scholarly journals Study on the Quality Evaluation of Compound Danshen Preparations Based on the xCELLigence Real-Time Cell-Based Assay and Pharmacodynamic Authentication

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2090 ◽  
Author(s):  
Guojun Yan ◽  
Zhitao Zhu ◽  
Liliang Jin ◽  
Jun Chen ◽  
Hui Xie ◽  
...  
Keyword(s):  
Biological Activity ◽  
Real Time ◽  
Quality Evaluation ◽  
Blood Stasis ◽  
Verification Methods ◽  
Dependent Cell ◽  
Response Profiles ◽  
Dose Dependent

Objective: To perform a preliminary study on the quality evaluation of compound Danshen preparations based on the xCELLigence Real-Time Cell-based Assay (RTCA) system and make a pharmacodynamics verification. Methods: The compound Danshen was discussed as a methodological example, and the bioactivity of the compound Danshen preparations were evaluated by real-time cell electronic analysis technology. Meanwhile, an in vivo experiment on an acute blood stasis rat model was performed in order to verify this novel evaluation through the curative effect of dissipating blood stasis. Results: We determined the cell index (CI) and IC50 of the compound Danshen preparations and produced time/dose-dependent cell response profiles (TCRPs). The quality of the three kinds of compound Danshen preparations was evaluated through the RTCA data. The trend of CI and TCRPs reflected the effect of drugs on the cell (promoting or inhibiting), and it was verified that the results correlated with the biological activity of the drugs using a pharmacodynamics experiment. Conclusion: The RTCA system can be used to evaluate the quality of compound Danshen Preparations, and it can provide a new idea and new method for quantitatively characterizing the biological activity of traditional Chinese medicines (TCMs).

Safflower Yellow Prevents Pulmonary Metastasis of Breast Cancer by Inhibiting Tumor Cell Invadopodia

2016 ◽  
Vol 44 (07) ◽  
pp. 1491-1506 ◽  
Author(s):  
Huiying Fu ◽  
Renjie Wu ◽  
Yuanyuan Li ◽  
Lizong Zhang ◽  
Xiaofang Tang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Pulmonary Metastasis ◽  
Breast Cancer Cells ◽  
Dependent Cell ◽  
Response Profiles ◽  
Dose Dependent

Carthamus tinctorius L. is a traditional Chinese medicine that activates blood circulation and dissipates blood stasis, and has been extensively used as antitumor treatment in a clinical setting in single or in compound preparation form. However, empirical evidence and a better understanding of the possible mechanisms involved are still required. Here, we investigated the role of safflower yellow (SY), the active ingredient of C. tinctorius, in the pulmonary metastasis of breast cancer, and the underlying mechanism of action. EGF-meditated time- and dose-dependent cell response profiles were applied to screen for the activity of SY in vitro, while orthotopic lung metastasis and intravenous injection were used to evaluate the antimetastatic role of SY in vivo. SY could dose-dependently inhibit EGF-mediated time- and dose-dependent cell response profiles by inhibiting cytoskeletal rearrangement. We also found that SY significantly inhibited the migration of breast cancer cells in vitro and pulmonary metastasis of breast cancer cells in vivo. Consistent with these phenotypes, formation of invadopodia and the expression of MMP-9 and p-Src proteins were decreased after EGF stimulation in MBA-MD-231 cells treat with SY, as well as in lung metastatic foci. Additionally, circulating tumor cells retained in lung capillaries were also reduced. These results suggest that the antimetastatic effect of SY is due to its inhibition of invadopodia formation, which occurs mainly through Src-dependent cytoskeleton rearrangement. We suggest that SY should be considered as a potential novel therapeutic agent for the treatment of breast cancer.

Enhancement of FSH bioactivity in vivo using site-specific antisera

1997 ◽  
Vol 152 (3) ◽  
pp. 355-363 ◽  
Author(s):  
L Ferasin ◽  
G Gabai ◽  
J Beattie ◽  
G Bono ◽  
A T Holder
Keyword(s):  
Biological Activity ◽  
Treatment Period ◽  
Ovarian Function ◽  
Day Treatment ◽  
Site Specific ◽  
Dose Dependent Fashion ◽  
Snell Dwarf ◽  
Fsh Bioactivity ◽  
Dose Dependent

