Pectinase Production from Banana Peel Biomass via the Optimization of the Solid-state Fermentation Conditions of Aspergillus niger Strain

2021 ◽  
Vol 30 (1) ◽  
pp. 257-275
Author(s):  
Nazaitulshila Rasit ◽  
Yong Sin Sze ◽  
Mohd Ali Hassan ◽  
Ooi Chee Kuan ◽  
Sofiah Hamzah ◽  
...  
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Inoculum Size ◽  
Operating Conditions ◽  
Banana Peel ◽  
Fermentation Conditions ◽  
Pectinase Production ◽  
Full Factorial

In this study, the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 °C and 35 °C, moisture content between 40% and 60%, and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35° C of incubation, 60% of moisture content, and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g., pineapple waste, lemon peel, cassava waste, and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al., 2018; Handa et al., 2016; Melnichuk et al., 2020; Thangaratham and Manimegalai, 2014; Salim et al., 2017), thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.

scholarly journals Optimization of Protease from Aspergillus Niger under Solid-State Fermentation Utilizing Shrimp Shell Substrate

2021 ◽  
Vol 11 (6) ◽  
pp. 14809-14824
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Proteolytic Enzymes ◽  
Initial Moisture Content ◽  
Inoculum Size ◽  
Protease Production ◽  
Shrimp Shell

Proteases, also known as proteinases or proteolytic enzymes, belong to a group of hydrolases. It can be applied in numerous fields and industries. Solid-state fermentation (SSF) is recognized as an effective method to produce protease. The ultimate aim of this study is to optimize the production of protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as a solid substrate. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for enzyme production is analyzed using statistical software, Minitab 16. A 23 full factorial experimental design was employed, and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content, and 1.0 inoculum size. It is concluded that SSF protease was successfully produced from Aspergillus niger by utilizing shrimp waste as substrate. Through optimization study, moisture content, the interaction between incubation temperature and moisture content, interaction between moisture content and inoculum size significantly impact protease production.

scholarly journals Lovastatin Production byAspergillus terreusUsing Agro-Biomass as Substrate in Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Yin Wan Ho ◽  
Rosfarizan Mohamad ◽  
Yong Meng Goh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Initial Moisture Content ◽  
Fermentation Time ◽  
Maximum Production ◽  
Additional Nitrogen

Ability of two strains ofAspergillus terreus(ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained usingA. terreusATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM forA. terreusATCC 20542 andA. terreusATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P<0.01) and inoculums size and pH had no significant effect on lovastatin production (P>0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM forA. terreusATCC 20542 and ATCC 74135, respectively, using RS as substrate.

scholarly journals Kinetic Study of Glucosamine Production Using Aspergillus sydowii BCRC 31742 under Solid-State Fermentation

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4832
Author(s):  
Jia Wei Peng ◽  
Ho Shing Wu
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Low Cost ◽  
High Yield ◽  
Aspergillus Sydowii ◽  
Fermentation Conditions ◽  
High Productivity ◽  
State Culture ◽  
Solid State Culture

In the present study, we aimed to obtain a high yield and productivity for glucosamine using a low-cost solid-state culture with Aspergillus sydowii BCRC 31742. The fermentation conditions, such as inoculum biomass, moisture content, and supplemental volume and mineral salt, were chosen to achieve high productivity of glucosamine (GlcN). When the initial supplemental volume used was 3 mL/g substrate, the yield and productivity of GlcN were 48.7 mg/gds and 0.69 mg/gds·h, respectively. This result will be helpful for the industrialization of the process.

Cultural conditions optimization for production of β-galactosidase from Bacillus licheniformis ATCC 12759 under solid-state fermentation

2018 ◽  
Vol 43 (3) ◽  
pp. 240-247 ◽  
Author(s):  
Nurullah Akcan
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Bacillus Licheniformis ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Fermentation Medium ◽  
Inoculum Level ◽  
Cultural Conditions ◽  
Initial Ph ◽  
Fermentation Parameters

AbstractObjective:The aim of this work was to study the optimal cultivation conditions for β-galactosidase production byBacillus licheniformisATCC 12759.Materials and methods:The screening of β-galactosidase production fromB. licheniformisATCC 12759 was performed by solid state fermentation method on media rich with rice bran (RB). Different factors were tested for the optimization of β-galactosidase production.Results:Certain fermentation parameters involving incubation time, incubation temperature, inoculum level, moisture content, initial pH, agitation speed, size of fermentation medium and optimum temperature of β-galactosidase activity were studied separately. Maximal amount of β-galactosidase production was obtained when solid-state fermentation (SSF) was carried out using RB, having inoculum level 35%, moisture content of 20%, initial pH 7.5 at 37°C for 48 h.Conclusion:Results indicated that optimal fermentation conditions play a key role in the maximum production of β-galactosidase fromB. licheniformisATCC 12759. This study shows the potential of the studied enzymes to be promoting candidates for the degradation of lactose and production of important bioproducts.

