scholarly journals Screening of Lactic Acid Bacteria for the Bio-Control of Botrytis cinerea and the Potential of Lactiplantibacillus plantarum for Eco-Friendly Preservation of Fresh-Cut Kiwifruit

2021 ◽  
Vol 9 (4) ◽  
pp. 773
Author(s):  
Nicola De Simone ◽  
Vittorio Capozzi ◽  
Maria Lucia Valeria de Chiara ◽  
Maria Luisa Amodio ◽  
Samira Brahimi ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Botrytis Cinerea ◽  
Fruits And Vegetables ◽  
Storage Temperature ◽  
Antagonistic Activity ◽  
Climacteric Fruit ◽  
Grey Mold

Botrytis cinerea, responsible for grey mold, represents the first biological cause of fruit and vegetable spoilage phenomena in post-harvest. Kiwifruit is a climacteric fruit particularly prone to this mold infestation during storage. Lactic acid bacteria (LAB) are food-grade bacteria that can synthesize several metabolites with antimicrobial activity and are, therefore, suggested as promising and eco-friendly resources for the bio-control of molds on fruits and vegetables. In this work, we propose the screening of a collection of 300 LAB previously isolated from traditional sourdoughs for their ability to counteract in vitro the growth of Botrytis cinerea CECT 20973. Only 2% of tested LAB strains belonging to Lactiplantibacillus plantarum species, exerted a strong antagonism against B. cinerea. The cell-free supernatants were partially characterized and results clearly indicated that high levels of lactic acid contributed to the antagonistic activity. PAN01 and UFG 121 cell-free supernatants were investigated as potential bio-control agents in a preliminary in vivo assay using freshly cut kiwifruits as a food model. The application of cell-free supernatants allowed to delay the growth of B. cinerea on artificially contaminated kiwifruits until two weeks. The antagonistic activity was greatly affected by the storage temperature (25 °C and 4 °C) selected for the processed fruits, suggesting the importance to include microbial-based solution in a broader framework of hurdle technologies.

scholarly journals First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12437
Author(s):  
Jannette Wen Fang Wu Wu ◽  
Mauricio Redondo-Solano ◽  
Lidieth Uribe ◽  
Rodolfo WingChing-Jones ◽  
Jessie Usaga ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Epithelial Cells ◽  
Lactic Acid Bacteria ◽  
Hela Cells ◽  
High Capacity ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Probiotic Potential

Background Agro-industrial waste from tropical environments could be an important source of lactic acid bacteria (LAB) with probiotic potential. Methods Twelve LAB isolates were isolated from pineapple silages. The species identification was carried out considering 16S rRNA and pheS genes. Experiments to evaluate the probiotic potential of the isolates included survival under simulated gastrointestinal environment, in vitro antagonistic activity (against Salmonella spp. and Listeria monocytogenes), auto-aggregation assays, antibiotic susceptibility, presence of plasmids, adhesiveness to epithelial cells, and antagonistic activity against Salmonella in HeLa cells. Results Lacticaseibacillus paracasei, Lentilactobacillus parafarraginis, Limosilactobacillus fermentum, and Weissella ghanensis were identified. Survival of one of the isolates was 90% or higher after exposure to acidic conditions (pH: 2), six isolates showed at least 61% survival after exposure to bile salts. The three most promising isolates, based on survivability tests, showed a strong antagonistic effect against Salmonella. However, only L. paracasei_6714 showed a strong Listeria inhibition pattern; this isolate showed a good auto-aggregation ability, was resistant to some of the tested antibiotics but was not found to harbor plasmids; it also showed a high capacity for adhesion to epithelial cells and prevented the invasion of Salmonella in HeLa cells. After further in vivo evaluations, L. paracasei_6714 may be considered a probiotic candidate for food industry applications and may have promising performance in acidic products due to its origin.

scholarly journals Evaluation of Lactic Acid Bacteria (LAB) against Shigella sonnei ATCC 25931

2021 ◽  
Author(s):  
María del Pilar Cortés Gaona ◽  
Claudia Amorocho-Cruz
Keyword(s):  
Lactic Acid ◽  
Breast Milk ◽  
Lactic Acid Bacteria ◽  
Organic Acids ◽  
Inhibitory Effect ◽  
Antagonistic Activity ◽  
Shigella Sonnei ◽  
The One

