scholarly journals Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4113
Author(s):  
Byeong Ryeol Ryu ◽  
Md. Jahirul Islam ◽  
Md. Obyedul Kalam Azad ◽  
Eun-Ji Go ◽  
Md. Hafizur Rahman ◽  
...  
Keyword(s):  
High Performance ◽  
Cannabis Sativa ◽  
Raw Materials ◽  
Raw Material ◽  
Natural State ◽  
Gradient System ◽  
Uv Spectrum ◽  
Chromatography Method ◽  
Advanced Analysis ◽  
Liquid Chromatography Method

This study was carried out to develop a high-performance liquid chromatography method for short-time analysis of the main cannabinoids in the inflorescence of hemp (Cannabis sativa L.). We also performed decarboxylation of the raw material using our advanced analysis technique. In this study, the UV spectrum was considered to analyze each of the four common cannabinoids, solvents, and samples, where the uniform elution of acidic cannabinoids without peak tailing and acids was tested. Optimal results were obtained when readings were taken at a wavelength of 220 nm using water and methanol containing trifluoroacetic acid as mobile phases in a solvent gradient system. The established conditions were further validated by system suitability, linearity, precision, detection limit, and quantitation limit tests. The decarboxylation index (DT50) confirmed that Δ9-THCA decarboxylated faster than CBDA, and both maintained a linear relationship with time and temperature. In addition, the loss of cannabidiol was better prevented during the decarboxylation process in the natural state than in the extracted state.

scholarly journals Extraction and characterization of yeast extract bioethanol byproduct from empty palm oil bunch for raw material of cosmetic products

2018 ◽  
Vol 67 ◽  
pp. 03040
Author(s):  
Muhamad Sahlan ◽  
Muhammad Saefuddin ◽  
Muryanto ◽  
Heri Hermansyah ◽  
Anondho Wijanarko
Keyword(s):  
Yeast Extract ◽  
Raw Materials ◽  
Raw Material ◽  
Yeast Fermentation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chromatography Method ◽  
Column Adsorption ◽  
Liquid Chromatography Method ◽  
Feasibility Test ◽  
Placental Extract

Ethanolic fermentation can produce byproducts such as yeast containing intracellular amino acid that is used as a raw material of cosmetics. Residual yeast fermentation as sludge was dissolved and extracted by autolysis at 50°C for 24 hours, so we get the product in the form of intracellular content of the yeast Saccharomyces cerevisiae. Purification of dye and odor yeast extract was conducted by using an activated carbon column adsorption with ratio 1.5:10 yeast extract solution (g / mL) for six times recycle or until it reaches the absorbance value of 0.020. The content of yeast extract in the form of amino acids was analyzed by High-Pressure Liquid Chromatography method. Analysis of the feasibility test yeast extract as cosmetic raw materials made through the pigment deposition method by inhibit tyrosinase activity. 0.05 g yeast extract before adsorption (pale yellow) produce 62% inhibition of tyrosinase 3130 U / mL. Dry yeast extract after adsorption (odorless) had 96% inhibition of tyrosinase 313 U / mL, whereas placental extract by 89% inhibition of tyrosinase 313 U / mL. These results indicate odorless yeast extract can replace placental extract as an alternative to cosmetic raw materials.

Isolation and Determination of Phenolic Glycosides and Anthraquinones from Rhizomes of Various Reynoutria Species

Planta Medica ◽  
2018 ◽  
Vol 84 (15) ◽  
pp. 1118-1126 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Sebastian Granica ◽  
Krzysztof Domaradzki ◽  
Łukasz Pecio ◽  
Adam Matkowski
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Reversed Phase ◽  
Array Detector ◽  
Sucrose Esters ◽  
Chromatography Method ◽  
Reynoutria Japonica ◽  
Flight Mass Spectrometry ◽  
Liquid Chromatography Method ◽  
Reynoutria Sachalinensis

