scholarly journals Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5462
Author(s):  
Anna Ziolkowska ◽  
Bozena Debska ◽  
Magdalena Banach-Szott

The aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and roots) and soil—Albic Brunic Arenosol (horizon A, AE, and Bsv) samples. Phenolic compounds were isolated at two stages by applying acid hydrolysis followed by alkaline re-hydrolysis. The quantitative and qualitative analysis of phenolic compounds was performed with high-performance liquid chromatography with a DAD. The content of phenolic compounds in the extracts depended on the hydrolysis method and it was determined by the type of the research material. The amounts of phenolic compounds contained in the alkaline hydrolysates accounted for 55.7% (soil, horizon Bsv)—454% (roots) of their content in acid hydrolysates. In the extracts from acid hydrolysates, chlorogenic and p-hydroxybenzoic acids were dominant. In the alkaline extracts from the plant material, the highest content was recorded for p-coumaric and ferulic acids, and in the extracts from soil, ferulic and chlorogenic acids. A combination of acid and alkaline hydrolysis ensures the best extraction efficiency of insoluble-bound forms of polyphenols from plant and soil material.

2016 ◽  
Vol 34 (No. 3) ◽  
pp. 254-270
Author(s):  
He Yan-Nan ◽  
Ning Peng-Fei ◽  
Yue Tai-Xin ◽  
Zhang Zhen-Wen

The phenolic compounds of Vitis vinifera cv. Cabernet Gernischet wine in 2010 and 2011 vintage from rain-shelter cultivation and open-field cultivation were detected and compared by high performance liquid chromatography coupled with diode array detector and electrospray ionisation mass spectrometry. The results showed that rain-shelter cultivation increased the total anthocyanin and non-anthocyanin phenolic compound concentration. For the compositions, ratios of peonidin-derivative and malvidin-derivative pigments were similar in the two vintages, rain-shelter cultivation increased the proportion of these derivatives; ratios of delphinidin-derivative and petunidin-derivative pigments were different in the two vintages. In the wine of rain-shelter cultivation, the ratio of flavan-3-ol and hydroxybenzoic acids was increased, the ratio of flavonol was decreased and the composition of other compounds varied between the two vintages. To most of the phenolic compounds identified, their content in wine under rain-shelter cultivation was higher compared to those under open-field cultivation.


2020 ◽  
Vol 16 (1) ◽  
pp. 105-113
Author(s):  
Nara Vanessa dos Anjos Barros ◽  
Bruna Barbosa de Abreu ◽  
Débora Thaís Sampaio da Silva ◽  
Ana Karine de Oliveira Soares ◽  
Maurisrael de Moura Rocha ◽  
...  

Objective: This study aimed to identify and quantify phenolic compounds in the grains of biofortified cowpea (Vigna unguiculata) cultivars before and after cooking. Methods: We analyzed two cultivars of genetically improved cowpeas, namely BRS Aracê and BRS Tumucumaque. Raw and cooked samples of the cultivars were analyzed (in triplicate). The concentrations of phenolics, flavonoids, anthocyanins, and flavanols, as well as the antioxidant activity, were determined. Phenolic compounds were identified and quantified using high-performance liquid chromatography. Results: Grains of cultivar BRS Tumucumaque had higher concentrations of total phenolic compounds before (297.23 ± 4.24 (mean ± standard deviation) mg/100 g) and after (147.15 ± 6.94 mg/100 g) cooking, and higher concentrations of total flavonoids before (49.36 ± 2.02 mg/100 g) and after (23.97± 0.67 mg/100 g) cooking. Anthocyanins were not measured in the grains of either cultivars. There was a statistically significant reduction in the concentration of total flavanols after cooking, with the greater retention in BRS Aracê. Similarly, the antioxidant activity was significantly reduced after cooking, with greater reductions in BRS Tumucumaque than in BRS Aracê. Five phenolic acids were identified and quantified, including gallic, caffeic, and ferulic acids. Conclusion: We conclude that cooking affected the concentrations of phenolic compounds in the cultivars, as well as the antioxidant activity exhibited by these cultivars. The cultivars contained beneficial compounds that can contribute to disease prevention and health maintenance.


2020 ◽  
Vol 16 ◽  
Author(s):  
Natasa P. Kalogiouri ◽  
Natalia Manousi ◽  
Erwin Rosenberg ◽  
George A. Zachariadis ◽  
Victoria F. Samanidou

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results


Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 522
Author(s):  
Luana Beatriz dos Santos Nascimento ◽  
Antonella Gori ◽  
Andrea Raffaelli ◽  
Francesco Ferrini ◽  
Cecilia Brunetti

The use of plant extracts in skin-care cosmetics is a modern trend due to their richness in polyphenols that act as anti-aging molecules. Hibiscus roseus is a perennial species naturalized in Italy, with beautiful soft pink flowers; its phenolic composition and biological activities have not been studied yet. The aim of this study was to characterize and quantify the phenolics and to evaluate the antioxidant, sun protection factor (SPF), and anti-collagenase activities of the ethanolic extracts of H. roseus leaves (HL) and flowers (HF). p-Coumaric, chlorogenic, and trans-ferulic acids derivatives as well as quercetin and kaempferol flavonoids were the main phenolic compounds detected. Catechin, epicatechin, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, kaempferol-7-O-glucoside, tiliroside, oenin, and peonidin-3-O-glucoside were detected only in HF, while phloridzin was exclusive from HL, which also showed greater amounts of hydroxycinnamic acid derivatives. HF was richer in flavonoids and total phenolics, also exhibiting greater antioxidant capacity. The SPF and anti-collagenase activity of both extracts were similar and comparable to those of synthetic standards. The overall results demonstrate that H. roseus extracts are promising sources of bioactive phenolic compounds that could be potentially applied as anti-aging agents in skin-care cosmetics.


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