scholarly journals Development of Aqueous Two-Phase Systems Based on Deep Eutectic Solvents for Continuous Protein Extraction in a Microextractor

2021 ◽  
Vol 4 (1) ◽  
pp. 6
Author(s):  
Anabela Ljubić ◽  
Anita Šalić ◽  
Bruno Zelić
Keyword(s):  
Extraction Efficiency ◽  
Protein Extraction ◽  
Batch Reactor ◽  
High Surface ◽  
Deep Eutectic Solvents ◽  
Thermomyces Lanuginosus ◽  
Phase System ◽  
Two Phase ◽  
Promising Alternative ◽  
The Impact

Currently, lipases are one of the most widely used enzymes, especially in catalysis, mostly due to their high activity in mild conditions and wide specificity. Therefore, obtaining the highest possible catalytic activity, which can be achieved through purification, is becoming more and more important. Since most of the purification techniques are time consuming, aqueous two-phase protein extraction is often investigated as a promising alternative. Additionally, this kind of extraction can be carried out in microextractors, which provides not only a continuous processing of raw materials, but also significantly higher efficiencies due to a high surface-to-volume ratio of microchannels. Extraction with deep eutectic solvents (DESs) fulfills all green chemistry principles, because DESs are biodegradable, non-toxic, and recyclable. In this research, the aqueous two-phase system based on natural DES for continuous protein extraction in a microextractor was investigated. The impact of salt concentration on extraction efficiency was investigated in batch experiments with six different previously characterized DESs. After determination of the optimal two-phase system features, the process was transferred to a microextractor. In addition, the selected DES was tested for recyclability while the developed extraction method was verified using raw lipase produced by Thermomyces lanuginosus solid-state cultivation on hull-less pumpkin oil pomace. The highest protein extraction efficiency achieved in a batch reactor was 94.70% for 30 min, while in a microextractor, the highest extraction efficiency obtained was 98.50% for 30 s. Obviously, the extraction process was significantly intensified by continuous microextraction. Additionally, the DES used in the microextraction experiments was efficiently reused in several extraction cycles.

Comparison of hydrophilic and hydrophobic deep eutectic solvents for pretreatment determination of sulfonamides from aqueous environments

2019 ◽  
Vol 11 (46) ◽  
pp. 5901-5909 ◽  
Author(s):  
Yuxin Ma ◽  
Qi Wang ◽  
Tao Zhu
Keyword(s):  
Water Samples ◽  
Extraction Efficiency ◽  
Deep Eutectic Solvents ◽  
Environmental Water ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Environments ◽  
Extraction Solvents

Two types of deep eutectic solvents were synthesized for an aqueous two-phase system to determine the sulfonamides from environmental water samples. The ChCl-Ph based ATPS showed good extraction efficiency. The method avoids use of a large number of extraction solvents.

Investigation of Droplet Impinging on a Heated Porous Surface Under Various Working Conditions; A Mathematical Modeling

2021 ◽  
pp. 2150015
Author(s):  
Mohammad Azadeh ◽  
Hamidreza Khakrah
Keyword(s):  
Droplet Diameter ◽  
High Surface ◽  
Adaptive Method ◽  
Porous Surface ◽  
Droplet Temperature ◽  
Surface Porosity ◽  
Two Phase ◽  
Air Interface ◽  
Accuracy Of Results ◽  
The Impact

This study numerically investigated the behavior of a Newtonian droplet impacting a heated porous surface. In this regard, a two-phase finite volume code was used for laminar flow. The time adaptive method was applied to enhance the accuracy of results and better convergence of the solving process. Also, the dynamic grid adaptation technique was adopted to predict the liquid-air interface precisely. The results were first validated against experimental data at different Weber numbers. Then the effect of variations in the droplet temperature was investigated on the spreading factor. The obtained results revealed that the rise in droplet temperature led to an increase in the maximum spreading diameter due to the reduction in the effects of viscosity, density, and surface tension. In the next step, the effects of droplet impact on the hydrophilic and superhydrophobic surfaces with the porosities of 20–80% were evaluated. The obtained results revealed that the increase in the surface porosity caused a decrease in the droplet diameter during the impact time. Also, at high surface porosity values, the decline in the contact angle influence on the droplet dynamic behavior was observed.

