Evaluation of Antioxidant Activities of Aqueous Extracts and Fractionation of Different Parts of Elsholtzia ciliata

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

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Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Journal of King Saud University - Science ◽

10.1016/j.jksus.2015.04.004 ◽

2015 ◽

Vol 27 (3) ◽

pp. 204-208 ◽

Cited By ~ 12

Author(s):

Vinayak Upadhya ◽

Sandeep R. Pai ◽

Harsha V. Hegde

Keyword(s):

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Total Phenolic ◽

Achyranthes Aspera ◽

Different Parts

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ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF DIFFERENT PARTS OF SUPER RED DRAGON FRUIT (HYLOCEREUS COSTARICENSIS) COLLECTED FROM WEST JAVA-INDONESIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.21571 ◽

2017 ◽

Vol 10 (12) ◽

pp. 290 ◽

Cited By ~ 2

Author(s):

Irda Fidrianny ◽

Nadia Ilham ◽

Rika Hartati

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Total Phenolic ◽

Flavonoid Content ◽

Phytochemical Content ◽

Dragon Fruit ◽

Dpph Method ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

Different Parts

Objectives: The goals of this research were to observe antioxidant properties from different parts of super red dragon fruit (Hylocereus costaricensis) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).Methods: Antioxidant activities were determined using DPPH and ABTS assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method.Results: Inhibitory concentration 50% (IC50) of DPPH scavenging activity of all of the extracts in the range of 2.69 μg/ml was −94.17 μg/ml. The ethyl acetate peel extract of super red dragon fruit expressed the highest TPC (4.56 g GAE/100 g) and the highest total flavonoid content (12.63 g QE/100 g). TPC in flesh extract of super red dragon fruit had a negative and significant correlation with their IC50 of ABTS. The IC50 of DPPH and IC50 of ABTS of flesh extract of super red dragon fruit showed positive and significant correlation.Conclusion: All different parts extracts of super red dragon fruit (except n-hexane flesh extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in flesh extract of super red dragon fruit were the major contributor in antioxidant activities by ABTS method. DPPH and ABTS showed linear results in antioxidant activities of super red dragon fruit flesh extract.

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TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITIES IN CALLUS OF KAEMPFERIA PARVIFLORA

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v9.i6.2021.3992 ◽

2021 ◽

Vol 9 (6) ◽

pp. 77-84

Author(s):

Zuraida Ab Rahman ◽

Ayu Nazreena Othman ◽

Chandradevan A/L Machap ◽

Amirah Balqis Amir Amran ◽

Nur Najwa Arifah Basiron

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Total Content ◽

Total Phenolic ◽

Dichlorophenoxyacetic Acid ◽

Kaempferia Parviflora ◽

Growth Optimization ◽

2,4 Dichlorophenoxyacetic Acid ◽

Different Parts

Black ginger or is one useful medicinal plant that urges the need to be cultivated widely. This plant has some previous studies about their rhizomes and callus growth optimization, but their total content and antioxidant activities are not very . This study aims to study the total content and antioxidant activities of callus of K.. This study is to determine the type and concentration of hormone that give the best effect on the growth of callus in culture from different parts (, and basal part) of K.. Different concentrations were tested with medium 2,4-dichlorophenoxyacetic acid (2,4-D) and acid (NAA) in callus cultivation. The highest total content tested in callus is one with 10.00 mg/L 2,4-D from the part of the plant. The highest antioxidant activity is from the part of the plant with 5.00 mg/L 2,4-D.

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