scholarly journals EFFECT OF FERMENTATION TIME, MOISTURE CONTENT, AND TEMPERATURE ON SORBITOL PRODUCTION VIA SOLID STATE FERMENTATION PROCESS

2017 ◽  
Vol 1 (1) ◽  
pp. 64-71
Author(s):  
Zuriana Sidi Ahmad ◽  
Mimi Sakinah Abdul Munaim
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Product Yield ◽  
High Yield ◽  
Time Range ◽  
Fermentation Time ◽  
Wood Sawdust ◽  
Ssf Process

Malaysia is the largest country that has produced many types of waste. One of it is Meranti wood sawdust. These wastes result in a significant environmental problem if not dispose it in the proper manner. The main objective of this article is to produce the high yield of sorbitol by solid state fermentation (SSF) process from pretreated Meranti wood sawdust using bacterium Lactobacillus plantarum (BAA 793; NCIMB 8826). One factorat a time (OFAT) was studied for further process using solid state fermentation (SSF) process and investigated the effect of relevant parameters (fermentation time, range: 2 hours to 14 hours, moisture content, range: 40% to 90%, temperature, range: 25 oC to 45 oC) to the solid-state fermentation (SSF) process in producing high yield of sorbitol. The highest product yield was obtained at 50% moisture content, at 10 hours of fermentation time and 35 oC of incubation temperature where the concentration of sorbitol was 25.68 g/L respectively. This study also showed that the solid state fermentation (SSF) process will produce the high yield of sorbitol production compared to the submerged fermentation and could serve as a-low cost substrate for bioproducts production especially sorbitol

scholarly journals Enhancement of Laccase Production by Optimizing the Cultural Conditions for Pleurotus Sajor-Caju in Solid-State Fermentation

2021 ◽  
Author(s):  
Shradhdha Sharma ◽  
Duggirala Srinivas Murty
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Cost Effective ◽  
Inoculum Size ◽  
Laccase Production ◽  
Native Page ◽  
Industrial Sectors ◽  
Pleurotus Sajor Caju

Nowadays, a lot of interest has been given to the development of cost-effective and efficient enzyme production technologies. Laccase enzymes are widely used in biotechnological, environmental and industrial sectors. Due to the cost-effectiveness of the solid-state fermentation (SSF) process, it is widely used to produce a broad range of biological products. In this study, optimization of moisture content, temperature, pH, and inoculum size were studied to enhance laccase production ability of Pleurotus sajor-caju in SSF by using One Factor At Time (OFAT) and Response Surface Methodology (RSM). OFAT was used as a baseline study for deducing the experimental design of RSM. The highest production of laccase enzyme (1450 U/g) by Pleurotus sajor-caju on wheat straw was observed at 26°C, 6.0 pH, 72.5 % moisture content, 7.5% inoculum size, 1% fructose and 0.5 % peptone. Unlike the conventional inoculum preparation method, here the inoculum was generated by the spawning method for SSF. The molecular weight of partially purified laccase from Pleurotus sajor-caju was estimated to be around 62 K Da using SDS PAGE. The activity staining of laccase was observed as a zymogram on Native PAGE using ABTS as a substrate. Lignin degradation of wheat straw and its structural disruption due to laccase was observed by Scanning Electron Microscopy (SEM).

scholarly journals Simulation of Lovastatin Production in Solid-State Fermentation via Oil Palm Frond

2021 ◽  
Vol 7 (2) ◽  
pp. 7-10
Author(s):  
N. A. Nata ◽  
F. Mohd Said ◽  
S. Md Shaarani@Md Nawi ◽  
N. Harun
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Oil Palm ◽  
Process Model ◽  
Initial Moisture Content ◽  
Operating Conditions ◽  
Blood Cholesterol ◽  
Oil Palm Frond ◽  
Palm Frond

Lovastatin is a potent drug for lowering the blood cholesterol. It is a competitive inhibitor of 3-hyroxy3-methyl glutaryl coenzyme A (HMG-CoA) reductase, which is a key enzyme in the cholesterol production pathway. Lovastatin increases the good cholesterol or high-density lipoproteins to prevent the formation of plaque inside the blood vessels. This study aims to develop a process model of lovastatin production, produced by Monascus purpureus under solid-state fermentation using oil palm frond. SuperPro Design V9.5 software was used to develop and simulate the process model. Three parameters which are initial moisture content, composition of peptone and potassium, were varied to investigate their effects on lovastatin production. The optimum condition simulated using the process model at pH 7 with 60% initial moisture content, 0.0075 kg/hr of potassium, and 0.0075 kg/hr of peptone was able to produce 0.0288 kg/kg of lovastatin. The simulated results show good agreement with experimental work, with low percentage error of 5.77%, and provide a good approximation on the production of lovastatin under various process operating conditions.

scholarly journals Concurrent production of glycyrrhetic acid 3-O-mono-β-d-glucuronide and lignocellulolytic enzymes by solid-state fermentation of a plant endophytic Chaetomium globosum

