Aloe djiboutiensis: Antioxidant Activity, Molecular Networking-Based Approach and In Vivo Toxicity of This Endemic Species in Djibouti

For the first time, the study of the antioxidant activity, the characterization of the phytoconstituants, and the evaluation of in vitro and in vivo toxicity of A. djiboutiensis leave and latex are performed. The antioxidant activity of both latex (ADL) and the methanolic extract of leaves (ADM) is determined using 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) scavenging radical methods and ferric reducing/antioxidant power (FRAP) assay. The phytochemical study of latex is done using Liquid Chromatography-Mass Spectrometry (LC-MS/MS) and a molecular networking-based approach. The evaluation of in vivo toxicity is performed on mice by oral gavage with a suspension of ADL. Our results show that weak antioxidant activity of ADL and ADM in opposition to their high polyphenol, 83.01 mg and 46.4 mg expressed in gallic acid equivalent (GAE)/g of dry weight (DW), respectively, and flavonoid contents 13.12 mg and 4.25 mg expressed in quercetin equivalent (QE)/g dry weight (DW), respectively. Using the Global Natural Products Social Molecular Networking (GNPS) website, nine (9) anthraquinones derivatives, ten (10) chromones derivatives, two (2) flavonols/ chromones isomers are annotated in the molecular network. The treated mice do not display abnormalities in their general physical appearance and biochemistry parameters, compared to the controls. Only glucose and calcium levels are slightly higher in male treated mice compared to the vehicles.

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Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage

Molecules ◽

10.3390/molecules23092216 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2216 ◽

Cited By ~ 7

Author(s):

Cai-Ning Zhao ◽

Guo-Yi Tang ◽

Qing Liu ◽

Xiao-Yu Xu ◽

Shi-Yu Cao ◽

...

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

High Performance ◽

Antioxidant Properties ◽

Dry Weight ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Herbal Tea ◽

Antioxidant Power

The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe2+/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and p-coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.

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Evaluation of Antioxidant and Antibacterial Activities, Cytotoxicity of Acacia seyal Del Bark Extracts and Isolated Compounds

Molecules ◽

10.3390/molecules25102392 ◽

2020 ◽

Vol 25 (10) ◽

pp. 2392 ◽

Cited By ~ 3

Author(s):

Abdirahman Elmi ◽

Rosella Spina ◽

Arnaud Risler ◽

Stéphanie Philippot ◽

Ali Mérito ◽

...

Keyword(s):

Antioxidant Activity ◽

Crude Extract ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Water Extract ◽

Frap Assay ◽

Antioxidant Power ◽

Dpph Method ◽

Acacia Seyal

Water extract of Acacia seyal bark is used traditionally by the population in Djibouti for its anti-infectious activity. The evaluation of in vitro antibacterial, antioxidant activities and cytotoxicity as well as chemical characterization of Acacia seyal bark water and methanolic extracts were presented. The water extract has a toxicity against the MRC-5 cells at 256 μg/mL while the methanolic extract has a weak toxicity at the same concentration. The methanolic extract has a strong antioxidant activity with half maximal inhibitory concentration (IC50) of 150 ± 2.2 μg/mL using 1-diphenyl-2-picrylhydrazyl (DPPH) and IC50 of 27 ± 1.3 μg/mL using 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical methods. For ferric reducing/antioxidant power (FRAP) assay, the result is 45.74 ± 5.96 μg Vitamin C Equivalent (VCE)/g of dry weight (DW). The precipitation of tannins from methanol crude extract decreases the MIC from 64 µg/mL to 32 µg/mL against Staphylococcus aureus and Corynebacterium urealyticum. However, the antioxidant activity is higher before tannins precipitation than after (IC50 = 150 µg/mL for methanolic crude extract and 250 µg/mL after tannins precipitation determined by DPPH method). By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, the results showed that the condensed tannins consist of two types of catechin and gallocatechin-based oligomers. The fractionation led to the identification of three pure compounds: two flavanols catechin and epicatechin; one triterpene as lupeol; and a mixture of three steroids and one fatty acid: campesterol, stigmasterol, clionasterol, and oleamide.