The ability of site-specific antipeptide antisera to enhance the biological activity of ovine FSH (oFSH) in vivo was investigated using hypopituitary Snell dwarf mice. These animals were shown to respond to increasing doses of oFSH (3·3–90 μg/day), administered in two daily injections over a 5-day treatment period, in a highly significant dose-dependent fashion. The responses measured were increases in uterine weight, ovarian weight and the index of keratinisation in vaginal smears. The dose-dependent response to oFSH confirmed the suitability of this animal model for these investigations and suggested the suboptimal dose of oFSH (20 μg/day) for use in enhancement studies. Five peptides derived from the β subunit of bovine FSH (bFSH) (A, residues 33–47; B, 40–51; C, 69–80; D, 83–94; E, 27–39) were used to generate polyclonal antipeptide antisera. Of these peptides, only A and B produced an antiserum (raised in sheep) capable of recognising 125I-bFSH in a liquid phase RIA. Antisera prepared against peptide A or peptide B were found to significantly enhance the biological activity of 20 μg oFSH/day over a 5-day treatment period. The response to antipeptide antisera alone did not differ significantly from that observed in PBS-injected control animals, neither did the response to FSH alone differ from that observed in animals treated with FSH plus preimmune serum. Thus the enhanced responses are dependent upon the presence of FSH plus antipeptide antiserum. Peptides A and B are located in a region thought to be involved in receptor recognition, this may have implications for the mechanism underlying this phenomenon and/or the structure/function relationships of FSH. That FSH-enhancing antisera can be generated by immunisation of animals with peptides A and B suggests that it may be possible to develop these peptides as vaccines capable of increasing reproductive performance, such as ovulation rate. The high degree of sequence homology between ovine, bovine and porcine (and to a lesser extent human and equine) FSH in the region covered by peptides A and B suggests that these peptides could also be used to promote and regulate ovarian function in all of these species. Journal of Endocrinology (1997) 152, 355–363

Modulation of the biological activity of thyrotrophin by anti-human thyrotrophin monoclonal antibodies

1990 ◽  
Vol 126 (2) ◽  
pp. 333-340 ◽  
Author(s):  
S. R. Page ◽  
A. H. Taylor ◽  
W. Driscoll ◽  
M. Baines ◽  
R. Thorpe ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Biological Activity ◽  
Monoclonal Antibodies ◽  
Cyclic Amp ◽  
Receptor Activation ◽  
Camp Production ◽  
Dose Dependent ◽  
Bovine Tsh

ABSTRACT The mechanism by which monoclonal antibodies enhance the biological activity of a number of hormones is poorly understood. One such antibody (GC73), which binds to human but not bovine TSH, enhances the bioactivity of human TSH in vivo. We have investigated whether GC73 enhancement of TSH bioactivity involves potentiation of hormone-receptor activation assessed by the cyclic AMP (cAMP) responses of both primary human thyrocyte cultures and a TSH-responsive human thyrocyte cell line (SGHTL-45). GC73 had no effect on basal cAMP production. In contrast to its enhancement of the bioactivity of human TSH in vivo, it markedly inhibited the cAMP response to 1 and 10 mU human TSH/ml in primary thyrocytes. This effect was dose-dependent with neutralization of the bioactivity of TSH occurring at 2 mg GC73/ml. GC73 had no effect on the bioactivity of bovine TSH. In contrast, a second anti-TSH monoclonal antibody (TC12), which binds to both human and bovine TSH, inhibited the bioactivity of both species of TSH. Similar results were obtained using SGHTL-45 cells, although the peak concentrations of cAMP were lower. We conclude that binding of GC73 to human TSH resulted in inhibition rather than enhancement of the in-vitro biological activity of human TSH. We suggest that GC73 enhancement of human TSH bioactivity seen in vivo does not result from a mechanism involving potentiation of receptor activation by human TSH. Journal of Endocrinology (1990) 126, 333–340

Analysis of Characteristics of In-Line Magnetic Resonance Sensor

2006 ◽  
Vol 321-323 ◽  
pp. 1221-1224 ◽  
Author(s):  
Seong Min Kim ◽  
Michael J. McCarthy
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Real Time ◽  
Quality Evaluation ◽  
Data Acquisition System ◽  
Internal Quality ◽  
Conveyor System ◽  
Resonance Sensor ◽  
Nmr Techniques

This study was performed to show the feasibility of nuclear magnetic resonance (NMR) techniques for quality evaluation of various agricultural and food products. A real-time in-line NMR quality evaluation sensor was designed, constructed and tested. The device consists of an NMR spectrometer coupled to a conveyor system and a data acquisition system. The conveyor was run at speeds ranging from 0 to 300 mm/s. An NMR signal can be detected when a sample is within ±50 mm of the NMR coil center. The response of NMR sensor was tested using several fruits. The results showed a feasibility of an NMR sensor for evaluating internal quality of various fruits.

