Microemulsion System for Topical Delivery of Thai Mango Seed Kernel Extract: Development, Physicochemical Characterisation and Ex Vivo Skin Permeation Studies

Background: HQ is used for hyper-pigmentation treatment using conventional creams and gels. These formulations show various disadvantages like poor skin permeation, allergic reactions, and repeated use decreasing patient compliance. Objectives: The present work involved formulation, statistical optimization, and characterization of nanostructured lipid carriers (NLCs) for efficient topical delivery of hydroquinone (HQ) for hyperpigmentation treatment. Methods: The NLCs were optimized exploring Box–Behnken design (BBD) using three independent variables and two dependent variables. Formulation having the minimum size and maximum drug entrapment was considered as optimized formulation. Optimized formulation was evaluated for drug release followed by its freeze-drying. The freeze-dried formulation was subjected to differential scanning calorimetry (DSC) analysis, X-raydiffraction (XRD) analysis, and Fourier transform-infrared spectroscopy (FT-IR) analysis. Furthermore, NLCs based gel was prepared by using Carbopol 934 as a gelling agent. NLCs based gel was evaluated for skin permeation, skin retention, and skin distribution (through confocal microscopic analysis) using pig ear skin. Results: Optimized NLCs showed smaller particle size [(271.9 ± 9) nm], high drug entrapment [(66.4 ± 1.2) %], tolerable polydispersity index (PDI) (0.221 ± 0.012), and zeta potential [(-25.9± 1.2) mV]. The FT-IR analysis revealed excellent compatibility between HQ and other excipients. The Carbopol 934 gel containing NLCs showed high transdermal flux [(163 ± 16.2) μg/cm2/h], permeability coefficient (0.0326 ± 0.0016), and skin permeation enhancement ratio (3.7 ± 0.4) compared to marketed cream of HQ. The results of confocal microscopic (CLSM) analysis revealed the accumulation of optimized NLCs in the lower epidermal layers of skin. Conclusion: NLCs based gel was considered effective in the topical delivery of HQ to treat hyper-pigmentation due high skin permeation, skin retention, and prolonged release of HQ.

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Partial Purification of Polyphenol Oxidase (PPO) from Mango Seed Kernel (MSK) using Alcohol/Salt-based Aqueous Two-Phase System (ATPS)

Current Biotechnology ◽

10.2174/2211550110666210603105630 ◽

2021 ◽

Vol 10 ◽

Author(s):

John Leanard M. Lasquite ◽

Brendaline C. Enopia ◽

Roger Francis E. Senangote ◽

Danilo C. Sabarre Jr. ◽

Camila Flor Yagonia-Lobarbio

Keyword(s):

Polyphenol Oxidase ◽

Alternative Methods ◽

Phase System ◽

Seed Kernel ◽

Mass Ratio ◽

Two Phase ◽

Aqueous Two Phase System ◽

Activity Recovery ◽

Mango Seed Kernel ◽

Mango Seed

Background: Polyphenol oxidase (PPO) has applications in pharmaceutical, environmental, and food industries, however, conventional techniques for its purification are considered costly and laborious. Alternative methods of purification are needed. Methods: This study explored the use of an alcohol/salt-based aqueous two-phase system (ATPS) to partially purify PPO from mango seed kernel (MSK). Specifically, this study determined the effects of 1-propanol/potassium phosphate buffer mass ratio on the partition parameters such as partition coefficients, activity recovery, and purification fold. Results: The enzyme partition coefficient (K E ) was greater than 1, which signified that more PPO was partitioned in the top phase due to its hydrophobic nature. Specifically, the 3.22 1-propanol/PPB mass ratio had the highest K E of 1.91 ± 0.16 and 1.46 ± 0.16 for runs 1 and 2, respectively. Moreover, the same mass ratio led to the highest activity recovery in the top phase reaching up to 73.99 ± 1.56% for run 1 and 64.27 ± 2.48% for run 2. In terms of purification fold, runs 1 and 2 had PF values of 0.55 ± 0.05 and 0.70 ± 0.01, respectively. Since the values obtained were low due to the large number of contaminants extracted together with the target enzyme in the top phase, the use of a back-extraction step recovered the PPO in the bottom phase and resulted in an activity recovery of 82.94 ± 1.05% and a purification fold of 7.01 ± 0.95. Conclusion: Results have shown that varying the mass ratio had significant effects on the partition parameters.