AbstractGiant knotweeds of the genus Reynoutria (syn. Fallopia)–Reynoutria japonica, Reynoutria sachalinensis, and a hybrid of them, Reynoutria x bohemica–are noxious invasive plants in Europe and North America. R. japonica is a traditional East Asian (Japan and China) drug (Polygoni cuspidati rhizoma). Recently, it has been included in European Pharmacopoeia as one of the traditional Chinese medicinal herbs. In this study, a reversed-phase high performance liquid chromatography method with diode array detector and time-of-flight mass spectrometry was developed and validated for the profiling of rhizomes from European invasive populations and Polygoni cuspidati rhizoma purchased in China. Twenty-five compounds were identified, mainly stilbenes, anthraquinones, flavan-3-ols, and phenylpropanoid esters. Tatariside B, hydropiperoside, vanicoside C, a new compound (3,6-O-di-p-coumaroyl)-β-fructofuranosyl-(2 → 1)-(2′-O-acetyl-6′-O-feruloyl)-β-glucopyranoside) were reported for the first time in these raw materials. Six compounds from three phytochemical classes–stilbenes: piceid and resveratrol; anthraquinones: emodin and physcion; hydroxycinnamic sucrose esters: vanicosides A and B–were quantified using the validated method. R. japonica from China contained twice as many stilbenoids than samples from Poland (piceid 14.83 mg/g dm vs. 7.45 mg/g and resveratrol 1.29 mg/g vs. 0.65 mg/g). R. sachalinensis rhizomes contained lower quantities of anthraquinones and no detectable stilbenes, which together with higher amounts of hydroxycinnamic glycosides makes it easily distinguishable from the other two. The phytochemical profile of R. x bohemica was intermediate between the two parent species.

scholarly journals QUANTITATIVE DETERMINATION OF ARTEANNUIN B – A SESQUITERPENE LACTONE FROM ARTEMISIA ANNUA

2021 ◽  
pp. 163-170
Author(s):  
Rimma Fail'yevna Mukhamatkhanova ◽  
Vyacheslav Vadimovich Uzbekov ◽  
Dilnoza Karimberdievna Mutalova ◽  
Munirakhon Akhmatkhon Kizi Mamatkhanova ◽  
Il'dar Dzhamil'yevich Sham’yanov ◽  
...  
Keyword(s):  
High Performance ◽  
Standard Sample ◽  
Separation Efficiency ◽  
Artemisia Annua ◽  
Raw Material ◽  
Chromatography Method ◽  
Marker Compound ◽  
Liquid Chromatography Method ◽  
System Separation

The HPLC (High performance liquid chromatography) method for the determination of arteannuin B in the herb Artemisia annua has been developed. The method is suitable for its validation characteristics, such as specificity parameters (convergence of the retention times of artianuin B in the standard and sample), suitability of the chromatographic system (separation efficiency), linearity (R2 = 0.99929 at y = 9.38x + 245.98), range of application (from 50 to 150%), accuracy in terms of repeatability (RSD = 3.45%) and correctness (Z = 100.36 ± 0.56%), determined experimentally, are within the recommended values. As a marker compound, it was proposed to use a standard sample of arteannuin B. For this, a standard sample of arteannuin B was obtained with a purity of at least 99%, which is confirmed IR, 1HNMR, 13CNMR spectral data. Organoleptic and physicochemical parameters were determined. It has been established that the presence of impurities in an amount of not more than 1% is allowed in a standard sample of arteannuin B. It was established that the content of arteannuin B in the raw material should be at least 0.2%. The development and validation HPLC-UV method for the determination of arteannuin B have to used to standardization the herb of Artemisia annua.

scholarly journals Analytical difficulties for determination of acesulfame K in chocolate products

