scholarly journals Click Decoration of Bombyx mori Silk Fibroin for Cell Adhesion Control

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4106
Author(s):  
Hidetoshi Teramoto ◽  
Minori Shirakawa ◽  
Yasushi Tamada

Silk fibroin produced by the domesticated silkworm, Bombyx mori, has been studied widely as a substrate for tissue engineering applications because of its mechanical robustness and biocompatibility. However, it is often difficult to precisely tune silk fibroin’s biological properties due to the lack of easy, reliable, and versatile methodologies for decorating it with functional molecules such as those of drugs, polymers, peptides, and enzymes necessary for specific applications. In this study we applied an azido-functionalized silk fibroin, AzidoSilk, produced by a state-of-the-art biotechnology, genetic code expansion, to produce silk fibroin decorated with cell-repellent polyethylene glycol (PEG) chains for controlling the cell adhesion property of silk fibroin film. Azido groups can act as selective handles for chemical reactions such as a strain-promoted azido-alkyne cycloaddition (SPAAC), known as a click chemistry reaction. We found that azido groups in AzidoSilk film were selectively decorated with PEG chains using SPAAC. The PEG-decorated film demonstrated decreased cell adhesion depending on the lengths of the PEG chains. Azido groups in AzidoSilk can be decomposed by UV irradiation. By partially decomposing azido groups in AzidoSilk film in a spatially controlled manner using photomasks, cells could be spatially arranged on the film. These results indicated that SPAAC could be an easy, reliable, and versatile methodology to produce silk fibroin substrates having adequate biological properties.

2016 ◽  
Vol 17 (9) ◽  
pp. 1517 ◽  
Author(s):  
Tetsuo Asakura ◽  
Masanori Endo ◽  
Misaki Hirayama ◽  
Hiroki Arai ◽  
Akihiro Aoki ◽  
...  

RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 65684-65689 ◽  
Author(s):  
Fan Xu ◽  
Meimei Bao ◽  
Longfei Rui ◽  
Jiaojiao Liu ◽  
Jingliang Li ◽  
...  

A self-assembled lipid membrane provides a smooth, hydrophilic and biocompatible surface coating film for materials.


Gels ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 58
Author(s):  
Traian V. Chirila

Fibroin is a fibrous protein that can be conveniently isolated from the silk cocoons produced by the larvae of Bombyx mori silk moth. In its form as a hydrogel, Bombyx mori silk fibroin (BMSF) has been employed in a variety of biomedical applications. When used as substrates for biomaterial-cells constructs in tissue engineering, the oxygen transport characteristics of the BMSF membranes have proved so far to be adequate. However, over the past three decades the BMSF hydrogels have been proposed episodically as materials for the manufacture of contact lenses, an application that depends on substantially elevated oxygen permeability. This review will show that the literature published on the oxygen permeability of BMSF is both limited and controversial. Additionally, there is no evidence that contact lenses made from BMSF have ever reached commercialization. The existing literature is discussed critically, leading to the conclusion that BMSF hydrogels are unsuitable as materials for contact lenses, while also attempting to explain the scarcity of data regarding the oxygen permeability of BMSF. To the author’s knowledge, this review covers all publications related to the topic.


1992 ◽  
Vol 46 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Tetsuo Asakura ◽  
Motohiro Kitaguchi ◽  
Makoto Demura ◽  
Harutoshi Sakai ◽  
Keiichi Komatsu

2013 ◽  
Vol 51 (9) ◽  
pp. 742-748 ◽  
Author(s):  
Mingying Yang ◽  
Wen He ◽  
Yajun Shuai ◽  
Sijia Min ◽  
Liangjun Zhu

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