scholarly journals Spectroscopic Behaviour of Two Novel Azobenzene Fluorescent Dyes and Their Polymeric Blends

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1368 ◽  
Author(s):  
Rosita Diana ◽  
Ugo Caruso ◽  
Stefano Piotto ◽  
Simona Concilio ◽  
Rafi Shikler ◽  
...  
Keyword(s):  
Fluorescence Quantum Yield ◽  
Azo Dye ◽  
Fluorescent Dyes ◽  
Spectroscopic Properties ◽  
Thin Layers ◽  
Frontier Orbitals ◽  
Spectroscopic Behaviour ◽  
Good Solubility ◽  
Polymeric Blends ◽  
Excellent Chemical

Two novel symmetrical bis-azobenzene red dyes ending with electron-withdrawing or donor groups were synthesized. Both chromophores display good solubility, excellent chemical, and thermal stability. The two dyes are fluorescent in solution and in the solid-state. The spectroscopic properties of the neat crystalline solids were compared with those of doped blends of different amorphous matrixes. Blends of non-conductive and of emissive and conductive host polymers were formed to evaluate the potential of the azo dyes as pigments and as fluorophores. Both in absorbance and emission, the doped thin layers have CIE coordinates in the spectral region from yellow to red. The fluorescence quantum yield measured for the brightest emissive blend reaches 57%, a remarkable performance for a steadily fluorescent azo dye. A DFT approach was employed to examine the frontier orbitals of the two dyes.

Synthesis, spectroscopic properties and DFT calculations of a novel multipolar azo dye and its zinc(II) complex

2017 ◽  
Vol 84 ◽  
pp. 103-108 ◽  
Author(s):  
Barbara Panunzi ◽  
Fabio Borbone ◽  
Amedeo Capobianco ◽  
Simona Concilio ◽  
Rosita Diana ◽  
...  
Keyword(s):  
Dft Calculations ◽  
Azo Dye ◽  
Spectroscopic Properties

Consideration of end effects of DNA hybridization in selection of fluorescent dyes for development of optical biosensors

1999 ◽  
Vol 77 (12) ◽  
pp. 2083-2087 ◽  
Author(s):  
S C Jakeway ◽  
U J Krull
Keyword(s):  
Quantum Efficiency ◽  
Dna Hybridization ◽  
Fluorescent Dyes ◽  
Hydrocarbon Chain ◽  
Spectroscopic Properties ◽  
Excitation Wavelength ◽  
End Effects ◽  
High Quantum Efficiency ◽  
The Stability ◽  
Selection Of

Intercalating fluorescent dyes are in widespread use to detect the presence of double-stranded DNA. Applications include the development of biosensors that rely on the attachment ("tethering") of a dye molecule by a short hydrocarbon chain to the terminus of a strand of DNA so that dye is continuously available and the biosensor is fully reversible. Double strands of DNA have end effects that limit the stability of hybridization and dye intercalation near the termini of the duplexes. Therefore, the selection of the dye must be based on consideration of spectroscopic properties and also issues associated with tether length and the stoichiometry of the binding of the dye with double- and single-stranded DNA. Ethidium bromide (EB) has been used extensively to detect hybridization of DNA in applications such as electrophoresis, gene chips, and biosensors. A number of dyes with greater quantum efficiency than EB for detection of hybridization have been reported. Furthermore, other practical spectroscopic advantages can be gained in terms of improved S/N by use of dyes that have excitation that is red shifted relative to EB. Pyrilium iodide has been disclosed as an intercalator of high quantum efficiency and long excitation wavelength. This work investigates pyrilium iodide in comparison to EB as a candidate for preparation of a tethered dye for detection of hybridization of DNA 20-mers.Key words: biosensors, DNA, hybridization, fluorescence, end effects.

scholarly journals CHARMM-DYES: Parameterization of Fluorescent Dyes for Use with the CHARMM Forcefield

