scholarly journals Glycosphingolipids Recognized by Acinetobacter baumannii

2020 ◽  
Vol 8 (4) ◽  
pp. 612
Author(s):  
Miralda Madar Johansson ◽  
Mehjar Azzouz ◽  
Beatrice Häggendal ◽  
Karin Säljö ◽  
Henri Malmi ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Bacterial Pathogen ◽  
Basic Element ◽  
Target Tissue ◽  
Binding Assays ◽  
Isolation And Characterization ◽  
Rabbit Small Intestine ◽  
Host Cell Surface ◽  
Hospital Acquired

Acinetobacter baumannii is an opportunistic bacterial pathogen associated with hospital-acquired infections, including pneumonia, meningitis, bacteremia, urinary tract infection, and wound infections. Recognition of host cell surface carbohydrates plays a crucial role in adhesion and enables microbes to colonize different host niches. Here the potential glycosphingolipid receptors of A. baumannii were examined by binding of 35S-labeled bacteria to glycosphingolipids on thin-layer chromatograms. Thereby a selective interaction with two non-acid glycosphingolipids of human and rabbit small intestine was found. The binding-active glycosphingolipids were isolated and, on the basis of mass spectrometry, identified as neolactotetraosylceramide (Galβ4GlcNAcβ3Galβ4Glcβ1Cer) and lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer). Further binding assays using reference glycosphingolipids showed that A. baumannii also bound to lactotriaosylceramide (GlcNAcβ3Galβ4Glcβ1Cer) demonstrating that GlcNAc was the basic element recognized. In addition, the bacteria occasionally bound to galactosylceramide, lactosylceramide with phytosphingosine and/or hydroxy fatty acids, isoglobotriaosylceramide, gangliotriaosylceramide, and gangliotetraosylceramide, in analogy with binding patterns that previously have been described for other bacteria classified as “lactosylceramide-binding”. Finally, by isolation and characterization of glycosphingolipids from human skin, the presence of neolactotetraosylceramide was demonstrated in this A. baumannii target tissue.

scholarly journals Carbapenemase Genes and Multidrug Resistance of Acinetobacter Baumannii: A Cross Sectional Study of Patients with Pneumonia in Southern Vietnam

Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 148 ◽  
Author(s):  
Cuong Hoang Quoc ◽  
Thao Nguyen Thi Phuong ◽  
Hai Nguyen Duc ◽  
Trung Tran Le ◽  
Hang Tran Thi Thu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Demographic Data ◽  
Treatment Options ◽  
Bacterial Pathogen ◽  
Multidrug Resistant ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Carbapenem Resistant ◽  
Hospital Acquired

Background: Acinetobacter baumannii (Ab) is an opportunistic bacterial pathogen found in hospital-acquired infections including nosocomial pneumonia, especially multidrug-resistant Ab. This study aims to survey the drug resistance profiles of Ab isolated from patients in Thong Nhat Dong Nai General Hospital and assess the relationship between genotypes and antibiotic resistance; Methods: Ninety-seven Ab strains isolated from 340 lower respiratory tract specimens among pneumonia patients were used to screen the most common local carbapenemase genes. Antimicrobial susceptibility testing results and demographic data were collected and minimum inhibitory concentrations (MIC) of colistin were also determined; Results: Over 80% and 90% of Ab strains were determined as carbapenem-resistant and multidrug-resistant (MDR), respectively. Most of the strains carried carbapenemase genes, including blaOXA-51, blaOXA-23-like, blaOXA-58-like, and blaNDM-1, with proportions of 97 (100%), 76 (78.4%), 10 (10.3%), 6 (6.2%), respectively. Amongst these genes, blaOXA-23-like was the only gene which significantly influenced the resistance (p < 0.0001); and Conclusions: The severity of Ab antibiotic resistance is urgent and specifically related to carbapenemase encoding genes. Therefore, screening of MDR Ab and carbapenemase for better treatment options is necessary.

scholarly journals Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii

2008 ◽  
Vol 190 (9) ◽  
pp. 3386-3392 ◽  
Author(s):  
Chen Niu ◽  
Katy M. Clemmer ◽  
Robert A. Bonomo ◽  
Philip N. Rather
Keyword(s):  
Acinetobacter Baumannii ◽  
Homoserine Lactone ◽  
Transcriptional Level ◽  
Human Pathogen ◽  
Isolation And Characterization ◽  
Opportunistic Human Pathogen ◽  
Biofilm Development ◽  
Culture Supernatants ◽  
Ethyl Acetate Extracts