The main functions of Lactic Acid Bacteria (LAB) are the production of organic acids and their characteristic as inhibitors of pathogenic microorganisms. Disease caused by Shigella sonnei is a public health risk factor, as is the resistance they develop to antibiotics. Accordingly, in this study 4 LAB isolated from coffee, quesillo, and breast milk were evaluated under in vitro conditions on Shigella sonnei, considering the presence and cell-free filtration of LAB. The LAB strains were also studied via an antibiotic test using Penicillin, Ceftriaxone, and Ciprofloxacin. The results showed antagonistic activity against Shigella sonnei due to the presence of cells and organic acids. However, the LAB bacteriocins did not inhibit the pathogen. Furthermore, LABs were sensitive to the antibiotics tested. Products from the region such as coffee and quesillo, as well as breast milk are important for their nutritional value and for their LAB content. It would be useful to involve biotechnological research in the coffee and dairy chain on the one hand, and to promote breastfeeding on the other. The tests carried out are qualitative and the inhibitory effect is attributed to the strain and not to a generalization of species. It would also be useful to perform molecular identification of the lactic acid strains studied to introduce them in other food matrices, to evaluate their viability, and to carry out in vivo tests to assess their antimicrobial activity.

scholarly journals Isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens

2016 ◽  
Vol 62 (6) ◽  
pp. 514-524 ◽  
Author(s):  
Sandra Rayén Quilodrán-Vega ◽  
Julio Villena ◽  
José Valdebenito ◽  
María José Salas ◽  
Cristian Parra ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk ◽  
Salmonella Enterica ◽  
Enterohemorrhagic Escherichia Coli ◽  
Salmonella Infection ◽  
Antagonistic Effects ◽  
Gastrointestinal Pathogens

Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bacteria from swine milk have been published. In this work, we isolated several lactic acid bacteria from swine milk and evaluated them for them potential as probiotics. Among the isolated strains, Lactobacillus curvatus TUCO-5E showed antagonistic effects against swine-associated gastrointestinal pathogens. TUCO-5E was able to reduce the growth of enterotoxigenic and enterohemorrhagic Escherichia coli strains as well as pathogenic salmonella. In vitro exclusion and displacement assays in intestinal epithelial cells showed a remarkable antagonistic effect for L. curvatus TUCO-5E against Salmonella sp. strain TUCO-I7 and Salmonella enterica ATCC 13096. Moreover, by using a mouse model of Salmonella infection, we were able to demonstrate that preventative administration of L. curvatus TUCO-5E for 5 consecutive days was capable of decreasing the number of Salmonella enterica serovar Typhimurium in the liver and spleen of treated mice, compared with the controls, and prevented dissemination of the pathogen to the blood stream. Therefore, we have demonstrated here that swine milk is an interesting source of beneficial bacteria. In addition, the results of this work suggest that L. curvatus TUCO-5E is a good candidate to study in vivo the protective effect of probiotics against intestinal infection and damage induced by Salmonella infection in the porcine host.

In vivo and In vitro Mitigation Effects of Lactic Acid Bacteria Derived from Fresh Vegetables on Aflatoxins

2021 ◽  
Vol 62 (5) ◽  
pp. 148-156
Author(s):  
Kenji Oonaka ◽  
Naoki Kobayashi ◽  
Yosuke Uchiyama ◽  
Mioko Honda ◽  
Shiro Miyake ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fresh Vegetables

In vitro and in vivo evaluation of lactic acid bacteria of aquatic origin as probiotics for turbot (Scophthalmus maximus L.) farming

2014 ◽  
Vol 41 (2) ◽  
pp. 570-580 ◽  
Author(s):  
Estefanía Muñoz-Atienza ◽  
Carlos Araújo ◽  
Susana Magadán ◽  
Pablo E. Hernández ◽  
Carmen Herranz ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Scophthalmus Maximus ◽  
In Vivo Evaluation ◽  
Turbot Scophthalmus Maximus

scholarly journals Effect of Lactic Acid Bacteria on the Pharmacokinetics and Metabolism of Ginsenosides in Mice

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1496
Author(s):  
Ji-Hyeon Jeon ◽  
Jaehyeok Lee ◽  
Jin-Hyang Park ◽  
Chul-Haeng Lee ◽  
Min-Koo Choi ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Formation Rate ◽  
Plasma Concentrations ◽  
Control Level ◽  
In Vitro Fermentation ◽  
Fecal Recovery ◽  
Fermentation Test

This study aims to investigate the effect of lactic acid bacteria (LAB) on in vitro and in vivo metabolism and the pharmacokinetics of ginsenosides in mice. When the in vitro fermentation test of RGE with LAB was carried out, protopanaxadiol (PPD) and protopanaxadiol (PPD), which are final metabolites of ginsenosides but not contained in RGE, were greatly increased. Compound K (CK), ginsenoside Rh1 (GRh1), and GRg3 also increased by about 30%. Other ginsenosides with a sugar number of more than 2 showed a gradual decrease by fermentation with LAB for 7 days, suggesting the involvement of LAB in the deglycosylation of ginsenosides. Incubation of single ginsenoside with LAB produced GRg3, CK, and PPD with the highest formation rate and GRd, GRh2, and GF with the lower rate among PPD-type ginsenosides. Among PPT-type ginsenosides, GRh1 and PPT had the highest formation rate. The amoxicillin pretreatment (20 mg/kg/day, twice a day for 3 days) resulted in a significant decrease in the fecal recovery of CK, PPD, and PPT through the blockade of deglycosylation of ginsenosides after single oral administrations of RGE (2 g/kg) in mice. The plasma concentrations of CK, PPD, and PPT were not detectable without change in GRb1, GRb2, and GRc in this group. LAB supplementation (1 billion CFU/2 g/kg/day for 1 week) after the amoxicillin treatment in mice restored the ginsenoside metabolism and the plasma concentrations of ginsenosides to the control level. In conclusion, the alterations in the gut microbiota environment could change the ginsenoside metabolism and plasma concentrations of ginsenosides. Therefore, the supplementation of LAB with oral administrations of RGE would help increase plasma concentrations of deglycosylated ginsenosides such as CK, PPD, and PPT.

