Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley

Arabinoxylan (HBAX-60) was fractioned from alkaline-extracted arabinoxylan (HBAX) in the whole grain of hull-less barley (Hordeum vulgare L. var. nudum Hook. f. Poaceae) by 60% ethanol precipitation, which was studied for physicochemical properties and structure elucidation. Highly purified HBAX-60 mainly composed of arabinose (40.7%) and xylose (59.3%) was created. The methylation and NMR analysis of HBAX-60 indicated that a low-branched β-(1→4)-linked xylan backbone possessed un-substituted (1,4-linked β-Xylp, 36.2%), mono-substituted (β-1,3,4-linked Xylp, 5.9%), and di-substituted (1,2,3,4-linked β-Xylp, 12.1%) xylose units as the main chains, though other residues (α-Araf-(1→, β-Xylp-(1→, α-Araf-(1→3)-α-Araf-(1→ or β-Xylp-(1→3)-α-Araf-(1→) were also determined. Additionally, HBAX-60 exhibited random coil conformation in a 0.1 M NaNO3 solution. This work provides the properties and structural basis of the hull-less barley-derived arabinoxylan, which facilitates further research for exploring the structure–function relationship and application of arabinoxylan from hull-less barley.

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Antioxidant activity of whole grain Qingke (Tibetan Hordeum vulgare L.) toward oxidative stress in d-galactose induced mouse model

Journal of Functional Foods ◽

10.1016/j.jff.2018.04.036 ◽

2018 ◽

Vol 45 ◽

pp. 355-362 ◽

Cited By ~ 4

Author(s):

Xuejuan Xia ◽

Yuxiao Xing ◽

Guannan Li ◽

Jinsong Wu ◽

Jianquan Kan

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Hordeum Vulgare ◽

Mouse Model ◽

Whole Grain ◽

Hordeum Vulgare L

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Germination and microwave processing of barley ( Hordeum vulgare L ) changes the structural and physicochemical properties of β- d -glucan & enhances its antioxidant potential

Carbohydrate Polymers ◽

10.1016/j.carbpol.2016.07.022 ◽

2016 ◽

Vol 153 ◽

pp. 696-702 ◽

Cited By ~ 29

Author(s):

Mudasir Ahmad ◽

Adil Gani ◽

Asima Shah ◽

Asir Gani ◽

F.A. Masoodi

Keyword(s):

Hordeum Vulgare ◽

Physicochemical Properties ◽

Microwave Processing ◽

Antioxidant Potential ◽

Hordeum Vulgare L

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Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.)

PLoS ONE ◽

10.1371/journal.pone.0216978 ◽

2019 ◽

Vol 14 (5) ◽

pp. e0216978 ◽

Cited By ~ 2

Author(s):

Xiangyun Fan ◽

Juan Zhu ◽

Wenbin Dong ◽

Yuandong Sun ◽

Chao Lv ◽

...

Keyword(s):

Hordeum Vulgare ◽

Pasting Properties ◽

Whole Grain ◽

Hordeum Vulgare L ◽

Whole Grain Flour

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IRS-1/PI3K/Akt pathway and miRNAs are involved in whole grain highland barley (Hordeum vulgare L.) ameliorating hyperglycemia of db/db mice

Food & Function ◽

10.1039/d0fo01990a ◽

2020 ◽

Vol 11 (11) ◽

pp. 9535-9546

Author(s):

Na Deng ◽

Ruixue Guo ◽

Bisheng Zheng ◽

Tong Li ◽

Rui Hai Liu

Keyword(s):

Hordeum Vulgare ◽

Molecular Mechanism ◽

Mirna Expression ◽

Akt Pathway ◽

Whole Grain ◽

Hordeum Vulgare L ◽

Highland Barley

The present investigation further unravels the underlying molecular mechanism of WGH on T2DM: IRS-1/PI3K/Akt pathway and related miRNA expression.

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Structure elucidation of an immunostimulatory arabinoxylan-type polysaccharide prepared from young barley leaves (Hordeum vulgare L.)