ABSTRACT The opportunistic human pathogen Acinetobacter baumannii strain M2 was found to produce distinct acyl-homoserine lactone (AHL) signals based on the use of an Agrobacterium tumefaciens traG-lacZ biosensor. An A. baumannii gene, designated abaI, was cloned and directed AHL production in recombinant Escherichia coli. The AbaI protein was similar to members of the LuxI family of autoinducer synthases and was predicted to be the only autoinducer synthase encoded by A. baumannii. The primary AHL signal directed by AbaI was identified by mass spectrometry as being N-(3-hydroxydodecanoyl)-l-HSL (3-hydroxy-C12-HSL). Minor amounts of at least five additional AHLs were also identified. The expression of abaI at the transcriptional level was activated by ethyl acetate extracts of culture supernatants or by synthetic 3-hydroxy-C12-HSL. An abaI::Km mutant failed to produce any detectable AHL signals and was impaired in biofilm development.

scholarly journals Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium

2016 ◽  
Vol 7 ◽  
Author(s):  
Dinesh M. Fernando ◽  
Izhar U. H. Khan ◽  
Rakesh Patidar ◽  
David R. Lapen ◽  
Guylaine Talbot ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Selective Medium ◽  
Isolation And Characterization

Isolation and characterization of ϕAB2: a novel bacteriophage of Acinetobacter baumannii

2010 ◽  
Vol 161 (4) ◽  
pp. 308-314 ◽  
Author(s):  
Nien-Tsung Lin ◽  
Pei-Yu Chiou ◽  
Kai-Chih Chang ◽  
Li-Kuang Chen ◽  
Meng-Jiun Lai
Keyword(s):  
Acinetobacter Baumannii ◽  
Isolation And Characterization

scholarly journals Comparative Studies on Antibacterial Efficacy of Natural & Artificial Honey on Isolated Pathogens from Burnt Wound Sample

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 269-275
Author(s):  
Vijaya Benerji G ◽  
Anantha Lakshmi Y ◽  
Shankar K ◽  
Punitha V C
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Pathogen ◽  
Isolation And Characterization ◽  
Wound Contamination ◽  
Nutrient Agar Medium ◽  
Pure Cultures ◽  
Made In

The present study dealt with the Isolation and Characterization of a bacterial pathogen from a burnt wound sample. The burnt Wound contamination may be a major muddling previously, patients. Blazed wounds indicated essentially higher bacterial tallies compared for the normal wound. The burnt wound sample was inoculated using nutrient agar medium and medium and incubated at 37oC for 24 hrs. After incubation pure cultures of organisms were made in respective agar mediums. The pure culture of bacteria was identified and characterized as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. These organisms are dangerous pathogens penetrating the wound, producing a lot of pain and exudates. The improvement of blazed wound contamination is not another wonder and is a progressing issue for numerous individuals. Open wounds serve by a passage course to sullying 28 with attacking microorganisms. Furthermore Postponed wound recuperating about contaminated smolder wounds; it will be connected with expanded discomfort, healing center sit tight and human services expenses. The introduce fill in on the honey demonstrated antibacterial movement against the blazed wound pathogenic microscopic organisms for example, such that escherichia coli, staphylococcus aureus Furthermore Pseudomonas aeruginosa. It will meet the requirements of essential products required in humans, animals as well as in the pharmaceutical industry. The natural honey is more effective and safer than artificial honey and the antibiotics against the burnt wound pathogens.

scholarly journals Characterization of Extremely Drug-Resistant and Hypervirulent Acinetobacter baumannii AB030

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 328
Author(s):  
Manu Singh ◽  
P. Malaka De Silva ◽  
Yasser Al-Saadi ◽  
Jacek Switala ◽  
Peter C. Loewen ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Comparative Genomics ◽  
Acinetobacter Baumannii ◽  
Bacterial Pathogen ◽  
Multidrug Resistant ◽  
Mobile Elements ◽  
Drug Resistant

Acinetobacter baumannii is an important nosocomial bacterial pathogen. Multidrug-resistant isolates of A. baumannii are reported worldwide. Some A. baumannii isolates display resistance to nearly all antibiotics, making treatment of infections very challenging. As the need for new and effective antibiotics against A. baumannii becomes increasingly urgent, there is a need to understand the mechanisms of antibiotic resistance and virulence in this organism. In this work, comparative genomics was used to understand the mechanisms of antibiotic resistance and virulence in AB030, an extremely drug-resistant and hypervirulent strain of A. baumannii that is a representative of a recently emerged lineage of A. baumannii International Clone V. In order to characterize AB030, we carried out a genomic and phenotypic comparison with LAC-4, a previously described hyper-resistant and hypervirulent isolate. AB030 contains a number of antibiotic resistance- and virulence-associated genes that are not present in LAC-4. A number of these genes are present on mobile elements. This work shows the importance of characterizing the members of new lineages of A. baumannii in order to determine the development of antibiotic resistance and virulence in this organism.

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