Model for thermal self-ignition of two-phase system in a batch reactor

2012 ◽  
Vol 61 (6) ◽  
pp. 1049-1056
Author(s):  
E. V. Deyun ◽  
L. V. Kustova ◽  
A. I. Kazakov
Keyword(s):  
Batch Reactor ◽  
Phase System ◽  
Two Phase

scholarly journals Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (UA) from Cynomorium songaricum Rupr.

2017 ◽  
Author(s):  
Xifeng Zhang ◽  
Ji Zhang
Keyword(s):  
Ursolic Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Deep Eutectic Solvents ◽  
Selective Extraction ◽  
Phase System ◽  
Green Solvents ◽  
Active Components ◽  
Two Phase ◽  
Solid Ratio

Deep eutectic solvents (DESs) are new green solvents that have attracted the attention of the scientific community mainly due to their unique properties and special characteristics, which are different from those of traditional solvents.A method based on ultrasonically assisted deep eutectic solvent aqueous two-phase systems( UAE-DES-ATPS) was developed for extracting ursolic acid (UA) from Cynomorium songaricum Rupr. Four different types of choline chloride-based DESs were prepared.Choline chloride-glucose (ChCl-Glu) exhibited good selective extraction ability. An optimum DES-ATPS of 36% (w/w) ChCl-Glu and 25% (w/w) K2HPO4 was considered to be a satisfactory system for extracting UA. Response surface methodology (RSM) method was used to optimize the extraction of UA using UAE-DES-ATPS. The optimum ultrasound-assisted conditions were as follows: solvent to solid ratio of 15:1 (g/g), ultrasound power of 470 W, and extraction time of 54 min. Compared with the conventional UAE method, the yields were basically the same, but the presented method had higher purity. The structure of UA did not change between pure UA and UA in the upper phase by UV–vis and FT-IR. This approach using ChCl-based DES-ATPS as a novel extraction system and ultrasound as a source of energy provided better choice for the separation of active components from other natural products.

scholarly journals Biosynthesis of Lactones From Diols Mediated by an Artificial Flavin

2021 ◽  
Author(s):  
Xiaowang Zhang ◽  
Zhuotao Tan ◽  
Chaojian Li ◽  
Siyu Qi ◽  
Mengjiao Xu ◽  
...  
Keyword(s):  
Product Yield ◽  
Product Inhibition ◽  
Phase System ◽  
Regeneration System ◽  
Two Phase ◽  
Promising Alternative ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver ◽  
Valuable Compounds ◽  
Chiral Lactones

Abstract Background: Lactones are important compounds in the field of medicine, material and chemical industry. One of the promising accesses to these flexible scaffolds is NAD(P)+-dependent alcohol dehydrogenases-catalyzed oxidative lactonization of diols, which relies on the construction of an efficient NAD(P)+ regeneration system. Results: In this study, a novel system combining horse liver alcohol dehydrogenase (HLADH) with the synthetic bridged flavin cofactor was established for biosynthesis of lactones. The reaction conditions of this system were optimized and a variety of lactones including chiral lactones were efficiently obtained from various diols. Compared to the previously reported NAD(P)+-regeneration systems, this system showed better regeneration efficiency and product yield. A two-phase system was further applied to solve the problem of product inhibition, and 80% yield was obtained at the condition of 300 mM substrate.Conclusions: This study provides an efficient method to synthesis of lactones from diols under mild conditions. We believe this system will be a promising alternative to promote the synthesis of other valuable compounds.

scholarly journals The interphase distribution of light REE Ce(III) and La(III) in system based on PEG-1500 – NaNO3 – H2O with the quaternary ammonium base addition