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In- vitro antioxidant and In vivo Anti-inflammatory Potentials of Marantochloa Leucantha (Marantaceae) Extracts and Fractions

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v18i2.44463 ◽

2019 ◽

Vol 18 (2) ◽

pp. 233-240

Author(s):

Wilfred O Obonga ◽

Charles O Nnadi ◽

Chinonye C Chima ◽

Sunday N Okafor ◽

Edwin O Omeje

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Paw Edema ◽

Frap Assay ◽

Rat Paw Edema ◽

Etoac Fraction ◽

High Antioxidant Activity ◽

Anti Inflammatory

This study evaluated the antioxidant and anti-inflammatory properties of Marantochloa leucantha (Marantaceae). The in vitro antioxidant activity of the extracts and solvent fractions was evaluated by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant potential (FRAP) assay models and in vivo anti-inflammatory activity by the rat paw edema model. The phytochemical screening indicated the presence of tannins, terpenoids, steroids, flavonoids, reducing sugar and phenolics. The antioxidant assay showed that all the extracts exhibited high antioxidant activity comparable with ascorbic and gallic acid controls. In DPPH model, a 250 μg/ml EtOAc fraction of the leaves showed antioxidant activity of 93.9 ± 1.7 % (EC50 0.82 μg/ml) and a 1000 μg/ml of same stem fraction produced 91.9 ± 0.3 % activity (EC50 1.38 μg/ml). In the FRAP model, EtOAc fraction exhibited 31.1±0.7 and 92.0 ± 2.2 μM Fe2+/g of dried leaves and stem, respectively at 1000 μg/ml FeSO4 equivalent. The anti-inflammatory potential of the plant showed that the crude stem extract and fractions at 200 - 600 mg/kg exhibited significant (p < 0.01) dose-related inhibition of paw edema in rats. A 200 mg/kg EtOAc fraction showed 18.8 % inhibition compared to 31 % observed in diclofenac-treated rats in 2 h post albumin challenge. These findings validated the folkloric use of this plant in the treatment of diseases associated to the oxidative stress and could further provide promising lead compounds with potent antioxidant and anti-inflammatory activities Dhaka Univ. J. Pharm. Sci. 18(2): 233-240, 2019 (December)

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Ginsenoside and phenolic compounds in hydromethanolic extracts of American ginseng cell cultures and their antioxidant properties

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.3638 ◽

2019 ◽

Vol 88 (4) ◽

Cited By ~ 1

Author(s):

Ewa Kochan ◽

Grażyna Szymańska ◽

Izabela Grzegorczy-Karolak ◽

Piotr Szymczyk ◽

Monika Sienkiewicz

Keyword(s):

Antioxidant Activity ◽

Suspension Culture ◽

Antioxidant Properties ◽

Dry Weight ◽

American Ginseng ◽

Total Phenolic ◽

Panax Quinquefolium ◽

Callus Line ◽

Antioxidant Power

The present study was performed to investigate the antioxidant properties of callus and suspension culture extracts of Panax quinquefolium (American ginseng). The ginsenoside content and the total phenolic content (TPC) in these cultures were also examined. The total amount of the nine studied saponins was found to be 2.08, 1.69, and 0.202 mg g−1 dry weight in red callus line (RCL), green callus line (GCL), and suspension cultures, respectively, by HPLC analysis. The TPC was estimated using the Folin–Ciocalteu method. The TPC of the suspension culture extracts was approximately 36.7% and 17.6% higher than that of the RCL and GCL, respectively. The antioxidant activity of the extracts was evaluated using the in vitro ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] and FRAP (ferric reducing antioxidant power) assays; the methanolic P. quinquefolium suspension culture extracts demonstrated stronger antioxidant activity than that of the callus cultures.

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Phytochemical composition, antifungal activity, in vitro and in vivo toxicity of Syzygium cumini (L.) Skeels leaves extract

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas ◽

10.37360/blacpma.21.20.5.40 ◽

2021 ◽

Vol 20 (5) ◽

pp. 536-555

Author(s):

Ernani Canuto Figueiredo Junior ◽

◽

Yuri Wanderley Cavalcanti ◽

Andressa Brito Lira ◽

Hilzeth de Luna Freire Pessoa ◽

...

Keyword(s):

Antioxidant Activity ◽

Antifungal Activity ◽

Galleria Mellonella ◽

In Vitro Assays ◽

Syzygium Cumini ◽

Candida Spp ◽

In Vivo Toxicity ◽

Phytochemical Composition

This study determined phytochemical composition, antifungal activity and toxicity in vitro and in vivo of Syzygium cumini leaves extract (Sc). Thus, was characterized by gas chromatography coupled to mass spectrometry and submitted to determination of Minimum Inhibitory (MIC) and Fungicidal concentrations (MFC) on reference and clinical strains of Candida spp. and by growth kinetics assays. Toxicity was verified using in vitro assays of hemolysis, osmotic fragility, oxidant and antioxidant activity in human erythrocytes and by in vivo acute systemic toxicity in Galleria mellonella larvae. Fourteen different compounds were identified in Sc, which showed antifungal activity (MIC between 31.25-125 μg/mL) with fungistatic effect on Candida. At antifungal concentrations, it demonstrated low cytotoxicity, antioxidant activity and neglible in vivo toxicity. Thus, Sc demonstrated a promising antifungal potential, with low toxicity, indicating that this extract can be a safe and effective alternative antifungal agent.