2020 ◽  
Author(s):  
Robert Shaw ◽  
Tristan Johnston-Wood ◽  
Benjamin Ambrose ◽  
Timothy Craggs ◽  
Grant Hill
Keyword(s):  
Quantum Chemical ◽  
Spectroscopic Data ◽  
Fluorescent Dyes ◽  
Dipole Moments ◽  
Complex Structure ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Spectroscopic Properties ◽  
Chemical Calculation ◽  
Dynamics Simulations

<div><div><div><p>We present CHARMM-compatible forcefield parameters for a series of fluorescent dyes from the Alexa, Atto and Cy families, commonly used in F¨orster resonance energy transfer (FRET) experiments. These dyes are routinely used in experiments to resolve the dynamics of proteins and nucleic acids at the nanoscale. However, little is known about the accuracy of the theoretical approximations used in determining the dynamics from the spectroscopic data. Molecular dynamics simulations can provide valuable insights into these dynamics at an atomistic level, but this requires accurate parameters for the dyes. The complex structure of the dyes, and the importance of this in determining their spectroscopic properties, means that parameters generated by analogy to existing parameters do not give meaningful results. Through validation relative to quantum chemical calculation and experiment, the new parameters are shown to significantly outperform those that can be generated automatically, giving better agreement in both the charge distributions and structural properties. These improvements, in particular with regards to orientation of the dipole moments on the dyes, are vital for accurate simulation of FRET processes.</p></div></div></div>

scholarly journals Development of an In Vitro System to Study the Interactions of Aerosolized Drugs with Pulmonary Mucus

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 145 ◽  
Author(s):  
Safar Alqahtani ◽  
Clive J. Roberts ◽  
Snjezana Stolnik ◽  
Cynthia Bosquillon
Keyword(s):  
Drug Disposition ◽  
Fluorescent Dyes ◽  
Thin Layers ◽  
Inhaled Drugs ◽  
Upper Airways ◽  
In Vitro System ◽  
Unique Parameter ◽  
Airway Mucus ◽  
The Impact

Mucus is the first biological component inhaled drugs encounter on their journey towards their pharmacological target in the upper airways. Yet, how mucus may influence drug disposition and efficacy in the lungs has been essentially overlooked. In this study, a simple in vitro system was developed to investigate the factors promoting drug interactions with airway mucus in physiologically relevant conditions. Thin layers of porcine tracheal mucus were prepared in Transwell® inserts and initially, the diffusion of various fluorescent dyes across those layers was monitored over time. A deposition system featuring a MicroSprayer® aerosolizer was optimized to reproducibly deliver liquid aerosols to multiple air-facing layers and then exploited to compare the impact of airway mucus on the transport of inhaled bronchodilators. Both the dyes and drugs tested were distinctly hindered by mucus with high logP compounds being the most affected. The diffusion rate of the bronchodilators across the layers was in the order: ipratropium ≈ glycopyronnium > formoterol > salbutamol > indacaterol, suggesting hydrophobicity plays an important role in their binding to mucus but is not the unique parameter involved. Testing of larger series of compounds would nevertheless be necessary to better understand the interactions of inhaled drugs with airway mucus.

Spectroscopic properties of azo-dye doped PMMA films studied by multi-photon absorption

Optik ◽  
2002 ◽  
Vol 113 (4) ◽  
pp. 189-191 ◽  
Author(s):  
Lisong Yang ◽  
Guiying Wang ◽  
Jiangang Wang ◽  
Guangbin Wang ◽  
Zhizhan Xu
Keyword(s):  
Azo Dye ◽  
Spectroscopic Properties ◽  
Photon Absorption

Synthesis and Spectroscopic Properties of a Novel Bifunctional Pyrene Derivative and its Incorporation into OligoDNA

2015 ◽  
Vol 68 (2) ◽  
pp. 256 ◽  
Author(s):  
Tomohisa Moriguchi ◽  
Satomi Hida ◽  
Fumio Yoneda ◽  
Kazuo Shinozuka
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Fluorescence Spectra ◽  
Bathochromic Shift ◽  
Spectroscopic Properties ◽  
Absorption And Fluorescence Spectra ◽  
Electron Withdrawing Group

