scholarly journals Synthesis and Characterization of a Mg2+-Selective Probe Based on Benzoyl Hydrazine Derivative and Its Application in Cell Imaging

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2457
Author(s):  
Chunwei Yu ◽  
Yuxiang Ji ◽  
Shaobai Wen ◽  
Jun Zhang
Keyword(s):  
Photoinduced Electron Transfer ◽  
Cell Imaging ◽  
Fluorescence Enhancement ◽  
Transfer Effect ◽  
Ethanol Solution ◽  
Recognition Mechanism ◽  
Hydrazine Derivative ◽  
Fluorescent Response ◽  
Benzoyl Hydrazine

A simple benzoyl hydrazine derivative P was successfully synthesized and characterized as Mg2+-selective fluorescent probe. The binding of P with Mg2+ caused an obvious fluorescence enhancement at 482 nm. The fluorescent, UV-vis spectra, 1H-NMR, and IR spectra confirmed the formation of P-Mg2+ complex, and the formation of a 1:1 stoichiometry complex was proved by Job’s plot and mass spectrometry. The recognition mechanism of P to Mg2+ was owing to the photoinduced electron transfer effect (PET). The fluorescent response was linear in the range of 0.9–4.0 µM with the detection limit of 0.3 µM Mg2+ in water–ethanol solution (1:9, v:v, pH10.0, 20 mM HEPES). In addition, the results of cell imaging of Mg2+ in Hl-7701 cells was satisfying.

scholarly journals Synthesis and characterization of novel protein nanodots as drug delivery carriers with an enhanced biological efficacy of melatonin in breast cancer cells

RSC Advances ◽  
2021 ◽  
Vol 11 (16) ◽  
pp. 9076-9085
Author(s):  
Kanchan Yadav ◽  
Megha Das ◽  
Nurul Hassan ◽  
Archana Mishra ◽  
Jayeeta Lahiri ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Cancer Cells ◽  
Live Cell Imaging ◽  
Biomedical Applications ◽  
Breast Cancer Cells ◽  
Cell Imaging ◽  
Synthesis And Characterization ◽  
Novel Protein

A novel nanodot-using protein has been synthesized for the live cell imaging and drug delivery of melatonin in breast cancer cells. Its unique properties hold potential for various biomedical applications in the field of bioimaging and drug delivery.

scholarly journals Combining Single RNA Sensitive Probes with Subdiffraction-Limited and Live-Cell Imaging Enables the Characterization of Virus Dynamics in Cells

ACS Nano ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. 302-315 ◽  
Author(s):  
Eric Alonas ◽  
Aaron W. Lifland ◽  
Manasa Gudheti ◽  
Daryll Vanover ◽  
Jeenah Jung ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Virus Dynamics ◽  
In Cells

Pillar[5]arene-based supramolecular assemblies with two-step sequential fluorescence enhancement for mitochondria-targeted cell imaging

2020 ◽  
Vol 8 (44) ◽  
pp. 15622-15625
Author(s):  
Hao Guo ◽  
Xin Yan ◽  
Bing Lu ◽  
Jin Wang ◽  
Xiaolei Yuan ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescence Enhancement ◽  
Fluorescence Emission ◽  
Supramolecular Assemblies ◽  
Live Cell ◽  
Supramolecular Nanoparticles

Two-step sequential fluorescence emission enhanced supramolecular nanoparticles are constructed from pillar[5]arene based host–guest interaction and a linear amphiphile. These supramolecular nanoparticles can be applied in mitochondria-targeted live cell imaging.

Synthesis and Characterization of Zinc Coated Carica papaya Extracts for Solid-State Application

2021 ◽  
Vol 885 ◽  
pp. 53-57
Author(s):  
Moses E. Emetere ◽  
Ikechukwu M. Ahiara
Keyword(s):  
Carica Papaya ◽  
Ethanol Solution ◽  
Methanol Solution ◽  
Synthesis And Characterization ◽  
Short And Long Term ◽  
Green Solution ◽  
Conventional Type ◽  
Butanol Solution

The evolution of semiconductor from the conventional type to organic semiconductor has not convincingly shown that it is eco-friendly both in the short and long term. This research presents the green solution bio-semiconductor that was synthesized from zinc coated carica papaya. It was observed that carica papaya extracts do not respond significantly to the extracting solution (methanol, ethanol and butanol). The band gap of sample 1 (extract in methanol solution), sample 2 (extract in ethanol solution) and sample 3 (extract in butanol solution) was calculated as 1.98 eV, 2.01eV and 1.93 eV respectively. Further research is therefore recommended for the perfection of the bio-semiconductor.

Synthesis and characterization of fluorescent perylene bisimide-containing glycopolymers for Escherichia coli conjugation and cell imaging

Polymer ◽  
2011 ◽  
Vol 52 (25) ◽  
pp. 5764-5771 ◽  
Author(s):  
Li Qun Xu ◽  
Chao Huang ◽  
Rong Wang ◽  
Koon-Gee Neoh ◽  
En-Tang Kang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Imaging ◽  
Synthesis And Characterization ◽  
Perylene Bisimide

scholarly journals Thrombin Metz: characterization of the dysfunctional thrombin derived from a variant of human prothrombin

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 927-934
Author(s):  
MJ Rabiet ◽  
M Jandrot-Perrus ◽  
JP Boissel ◽  
J Elion ◽  
F Josso
Keyword(s):  
High Performance ◽  
Fluorescence Enhancement ◽  
Antithrombin Iii ◽  
Factor Xa ◽  
Competitive Inhibitor ◽  
Amidolytic Activity ◽  
Direct Inhibition ◽  
High Affinity ◽  
Factor Va

Thrombin Metz and normal thrombin, resulting from activation of the respective prothrombins by factor Xa in the presence of calcium, phospholipid, and factor Va, were purified by chromatography on sulfopropyl Sephadex. By physicochemical criteria, thrombin Metz is identical to normal thrombin. Its functional properties were investigated in some reactions in which thrombin is classically involved. Thrombin Metz exhibits less than 4% of fibrinogen clotting activity. Both Km and Kcat, determined on S2238, are abnormal. Titration with the high-affinity competitive inhibitor of thrombin, DAPA, shows that fluorescence enhancement of the probe is only 34% in binding to thrombin Metz when compared to that observed in binding to normal thrombin. High-performance liquid chromatography has been used to measure the simultaneous rate of release of fibrinopeptides A and B. A decreased release rate for both fibrinopeptides, more marked for fibrinopeptide B, results in a slow fibrin polymerization, as followed by absorbance at 450 nm. Thrombin Metz is less than 5% as effective as normal thrombin in inducing platelet aggregation. Interaction with antithrombin III is slower than normal when followed by SDS gel electrophoresis and inhibition of the amidolytic activity of thrombin on S2238. This abnormality is not observed in the presence of heparin. However, thrombin Metz binds less tightly to a heparin-Sepharose column, and the direct inhibition of heparin on its activity on S2238 is weaker. From these results, we can predict that the defect in thrombin Metz affects the catalytic site or its vicinity and, jointly or consequently, the region of interaction of thrombin with antithrombin III and heparin.

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