion. In Experiment 2, three media at RG were selected for immunocytochemical and in vitro culture studies. Live cell recovery was also assayed daily for 6 days using both trypan blue exclusion and a fluorochrome assay kit. For all media tested, significantly or numerically more live cells were maintained at RG than RT. On preservation Day 3 at RG (cell isolation day as Day 0), 20% FBS-Leibovitz resulted in the highest live cell recovery (89.5 ± 1.7%) and DPBS in the lowest (60.3 ± 1.9%). On Day 6 at RG, 20% FBS- Leibovitz also resulted in the best preservation efficiency with 80.9 ± 1.8% of Day 0 live cells recovered. There was no difference in live cell recovery detected by the two viability assays. After preservation, the proportion of gonocytes did not change, whereas that of Sertoli and peritubular cells increased and decreased, respectively. After 6 days of hypothermic preservation, testis cells showed similar culture potential to fresh cells. These results show that testis cells can be preserved for 6 days under hypothermic conditions with a live cell recovery of more than 80% and after-storage viability of 88%.

scholarly journals Wide range of droplet jetting angles by thin-film based surface acoustic waves

2020 ◽  
Vol 53 (35) ◽  
pp. 355402 ◽  
Author(s):  
Jie Li ◽  
Mehdi H Biroun ◽  
Ran Tao ◽  
Yong Wang ◽  
Hamdi Torun ◽  
...  
Keyword(s):  
Thin Film ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Wide Range

Ligand-displacement-based two-photon fluorogenic probe for visualizing mercapto biomolecules in live cells, Drosophila brains and zebrafish

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3433-3441 ◽  
Author(s):  
Yanfei Zhao ◽  
Yun Ni ◽  
Liulin Wang ◽  
Chenchen Xu ◽  
Chenqi Xin ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Live Cell ◽  
Live Cells ◽  
Two Photon ◽  
Ligand Displacement ◽  
Cell Tissue ◽  
Fluorogenic Probe ◽  
Displacement Based

We report the Fe(iii)-based complex TPFeS which acts as a novel ligand-displacement-based TP fluorogenic probe for the rapid detection of mercapto biomolecules both in vitro and in live cell/tissue/in vivo imaging.

Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels

Lab on a Chip ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 4064-4070 ◽  
Author(s):  
Haiwei Lu ◽  
Kirk Mutafopulos ◽  
John A. Heyman ◽  
Pascal Spink ◽  
Liang Shen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Acoustic Waves ◽  
High Efficiency ◽  
Surface Acoustic Waves ◽  
Microfluidic Channels ◽  
Wide Range

We introduce a microfluidic device that uses traveling surface acoustic waves to lyse bacteria with high efficiency. This lysis method should be applicable to a wide range of bacteria species and can be modified to analyze individual bacteria cells.

scholarly journals Controlling cell–cell interactions using surface acoustic waves

2014 ◽  
Vol 112 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Feng Guo ◽  
Peng Li ◽  
Jarrod B. French ◽  
Zhangming Mao ◽  
Hong Zhao ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Acoustic Waves ◽  
Cancer Metastasis ◽  
Surface Acoustic Waves ◽  
Spatial Arrangement ◽  
Gap Junctional Intercellular Communication ◽  
Wave Technique ◽  
Immunological Reactions ◽  
Gap Junctional

The interactions between pairs of cells and within multicellular assemblies are critical to many biological processes such as intercellular communication, tissue and organ formation, immunological reactions, and cancer metastasis. The ability to precisely control the position of cells relative to one another and within larger cellular assemblies will enable the investigation and characterization of phenomena not currently accessible by conventional in vitro methods. We present a versatile surface acoustic wave technique that is capable of controlling the intercellular distance and spatial arrangement of cells with micrometer level resolution. This technique is, to our knowledge, among the first of its kind to marry high precision and high throughput into a single extremely versatile and wholly biocompatible technology. We demonstrated the capabilities of the system to precisely control intercellular distance, assemble cells with defined geometries, maintain cellular assemblies in suspension, and translate these suspended assemblies to adherent states, all in a contactless, biocompatible manner. As an example of the power of this system, this technology was used to quantitatively investigate the gap junctional intercellular communication in several homotypic and heterotypic populations by visualizing the transfer of fluorescent dye between cells.