A new pyrene derivative bearing a modifiable silyl function (electron-donating group) and cyano function (electron-withdrawing group) was synthesized as a labelling agent for biological substances. The modified pyrene exhibited a marked bathochromic shift in both absorption and fluorescence spectra. The fluorescence quantum yield of the modified pyrene was also significantly increased compared with that of the unmodified pyrene. The modified pyrene was successfully incorporated into oligoDNA.

scholarly journals Substituent and Solvent Polarity on the Spectroscopic Properties in Azo Derivatives of 2-Hydroxynaphthalene and Their Difluoroboranes Complexes

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3387
Author(s):  
Agnieszka Skotnicka ◽  
Przemysław Czeleń
Keyword(s):  
Spectral Properties ◽  
Nmr Spectra ◽  
Fluorescent Dyes ◽  
Photophysical Properties ◽  
Solvent Polarity ◽  
Spectroscopic Properties ◽  
Azo Group ◽  
Group Rotation ◽  
Derivatives Of ◽  
Substituted 1

Novel fluorescent dyes such as difluoroborane complexes of 1-phenylazonaphthalen-2-ol derivatives were successfully synthesized and characterized with a focus on the influence of a substituent and a solvent on the basic photophysical properties. 1H, 11B, 13C, 15N, and 19F nuclear magnetic resonance (NMR) spectra of substituted 1-phenylazonaphthalen-2-ol difluoroboranes and their parent azo dyes were recorded and discussed. The absorption and emission properties of synthesized compounds were investigated in solvents of varying polarity. They were found to be fluorescent despite the presence of the azo group. The azo group rotation was blocked by complexing with -BF2 to get a red shift in absorption. Solvent-dependent spectral properties of compounds were investigated using Lipper-Mataga and Bakhshiev plot. The calculated DFT energies and Frontier Molecular Orbitals calculations of the studied compounds were proved to be consistent with the experimental observations.

scholarly journals AIE/ACQ Effects in Two DR/NIR Emitters: A Structural and DFT Comparative Analysis

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1947 ◽  
Author(s):  
Ugo Caruso ◽  
Barbara Panunzi ◽  
Rosita Diana ◽  
Simona Concilio ◽  
Lucia Sessa ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Spectroscopic Properties ◽  
Time Dependent ◽  
Solid Materials ◽  
Solid Films ◽  
Near Ir ◽  
High Fluorescence ◽  
High Fluorescence Quantum Yield

The effects of aggregation-induced emission (AIE) and of aggregation caused quenching (ACQ) were observed and discussed on two solid materials based on a phenylenevinylene (PV) and a dicyano-PV structure. The brightest emitter in solid films shows a high fluorescence quantum yield in the deep red/near IR (DR/NIR) region (75%). The spectroscopic properties of the two crystalline solids have been described and compared in terms of crystallographic data and time dependent DFT analysis. The influence of the cyano-substituents on AIE/ACQ mechanism activation was discussed.

scholarly journals Solid-State Highly Efficient DR Mono and Poly-Dicyano-Phenylenevinylene Fluorophores

2018 ◽  
Author(s):  
Barbara Panunzi ◽  
Rosita Diana ◽  
Simona Concilio ◽  
Lucia Sessa ◽  
Rafi Shikler ◽  
...  
Keyword(s):  
Solid State ◽  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Spectroscopic Properties ◽  
Crystallographic Data ◽  
Time Dependent ◽  
Aggregation Induced Emission ◽  
Solid Films ◽  
Highly Efficient ◽  
Organic Solid

A highly efficient deep red (DR) emitting organic solid based on a dicyano-phenylenevinylene derivative was reported. The structural and spectroscopic properties of the solid have been described in terms of crystallographic data and time dependent DFT analysis. A noteworthy fluorescence quantum yield of 53% was observed for the brightest emitter cast into solid films. This result can be explained in terms of aggregation-induced emission (AIE) effect.

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