scholarly journals Optimization of Ultrasound-Assisted Extraction Conditions Followed by Solid Phase Extraction Fractionation from Orthosiphon stamineus Benth (Lamiace) Leaves for Antiproliferative Effect on Prostate Cancer Cells

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4183 ◽  
Author(s):  
Siti Hasyimah Suhaimi ◽  
Rosnani Hasham ◽  
Mohamad Khairul Hafiz Idris ◽  
Hassan Fahmi Ismail ◽  
Nor Hazwani Mohd Ariffin ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Solid Phase Extraction ◽  
Acid Content ◽  
Rosmarinic Acid ◽  
Solid Phase ◽  
Harmful Effect ◽  
Maximum Yield ◽  
Extraction Time ◽  
Phase Extraction ◽  
Assisted Extraction

Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of Orthospihon stamineus was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surface methodology (RSM) was performed to develop an extraction method with maximum yield and high rosmarinic acid content. The optimal UAE conditions were as follows: extraction time 21 min, ultrasound amplitudes 62 A, and solvent composition 70% ethanol in water. The crude extract was further fractionated using solid-phase extraction (SPE), where six sequential fractions that varied in polarity (0–100% Acetonitrile in water) were obtained. Next, the six fractions were evaluated for their antioxidant and anti-cancer properties. This study found that Fraction 2 (F2) contained the highest rosmarinic acid content and showed the strongest antioxidant activity. Additionally, F2 showed an anti-proliferative effect against prostate cancer (DU145) with no harmful effect on normal cells.

scholarly journals Simultaneous Determination of 23 Mycotoxins in Broiler Tissues by Solid Phase Extraction UHPLC-Q/Orbitrap High Resolution Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 236
Author(s):  
Youyou Yang ◽  
Zhuolin He ◽  
Lei Mu ◽  
Yunfeng Xie ◽  
Liang Wang
Keyword(s):  
Mass Spectrometry ◽  
Solid Phase Extraction ◽  
Simultaneous Determination ◽  
Solid Phase ◽  
Harmful Effect ◽  
Phase Extraction ◽  
External Standard Method ◽  
Relative Standard ◽  
Limit Of Quantitation

Mycotoxins are a type of toxins harmful for not only animal but also human health. Cooccurrence of multi-mycotoxins could occur for food infected by several molds, producing multi-mycotoxins. It is necessary to develop corresponding determination methods, among which current mass spectrometry (MS) dominates. Currently, the accurate identification and quantitation of mycotoxins in complex matrices by MS with low resolution is still a challenge since false-positive results are typically obtained. Here, a method for the simultaneous determination of 23 mycotoxins in broiler tissues using ultra-high performance liquid chromatography-quadrupole/orbitrap HRMS was established. After the extraction by acetonitrile-water-formic acid (80:18:2, v/v/v), the purification by multifunctional purification solid phase extraction cartridges and the chromatographic separation on a C18 column, representative mycotoxins were determined by HRMS in full scan/data-dependent MS/MS acquisition mode. The quantitation was based on the external standard method. An MS/MS database of 23 mycotoxins was established to achieve qualitative screening and simultaneous quantification. Mycotoxins had a good linear relationship within a certain concentration range with correlation coefficients (r2) larger than 0.991 as well as the limit of quantitation of 1.80–300 μg/kg. The average recoveries at three different levels of low, medium and high fortification were 61–111% with relative standard deviations less than 13.5%. The method was fast, accurate, and suitable for the precise qualification of multiple mycotoxins in broiler tissues. 15 μg/kg zearalenone (ZEN) was detected in one liver sample among 30 samples from markets including chicken breast meat, liver, and gizzards. The result illustrated that the pollution of ZEN should not be neglected considering its harmful effect on the target organ of liver.

scholarly journals Solid-Phase Extraction for Photometric Determination of Rosmarinic Acid in Lemon Balm (Melissa Officinalis) Extracts

2012 ◽  
Vol 11 (1) ◽  
pp. 62-72 ◽  
Author(s):  
Miroslav Ondrejovič ◽  
Tibor Maliar ◽  
Hana Benkovičová ◽  
Jana Kubincová
Keyword(s):  
Solid Phase Extraction ◽  
Flow Rate ◽  
Rosmarinic Acid ◽  
Mobile Phase ◽  
Solid Phase ◽  
Photometric Determination ◽  
Phase Extraction ◽  
Melissa Officinalis ◽  
Lemon Balm

Solid-Phase Extraction for Photometric Determination of Rosmarinic Acid in Lemon Balm (Melissa Officinalis) Extracts The aim of this study was evaluation of the solid-phase extraction for elimination of interference compounds from lemon balm extracts aimed for photometric determination of rosmarinic acid. In experiments, evaluated conditions were as follows: composition and volume of mobile phase, ratio between volume of sample and mass of stationary phase and flow rate of mobile phase during separation. The results indicated that interfered compounds were eliminated. The lemon balm extracts should be pretreated by adsorption on normal stationary phase (silica gel) in ratio sample volume to silica gel weight 1:1 (v/w) elution by mobile phase - diethyl ether: acetic acid (9:1; v/v) - volume - 40 times of crude extract volume - with flow rate 5 ml/min. After selection of SPE conditions, the method was validated with comparison to HPLC analysis. The results suggest that this method may be useable for determination of rosmarinic acid by photometric measurement based on the complexation of Fe2+ ions with rosmarinic acid.

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