Carbohydrate Polymers ◽

10.1016/j.carbpol.2016.09.056 ◽

2017 ◽

Vol 157 ◽

pp. 282-293 ◽

Cited By ~ 15

Author(s):

Hoon Kim ◽

Hee-Do Hong ◽

Kwang-Soon Shin

Keyword(s):

Hordeum Vulgare ◽

Structure Elucidation ◽

Hordeum Vulgare L ◽

Barley Leaves

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Physicochemical properties and structural characteristics of whole grain Oryza sativa L. with different treatments

Food Science and Technology International ◽

10.1177/1082013215600078 ◽

2015 ◽

Vol 22 (4) ◽

pp. 333-342 ◽

Cited By ~ 2

Author(s):

Micaela Albarracín ◽

Pau Talens ◽

Nuria Martínez-Navarrete ◽

Rolando J González ◽

Silvina R Drago

Keyword(s):

Oryza Sativa ◽

Physicochemical Properties ◽

Oryza Sativa L ◽

Structural Characteristics ◽

Whole Grain

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Highland Barley Whole Grain (Hordeum vulgare L.) Ameliorates Hyperlipidemia by Modulating Cecal Microbiota, miRNAs, and AMPK Pathways in Leptin Receptor-Deficient db/db Mice

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.0c04780 ◽

2020 ◽

Vol 68 (42) ◽

pp. 11735-11746

Author(s):

Na Deng ◽

Ziqian He ◽

Ruixue Guo ◽

Bisheng Zheng ◽

Tong Li ◽

...

Keyword(s):

Hordeum Vulgare ◽

Leptin Receptor ◽

Whole Grain ◽

Hordeum Vulgare L ◽

Cecal Microbiota ◽

Highland Barley

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Ultrastructure and Senescence in an Achloroplastic Mutant of Hordeum vulgare L. cv. Dyan

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124628 ◽

1992 ◽

Vol 50 (1) ◽

pp. 844-845

Author(s):

R.H.M. Cross ◽

C.E.J. Botha ◽

A.K. Cowan ◽

B.J. Hartley

Keyword(s):

Cell Wall ◽

Hordeum Vulgare ◽

Leaf Tissue ◽

Ultrastructural Changes ◽

Hordeum Vulgare L ◽

Mesophyll Cells ◽

Lethal Mutant ◽

Cytoplasmic Matrix ◽

Close Proximity ◽

Pinocytotic Vesicles

Senescence is an ordered degenerative process leading to death of individual cells, organs and organisms. The detection of a conditional lethal mutant (achloroplastic) of Hordeum vulgare has enabled us to investigate ultrastructural changes occurring in leaf tissue during foliar senescence.Examination of the tonoplast structure in six and 14 day-old mutant tissue revealed a progressive degeneration and disappearance of the membrane, apparently starting by day six in the vicinity of the mitochondria associated with the degenerating proplastid (Fig. 1.) where neither of the plastid membrane leaflets is evident (arrows, Fig. 1.). At this stage there was evidence that the mitochondrial membranes were undergoing retrogressive changes, coupled with disorganization of cristae (Fig. 2.). Proplastids (P) lack definitive prolamellar bodies. The cytoplasmic matrix is largely agranular, with few endoplasmic reticulum (ER) cisternae or polyribosomal aggregates. Interestingly, large numbers of actively-budding dictysomes, associated with pinocytotic vesicles, were observed in close proximity to the plasmalemma of mesophyll cells (Fig. 3.). By day 14 however, mesophyll cells showed almost complete breakdown of subcellular organelle structure (Fig. 4.), and further evidence for the breakdown of the tonoplast. The final stage of senescence is characterized by the solubilization of the cell wall due to expression and activity of polygalacturonase and/or cellulose. The presence of dictyosomes with associated pinocytotic vesicles formed from the mature face, in close proximity to both the plasmalemma and the cell wall, would appear to support the model proposed by Christopherson for the secretion of cellulase. This pathway of synthesis is typical for secretory glycoproteins.

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