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Curcumin micronization by supercritical fluid: in vitro and in vivo biological relevance

10.1101/2021.07.08.451641 ◽

2021 ◽

Author(s):

Adrieli Sachett ◽

Matheus Gallas-Lopes ◽

Radharani Benvenutti ◽

Matheus Marcon ◽

Gean Pablo S. Aguiar ◽

...

Keyword(s):

Antioxidant Activity ◽

Curcuma Longa ◽

Antioxidant Properties ◽

Behavioral Effects ◽

Antioxidant Power ◽

Zebrafish Brain ◽

Protein Thiols ◽

Tank Test

Curcumin, a polyphenol extracted from the rhizome of Curcuma longa L. (Zingiberaceae), is shown to have antioxidant, anti-inflammatory, neuroprotective, anxiolytic, and antidepressant properties in both preclinical and clinical studies. However, its low bioavailability is a limitation for its potential adoption as a therapeutic agent. The process of micronization can overcome this barrier by reducing the particle size and increasing the dissolution rate, potentially improving the bioavailability of the compounds of interest. In this study, we compared the in vitro antioxidant effects of curcumin (CUR) and micronized curcumin (MC) and studied their effects on behavioral and neurochemical parameters in zebrafish submitted to unpredictable chronic stress (UCS). MC (1 g/L) presented higher antioxidant activity in vitro as compared to CUR, as measured by iron-reducing antioxidant power (FRAP), 1,1-diphenyl-2-2-picyryl-hydrazyl radical removal (DPPH), and deoxyribose tests. UCS increased total distance traveled in the social interaction test (SI), while decreased crossings, time, and entries to the top area in the novel tank test (NTT). No effects of UCS were observed in the open tank test (OTT). The behavioral effects induced by UCS were not blocked by any curcumin preparation. UCS also decreased non-protein thiols (NPSH) levels, while increased glutathione reductase (GR) activity and thiobarbituric acid reactive substances (TBARS) levels on zebrafish brain. MC presented superior antioxidant properties than CUR in vivo, blocking the stress-induced neurochemical effects. Although this study did not measure the concentration of curcumin on the zebrafish brain, our results suggest that micronization increases the bioavailability of curcumin, potentiating its antioxidant activity both in vitro and in vivo. Our study also demonstrates that counteracting the oxidative imbalance induced by UCS is not sufficient to block its behavioral effects.

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Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

Revista de Chimie ◽

10.37358/rc.19.6.7243 ◽

2019 ◽

Vol 70 (6) ◽

pp. 1903-1907

Author(s):

Roxana Liana Stan ◽

Bogdan Sevastre ◽

Adriana Corina Hangan ◽

Sanda Bota ◽

Daniela Hanganu ◽

...

Keyword(s):

Antioxidant Activity ◽

Artemisia Annua ◽

Histopathological Examination ◽

Haematological Toxicity ◽

Chemical Characterization ◽

In Vivo Toxicity ◽

Artemisia Annua L ◽

Toxicity Studies

The purpose of the study was to demonstrate in vitro antioxidant activity and in vivo toxicity of the Artemisia Annua L. extract. The plant was harvested from Bihor area (Crisul Repede and Negru river valleys), Romania. Preparation of the plant product and of the lyophilizated extract was carried out in accordance with the Romanian Pharmacopeia Xth Edition. Lyophilized extract was evaluated in terms of polyphenol content using HPLC method. Antioxidant activity was highlighted using the DPPH, ABTS and FRAP methods. Hepatic, renal and haematological toxicity studies have been performed on laboratory mice. For this purpose blood and organs were collected. Biochemical and haematological parameters were determined on the blood samples and histopathological examination was performed on organs. In vitro antioxidant effect of Artemisia Annua L extract and its lack of in vivo toxicity were demonstrated. It is desirable to obtain a new phytoproduct harvested from spontaneous flora of Romania with antioxidant / antitumoral properties and which is devoid of toxicity.