scholarly journals Cellular Phenotypic Analysis of Macrophage Activation Unveils Kinetic Responses of Agents Targeting Phosphorylation

2016 ◽  
Vol 22 (1) ◽  
pp. 51-57
Author(s):  
Qian Cao ◽  
Junlin Yao ◽  
Heyuan Li ◽  
Bo Tao ◽  
Yibo Cai ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Macrophage Activation ◽  
Protein Tyrosine Kinase ◽  
Interferon Γ ◽  
Host Immune System ◽  
Phenotypic Analysis ◽  
Dependent Cell ◽  
Pathogen Clearance ◽  
Dose Dependent

Macrophages are highly plastic cells, which serve as sentinels of the host immune system due to their ability to recognize and respond to microbial products rapidly and dynamically. Appropriate regulation of macrophage activation is essential for pathogen clearance or preventing autoimmune diseases. However, regularly used endpoint assays for analyzing macrophage functions have the limitations of being static and non–high throughput. In this study, we introduced a real-time and convenient method based on changes in cellular impedance that are detected by microelectronic biosensors. This new method can record the time/dose-dependent cell response profiles (TCRPs) of macrophages in real time and generates physiologically relevant data. The TCRPs generated from classically interferon-γ/lipopolysaccharide-activated macrophages showed considerable consistency with the data generated from standard endpoint assays. We further explored this approach by using it for global screening of a library of protein tyrosine kinase/phosphatase (PTK/PTP) inhibitors to investigate their impact on macrophage activation. Collectively, our findings suggest that the cellular impedance-based assay provides a promising approach for dynamically monitoring macrophage functions in a convenient and high-throughput manner.

scholarly journals Quality of carcass and meat of large Yorkshire and Swedish landrace pigs

2004 ◽  
Vol 20 (1-2) ◽  
pp. 67-73 ◽  
Author(s):  
Natalija Dzinic ◽  
Ljiljana Petrovic ◽  
Vladimir Tomovic ◽  
Danica Manojlovic ◽  
Svetozar Timanovic ◽  
...  
Keyword(s):  
Meat Quality ◽  
Quality Evaluation ◽  
Selection Model ◽  
Meat Yield ◽  
Pure Breed ◽  
Technological Characteristics ◽  
Selection Of ◽  
The Eu

The comparative quality evaluation of sides and meat was performed after several years of selection of Large Yorkshire (LY) and Swedish Landrace (SL) pigs. The quality of carcass sides e.g. meat yield in sides, was determined in-vivo, using the PIGLOG 105, on the slaughter-line according to Regulation and using the FOM device on a number of pig carcass sides of LY and SL breed(n: LY = 48; SL = 39), and after cooling, partial dissection of left sides was applied according to procedure recommended in the EU, on a smaller number of pure breed sides (n: LY = 18; SL = 17). The investigated selection model in pure breed animals yielded rather good results regarding halves quality of both races (% of meat determined by partial dissection: LY = 57,9; SL = 57,4), while the average meat quality (M. semimembranosus) on the basis of technological characteristics (pHi, pHu, WHCu and colouru) of both investigated races was somewhat poorer and corresponded to RSE quality.

scholarly journals Detailed imaging of mitochondrial transport and precise manipulation of mitochondrial function with genetically-encoded photosensitizers in adult Drosophila neurons

2021 ◽  
Author(s):  
Francesca Mattedi ◽  
George Chennell ◽  
Alessio Vagnoni
Keyword(s):  
Real Time ◽  
Mitochondrial Function ◽  
Super Resolution ◽  
Neuronal Homeostasis ◽  
Regulation Of Transport ◽  
Detailed Imaging ◽  
Precise Distribution ◽  
Adult Drosophila

Abstract Precise distribution of mitochondria is essential for maintaining neuronal homeostasis. Although detailed mechanisms governing the transport of mitochondria have emerged, it is still poorly understood how the regulation of transport is coordinated in space and time within the physiological context of an organism. How alteration in mitochondrial functionality may trigger changes in organellar dynamics also remains unclear in this context. Therefore, the use of genetically-encoded tools to perturb mitochondrial functionality in real time would be desirable. Here we describe methods to interfere with mitochondrial function with high spatiotemporal precision with the use of photosensitisers in vivo in the intact wing nerve of adult Drosophila. We also provide details on how to visualise the transport of mitochondria and to improve the quality of the imaging to attain super-resolution in this tissue.