scholarly journals A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays

2017 ◽  
Vol 4 ◽  
pp. 83-91 ◽  
Author(s):  
Pamela Mancha-Agresti ◽  
Mariana Martins Drumond ◽  
Fillipe Luiz Rosa do Carmo ◽  
Monica Morais Santos ◽  
Janete Soares Coelho dos Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Dna Delivery ◽  
Eukaryotic Cells ◽  
In Vivo Assays

scholarly journals Transfer of Antibiotic Resistance Marker Genes between Lactic Acid Bacteria in Model Rumen and Plant Environments

2009 ◽  
Vol 75 (10) ◽  
pp. 3146-3152 ◽  
Author(s):  
Niamh Toomey ◽  
�ine Monaghan ◽  
S�amus Fanning ◽  
Declan Bolton
Keyword(s):  
Antibiotic Resistance ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Marker Genes ◽  
Natural Environments ◽  
In Vivo Models ◽  
Filter Mating ◽  
Mating Method

ABSTRACT Three wild-type dairy isolates of lactic acid bacteria (LAB) and one Lactococcus lactis control strain were analyzed for their ability to transfer antibiotic resistance determinants (plasmid or transposon located) to two LAB recipients using both in vitro methods and in vivo models. In vitro transfer experiments were carried out with the donors and recipients using the filter mating method. In vivo mating examined transfer in two natural environments, a rumen model and an alfalfa sprout model. All transconjugants were confirmed by Etest, PCR, pulsed-field gel electrophoresis, and Southern blotting. The in vitro filter mating method demonstrated high transfer frequencies between all LAB pairs, ranging from 1.8 � 10−5 to 2.2 � 10−2 transconjugants per recipient. Transconjugants were detected in the rumen model for all mating pairs tested; however, the frequencies of transfer were low and inconsistent over 48 h (ranging from 1.0 � 10−9 to 8.0 � 10−6 transconjugants per recipient). The plant model provided an environment that appeared to promote comparatively higher transfer frequencies between all LAB pairs tested over the 9-day period (transfer frequencies ranged from 4.7 � 10−4 to 3.9 � 10−1 transconjugants per recipient). In our test models, dairy cultures of LAB can act as a source of mobile genetic elements encoding antibiotic resistance that can spread to other LAB. This observation could have food safety and public health implications.

scholarly journals Actinomycete as biocontrol agents against tomato gray mold disease caused by Botrytis cinerea

2021 ◽  
Vol 48 (3) ◽  
Author(s):  
Hind Lahmyed ◽  
◽  
Rachid Bouharroud ◽  
Redouan Qessaoui ◽  
Abdelhadi Ajerrar ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Extracellular Enzymes ◽  
Chitinase Activity ◽  
Gray Mold ◽  
Antagonistic Activity ◽  
Future Research ◽  
In Planta ◽  
Tomato Fruits

The present work aims to isolate actinomycete bacteria with antagonistic abilities towards Botrytis cinerea, the causal agent of gray mold, from a soil sample collected from the rhizosphere of a healthy tomato grove. In vitro confrontation led to the isolation of 104 actinomycete isolates; fifteen isolates have shown the most significant mortality rate of the mycelial growth of B. cinerea (>50%). Based on the results of this screening, representative strains were selected to verify their in vivo antagonistic activity on tomato fruits; the reduction of B. cinerea has a percentage ranging from 52.38% to 96.19%. Furthermore, the actinomycete isolates were evaluated for their plant growth-promoting (PGP) properties and their ability to produce biocontrol-related extracellular enzymes viz., amylase, protease, cellulase, chitinase, esterases, and lecithinase. Indeed, Ac70 showed high β-1,3-glucanase activity and siderophore production (17U/ml and 43% respectively), and the highest chitinase activity (39μmol/ml) was observed for Ac24. These results indicated that these actinomycetes might potentially control gray mold caused by B. cinerea on tomato fruits. Investigations on enhancing the efficacy and survival of the biocontrol agent in planta and finding out the best formulation are recommended for future research.

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