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Boliang Gao ◽  
Yiwen Xiao ◽  
Qian Zhang ◽  
Junru Sun ◽  
Zhibing Zhang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Production Efficiency ◽  
Biological Activities ◽  
Inoculum Size ◽  
Chaetomium Globosum ◽  
Glycyrrhetic Acid ◽  
Lignocellulolytic Enzymes ◽  
Fermentation Conditions ◽  
Efficient Strategy

AbstractGlycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG) as an important derivative of glycyrrhizin (GL) shows stronger biological activities and higher sweetness than GL. The biotransformation process is considered as an efficient strategy for GAMG production, due to its mild reaction, high production efficiency and environmentally friendly status. In this study, licorice straw was used for the first time as a medium for GAMG and lignocellulosic enzyme production via solid-state fermentation (SSF) of endophytic fungus Chaetomium globosum DX-THS3. The fermentation conditions including particle size, temperature, seed age, inoculum size, and moisture of substrate were optimized. Furthermore, additional nitrogen sources and carbon sources were screened for GAMG production by C. globosum DX-THS3 of SSF. Under optimal fermentation conditions, the percent conversion of glycyrrhizin reached 90% in 15 days, whereas the control needed 35 days to achieve the same result. The productivity of optimization (P = 2.1 mg/g/day) was 2.33-fold that of non-optimization (P = 0.9 mg/g/day). Meanwhile, high activities of filter paper enzyme (FPase) (245.80 U/g), carboxymethyl cellulase (CMCase) (33.67 U/g), xylanase (83.44 U/g), and β-glucuronidase activity (271.42 U/g) were obtained faster than those in the control during SSF. Our study provides a novel and efficient strategy for GAMG production and indicates C. globosum DX-THS3 as a potential producer of lignocellulolytic enzymes.

scholarly journals Produksi β-Glukosidase Aspergillus niger BIO 2173 dengan Fermentasi Padat Menggunakan Substrat Dedak

2018 ◽  
Vol 8 (01) ◽  
pp. 33
Author(s):  
Sri Sugiwati ◽  
Maggy Thenawidjaja Suhartono ◽  
Muhammad Hanafi ◽  
Hanifah Nuryani Lioe
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Content ◽  
Incubation Period ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Mineral Salts ◽  
Fermentation Conditions ◽  
Fermentation Substrate

Production of β-Glucosidase Aspergillus niger BIO 2173 on Solid State Fermentation Using Rice Bran as SubstrateAbstractβ-Glucosidase (EC 3.2.1.21) is a part of the cellulase enzyme complex which acts synergistically with exoglucanase and endoglucanase to hydrolyze cellulose into glucose. The purpose of this study was to obtain the maximum fermentation conditions for production of b-glucosidase Aspergillus niger BIO 2173 with solid state fermentation using rice bran as fermentation substrate. The factors that affect the production of b-glucosidase which consist of initial pH of the fermentation medium, incubation period, ratio of water content to fermentation substrate, incubation temperature and addition of the Mandel’s mineral salts solution were examined in the study. The results showed that maximum fermentation conditions for β-glucosidase production were at initial of fermentation pH of 2,0, incubation period of 7 days, ratio of water content to substrate of 1:1, and incubation temperature of 32oC. Addition of Mandel’s mineral salts solution to the fermentation substrate at maximum fermentation conditions increased the activity and specific activity of β-glucosidase crude extract up to 5,24 ± 0,57 U/mL and 2,46 ± 0,04 U/mg, respectively.Abstrakβ-Glukosidase (EC 3.2.1.21) merupakan bagian dari enzim multi kompleks selulase, yang bekerja secara sinergis dengan eksoglukanase dan endoglukanase menghidrolisis selulosa menjadi glukosa. Tujuan dari penelitian ini adalah mendapatkan kondisi fermentasi maksimum untuk produksi β-glukosidaseAspergillus niger BIO 2173 dengan fermentasi media padat menggunakan substrat dedak. Pengujian dilakukan terhadap faktor-faktor yang mempengaruhi produksi b-glukosidase, yaitu pH awal medium fermentasi, waktu inkubasi, perbandingan kandungan air terhadap substrat medium fermentasi, suhu inkubasi dan penambahan larutan garam mineral Mandels. Hasil penelitian menunjukkan bahwa kondisi fermentasi maksimum untuk produksi b-glukosidase adalah pada pH awal medium fermentasi 2,0; waktu inkubasi 7 hari, perbandingan kandungan air terhadap substrat medium fermentasi 1:1, dan suhu inkubasi 32oC. Penambahan larutan garam mineral Mandels ke dalam substrat fermentasi pada kondisi fermentasi maksimum menyebabkan peningkatan aktivitas dan aktivitas spesifk ekstrak kasar b-glukosidase masing-masing sebesar 5,24 ± 0,57 U/mL dan 2,46 ± 0,04 U/mg protein. Kata kunci: β-glukosidase, Aspergillus niger, dedak padi, fermentasi padat, ekstrak kasar

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