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COMPARATIVE STUDIES OF PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF IN VIVO PLANT AND IN VITRO CALLUS EXTRACT OF CARDIOSPERMUM HALICACABUM L.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i8.42197 ◽

2021 ◽

pp. 94-103

Author(s):

THANIARASU R ◽

LOGESHWARI M

Keyword(s):

Antioxidant Activity ◽

Biological Activities ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Dry Weight ◽

Biologically Active ◽

Diffusion Method ◽

Total Phenolic

Objective: The present investigation focuses on the use of Cardiospermum halicacabum L. in their phytochemical and biological activities. Methods: In this study, in vivo stem and in vitro callus ethanolic extracts of C. halicacabum were tested for their phytochemical attributes by qualitative method, Fourier transform infrared (FTIR), antioxidant, antibacterial, and bioactive compound properties. The bactericidal activity of the in vivo stem and in vitro callus extract has been evaluated in both Gram+ve and Gram-ve microorganisms using the disk diffusion method. Results: The highest frequency (78%) of well developed, dark green organogenic callus was induced from stem explant on Murashige and Skoog (MS) medium supplemented with 0.7 mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l benzyl adenine (BA). The results of FTIR spectra confirmed the presence of functional groups in wild stem and in vitro callus extract of C. halicacabum with various peaks. The total phenolic content in ethanolic extract of in vivo plant and in vitro callus was 80.46 mg gallic acid equivalent (GAE)/g dry weight and 76.4 mg GAE/g dry weight, respectively. The highest percentage of tannins was measured at 78.03 in wild stem ethanol extracts followed by 75.22 in callus extract. The antioxidant activity of 2,2-diphenyl-2- picrylhydrazyl (DPPH) ethanol extract was found to be 206.54 μg/ml. IC50 values of the stem extracts of C. halicacabum are 306 μg/ml and 286 μg/ml in callus extract, respectively. Antibacterial activity of the ethanol extract was higher for Staphylococcus aureus (S. aureus) with a 17 mm zone of inhibition. Conclusion: The present investigation recommended that the callus ethanolic extract function as a good source of biologically active compounds and natural antioxidants.

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In vivo and in vitro antioxidant effects of three Veronica species

Open Life Sciences ◽

10.2478/s11535-012-0041-4 ◽

2012 ◽

Vol 7 (3) ◽

pp. 559-568 ◽

Cited By ~ 4

Author(s):

Jelena Živković ◽

Tatjana Ćebović ◽

Zoran Maksimović

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Thiobarbituric Acid ◽

Butylated Hydroxytoluene ◽

Antioxidant Systems ◽

Antioxidant Power ◽

Antioxidant Effects

AbstractThe aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe2+/g, and DPPH IC50 values from 12.58 to 66.34 µg/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe2+/g; DPPH IC50: 9.57 µg/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl4). Pretreatment with 100 mg/kg b.w. of Veronica extracts inhibited CCl4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl4-induced liver damage by their antioxidative effect on hepatocytes.

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Acetone Extract fromRhodomyrtus tomentosa: A Potent Natural Antioxidant

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/535479 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 29

Author(s):

Goodla Lavanya ◽

Supayang Piyawan Voravuthikunchai ◽

Nongporn Hutadilok Towatana

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antioxidant Property ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Antioxidant Power ◽

Lipid Peroxidation Inhibition

Rhodomyrtus tomentosa(Myrtaceae) has been employed in traditional Thai medicine to treat colic diarrhoea, dysentery, abscesses, haemorrhage, and gynaecopathy. In addition, it has been used to formulate skin-whitening, anti-aging and skin beautifying agents. Ethnomedical activities of this plant may be due its antioxidant property. Hence, the aim of this study was to evaluate bothin vitroandin vivoantioxidant activities ofR. tomentosaleaf extract.In vitroantioxidant activity of the extract was assessed by lipid peroxidation inhibition capacity, ferric reducing antioxidant power, and metal chelating activity.R. tomentosaextract demonstrated its free radical scavenging effects in concentration dependent manner.In vivoantioxidant activity of the extract was conducted in SwissAlbinomice. Levels of thio-barbituric acid reactive substances (TBARS), glutathione (GSH), and the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in blood, liver, and kidney were analyzed using microtitre plate photometer. Administration of CCl4caused significant increase in TBARS and decrease in GSH, SOD, CAT and GPx levels. In contrast,R. tomentosaextract (0.8 g/kg) effectively prevented these alterations and maintained the antioxidant status. The results suggest thatR. tomentosaextract can serve as a potent antioxidant.

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