2022 ◽  
Vol 1212 (1) ◽  
pp. 012023
Author(s):  
A V Levina ◽  
A Ya Fedorov ◽  
M I Fedorova
Keyword(s):  
Organic Solvents ◽  
Quaternary Ammonium ◽  
Water Solution ◽  
Distribution Coefficients ◽  
Water Soluble ◽  
Process Time ◽  
Phase System ◽  
Two Phase ◽  
Quaternary Ammonium Base ◽  
Promising Alternative

Abstract The problem of e-waste processing and recovery of valuable metals from such waste for the second use is attracting more and more scientists’ attention. Liquid extraction as one of hydrometallurgy steps is a traditional method for the metal recovery. However, application of solvent extraction is not meet the green chemistry principles due to organic solvents. Aqueous two-phase systems based on water-soluble polymers are promising alternative for hazardous organic solvents. In this work the dependencies of Ce(III) and La(III) distribution coefficients from process time and the initial quaternary ammonium base concentration have been achieved. Also, based on the Ce(III) and La(III) extraction isotherms it has been shown that the metals initial concentrations are highly affects the distribution coefficients of studied metals. The possibility of aqueous two-phase system application as a solvent for quaternary ammonium salt for light REE (Ce(III) and La(III)) extraction from water solution has been shown.

scholarly journals The extraction of Zn(II) in aqueous PEG (1550)-(NH4)2SO4 two-phase system using Cl- ions as extracting agent

2007 ◽  
Vol 72 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Aqueous Solutions ◽  
Extraction Efficiency ◽  
Phase Variation ◽  
Chloride Ions ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Experimental Parameters ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

The extraction of Zn(II) in an aqueous PEG (1550) - (NH4)2SO4 two-phase system as a function of several experimental parameters was studied. PEG-based aqueous two-phase systems are composed of two immiscible phases: a polymer-rich phase and a salt-rich phase, which can be used for extraction experiments. In the absence of a suitable extracting agent, for the system consisting of a mixture of equal volumes of 40 mass% PEG and 40 mass% (NH4)2SO4 aqueous solutions, Zn(II) remained predominantly in the salt-rich phase. Variation of the pH of the salt stock solution did not change very much the extraction efficiency. By adding chloride ions, an enhancement of the Zn(II) extraction was observed. The Zn(II) extraction efficiency in presence of Cl- depends on the acidity of the salt stock solution and on the concentration of chloride ions added into the system.

scholarly journals Biosynthesis of lactones from diols mediated by an artificial flavin

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaowang Zhang ◽  
Zhuotao Tan ◽  
Chaojian Li ◽  
Siyu Qi ◽  
Mengjiao Xu ◽  
...  
Keyword(s):  
Product Yield ◽  
Product Inhibition ◽  
Phase System ◽  
Regeneration System ◽  
Two Phase ◽  
Promising Alternative ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver ◽  
Valuable Compounds ◽  
Chiral Lactones

Abstract Background Lactones are important compounds in the field of medicine, material and chemical industry. One of the promising accesses to these flexible scaffolds is NAD(P)+-dependent alcohol dehydrogenases-catalyzed oxidative lactonization of diols, which relies on the construction of an efficient NAD(P)+ regeneration system. Results In this study, a novel system combining horse liver alcohol dehydrogenase (HLADH) with the synthetic bridged flavin cofactor was established for biosynthesis of lactones. The reaction conditions of this system were optimized and a variety of lactones including chiral lactones were efficiently obtained from various diols. Compared to the previously reported NAD(P)+-regeneration systems, this system showed better regeneration efficiency and product yield. A two-phase system was further applied to solve the problem of product inhibition, and 80% yield was obtained at the condition of 300 mM substrate. Conclusions This study provides an efficient method to synthesis of lactones from diols under mild conditions. We believe this system will be a promising alternative to promote the synthesis of other valuable compounds. Graphic abstract

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