Pharmacia ◽  
2020 ◽  
Vol 67 (2) ◽  
pp. 105-110
Author(s):  
Svetla Petrova ◽  
Valentina Christova-Bagdassarian
Keyword(s):  
High Performance ◽  
Spectral Characteristics ◽  
Uv Spectrum ◽  
Reverse Phase ◽  
Chromatography Method ◽  
Free Products ◽  
Cocoa Products ◽  
Confectionery Products ◽  
Liquid Chromatography Method

Sweeteners are substances used as a dietary supplement to replace sugar. Consumers are concerned about the high levels of sugar, calories and cariogenicity in confectionery products, which is why the popularity of the so-called. „Light“ products and „sugar-free“ products. Acesulfame K is a synthetic sweetener about 200 times sweeter than sugar. In the present work, an analysis of acesulfame K in cocoa and chocolate products was performed. For the determination of sweeteners acesulfame K, saccharin and aspartame in foodstuffs, a standardized reverse phase high performance liquid chromatography method with UV detection was used. A cocoa matrix-specific compound was observed in all chocolate products analyzed for acesulfame K. Interference did not correspond to acesulfame K on the UV spectrum and could not be removed by two-step purification. The comparison of the spectral characteristics allowed to avoid a misleading result for the presence of acesulfame K in chocolate and cocoa products.

scholarly journals Development and Validation of a Column High-Performance Liquid Chromatography Method for Quantification of Ganaphaliin A and B in Inflorescences of Gnaphalium liebmannii Sch. Bp ex Klatt

2011 ◽  
Vol 94 (4) ◽  
pp. 1076-1081 ◽  
Author(s):  
Fernando Rodríguez-Ramos ◽  
Víctor H Sánchez-Estrada ◽  
Alejandro Alfaro-Romero ◽  
Gabriela Rubí Tapia-Álvarez ◽  
Andrés Navarrete
Keyword(s):  
High Performance ◽  
Hplc Method ◽  
Raw Material ◽  
Array Detector ◽  
Chromatography Method ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Liquid Chromatography Method ◽  
Development And Validation

Abstract An HPLC method was developed for the simultaneous determination of gnaphaliin A and B, active compounds of Gnaphalium liebmannii Sch. Bp ex Klatt. The HPLC separation was performed on an Inertsil ODS-3 (150 × 4.6 mm id, 5 μm) RP C18 column operated at 40°C; the isocratic mobile phase was 0.02% aqueous orthophosphoric acid– methanol–acetonitrile (50 + 30 + 20, v/v/v), with a run time of 20 min and flow rate of 1.5 mL/min. Detection with a photodiode array detector (PDAD) was at 270 nm. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ for gnaphaliin A and B were found to be in the range of 0.4–0.5 and 1.0–1.4 μg/mL, respectively. This is the frst report of an analytical method developed for the quantitative analysis of flavones from Gnaphalium species by HPLC-PDAD with applications for raw material and commercial products.

scholarly journals HPLC Quantification of Cytotoxic Compounds fromAspergillus niger

2017 ◽  
Vol 2017 ◽  
pp. 1-7
Author(s):  
Paula Karina S. Uchoa ◽  
Leandro Bezerra de Lima ◽  
Antonia T. A. Pimenta ◽  
Maria da Conceição F. de Oliveira ◽  
Jair Mafezoli ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
High Performance ◽  
Chromatography Method ◽  
Relative Standard ◽  
Validation Parameters ◽  
Cytotoxic Compounds ◽  
Liquid Chromatography Method ◽  
Marine Strain

A high-performance liquid chromatography method was developed and validated for the quantification of the cytotoxic compounds produced by a marine strain ofAspergillus niger. The fungus was grown in malt peptone dextrose (MPD), potato dextrose yeast (PDY), and mannitol peptone yeast (MnPY) media during 7, 14, 21, and 28 days, and the natural products were identified by standard compounds. The validation parameters obtained were selectivity, linearity (coefficient of correlation > 0.99), precision (relative standard deviation below 5%), and accuracy (recovery > 96).

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