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Characterization of starch from different non-traditional sources and its application as coating in ‘Palmer’ mango fruit

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044011220 ◽

2020 ◽

Vol 44 ◽

Author(s):

Antonio Augusto Marques Rodrigues ◽

Luana Ferreira dos Santos ◽

Rayssa Ribeiro da Costa ◽

Débora Tamara Félix ◽

José Henrique Bernardino Nascimento ◽

...

Keyword(s):

Water Solubility ◽

Amylose Content ◽

Starch Content ◽

Chemical Properties ◽

Seed Kernel ◽

Mango Fruit ◽

Mango Seed Kernel ◽

Randomized Experimental Design ◽

Mango Seed ◽

Biodegradable Coatings

ABSTRACT Yam, cassava, jackfruit seed and mango seed kernel have potential for the extraction and use as starch in the food industry of starch or for the formulation of biodegradable coatings. As a biodegradable coating, starch can be applied in fruits characterized by a fast maturation, such as mango, which requires technologies to increase its shelf life. The aim of this study was to characterize starch from four non-traditional sources and to evaluate their potential as coating for ‘Palmer’ mango fruit. Starches used were extracted from cassava, mango seed kernel, jackfruit seed, and yam, and had their physical, optical, and chemical properties characterized for later use as coatings of ‘Palmer’ mango fruit. Fruits were coated with 3% cassava starch, 3.5% jackfruit seed starch, 3.5% mango seed kernel starch and 3.5% yam starch, and were compared to the control (uncoated). They were then stored at 24.4 ± 0.3 °C and 87 ± 2% RH and evaluated for 12 days. A 5x7 factorial arrangement in a completely randomized experimental design was adopted. Total starch content was higher than 70% in the four sources of starch. Starches from jackfruit and yam had higher amylose content. The four sources of starch had low water solubility and swelling power, with jackfruit seed starch having the highest values. The coating sources were effective in maintaining quality, particularly mango seed kernel starch because it reduced respiratory rate and weight loss in 27.7% and 33.8%, respectively, as well as jackfruit seed starch as it delayed fruit skin yellowing.

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Topical Delivery of Meloxicam using Liposome and Microemulsion Formulation Approaches

Pharmaceutics ◽

10.3390/pharmaceutics12030282 ◽

2020 ◽

Vol 12 (3) ◽

pp. 282 ◽

Cited By ~ 4

Author(s):

Julia Zhang ◽

Anna Froelich ◽

Bozena Michniak-Kohn

Keyword(s):

Transdermal Delivery ◽

Oral Delivery ◽

Ex Vivo ◽

Skin Permeation ◽

Entrapment Efficiency ◽

Topical Delivery ◽

Onset Of Action ◽

Promising Alternative ◽

Inflammatory Drugs ◽

Systemic Onset

The aim of this study is to develop, characterize and compare conventional liposome, deformable liposome (transfersome) and microemulsion formulations as potential topical delivery systems for meloxicam. Liposomes were characterized in terms of vesicle size, zeta potential and entrapment efficiency. For microemulsions, particle size, electrical conductivity and viscosity studies were performed to assess the structure of the investigated systems. An ex vivo skin permeation study has been conducted to compare these formulations. The dermal and transdermal delivery of meloxicam using these formulations can be a promising alternative to conventional oral delivery of non-steroidal anti-inflammatory drugs (NSAIDs) with enhanced local and systemic onset of action and reduced side effects.

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