Full Band-Gap Silicon Phononic Crystals for Surface Acoustic Waves

2006 ◽  
Author(s):  
Saeed Mohammadi ◽  
Abdelkrim Khelif ◽  
Ryan Westafer ◽  
Eric Massey ◽  
William D. Hunt ◽  
...  
Keyword(s):  
Band Gap ◽  
Acoustic Waves ◽  
Phononic Crystal ◽  
Surface Acoustic Waves ◽  
Hexagonal Lattice ◽  
Phononic Crystals ◽  
Bulk Wave ◽  
Filling Fraction ◽  
Phononic Band Gap ◽  
Wide Range

Periodic elastic structures, called phononic crystals, show interesting frequency domain characteristics that can greatly influence the performance of acoustic and ultrasonic devices for several applications. Phononic crystals are acoustic counterparts of the extensively-investigated photonic crystals that are made by varying material properties periodically. Here we demonstrate the existence of phononic band-gaps for surface acoustic waves (SAWs) in a half-space of two dimensional phononic crystals consisting of hexagonal (honeycomb) arrangement of air cylinders in a crystalline Silicon background with low filling fraction. A theoretical calculation of band structure for bulk wave using finite element method is also achieved and shows that there is no complete phononic band gap in the case of the low filling fraction. Fabrication of the holes in Silicon is done by optical lithography and deep Silicon dry etching. In the experimental characterization, we have used slanted finger interdigitated transducers deposited on a thin layer of Zinc oxide (sputtered on top of the phononic crystal structure to excite elastic surface waves in Silicon) to cover a wide range of frequencies. We believe this to be the first reported demonstration of phononic band-gap for SAWs in a hexagonal lattice phononic crystal at such a high frequency.

scholarly journals Interfacing Live Cells with Surfaces: A Concurrent Control Technique for Quantifying Surface Ligand Activity

2021 ◽  
Author(s):  
Michael C. Robitaille ◽  
Joseph A. Christodoulides ◽  
Patrick Calhoun ◽  
Jeff M. Byers ◽  
Marc P. Raphael
Keyword(s):  
Cellular Adhesion ◽  
Live Cell ◽  
Control Technique ◽  
Live Cells ◽  
Chip Surface ◽  
Concurrent Control ◽  
Activity Measurements ◽  
Surface Ligand ◽  
Ligand Activity

AbstractSurface ligand activity is a key design parameter for successfully interfacing surfaces with cells - whether in the context of in vitro investigations for understanding cellular signaling pathways or more applied applications in drug delivery and medical implants. Unlike other crucial surface parameters, such as stiffness and roughness, surface ligand activity currently lacks a standardized measurement approach that can be readily paired with live cell investigations. To fill this void, we have developed a concurrent control technique for characterizing in vitro ligand surface activity. Pairs of gold-coated glass chips were biofunctionalized with RGD ligand in a parallel workflow: one chip for in vitro applications and the other for surface plasmon resonance (SPR) based RGD activity characterization. Recombinant αVβ3 integrins were injected over the SPR chip surface as mimics of the cellular membrane bound receptors and the resulting binding kinetics parameterized to quantify ligand activity. These activity measurements were correlated with cell morphological features, measured by interfacing MDA-MB-231 cells with the in vitro chip surfaces on the live cell microscope. We show that the SPR concurrent control approach has multiple advantages based on the facts that SPR is a standardized technique and has the sensitivity to measure ligand activity across the most relevant range of extracellular surface densities. Furthermore, by pairing both SPR and in vitro approaches, a comparison of the results can provide biological insights into the nature of cellular adhesion and dynamics.

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