Organic semiconducting nanoprobe with redox-activatable NIR-II fluorescence forin vivoreal-time monitoring of drug toxicity

2019 ◽  
Vol 55 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Yufu Tang ◽  
Yuanyuan Li ◽  
Zhen Wang ◽  
Feng Pei ◽  
Xiaoming Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Drug Toxicity ◽  
Drug Dose ◽  
Non Invasive ◽  
Dose Dependent

A nitric-oxide-activatable organic semiconducting nanoprobe was developed forin vivo,in situ, real-time and non-invasive NIR-II fluorescence monitoring of drug-dose-dependent hepatotoxicity.

scholarly journals Effect of Alcoholic Extracts of Cymbopogon citratus upon the Control of Colletotrichum gloeosporioides in vitro and upon the Post-harvest Quality of Guavas

2019 ◽  
pp. 1-8
Author(s):  
Ana P. F. A. Santos ◽  
Amanda P. Mattos ◽  
Adriana T. Itako ◽  
João B. Tolentino Júnior ◽  
Gabriela S. Moura ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Soluble Solids ◽  
Post Harvest ◽  
Methanolic Extracts ◽  
Dose Dependent

Aims: This work aimed at evaluating the effects of ethanolic and methanolic extracts of lemongrass upon the control in vitro of Colletotrichum gloeosporioides and upon the post-harvest quality of guavas “Paluma”. Methodology: We analyzed the inhibition of mycelial growth and sporulation of the pathogen at different concentrations of the extracts (8%; 5%; 3%; 1.5% and 0.5%). In the post-harvest assay, the guavas were treated by immersion in distilled water, ethanolic and methanolic extracts (1%; 0.5% and 0.25%) and stored at 25ºC ± 2ºC for eight days. We evaluated mass loss, total soluble solids, total titratable acidity, ratio, reducing and non-reducing sugars, ascorbic acid and pH and the incidence of anthracnose. Results: In the test in vitro, the pathogen growth inhibition was dose-dependent and the sporulation was completely inhibited upon higher concentrations of extract. At post-harvest, the fruits maintained their physicochemical characteristics, and the treatments were not efficient at retarding fruit ripening. Although the tested treatments inhibited the plant pathogen C. gloesporioides in vitro, they were not efficient at controlling the disease in vivo. Conclusion: The extracts showed control in vitro of C. gloeosporioides at 8%. However, the extracts were not effective at controlling the disease after harvest.

Nonxenobiotic manipulation and sulfur precursor specificity of human endothelial cell glutathione

1991 ◽  
Vol 70 (3) ◽  
pp. 1220-1227 ◽  
Author(s):  
I. A. Cotgreave ◽  
D. Constantin-Teodosiu ◽  
P. Moldeus
Keyword(s):  
Morphological Changes ◽  
Umbilical Vein ◽  
Buthionine Sulfoximine ◽  
Complete Medium ◽  
Free Medium ◽  
Sigmoidal Kinetics ◽  
Dependent Cell ◽  
Fresh Plasma ◽  
Dose Dependent

Confluent human umbilical vein endothelial (HUVE) cells were readily (within 1 h) depleted of their glutathione (GSH) by diethylmaleate (0.1-1.0 mM), but dose-dependent cell detachment was noted. Buthionine sulfoximine (BSO, 25 microM) depleted cell GSH with sigmoidal kinetics, showing an initial half-life of depletion of 4-6 h and greater than 95% depletion by 48 h without morphological changes to the cells. However, BSO-dependent depletion of cell GSH was only partially reversible by cell washing and reincubation with complete medium. Likewise, incubation of the cells in sulfur-free medium depleted cell GSH again without morphological changes to the cells. However, unlike with BSO, these cells readily resynthesized GSH when resupplied with complete medium, fresh plasma, or whole blood, with a characteristic overloading of cell GSH (up to 200%) by 12 h. By use of the sulfur-free medium, it was shown that both cystine and cysteine are effective precursors to GSH synthesis in HUVE cells in culture and that cystine is the most likely precursor in vivo. During cystine-supported resynthesis of GSH, high levels of cysteine accumulated in the cells (up to 10% of total soluble free thiol). Physiologically relevant concentrations of extracellular GSH were not as effective as cystine or cysteine in stimulating GSH biosynthesis, whereas nonphysiologically high (mM) concentrations resulted in substantial elevation of GSH levels above those of control cells in a BSO-insensitive manner. These findings provide a simple methodology for the manipulation of HUVE cell GSH in studies of endothelial-specific oxidant toxicity and the sulfur dependence of the biochemistry and turnover of GSH in these human cells.

Sign in / Sign up

Export Citation Format

Share Document